Team:BIT/part.html

From 2014.igem.org

(Difference between revisions)
 
(44 intermediate revisions not shown)
Line 10: Line 10:
   
   
-
<script src="https://2014.igem.org/wiki/index.php?title=Team:BIT/jquery-1.3.2.min.js&amp;action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
+
<script src="https://2014.igem.org/wiki/index.php?title=Team:BIT/jquery-1.3.2.min.js&amp;action=raw&amp;ctype=text/javascript"type="text/javascript">
-
 
+
</script>
-
 
+
-
 
+
-
 
+
<style type="text/css">
<style type="text/css">
-
#main_content{ font-size:18px;}
+
#apDiv1 {
-
#footer1{ position:absolute;top:7400px;}
+
position:absolute;
-
#footer-box{ position:absolute;top:6200px;}
+
width:382px;
-
#left{ width:300px;height:300px; position:fixed; top:304px; left:180px;margin:10px 10px 0 0 ; z-index:2;}
+
height:115px;
-
#right{ width:704px;height:800px;position:absolute; top:10px; left:320px;margin:10px 0 0 0 ; }
+
z-index:1;
-
 
+
left: 442px;
-
*{margin:0;padding:0;list-style-type:none;}
+
top: 343px;
-
a,img{border:0;}
+
font-size: 36px;
-
 
+
color: #FFF;
-
a,a:hover{text-decoration: none;}
+
}
-
 
+
-
.table{ position:relative;}
+
-
.table img{ vertical-align:top;}
+
-
 
+
-
 
+
-
 
+
-
 
+
</style>
</style>
 +
<div id="header">
 +
<div style=" float:right;width:180px; height:100px; background:none;" ><a href="/Main_Page" title="Main Page"><img style="width: 150px; height: 60px;margin-top: 30px;margin-right: 25px;" src="https://static.igem.org/mediawiki/2014/e/e4/BIT_igem_logo.png"></a></div>
-
 
-
 
-
 
-
 
-
 
-
</head>
 
-
 
-
<body>
 
-
<div id="main_container"  >
 
-
 
-
 
-
<div id="container"  >
 
-
 
-
<img  src="https://static.igem.org/mediawiki/2014/a/ab/BIT_top.png" id="toTop" >
 
-
 
-
 
-
 
-
<div id="header">
 
-
<div style=" float:right;width:180px; height:100px; background:none;"><a href="/Main_Page" title="Main Page"><img style="width: 150px; height: 60px;margin-top: 30px;margin-right: 25px;" src="https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png"></a></div>
 
      
      
<ul class="nav">
<ul class="nav">
   
   
-
   <li > <a href="https://2014.igem.org/Team:BIT" class="label">home</a></li>
+
   <li> <a href="https://2014.igem.org/Team:BIT"class="label">home</a></li>
   
   
-
   <li> <a href="https://2014.igem.org/Team:BIT/project.html" class="label">project</a>
+
   <li > <a href="https://2014.igem.org/Team:BIT/project.html" class="label">project</a></li>
-
<!--<ul class="one" id="gallery">
+
-
      <li style="height:210px;">
+
-
   
+
-
        <div class="image"><a href="https://2014.igem.org/Team:BIT/abstract.html">abstract</a></div>
+
-
        <div class="image"><a href="https://2014.igem.org/Team:BIT/sensora.html">sensora</a></div>
+
-
        <div class="image"><a href="https://2014.igem.org/Team:BIT/sensorb.html">sensorb</a></div>
+
-
        <div class="image"><a href="https://2014.igem.org/Team:BIT/achievemen.html">achievement</a></div>
+
-
        <div class="image"><a href="https://2014.igem.org/Team:BIT/interlab.html">part</a></div>
+
-
      </li>
+
-
    </ul>-->
+
-
</li>
+
   <li  > <a href="https://2014.igem.org/Team:BIT/team.html" class="label">team</a></li>
   <li  > <a href="https://2014.igem.org/Team:BIT/team.html" class="label">team</a></li>
Line 81: Line 43:
   <li> <a href="https://2014.igem.org/Team:BIT/interlab.html" class="label">interlab</a></li>
   <li> <a href="https://2014.igem.org/Team:BIT/interlab.html" class="label">interlab</a></li>
   
