Team:Aix-Marseille/Parts

From 2014.igem.org

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          <h2 class="subtitle">Part BBa_K1349001</h3>
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            <h2 class="subtitle">Part BBa_K1349001</h3>
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          <p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1349001" target="_blank">Link to registry</a></p>
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            <p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1349001" target="_blank">Link to registry</a></p>
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            <h3>Name</h3>
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              <h3><i>relA</i></h3>
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            <p><i>relA</i></p>
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             <h3>Description</h3>
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             <h4>Description</h4>
             <p>The <i>relA</i> gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from <i>E. coli</i> W3110 was mutated by site-directed mutagenesis to remove the PstI site.</p>
             <p>The <i>relA</i> gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from <i>E. coli</i> W3110 was mutated by site-directed mutagenesis to remove the PstI site.</p>
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           <div class="project-subsection">
           <div class="project-subsection">

Revision as of 12:00, 16 October 2014

Our parts

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Part BBa_K1349001

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relA

Description

The relA gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from E. coli W3110 was mutated by site-directed mutagenesis to remove the PstI site.

Part BBa_K1349002

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Name

CusR box

Description

The CusR box allow the binding of the CusR regulator. In E. coli, the CusR/CusS two-component system induces expression of genes involved in metal efflux under condition of elevated Cu concentration. Phosphorylated CusR specifically bind to CusR-dependent promoters.

Part BBa_K1349004

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Name

mesh1

Description

Mesh1 is a SpoT orthologs in metazoa. It catalyzes hydrolysis of ppGpp (guanosine 3'-diphosphate 5-' diphosphate).

Other parts

Part BBa_K1349000 - serA_troncated(1-->1008nt)

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Part BBa_K1349003 - PcusC

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Part BBa_K1349005 - (SH3)x1

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Part BBa_K1349006 - cheA

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Part BBa_K1349007 - CusR-L-LZA

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Part BBa_K1349008 - (SH3pep)-L-LZA

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