Team:Aix-Marseille/Parts

From 2014.igem.org

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-
<html>
 
   <div class="container">
   <div class="container">
     <h1 class="project-title">Our parts</h1>
     <h1 class="project-title">Our parts</h1>
-
    <br><br><br>
 
-
    <i>The list will be updated each time we'll create or modify a part</i>
 
-
    <br><br><br>
 
-
    <div class="project-section">
 
-
      <span class="project-tag" id="details"></span>
 
-
      <h1>Part BBa_K1349000</h1>
 
      
      
-
       <div class="project-subsection">
+
    <div class="row" style="position:relative;">
-
         <span class="project-tag" id="serine_part"></span>
+
       <!-- Content -->
-
        <h3 class="subtitle">Short description</h3>
+
      <!-- ******* -->
-
        <p>SerA_mut</p>
+
      <div class="col-md-9 project">
-
      </div>
+
        <br><br><br>
 +
        <i>The list will be updated each time we'll create or modify a part</i>
 +
        <br><br><br>
 +
 
 +
        <div class="project-section">
 +
          <span class="project-tag" id="details"></span>
 +
          <h1>Part BBa_K1349000</h1>
 +
          
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Short description</h3>
 +
            <p>SerA_mut</p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">DNA Sequence</h3>
 +
            <p>ATGGCAAAGGTATCGCTGGAGAAAGACAAGATTAAGTTTCTGCTGGTAGAAGGCGTGCACCAAAAGGCGC TGGAAAGCCTTCGTGCAGCTGGTTACACCAACATCGAATTTCACAAAGGCGCGCTGGATGATGAACAATT AAAAGAATCCATCCGCGATGCCCACTTCATCGGCCTGCGATCCCGTACCCATCTGACTGAAGACGTGATC AACGCCGCAGAAAAACTGGTCGCTATTGGCTGTTTCTGTATCGGAACAAACCAGGTTGATCTGGATGCGG CGGCAAAGCGCGGGATCCCGGTATTTAACGCACCGTTCTCAAATACGCGCTCTGTTGCGGAGCTGGTGAT TGGCGAACTGCTGCTGCTATTGCGCGGCGTGCCGGAAGCCAATGCTAAAGCGCACCGTGGCGTGTGGAAC AAACTGGCGGCGGGTTCTTTTGAAGCGCGCGGCAAAAAGCTGGGTATCATCGGCTACGGTCATATTGGTA CGCAATTGGGCATTCTGGCTGAATCGCTGGGAATGTATGTTTACTTTTATGATATTGAAAATAAACTGCC GCTGGGCAACGCCACTCAGGTACAGCATCTTTCTGACCTGCTGAATATGAGCGATGTGGTGAcgctGCAT GTACCAGAGAATCCGTCCACCAAAAATATGATGGGCGCGAAAGAAATTTCACTAATGAAGCCCGGCTCGC TGCTGATTAATGCTTCGCGCGGTACTGTGGTGGATATTCCGGCGCTGTGTGATGCGCTGGCGAGCAAACA TCTGGCGGGGGCGGCAATCGACGTATTCCCGACGGAACCGGCGACCAATAGCGATCCATTTACCTCTCCG CTGTGTGAATTCGACAACGTCCTTCTGACGCCACACATTGGCGGTTCGACTCAGGAAGCGCAGGAGAATA TCGGCCTGGAAGTTGCGGGTAAATTGATCAAGTATTCTGACAATGGCTCAACGCTCTCTGCGGTGAACTT CCCGGAAGTCTCGCTGCCACTGCACGGT</p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Logic and explanation for part and construction</h3>
 +
            <p>This construct encodes a truncated version of E. coli W3110 SerA ( D-3-phosphoglycerate dehydrogenase), missing the last 75 aa of the full sequence, and without stop codon. SerA is requiered for serine biosynthesis. Following the work of Peters-Wendisch et al. 2005, this mutated version of the enzyme is expected to be no-longer inhibited by Serine, and to allow the production of a high serine concentration. </p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Purpose</h3>
 +
            <p>This part is designed to allow the synthesis of serine in E. coli without the normal feedback inhibition, thus permitting the accumulation of high concentrations.</p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Origin and method of construction</h3>
 +
            <p>The part was obtained by PCR from E.coli strain W3110 and SLIC assembly. The construction removed the EcoR1 restriction site in the gene by the silent mutations of a GAA codon to a GAG codon.</p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Propreties expected and validation</h3>
 +
            <p>We designed this sequence that in an E.coli mutant strain unable to catabolise serine correctly and without a serine import system, production of this coding sequence will lead to serine secretion into the growth media. The sequence does not contain a stop codon so it should be incorporated into composite bricks for testing synthesis and activity.</p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Current backbone</h3>
 +
            <p>pSB1C3</p>
 +
          </div>
 +
       
 +
          <div class="project-subsection">
 +
            <span class="project-tag" id="serine_part"></span>
 +
            <h3 class="subtitle">Sequenced clone</h3>
 +
            <p>ok SerA Cm1</p>
 +
          </div>
 +
       