   
-
   <li> <a href="https://2014.igem.org/Team:BIT/notebook.html" class="label">notebook</a></li>
+
   <li> <a href="https://2014.igem.org/Team:BIT/part.html" class="label">part</a></li>
    
    
   <li> <a href="https://2014.igem.org/Team:BIT/device.html" class="label">device</a></li>
   <li> <a href="https://2014.igem.org/Team:BIT/device.html" class="label">device</a></li>
Line 90: Line 52:
</div>
</div>
-
 
+
<div><span class="table"><img src="https://static.igem.org/mediawiki/2014/f/f3/BIT_blue.png" style="width:1000px;"></span>
 +
  <div id="apDiv1">Part Collection</div>
 +
  <table width="1024" border="1">
 +
    <tr>
 +
      <td width="55" rowspan="21">Amplifier</td>
 +
      <td width="103">BBa_K1407000</td>
 +
      <td width="844">RhlR CDS (no LVA tag) and terminator,RhlR/PAI2  inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by  adding PAI2,the part could be inducible and expresses RFP promoter.Before use  it,you'd better add a terminator to the part's tag.And add a promoter before  the RhlR as you want.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407001</td>
 +
      <td>RhlR/PAI2  inducible promoter with RBS (B0030) and RFP,terminator.By  adding terminator,we want to sure the RFP could be expressed accurately.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407002</td>
 +
      <td>RBS(B0034)+RhlR(LVA+)+RhlR/PAI2  inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by  adding PAI2, the part could be inducible and expresses RFP promoter.Before use  it,you'd better add a terminator to the part's tag.And add a promoter before  the RhlR as you want.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407003</td>
 +
      <td>constitutive  of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP.With the LasR  promoter existing,by adding PAI1,the part could be inducible and expresses RFP  promoter.Before use it,you'd better add a terminator to the part's tag.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407004</td>
 +
      <td>LasR/PAI1  Inducible promoter +RBS(B0034)+GFP and Terminator.With the LasR protein  existing,by adding PAI1,the part could be inducible and expresses GFP protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407005</td>
 +
      <td>LasR/PAI1  Inducible promoter +RBS(B0030)+RFP and Terminator.With the LasR protein  existing,by adding PAI1,the part could be inducible and expresses RFP protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407006</td>
 +
      <td>constitutive  production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP.With  the RhlR protein existing,by adding PAI2,the part could be inducible and  expresses RFP protein.Before using it,you'd better add a terminator to the  part's tag.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407007</td>
 +
      <td>RhlR  CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030)  and RFP,terminator.With the RhlR protein existing,by adding PAI2,the part could  be inducible and expresses RFP protein.Adding a promoter before the RhlR as you  want.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407008</td>
 +
      <td>constitutive  production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and  GFP,terminator.By adding PAI1,the part could be inducible and expresses GFP  protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407009</td>
 +
      <td>RhlR  protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and  RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP  protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407010</td>
 +
      <td>constitutive  production of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and  RFP,Terminator.By adding PAI1,the part could be inducible and expresses RFP  protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407011</td>
 +
      <td>constitutive  promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030)  and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP  protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407012</td>
 +
      <td>constitutive  promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS  (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and  expresses RFP protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407013</td>
 +
      <td>onstitutive  production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and  RFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2 inducible  promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part  could be inducible and expresses RFP protein.</td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407014</td>
 +
      <td><table border="1" cellspacing="0" cellpadding="0" width="100%">
 +
        <tr>
 +
          <td width="64%" valign="top"><p>constitutive production of LasR,LasR/PAI1    Inducible promoter with RBS(B0030)and RFP,terminator,constitutivepromoter,RhlR    protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and    RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducible and    expresses RFP protein.</p></td>
 +
        </tr>
 +
      </table></td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407015</td>
 +
      <td><table border="1" cellspacing="0" cellpadding="0" width="100%">
 +
        <tr>
 +
          <td width="64%" valign="top"><p>constitutive production of LasR,LasR/PAI1    Inducible promoter with RBS(B0034)and    GFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2    inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the    part could be inducible and expresses GFP protein;When PAI2 exsits,the part    could be inducible and expresses RFP protein.</p></td>
 +
        </tr>
 +
      </table></td>
 +
    </tr>
 +
    <tr>
 +
      <td>BBa_K1407016</td>
 +
      <td><table border="1" cellspacing="0" cellpadding="0" width="100%">
 +
        <tr>
 +
          <td width="64%" valign="top"><p>constitutive production of LasR,LasR/PAI1    Inducible promoter with RBS(B0034)and    GFP,terminator,constitutivepromoter,RhlR protein generator(LVA-) ,RhlR/PAI2    inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the    part could be inducible and expresses GFP protein;When PAI2 exsits,the part    could be inducible and expresses RFP protein.</p></td>
 +
        </tr>
 +
      </table></td>
 +
    </tr>
 +
 
