Team:Brasil-SP/Notebook

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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Brasil-SP/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:Brasil-SP"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Brasil-SP"style="color:#000000"> Official Team Profile </a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Brasil-SP/Modeling"style="color:#000000"> Modeling</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Notebook"style="color:#000000"> Notebook</a></td>
 
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<a href="https://2014.igem.org/Team:Brasil-SP/Safety"style=" color:#000000"> Safety </a></td>
 
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<body>
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<a href="https://2014.igem.org/Team:Brasil-SP/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<h1 align=center>Main Assembly Map</h1>
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<p><div align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.</div></p>
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<a class="map_link" id="map_link_2" title="" href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>
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<a class="map_link" id="map_link_3" title="" href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a>
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<tr><td colspan="3"> <h3>Notebook</h3></td></tr>
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
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<h1>Main Assembly Map</h1>
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<!--estou colocando os fichamentos dos assemblies aqui, ainda preciso acabar os que faltam mas ja da pra experimentar com a organização destes-->
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<h1>Assemblies forms</h1>
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<p><strong>Assembly form template</strong></p>
<p><strong>Assembly form template</strong></p>
<p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p>
<p>This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)</p>
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<a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a>
<a href="https://static.igem.org/mediawiki/2014/e/eb/Assembly_Form.pdf"><img src="https://static.igem.org/mediawiki/2014/3/32/Assembly_form_pg3.png" style="width:700px;height:400px"/></a>
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<h1 align=center><strong>Life Inside the LAB</strong></h1>
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      <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf"><img src="https://static.igem.org/mediawiki/2014/e/e1/AII_image.png" width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf"><img src="https://static.igem.org/mediawiki/2014/a/ad/AIII_image.png" width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/e/eb/AIV.pdf"><img src="https://static.igem.org/mediawiki/2014/4/49/AIV_image.png" width="100%" height="150px"/></a>
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<h1>Characterization Assemblies</h1>
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<p> Apart from the main genetic circuit we also assembled others for characterization purposes, such as the validation of the promoters and tunning of our threshold setter concentration, the QteE.</p>
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<h2>Promoter BBa_K823003</h2>
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<p><strong>Question</strong>: Does the constitutive promoter BBa_K823003 work properly?</p>
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<p><strong>Results</strong>: After the incubation period of the transformed <em>E. coli</em> a large portion of the colonies were glowing green. So the promoter does work. Moreover, this biobrick works on <em>E. coli</em> despite the fact it was designed for <em>B. subtilis</em>.</p>
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<h2>Promoter BBa_K143015</h2>
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<p><strong>Question</strong>: How does the transcription caused by this promoter varies with the IPTG induction?</p>
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<p><strong>Results</strong>:</p>
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<h2>Tunning of the QteE Threshold</h2>
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<p><strong>Question</strong>:What are the concentration of QteE needed to hamper the LasR induction of the promoter PlasR?</p>
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<p>This is the most difficult task of our project. Tunning the production of QteE so that we establish the correct threshold for the discretization of the Cystatin C level in serum. To attack this challenge we designed 3 circuits so that we could plot a calibration curve. In this circuits we put the transcription of the LasR and QteE under two differnt promoters, the Pveg (BBa_K823003) and PlasR (BBa_K143015).</p>
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<h1>Assembly Forms<h1>
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      <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf"><img src="https://static.igem.org/mediawiki/2014/2/23/KI.png"width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf"><img src="https://static.igem.org/mediawiki/2014/c/c5/KII.png"width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf"><img src="https://static.igem.org/mediawiki/2014/e/ed/KIII.png"width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf"><img src="https://static.igem.org/mediawiki/2014/e/ee/KIV.png"width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf"><img src="https://static.igem.org/mediawiki/2014/3/37/KVI.png"width="100%" height="150px"/></a>