 +
        </div>
      
      
-
       <div class="project-subsection">
+
      </div> <!-- /Content -->
-
         <span class="project-tag" id="serine_part"></span>
+
     
-
        <h3 class="subtitle">DNA Sequence</h3>
+
     
-
        <p>ATGGCAAAGGTATCGCTGGAGAAAGACAAGATTAAGTTTCTGCTGGTAGAAGGCGTGCACCAAAAGGCGC TGGAAAGCCTTCGTGCAGCTGGTTACACCAACATCGAATTTCACAAAGGCGCGCTGGATGATGAACAATT AAAAGAATCCATCCGCGATGCCCACTTCATCGGCCTGCGATCCCGTACCCATCTGACTGAAGACGTGATC AACGCCGCAGAAAAACTGGTCGCTATTGGCTGTTTCTGTATCGGAACAAACCAGGTTGATCTGGATGCGG CGGCAAAGCGCGGGATCCCGGTATTTAACGCACCGTTCTCAAATACGCGCTCTGTTGCGGAGCTGGTGAT TGGCGAACTGCTGCTGCTATTGCGCGGCGTGCCGGAAGCCAATGCTAAAGCGCACCGTGGCGTGTGGAAC AAACTGGCGGCGGGTTCTTTTGAAGCGCGCGGCAAAAAGCTGGGTATCATCGGCTACGGTCATATTGGTA CGCAATTGGGCATTCTGGCTGAATCGCTGGGAATGTATGTTTACTTTTATGATATTGAAAATAAACTGCC GCTGGGCAACGCCACTCAGGTACAGCATCTTTCTGACCTGCTGAATATGAGCGATGTGGTGAcgctGCAT GTACCAGAGAATCCGTCCACCAAAAATATGATGGGCGCGAAAGAAATTTCACTAATGAAGCCCGGCTCGC TGCTGATTAATGCTTCGCGCGGTACTGTGGTGGATATTCCGGCGCTGTGTGATGCGCTGGCGAGCAAACA TCTGGCGGGGGCGGCAATCGACGTATTCCCGACGGAACCGGCGACCAATAGCGATCCATTTACCTCTCCG CTGTGTGAATTCGACAACGTCCTTCTGACGCCACACATTGGCGGTTCGACTCAGGAAGCGCAGGAGAATA TCGGCCTGGAAGTTGCGGGTAAATTGATCAAGTATTCTGACAATGGCTCAACGCTCTCTGCGGTGAACTT CCCGGAAGTCTCGCTGCCACTGCACGGT</p>
+
      <!-- Table of Contents -->
 +
      <!-- ***************** -->
 +
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 +
         <div id="stop_top" style="height:50px"></div>
 +
        <div id="sidebar-project">
 +
          <ul class="nav">
 +
            <li class="active">
 +
              <a data-scroll href="#intro">Introduction</a>
 +
            </li>
 +
            <li>
 +
              <a data-scroll href="#mod_sys">Model system</a>
 +
            </li>
 +
            <li>
 +
              <a data-scroll href="#simplify">Simplifying assumptions</a>
 +
            </li>
 +
            <li>
 +
              <a data-scroll href="#diff_equa">Differential equations</a>
 +
            </li>
 +
            <li>
 +
              <a data-scroll href="#init_val">Initial values</a>
 +
            </li>
 +
            <li>
 +
              <a data-scroll href="#resolv_sys">Resolution of the system</a>
 +
              <ul class="nav">
 +
                <li><a data-scroll href="#case1">First case</a></li>
 +
                <li><a data-scroll href="#case2">Second case</a></li>
 +
                <li><a data-scroll href="#case3">Third case</a></li>
 +
                <li><a data-scroll href="#case4">Fourth case</a></li>
 +
              </ul>
 +
            </li>
 +
          </ul>
 +
        </div>
       </div>
       </div>
-
   
 
-
      <div class="project-subsection">
 
-
        <span class="project-tag" id="serine_part"></span>
 
-
        <h3 class="subtitle">Logic and explanation for part and construction</h3>
 
-
        <p>This construct encodes a truncated version of E. coli W3110 SerA ( D-3-phosphoglycerate dehydrogenase), missing the last 75 aa of the full sequence, and without stop codon. SerA is requiered for serine biosynthesis. Following the work of Peters-Wendisch et al. 2005, this mutated version of the enzyme is expected to be no-longer inhibited by Serine, and to allow the production of a high serine concentration. </p>
 
-
      </div>
 
-
   
 
-
      <div class="project-subsection">
 
-
        <span class="project-tag" id="serine_part"></span>
 
-
        <h3 class="subtitle">Purpose</h3>
 
-
        <p>This part is designed to allow the synthesis of serine in E. coli without the normal feedback inhibition, thus permitting the accumulation of high concentrations.</p>
 
-
      </div>
 
-
   
 
-
      <div class="project-subsection">
 
-
        <span class="project-tag" id="serine_part"></span>
 
-
        <h3 class="subtitle">Origin and method of construction</h3>
 
-
        <p>The part was obtained by PCR from E.coli strain W3110 and SLIC assembly. The construction removed the EcoR1 restriction site in the gene by the silent mutations of a GAA codon to a GAG codon.</p>
 
