Team:Berlin

From 2014.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 26: Line 26:
     <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a>         
     <a href="https://2014.igem.org/Team:Berlin/Safety" class="main-menue-links"><li>Safety</li></a>         
     <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a>
     <a href="https://2014.igem.org/Team:Berlin/Workshop" class="main-menue-links"><li>Workshop</li></a>
-
     <a href="https://2014.igem.org/Team:Berlin/Blog"><li>Blog</li></a>
+
     <a href="https://2014.igem.org/Team:Berlin/Blog" class="main-menue-links"><li>Blog</li></a>
     <a href="https://igem.org/Main_Page" class="igem-link"><li><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></li></a>
     <a href="https://igem.org/Main_Page" class="igem-link"><li><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></li></a>
 +
    <a style="margin-left:50px;" href="https://2014.igem.org/Team:Berlin" class="main-menue-links"><li style="font-size: 0.7em;">English</li></a>
 +
    <a href="https://2014.igem.org/Team:Berlin_es" class="main-menue-links"><li style="font-size: 0.7em;">Spanish</li></a>
 +
    <a href="https://2014.igem.org/Team:Berlin_cn" class="main-menue-links"><li style="font-size: 0.7em;">Chinese</li></a>
   </ul>
   </ul>
</div>
</div>
Line 42: Line 45:
     <li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li>
     <li><a href="https://2014.igem.org/Team:Berlin/Blog">Blog</a></li>
     <li><a href="https://igem.org/Main_Page" class="igem-link"><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></a></li>
     <li><a href="https://igem.org/Main_Page" class="igem-link"><img class="igem-logo" src="https://static.igem.org/mediawiki/2014/d/d8/Team_Berlin_igem_logo.png"></a></li>
-
   </ul>
+
<li>Language:</li>   
 +
<li><a href="https://2014.igem.org/Team:Berlin"><li style="font-size: 0.9em;">English</a></li>
 +
<li>    <a href="https://2014.igem.org/Team:Berlin_es" ><li style="font-size: 0.9em;">Spanish</a></li>
 +
<li>   <a href="https://2014.igem.org/Team:Berlin_cn"><li style="font-size: 0.9em;">Chinese</a></li> 
 +
</ul>
</div>
</div>
Line 147: Line 154:
           <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Team</h2>       
           <h2><img src="https://static.igem.org/mediawiki/2014/f/ff/Team_Berlin_igem_questionmark.png" alt="" class="teaser-icons hidden-xs" />Team</h2>       
              
              
-
           <p>The iGEM Team Berlin consists of 17 students from various universities in Berlin.
+
           <p>Our team is made up of 17 undergraduates and postgraduates from a wide range of disciplines as well as our supervisors. The first iGEM Team Berlin 2014 is an assembly of highly motivated students from different universitys.  
-
          Represented are the Technische Universität Berlin, University of Applied Sciences Berlin,
+
Due to the multidisciplinarity our team is able to find different strategies to solve complex problems in synthetic biology.
-
          Freie Universität as well as the Humboldt University. The team members come from diverse
+
Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is  
-
          disciplines. From biology, biotechnology, biochemistry, computer science, mathematics, mechanical
+
-
          engineering to the history of science and technology, everything is represented. Just the
+
-
          interdisciplinary nature of the team allows us to jointly develop solution strategies from
+
-
          different perspectives. Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is  
+
           located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).</p>
           located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).</p>
           <button type="button" class="btn btn-success btn-lg hidden-lg">More Information</button>   
           <button type="button" class="btn btn-success btn-lg hidden-lg">More Information</button>   
Line 273: Line 276:
       <img src="https://static.igem.org/mediawiki/2014/7/79/Team_Berlin_tuberlin_logo.png" class="img-responsive grayscale">
       <img src="https://static.igem.org/mediawiki/2014/7/79/Team_Berlin_tuberlin_logo.png" class="img-responsive grayscale">
     </div>
     </div>
 +
 +
    <div class="col-sm-2 col-sm-offset-1 col-xs-3">
 +
      <img src="https://static.igem.org/mediawiki/parts/c/cb/Team_Berlin_florian_renner_logo.png" class="img-responsive grayscale">
 +
    </div>   
   </div>   
   </div>   
    
    
Line 304: Line 311:
       </div>
       </div>
       <div class="footer-imprint">
       <div class="footer-imprint">
-
         ©2014 iGem Berlin, Layout/Umsetzung: Valerian Blos, Marius Wessel
+
         ©2014 iGem Berlin, Layout/Implementation: Valerian Blos, Marius Wessel
       </div>
       </div>
      