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      <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf"><img src="https://static.igem.org/mediawiki/2014/b/b8/KXI.png"width="100%" height="150px"/></a>
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      <li><a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a></li>
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      <li><a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a></li>
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+
-
      <li><a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a></li
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/b/b3/KII.pdf">KII</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/7/78/KIII.pdf">KIII</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/9/95/KIV.pdf">KIV</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/3/3d/KV.pdf">KV</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/9/95/KX.pdf">KX</a></li>
+
-
      <li><a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a></li>
+
-
  </ul>
+
-
-->
+
-
<h1><strong>Life Inside the LAB</stron></h1>
+
-
<thead><h2>July</h2>
+
<thead><h2 align=center>July</h2>
</thead>
</thead>
Line 326: Line 117:
<table>
<table>
   <tr>
   <tr>
-
     <th>Monday</th>  
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <th>Tuesday</th>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <th>Wednesday</th>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <th>Thursday</th>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <th>Friday</th>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <th>Saturday</th>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <th>Sunday</th>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td>30/06
     <td>30/06
     <ul>
     <ul>
-
       <li>PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion.</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li>
     </ul>
     </ul>
     </td>
     </td>
     <td>01/07  
     <td>01/07  
     <ul>
     <ul>
-
       <li>Purification of lasR PCR product </li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of lasR <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> product </li>
     </ul>
     </ul>
     </td>
     </td>
     <td>02/07
     <td>02/07
     <ul>
     <ul>
-
       <li>Transformation of the Biobricks:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
-
         <li>BBa_E0840</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
       </ul>
       </ul>
       <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
       <li>Inoculum of the Biobricks sent by iGEM HQ:</li>
       <ul>
       <ul>
-
         <li>BBa_K316037</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
-
         <li>BBa_K316018</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
-
         <li>BBa_K316015</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 362: Line 153:
     <td>03/07
     <td>03/07
     <ul>
     <ul>
-
       <li>Transformation of the Biobricks sent by the Imperial College Team:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the Biobricks sent by the Imperial College Team:</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016<a/></li>
-
         <li>BBa_K143031</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143031" style="color:#3ab473">BBa_K143031<a/></li>
       </ul>
       </ul>
-
       <li>Miniprep, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification of the plasmidial DNA samples and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316018" style="color:#3ab473">BBa_K316018<a/></li>
-
         <li>BBa_E0840</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316015" style="color:#3ab473">BBa_K316015<a/></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 381: Line 172:
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
-
         <li>BBa_E0840</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 391: Line 182:
     <td>07/07
     <td>07/07
     <ul>
     <ul>
-
       <li>Miniprep, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_143012</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143012" style="color:#3ab473">BBa_K143012</a></li>
-
         <li>BBa_K081001</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K081001" style="color:#3ab473">BBa_K081001</a></li>
-
         <li>BBa_E0840</li>
+
         <li<a href="http://parts.igem.org/Part:BBa_E0840" style="color:#3ab473">BBa_E0840</a></li>
       </ul>
       </ul>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 408: Line 199:
     <li>Inoculum:</li>
     <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 414: Line 205:
     <td>10/07
     <td>10/07
     <ul>
     <ul>
-
       <li>Miniprep, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, Quantification and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015 (restriction analysis fail, repeat)</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a> (restriction analysis fail, repeat)</li>
       </ul>
       </ul>
-