-
      </div>
 
-
   
 
-
      <div class="project-subsection">
 
-
        <span class="project-tag" id="serine_part"></span>
 
-
        <h3 class="subtitle">Propreties expected and validation</h3>
 
-
        <p>We designed this sequence that in an E.coli mutant strain unable to catabolise serine correctly and without a serine import system, production of this coding sequence will lead to serine secretion into the growth media. The sequence does not contain a stop codon so it should be incorporated into composite bricks for testing synthesis and activity.</p>
 
-
      </div>
 
-
   
 
-
      <div class="project-subsection">
 
-
        <span class="project-tag" id="serine_part"></span>
 
-
        <h3 class="subtitle">Current backbone</h3>
 
-
        <p>pSB1C3</p>
 
-
      </div>
 
-
   
 
-
      <div class="project-subsection">
 
-
        <span class="project-tag" id="serine_part"></span>
 
-
        <h3 class="subtitle">Sequenced clone</h3>
 
-
        <p>ok SerA Cm1</p>
 
-
      </div>
 
-
   
 
     </div>
     </div>
 +
    <div id="stop_bot"></div>
   </div>
   </div>
</html>
</html>
{{Team:Aix-Marseille/footer}}
{{Team:Aix-Marseille/footer}}

Revision as of 21:02, 15 October 2014

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Contents

Our parts

       



       The list will be updated each time we'll create or modify a part

Part BBa_K1349000

Short description

SerA_mut

DNA Sequence

ATGGCAAAGGTATCGCTGGAGAAAGACAAGATTAAGTTTCTGCTGGTAGAAGGCGTGCACCAAAAGGCGC TGGAAAGCCTTCGTGCAGCTGGTTACACCAACATCGAATTTCACAAAGGCGCGCTGGATGATGAACAATT AAAAGAATCCATCCGCGATGCCCACTTCATCGGCCTGCGATCCCGTACCCATCTGACTGAAGACGTGATC AACGCCGCAGAAAAACTGGTCGCTATTGGCTGTTTCTGTATCGGAACAAACCAGGTTGATCTGGATGCGG CGGCAAAGCGCGGGATCCCGGTATTTAACGCACCGTTCTCAAATACGCGCTCTGTTGCGGAGCTGGTGAT TGGCGAACTGCTGCTGCTATTGCGCGGCGTGCCGGAAGCCAATGCTAAAGCGCACCGTGGCGTGTGGAAC AAACTGGCGGCGGGTTCTTTTGAAGCGCGCGGCAAAAAGCTGGGTATCATCGGCTACGGTCATATTGGTA CGCAATTGGGCATTCTGGCTGAATCGCTGGGAATGTATGTTTACTTTTATGATATTGAAAATAAACTGCC GCTGGGCAACGCCACTCAGGTACAGCATCTTTCTGACCTGCTGAATATGAGCGATGTGGTGAcgctGCAT GTACCAGAGAATCCGTCCACCAAAAATATGATGGGCGCGAAAGAAATTTCACTAATGAAGCCCGGCTCGC TGCTGATTAATGCTTCGCGCGGTACTGTGGTGGATATTCCGGCGCTGTGTGATGCGCTGGCGAGCAAACA TCTGGCGGGGGCGGCAATCGACGTATTCCCGACGGAACCGGCGACCAATAGCGATCCATTTACCTCTCCG CTGTGTGAATTCGACAACGTCCTTCTGACGCCACACATTGGCGGTTCGACTCAGGAAGCGCAGGAGAATA TCGGCCTGGAAGTTGCGGGTAAATTGATCAAGTATTCTGACAATGGCTCAACGCTCTCTGCGGTGAACTT CCCGGAAGTCTCGCTGCCACTGCACGGT

Logic and explanation for part and construction

This construct encodes a truncated version of E. coli W3110 SerA ( D-3-phosphoglycerate dehydrogenase), missing the last 75 aa of the full sequence, and without stop codon. SerA is requiered for serine biosynthesis. Following the work of Peters-Wendisch et al. 2005, this mutated version of the enzyme is expected to be no-longer inhibited by Serine, and to allow the production of a high serine concentration.

Purpose

This part is designed to allow the synthesis of serine in E. coli without the normal feedback inhibition, thus permitting the accumulation of high concentrations.

Origin and method of construction

The part was obtained by PCR from E.coli strain W3110 and SLIC assembly. The construction removed the EcoR1 restriction site in the gene by the silent mutations of a GAA codon to a GAG codon.

Propreties expected and validation

We designed this sequence that in an E.coli mutant strain unable to catabolise serine correctly and without a serine import system, production of this coding sequence will lead to serine secretion into the growth media. The sequence does not contain a stop codon so it should be incorporated into composite bricks for testing synthesis and activity.

Current backbone

pSB1C3

Sequenced clone

ok SerA Cm1


</html>

Contact us

Email : equipe.igem.amu@gmail.com

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Retrieved from "http://2014.igem.org/Team:Aix-Marseille/Parts"