Team:Aberdeen Scotland/Parts/ 2006

From 2014.igem.org

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<p>Antigen 43 (Ag43), the product of the </i>flu</i> gene, is a cell-surface autotransporter protein found in <i>Escherichia coli</i>. It is expressed at about 50,
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000 copies/cell and is initially synthesised as a precursor of 1039 amino acids. Upon removal of the signal peptide, the protein is transported to the cell surface
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and is composed of an α subunit (499 amino acids) at the N-terminus and a β subunit (488 amino acids) at the C-terminus. Ag43 is mainly known to induce cell-to-cell
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aggregation and be involved in biofilm formation. However, as the necessary information required for auto transportation resides in the protein itself, the main of
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our project was to use it as a platform for displaying specific peptides on the surface of <i>E. coli</i>.</p>
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<img src="http://2014.igem.org/wiki/images/2/2e/Ag43.jpg" alt="Ag43">
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Revision as of 20:37, 17 October 2014

Team:Aberdeen Scotland/Parts - 2014.ogem.org



Background to Parts Design



Figure 1; a Xba1 + HindIII restriction digest screen of recombinants. The recombinant which went on to become K1352006 is in lane 5. The “L” lane is DNA marker (ladder). The arrows are to highlight the distance travelled by the HindIII-negative recombinants.


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Fig.1    Graphic representation of Ag43 autotransporter structure and process of autotransportation.