       <li>PCR the qteE gene for amplification. We also added the RBS (BBa_K143021) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion.</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> the qteE gene for amplification. We also added the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021,</a>) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called <a href="http://parts.igem.org/Part:BBa_K1521000" style="color:#3ab473">BBa_K1521000</a>.</li>
-
       <li>Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of RBS+lasR in PTZ57R/T vector (instaclone kit)</li>
     </ul>
     </ul>
     </td>
     </td>
Line 425: Line 216:
     <ul>
     <ul>
       <li>Prepare 40% glycerol solution</li>
       <li>Prepare 40% glycerol solution</li>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>RBS + lasR (BBa_C0079)</li>
+
         <li>RBS + lasR (<a href="http://parts.igem.org/Part:BBa_C0079"style="color:#3ab473">BBa_C0079</a>)</li>
       </ul>
       </ul>
-
       <li>Purification:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
       <ul>
       <ul>
         <li>RBS + qteE</li>
         <li>RBS + qteE</li>
Line 455: Line 246:
       </ul>
       </ul>
       <li>Replate RBS+lasR</li>
       <li>Replate RBS+lasR</li>
-
       <li>Ligation of PCR qteE in pGEM T-easy vector</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of <a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> qteE in pGEM T-easy vector</li>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> <a href="" style="color:#3ab473"> BBa_J04450</a></a></li>
       </ul>
       </ul>
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 468: Line 259:
     <td>16/07
     <td>16/07
     <ul>
     <ul>
-
     <li>Tranformation:</li>
+
     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a>:</li>
       <ul>
       <ul>
-
       <li>BBa_K823003</li>
+
       <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
       <li>RBS+qteE</li>
       <li>RBS+qteE</li>
       </ul>
       </ul>
     <li>Electrophoresis analysis:</li>
     <li>Electrophoresis analysis:</li>
       <ul>
       <ul>
-
       <li>BBa_B0015</li>
+
       <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 481: Line 272:
     <td>17/07
     <td>17/07
     <ul>
     <ul>
-
       <li>Glycerol Stock and Miniprep:<li>
+
       <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:<li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
         <li>RBS+lasR</li>
         <li>RBS+lasR</li>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450 (EP)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
-
         <li>BBa_J04450 (XS)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
       </ul>
       </ul>
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_K823003</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></li>
       </ul>
       </ul>
-
       <li>Transformation</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       <ul>
       <ul>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 504: Line 295:
     <td>18/07
     <td>18/07
     <ul>
     <ul>
-
       <li>Glycerol Stock and Miniprep:</li>
+
       <li>Glycerol Stock and <a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
-
         <li>BBa_B0015</li>
+
         <li><a href"http://parts.igem.org/Part:BBa_B0015" style="color:#3ab473">BBa_B0015</a></li>
       </ul>
       </ul>
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_J04450 (EP)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (EP)</li>
-
         <li>BBa_J04450 (XS)</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a> (XS)</li>
       </ul>
       </ul>
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_K823003</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K823003" style="color:#3ab473">BBa_K823003</a></a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 525: Line 316:
     <td>21/07
     <td>21/07
     <ul>
     <ul>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
       </ul>
       </ul>
Line 544: Line 335:
       <li>Inoculum:</li>
       <li>Inoculum:</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
-
       <li>Miniprep, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>, <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a>:</li>
       <ul>
       <ul>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
Line 553: Line 344:
       <ul>
       <ul>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 561: Line 352:
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/d6/AIII.pdf">AIII</a> and <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
-
       <li>Prepare more competent cells</li>
+
       <li>Prepare more <a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf" style="color:#f05151"> competent cells</a></li>
       <li>Prepare LB medium (solid and liquid)</li>
       <li>Prepare LB medium (solid and liquid)</li>
       <li>Inoculum</li>
       <li>Inoculum</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
       <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
       <li>Prepare RBS+lasR and RBS+qteE for sequencing</li>
Line 578: Line 369:
         <li>Inoculum</li>
         <li>Inoculum</li>
       </ul>
       </ul>
-
       <li>Miniprep:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_K316016</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 589: Line 380:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
Line 602: Line 393:
       <ul>
       <ul>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (+NdeI enzyme)</li>
-
         <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that bot the construction and the pSB1C3 vector had similar size. Check out the Lab Journal report of the day for more information.</li>