 +
  </table>
 +
  <div>
 +
    <table width="1024" border="1">
 +
      <tr>
 +
        <td width="55" rowspan="21">Sensor</td>
 +
        <td width="103">BBa_K1407026</td>
 +
        <td width="844"><p>The recA promoter can response the  radiation.Radiation will start the sos response .The recA protein is the  important part of the sos response system.</p>
 +
Sequence  and Features</td>
 +
      </tr>
 +
      <tr>
 +
        <td>BBa_K1407027</td>
 +
        <td>Toggle  switch system with pRecA-LacI-pLac-LexA</td>
 +
      </tr>
 +
      <tr>
 +
        <td>BBa_K1407031</td>
 +
        <td>Lactose  or IPTG inducible RFP reporter</td>
 +
      </tr>
 +
      <tr>
 +
        <td>BBa_K1407099</td>
 +
        <td>The  recA promoter can response the radiation.Radiation will start the sos response  .The recA protein is the important part of the sos response system. From BL21</td>
 +
      </tr>
 +
      <tr>
 +
        <td>Ba_K1407036</td>
 +
        <td><p>Lux pL controlled LuxR with lux  pRautoinducingLuxI(lva tag)- AHL-GFP</p>
 +
amplifying  GFP reporter</td>
 +
      </tr>
 +
      <tr>
 +
       
 +
      </tr>
 +
    </table>
 +
    <p>&nbsp;</p>
 +
  </div>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
</div>
<div id="main_content" >
<div id="main_content" >
 +
  <center>
 +
    <div id="first" class="table">
 +
      <p style=" position:absolute; top:35px; left:430px; color:#FFFFFF; font-size:24px; letter-spacing: 2px;">Part Collection</p>
 +
      <div id="more1" style=" display:none;position:absolute; top:100px; width:420px; height:230px; background:rgba(255,255,255,0.7);"></div>
 +
    </div>
 +
  </center>
 +
</div>
 +
</body>
<center>
<center>
-
 
+
<div id="others" style="position:absolute; top:2182px; align=; left: 38px;"center";">
-
</center>     
+
<img style="width:1024px" src="https://static.igem.org/mediawiki/2014/4/42/BIT_line1.png">
-
 
