Team:BIOSINT Mexico/Chassis

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In biotechnology plants are mainly used for premeditation, for the obtainment of industrial products and to generate energy. For these reasons plant manipulation is a focus area in biological engineering. Unfortunately there is not a wide variety of plants projects in iGEM, one reason it's the lack of information in the registry of these organisms.
In biotechnology plants are mainly used for premeditation, for the obtainment of industrial products and to generate energy. For these reasons plant manipulation is a focus area in biological engineering. Unfortunately there is not a wide variety of plants projects in iGEM, one reason it's the lack of information in the registry of these organisms.
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''Arabidopsis thaliana'' is a model organism that was previously described as chassis. In iGEM 2010, Harvard team use this plant to test their designed vectors for ''Agrobacterium tumefaciens''; in 2012 the Kyoto team work with ''A. thaliana'' as model for the induction of flower formation by putting ''E. coli'' in the leaves;
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''Arabidopsis thaliana'' is a model organism that was previously described as chassis. In iGEM 2010, Harvard team use this plant to test their designed vectors for ''Agrobacterium tumefaciens''; in 2012 the Kyoto team work with ''A. thaliana'' as model for the induction of flower formation by putting ''E. coli'' in the leaves.
Compared with other organisms used as models, the proportion of ''A. thaliana'' proteins have related counterparts in Eukaryota genomes, these varies by a factor of 2 to 3, depending on the functional category. Only 8-23% of ''A. thaliana'' proteins involved in transcription have related genes in other Eukaryota genomes, reflecting the independent evolution of many plant transcription factors.
Compared with other organisms used as models, the proportion of ''A. thaliana'' proteins have related counterparts in Eukaryota genomes, these varies by a factor of 2 to 3, depending on the functional category. Only 8-23% of ''A. thaliana'' proteins involved in transcription have related genes in other Eukaryota genomes, reflecting the independent evolution of many plant transcription factors.

Revision as of 06:19, 17 October 2014

Chassis.png

Arabidopsis - standar chassis in iGEM

Description

In biotechnology plants are mainly used for premeditation, for the obtainment of industrial products and to generate energy. For these reasons plant manipulation is a focus area in biological engineering. Unfortunately there is not a wide variety of plants projects in iGEM, one reason it's the lack of information in the registry of these organisms.

Arabidopsis thaliana is a model organism that was previously described as chassis. In iGEM 2010, Harvard team use this plant to test their designed vectors for Agrobacterium tumefaciens; in 2012 the Kyoto team work with A. thaliana as model for the induction of flower formation by putting E. coli in the leaves.

Compared with other organisms used as models, the proportion of A. thaliana proteins have related counterparts in Eukaryota genomes, these varies by a factor of 2 to 3, depending on the functional category. Only 8-23% of A. thaliana proteins involved in transcription have related genes in other Eukaryota genomes, reflecting the independent evolution of many plant transcription factors.

In contrast, 48-60% of genes involved in protein synthesis have counterpart in other eukaryota genomes, reflecting highly conserved gene functions. The relatively high proportion of matches between A. thaliana and bacterial proteins in the categories “metabolism” and “energy” reflects, both, the acquisition of bacterial genes from the ancestor of the plastid and high conservation of sequences across all species.

Advantages

Disadvantages

How to use Arabidopsis thaliana?

Successful germination and plant growth requires appropriate soil moisture, nutrient levels, light intensity, humidity, and temperature. If any of these are compromised, A. thaliana will respond by flowering early and dying prematurely and producing little leaf mass for experiments. The plants can also be stressed by overcrowding, fungus infestation, or insect infestation. All these factors must be conscientiously monitored to ensure reliable data obtained from experiments using these plants. Maintenance of soil moisture is imperative for successful seed germination.

The optimal temperature for plant growth is 25°C, with lower temperatures being allowable; higher temperatures can be very detrimental, particularly in the first two weeks of growth.

For transformation we used the floral dip method described in this protocol: (Sushanta, 2013).


Modeling


Results


References