+
         <li>OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.</li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
Line 619: Line 410:
       <ul>
       <ul>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 629: Line 420:
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/d/d6/AVI.pdf">AVI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>     
     </ul>     
Line 641: Line 432:
         <li>Inoculum</li>
         <li>Inoculum</li>
       </ul>
       </ul>
-
       <li>PCR BBa_K143055 for RBS removal</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for RBS removal</li>
       <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
       <li>OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this  Lac promotor :) </li>
-
       <li>PCR of Plac BBa_K143055 was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and Ligation in the PUC19 vector</li>
+
       <li>PCR of Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> was followed by a <a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> in the PUC19 vector</li>
     </ul>
     </ul>
     </td>
     </td>
Line 651: Line 442:
   </tr>
   </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
</tbody>
</tbody>
-
<thead><h2>August</h2>
+
<thead><h2 align=center>August</h2>
</thead>
</thead>
Line 662: Line 458:
<table style="width:100%">
<table style="width:100%">
   <tr>
   <tr>
-
     <th>Monday</th>
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <th>Tuesday</th>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <th>Wednesday</th>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <th>Thursday</th>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <th>Friday</th>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <th>Saturday</th>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <th>Sunday</th>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td></td>
     <td></td>
Line 676: Line 472:
     <td>01/08
     <td>01/08
     <ul>
     <ul>
-
       <li>Transformation:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/3/38/AII.pdf">AII</a>,
Line 685: Line 481:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
Line 700: Line 496:
         <li>E. coli DH5-alpha</li>
         <li>E. coli DH5-alpha</li>
         <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a></li>
-
         <li>Plac BBa_K143055</li>
+
         <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
-
       <li>Transformation</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       <ul>
       <ul>
-
         <li>BBa_P0312</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
Line 716: Line 512:
     <ul>
     <ul>
       <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
       <li>Prepare assemblies <a href="https://static.igem.org/mediawiki/2014/9/90/KI.pdf">KI</a> for cytometry characterization</li>
-
       <li>Miniprep:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a>:</li>
       <ul>
       <ul>
-
         <li>Plac BBa_K143055</li>
+
         <li>Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
       <li>Inoculum</li>
       <li>Inoculum</li>
       <ul>
       <ul>
-
         <li>BBa_P0312</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
       </ul>
       </ul>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/f/fe/AV.pdf">AV</a>:</li>
       <ul>
       <ul>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
-
         <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this BBa_K316016 is tough)</li>
+
         <li>And once again! <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a> (this <a href="http://parts.igem.org/Part:BBa_K316016" style="color:#3ab473">BBa_K316016</a> is tough)</li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 734: Line 530:
     <td>07/08
     <td>07/08
     <ul>
     <ul>
-
       <li>Miniprep and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a>:</li>
       <ul>
       <ul>
-
         <li>BBa_P0312</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_P0312" style="color:#3ab473">BBa_P0312</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 746: Line 542:
     <td>11/08
     <td>11/08
     <ul>
     <ul>
-
       <li>Prepare Plac BBa_K143055 for sequencing</li>
+
       <li>Prepare Plac <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> for sequencing</li>
       <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
       <li>Analyse the RBS+qteE and RBS+lasR sequencing file</li>
     </ul>
     </ul>
Line 771: Line 567:
     <td>14/08
     <td>14/08
     <ul>
     <ul>
-
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and Ligation in pSB1C3 for BBiobrick standard:</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> and <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a> in pSB1C3 for BBiobrick standard:</li>
       <ul>
       <ul>
         <li>RBS+qteE</li>
         <li>RBS+qteE</li>
Line 809: Line 605:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
       <li>Prepare LB medium (liquid and solid)</li>
       <li>Prepare LB medium (liquid and solid)</li>
Line 819: Line 615:
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/e/ea/BIII.pdf">BIII</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 838: Line 634:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
Line 848: Line 644:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/4/48/BII.pdf">BII</a>:</li>
       <ul>
       <ul>
-