+
<div id="logo">
-
<div id="others">
+
<img  src="https://static.igem.org/mediawiki/2014/6/6b/BIT_logo.png">
-
<center><img style="width:1024px"src="https://static.igem.org/mediawiki/2014/4/42/BIT_line1.png"></center>
+
</div>
-
<div id="logo"><img  src="https://static.igem.org/mediawiki/2014/6/6b/BIT_logo.png"></div>
+
     <div id="address">
     <div id="address">
-
     <center><p>Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081</p>
+
     <p>Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081</p>
         <p >E-mail: yifei0114@bit.edu.cn</p>
         <p >E-mail: yifei0114@bit.edu.cn</p>
-
         <p >Beijing Institute of Technology © 2014 Privacy Policy</p></center>
+
         <p >Beijing Institute of Technology © 2014 Privacy Policy</p>
     </div>
     </div>
</div>
</div>
-
 
+
</center>
-
 
+
-
 
+
-
</div>
+
-
</body>
+
-
 
+
<script src="https://2014.igem.org/wiki/index.php?title=Team:BIT/public.js&amp;action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
<script src="https://2014.igem.org/wiki/index.php?title=Team:BIT/public.js&amp;action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
-
 
-
 
-
 
 
</script>
</script>
</html>
</html>

Latest revision as of 03:20, 18 October 2014

<!DOCTYPE html> radiation measurement

Part Collection
Amplifier BBa_K1407000 RhlR CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by adding PAI2,the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.And add a promoter before the RhlR as you want.
BBa_K1407001 RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,terminator.By adding terminator,we want to sure the RFP could be expressed accurately.
BBa_K1407002 RBS(B0034)+RhlR(LVA+)+RhlR/PAI2 inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by adding PAI2, the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.And add a promoter before the RhlR as you want.
BBa_K1407003 constitutive of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP.With the LasR promoter existing,by adding PAI1,the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.
BBa_K1407004 LasR/PAI1 Inducible promoter +RBS(B0034)+GFP and Terminator.With the LasR protein existing,by adding PAI1,the part could be inducible and expresses GFP protein.
BBa_K1407005 LasR/PAI1 Inducible promoter +RBS(B0030)+RFP and Terminator.With the LasR protein existing,by adding PAI1,the part could be inducible and expresses RFP protein.
BBa_K1407006 constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP.With the RhlR protein existing,by adding PAI2,the part could be inducible and expresses RFP protein.Before using it,you'd better add a terminator to the part's tag.
BBa_K1407007 RhlR CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,terminator.With the RhlR protein existing,by adding PAI2,the part could be inducible and expresses RFP protein.Adding a promoter before the RhlR as you want.
BBa_K1407008 constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP,terminator.By adding PAI1,the part could be inducible and expresses GFP protein.
BBa_K1407009 RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP protein.
BBa_K1407010 constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI1,the part could be inducible and expresses RFP protein.
BBa_K1407011 constitutive promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP protein.
BBa_K1407012 constitutive promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP protein.
BBa_K1407013 onstitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and RFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducible and expresses RFP protein.
BBa_K1407014

constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and RFP,terminator,constitutivepromoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducible and expresses RFP protein.

BBa_K1407015

constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducible and expresses GFP protein;When PAI2 exsits,the part could be inducible and expresses RFP protein.

BBa_K1407016

constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutivepromoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducible and expresses GFP protein;When PAI2 exsits,the part could be inducible and expresses RFP protein.

Sensor BBa_K1407026

The recA promoter can response the radiation.Radiation will start the sos response .The recA protein is the important part of the sos response system.

Sequence and Features
BBa_K1407027 Toggle switch system with pRecA-LacI-pLac-LexA
BBa_K1407031 Lactose or IPTG inducible RFP reporter
BBa_K1407099 The recA promoter can response the radiation.Radiation will start the sos response .The recA protein is the important part of the sos response system. From BL21
Ba_K1407036

Lux pL controlled LuxR with lux pRautoinducingLuxI(lva tag)- AHL-GFP

amplifying GFP reporter

 

 

 

Part Collection

Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081

E-mail: yifei0114@bit.edu.cn

Beijing Institute of Technology © 2014 Privacy Policy