Team:Aberdeen Scotland/Parts/ 6007

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<img src="http://parts.igem.org/wiki/images/d/dd/Restriction_digest_table.png">
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<img src="http://parts.igem.org/wiki/images/0/06/Restriction_digest_on_BBa_K346007.png">
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<font size="2">Fig.1&nbsp;&nbsp;&nbsp;&nbsp;Gel electrophoresis on standard restriction digests of BBa_K346007
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<img src="http://parts.igem.org/wiki/images/d/dd/Restriction_digest_table.jpg">
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<font size="2">Fig.2&nbsp;&nbsp;&nbsp;&nbsp;Fragment sizes resulted from standard restriction digests on BBa_K346007.
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<p>Antigen 43 (Ag43), the product of the </i>flu</i> gene, is a cell-surface autotransporter protein found in <i>Escherichia coli</i>. It is expressed at about 50,
<p>Antigen 43 (Ag43), the product of the </i>flu</i> gene, is a cell-surface autotransporter protein found in <i>Escherichia coli</i>. It is expressed at about 50,
000 copies/cell and is initially synthesised as a precursor of 1039 amino acids. Upon removal of the signal peptide, the protein is transported to the cell surface
000 copies/cell and is initially synthesised as a precursor of 1039 amino acids. Upon removal of the signal peptide, the protein is transported to the cell surface

Revision as of 21:38, 17 October 2014

Team:Aberdeen Scotland/Parts - 2014.ogem.org



Background to Parts Design





Fig.1    Gel electrophoresis on standard restriction digests of BBa_K346007



Fig.2    Fragment sizes resulted from standard restriction digests on BBa_K346007.


Antigen 43 (Ag43), the product of the flu gene, is a cell-surface autotransporter protein found in Escherichia coli. It is expressed at about 50, 000 copies/cell and is initially synthesised as a precursor of 1039 amino acids. Upon removal of the signal peptide, the protein is transported to the cell surface and is composed of an α subunit (499 amino acids) at the N-terminus and a β subunit (488 amino acids) at the C-terminus. Ag43 is mainly known to induce cell-to-cell aggregation and be involved in biofilm formation. However, as the necessary information required for auto transportation resides in the protein itself, the main of our project was to use it as a platform for displaying specific peptides on the surface of E. coli.

Ag43