         <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI (for more info check out the Lab Journal)</li>
+
         <li>Repeat <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">restriction analysis</a> adding the enzyme NdeI.</li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 859: Line 655:
       <li>Inoculum</li>
       <li>Inoculum</li>
       <ul>
       <ul>
-
         <li>BBa_J04450</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_J04450" style="color:#3ab473"> BBa_J04450</a></li>
-
         <li>BBa_K143055</li>
+
         <li><a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a></li>
       </ul>
       </ul>
-
       <li>PCR BBa_K143055 with higher melting temperature (check out our Lab Journal for more info)</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> with higher melting temperature.</li>
     <ul>
     <ul>
     </td>
     </td>
Line 895: Line 691:
   </tr>
   </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
-
<thead><h2>September</h2>
+
<thead><h2 align=center>September</h2>
</thead>
</thead>
Line 904: Line 703:
<table style="width:100%">
<table style="width:100%">
   <tr>
   <tr>
-
     <td>Monday</td>
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <td>Tuesday</td>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <td>Wednesday</td>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <td>Thursday</td>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <td>Friday</td>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <td>Saturday</td>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <td>Sunday</td>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td>01/09
     <td>01/09
Line 916: Line 715:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/a/a3/CII.pdf">CII</a>
       <ul>
       <ul>
-
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV (for more info check out our Lab Journal)
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion</a> of the part BII with EcoRV.
       </ul>
       </ul>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/b/b7/AI.pdf">AI</a>,  
Line 924: Line 723:
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
       <ul>
       <ul>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 936: Line 735:
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
         <a href="https://static.igem.org/mediawiki/2014/8/83/KVI.pdf">KVI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 961: Line 760:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
Line 973: Line 772:
     <td>08/09
     <td>08/09
     <ul>
     <ul>
-
       <li>QuickChange PCR to mutate the cleavage site of the BBa_316037 to a Cathepsin S recognition site</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
-
       <li>Transformation of the QuickCHange PCR product</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
     </ul>
     </ul>
     </td>
     </td>
Line 981: Line 780:
     <ul>
     <ul>
       <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
       <li>The QuickChange protocol didn't work well. So try again with more attention!</li>
-
       <li>QuickChange PCR to mutate the cleavage site of the BBa_316037 to a Cathepsin S recognition site</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf" style="color:#f05151">QuickChange PCR</a> to mutate the cleavage site of the <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037</a> to a Cathepsin S recognition site</li>
-
       <li>Transformation of the QuickCHange PCR product</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a> of the QuickCHange PCR product</li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
Line 994: Line 793:
     <td>11/09
     <td>11/09
     <ul>
     <ul>
-
       <li>Prepare inoculum of the mutated BBa_K316037</li>
+
       <li>Prepare inoculum of the mutated <a href="http://parts.igem.org/Part:BBa_K316037" style="color:#3ab473">BBa_K316037<a/></li>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
           <a href="https://static.igem.org/mediawiki/2014/c/ce/KVIII.pdf">KVIII</a>,  
Line 1,001: Line 800:
       <ul>
       <ul>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>   
       </ul>   
     </ul>
     </ul>
Line 1,012: Line 811:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Tranformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151">Transformation</a></li>
       </ul>
       </ul>
-
       <li>We had far to many difficulties trying to remove the RBS from the BBa_K143055, so we decided to synthesize it using PCR.</li>
+
       <li>We had far to many difficulties trying to remove the RBS from the <a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a>, so we decided to synthesize it using PCR.</li>
-
       <li>PCR for BBa_143015 (BBa_143055 without RBS)</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf" style="color:#f05151">PCR for synthesis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> (<a href="http://parts.igem.org/Part:BBa_K143055" style="color:#3ab473">BBa_K143055</a> without RBS)</li>
     </ul>
     </ul>
     </td>
     </td>
Line 1,028: Line 827:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Repeat Ligation</li>
+
         <li>Repeat <a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
       </ul>
       </ul>
       <li>Prepare LB medium (solid and liquid)</li>
       <li>Prepare LB medium (solid and liquid)</li>
Line 1,038: Line 837:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
       </ul>
       </ul>
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/7/72/DI.pdf">DI</a>,  