      
Line 444: Line 451:
-
// Grayscale
 
-
 
-
// On window load. This waits until images have loaded which is essential
 
-
$(window).load(function(){
 
-
 
-
// Fade in images so there isn't a color "pop" document load and then on window load
 
-
$(".grayscale").fadeIn(500);
 
-
 
-
// clone image
 
-
$('.grayscale').each(function(){
 
-
var el = $(this);
 
-
el.css({"position":"absolute"}).wrap("<div class='img_wrapper' style='display: inline-block'>").clone().addClass('img_grayscale').css({"position":"absolute","z-index":"998","opacity":"0"}).insertBefore(el).queue(function(){
 
-
var el = $(this);
 
-
el.parent().css({"width":this.width,"height":this.height});
 
-
el.dequeue();
 
-
});
 
-
this.src = grayscale(this.src);
 
-
});
 
-
 
-
// Fade image
 
-
$('.grayscale').mouseover(function(){
 
-
$(this).parent().find('img:first').stop().animate({opacity:1}, 500);
 
-
})
 
-
$('.img_grayscale').mouseout(function(){
 
-
$(this).stop().animate({opacity:0}, 1000);
 
-
});
 
-
});
 
-
 
-
// Grayscale w canvas method
 
-
function grayscale(src){
 
-
var canvas = document.createElement('canvas');
 
-
var ctx = canvas.getContext('2d');
 
-
var imgObj = new Image();
 
-
imgObj.src = src;
 
-
canvas.width = imgObj.width;
 
-
canvas.height = imgObj.height;
 
-
ctx.drawImage(imgObj, 0, 0);
 
-
var imgPixels = ctx.getImageData(0, 0, canvas.width, canvas.height);
 
-
for(var y = 0; y < imgPixels.height; y++){
 
-
for(var x = 0; x < imgPixels.width; x++){
 
-
var i = (y * 4) * imgPixels.width + x * 4;
 
-
var avg = (imgPixels.data[i] + imgPixels.data[i + 1] + imgPixels.data[i + 2]) / 3;
 
-
imgPixels.data[i] = avg;
 
-
imgPixels.data[i + 1] = avg;
 
-
imgPixels.data[i + 2] = avg;
 
-
}
 
-
}
 
-
ctx.putImageData(imgPixels, 0, 0, 0, 0, imgPixels.width, imgPixels.height);
 
-
return canvas.toDataURL();
 
-
    }
 

Latest revision as of 22:44, 17 October 2014

iGEM BERLIN

Remote-controlled E. coli bacteria? That’s the aim of the interdisciplinary iGEM Team Berlin!

Magnetic E. coli?

The bacteria shall be optimized for the absorption and synthesis of iron in order to remote-control them magnetically. Small and efficient E. coli units with various areas of use shall be the result.

These cells are attractive as they offer new solutions in bio therapeutics, bio materials and bio process engineering. Furthermore, we want to use these cells as remote controlled bacteria that can be controlled by a real time computer interface.

Project

In October 2014, a team of Berlin students from a variety of backgrounds are participating for the first time in the international genetically engineered machines competition. For our project, the iGEM Berlin team has decided to make E. coli bacteria susceptible to magnetic fields. By knocking out the iron efflux transporter (FieF) and iron uptake suppressor (FUR), we want to increase the total iron level of the cytosol. By sequestering iron in a ferritin protein, iron crystals are formed and the cell is detoxified. In order to create ferromagnetic of super paramagnetic crystals, we will then use an intensive high through put media optimization to discover the best conditions for the formation of magnetic nanoparticles in E. coli. Furthermore, we will work with other metal binding proteins like metallothioneins and phytochelatin synthases in order to achieve nanoparticle synthesis in E. coli. Once we have discovered the best way to magnetize E. coli bacteria we will build and characterize a suitable BioBrick that can be used by any research lab or iGEM team in the world in order to remote control the movement of E. coli cells.

Team

Our team is made up of 17 undergraduates and postgraduates from a wide range of disciplines as well as our supervisors. The first iGEM Team Berlin 2014 is an assembly of highly motivated students from different universitys. Due to the multidisciplinarity our team is able to find different strategies to solve complex problems in synthetic biology. Hosted by Prof. Dr. Nediljko Budisa from the TU Berlin, our lab is located at the Müller-Breslau-Straße 10, 10623 Berlin. Come by for a visit (Monday 5 pm).

Safety

Our country uses a four-part „Safety Level“ rating system for laboratories. Level 4 is used for the most dangerous organisms. This is equivalent to the WHO system. The Safety Level of our lab is Level 1 (low risk).