Line 1,044: Line 843:
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
           <a href="https://static.igem.org/mediawiki/2014/9/9c/KXI.pdf">KXI</a>:</li>
       <ul>
       <ul>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 1,061: Line 860:
         <li>Inoculum</li>
         <li>Inoculum</li>
       </ul>
       </ul>
-
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of the BBa_K143015</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
     </ul>       
     </ul>       
     </td>
     </td>
Line 1,072: Line 871:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
Line 1,079: Line 878:
     <td>17/09
     <td>17/09
     <ul>
     <ul>
-
       <li>LIgation of the synthesized BBa_K143015 in TOPO vector followed by Transformation</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Clonig.pdf" style="color:#f05151">Ligation</a> of the synthesized <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a> in TOPO vector followed by <a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
     </ul>
     </ul>
Line 1,089: Line 888:
     <td>18/09
     <td>18/09
     <ul>
     <ul>
-
       <li>Replate BBa_K143015. We forgot the X-gal :(</li>
+
       <li>Replate <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a>. We forgot the X-gal :(</li>
     </ul>
     </ul>
     <ul>
     <ul>
Line 1,101: Line 900:
     <td>22/09
     <td>22/09
     <ul>
     <ul>
-
       <li>Inoculum of the BBa_K143015</li>
+
       <li>Inoculum of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
       <ul>
       <ul>
-
         <li>Digestão EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 1,113: Line 912:
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
       <li>Assembly <a href="https://static.igem.org/mediawiki/2014/6/67/KXIV.pdf">KXIV</a>:</li>
       <ul>
       <ul>
-
         <li>Digestão EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
       </ul>
       </ul>
-
       <li>Miniprep and Rstriction Analysis of the BBa_K143015</li>
+
       <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf">Miniprep</a> and <a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction Analysis</a> of the <a href="http://parts.igem.org/Part:BBa_K143015" style="color:#3ab473">BBa_K143015</a></li>
     </ul>
     </ul>
     </td>
     </td>
Line 1,123: Line 922:
       <ul>
       <ul>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 1,141: Line 940:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
Line 1,162: Line 961:
       <ul>
       <ul>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
-
         <li>Ligation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
-
         <li>Transformation</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 1,184: Line 983:
   </tr>
   </tr>
</table>
</table>
 +
<br>
 +
<br>
 +
<br>
 +
 +
-
<thead><h2>October</h2>
+
<thead><h2 align=center>October</h2>
</thead>
</thead>
Line 1,193: Line 997:
<table style="width:100%">
<table style="width:100%">
   <tr>
   <tr>
-
     <td>Monday</td>
+
     <th style="background-color:#bfacc0">Monday</th>  
-
     <td>Tuesday</td>
+
     <th style="background-color:#bfacc0">Tuesday</th>
-
     <td>Wednesday</td>
+
     <th style="background-color:#bfacc0">Wednesday</th>
-
     <td>Thursday</td>
+
     <th style="background-color:#bfacc0">Thursday</th>
-
     <td>Friday</td>
+
     <th style="background-color:#bfacc0">Friday</th>
-
     <td>Saturday</td>
+
     <th style="background-color:#bfacc0">Saturday</th>
-
     <td>Sunday</td>
+
     <th style="background-color:#bfacc0">Sunday</th>
   </tr> <tr>
   </tr> <tr>
     <td></td>
     <td></td>
Line 1,210: Line 1,014:
       <ul>
       <ul>
         <li>Glycerol Stock</li>
         <li>Glycerol Stock</li>
-
         <li>Miniprep</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/6/6f/Purelink_quick_plasmid_qrc.pdf"style="color:#f05151">Miniprep</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
Line 1,228: Line 1,032:
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
-
       <li>Assemblies KIX, CIII and KXVI</li>
+
       <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <ahref="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
       <ul>
       <ul>
-
         <li>Digestão EXSP</li>
+
         <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Digestion EXSP</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>07/10 
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf"  style="color:#f05151">Gel Purification</a></li>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/7/7a/Cohesive-end_assembly_cloning.pdf" style="color:#f05151">Ligation</a></li>
 +
      </ul>
 +
    </ul>
 +
    </td>
 +
    <td>08/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf" style="color:#f05151"> Transformation</a></li>
 +
      </ul>
 +
    <ul>
 +
    </td>
 +
    <td>09/10
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li>Inoculum</li>
 +
      </ul>
 +
    </td>
 +
    <td>10/10
 +
    <ul>
 +
      <li>Assemblies <a href="https://static.igem.org/mediawiki/2014/d/da/KIX.pdf">KIX</a>,
 +
          <a href="https://static.igem.org/mediawiki/2014/5/5b/BIV.pdf">BIV</a> and
 +
          <a href="https://static.igem.org/mediawiki/2014/6/64/KXVI.pdf">KXVI</a></li>
 +
      <ul>
 +
        <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf" style="color:#f05151">Restriction analysis</a></li>
       </ul>
       </ul>
     </ul>
     </ul>
     </td>
     </td>
-
    <td>07/10</td>
 
-
    <td>08/10</td>
 
-
    <td>09/10</td>
 
-
    <td>10/10</td>
 
     <td>11/10</td>
     <td>11/10</td>
     <td>12/10</td>
     <td>12/10</td>
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</html>
</html>
 +
{{:Team:Brasil-SP/Templates/Footer}}

Latest revision as of 20:32, 17 October 2014

HeaderNotebookBRASILAP.png

Main Assembly Map

     The main assembly map describes all the constructions that would be done in the project, but some of them have not been performed in time of the iGEM deadline. The succesfully constructed assemblies is indicated by a green check point and the unsuccessfull constructions are indicated by a red "X". Click on these assemblies to access the lab form.

AI AII AIII BIII AIV BII CII DI AVI AV BIV

Assemblies forms

Assembly form template

This is the template designed to help with the laboratory organization during the assemble of biological parts. If you want to use the assembly method we used you can print the form and complete it for each construction. Hope it helps :)

Life Inside the LAB

July

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
30/06
  • PCR lasR (BBa_C0079). The purpose of this PCR was to add the RBS (BBa_K143021), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called BBa_K1521001.
 01/07 02/07 03/07 04/07 05/07 06/07
07/07 08/07 09/07 10/07
  • Miniprep, Quantification and Restriction Analysis:
    • BBa_B0015 (restriction analysis fail, repeat)
  • PCR the qteE gene for amplification. We also added the RBS (BBa_K143021,) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000.
  • Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)
 11/07 12/07 13/07
14/07 15/07 16/07 17/07 18/07 19/07 20/07
21/07 22/07 23/07 24/07 25/07 26/07 27/07
28/07
  • Assembly AIII:
    • Restriction analysis (+NdeI enzyme)
    • OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.
  • Assembly AII, AV and AVI:
 29/07 30/07 31/07
  • Assembly AII, AV and AVI:
    • Inoculum
  • PCR BBa_K143055 for RBS removal
  • OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :)
  • PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector



August

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/08 02/08 03/08
04/08 05//08 06/08 07/08 08/08 09/08 10/08
11/08
  • Prepare Plac BBa_K143055 for sequencing
  • Analyse the RBS+qteE and RBS+lasR sequencing file
 12/08
  • Digestion:
    • RBS+qteE
    • RBS+lasR
    • OBS: this digestion was performed so we clone this parts in the pSB1C3 vector to put them in Biobrick standard
 13/08 14/08
  • Gel Purification and Ligation in pSB1C3 for BBiobrick standard:
    • RBS+qteE
    • RBS+lasR
  • Assemblies BII and BIII:
    • Gel Purification
    • OBS: we had some problems here with the purification and we lost all our digestions, so we're repeating the digestion
 15/08 16/08 17/08
18/08 19/08 20/08
  • RBS+qteE, RBS+lasR and Assemblies BII and BIII:
    • Inoculum
 21/08 22/08 23/08 24/08
25/08 26/08 27/08 28/08 29/08 30/08 31/08



September

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/09 02/09 03/09 04/09 05/09 06/09 07/09
08/09 09/09 10/09 11/09 12/09 13/09
  • Assembly KX
    • Inoculum
  • Assemblies DI, KVIII and KXI:
  • Prepare LB medium (solid and liquid)
 14/09
15/09 16/09 17/09
  • Measurement Interlab Study - Samples growth biobrick devices 1, 2 and 3
 18/09
  • Measurement Interlab Study - Sample preparation and characterization by Flow Cytometry and Fluorometry
 19/09 20/09 21/09
22/09 23/09 24/09 25/09
  • Assembly KXIV:
    • Inoculum
 26/09 27/09 28/09
29/09 30/09



October

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
01/10 02/10 03/10 04/10 05/10
06/10 07/10 08/10 09/10
  • Assemblies KIX, BIV and KXVI
    • Inoculum
    		 10/10 11/10 12/10
    13/10 14/10 15/10 16/10 17/10 18/10 19/10
    20/10 21/10 22/10 23/10 24/10 25/10 26/10
    27/10 28/10 29/10 30/10 31/10

    Sponsors

    This project is part of iGEM 2014

    Wiki Links

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    Retrieved from "http://2014.igem.org/Team:Brasil-SP/Notebook"