Team:Aberdeen Scotland/Modeling/QS

From 2014.igem.org

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<p>Diffusion Equation</p>
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<p>Our goal with this model was to analyse our system in detail so that we learn more about its spacial features. Namely we wanted to see if co-localised cells will be able to distinguish each other from far away cells through quorum sensing and if so under what conditions. This was essential to our design as initially we envisioned that cells will communicate and create an AND-gate type of response in presence of according stimuli.</p>
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<p>Since Quorum Sensing(QS) occurs due to diffusion of molecules in the medium, we naturally decided to employ the diffusion equation.</p>
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<p>Diffusion Equation (more precisely Fick's 2nd Law)<sup>1</sup></p>
$$\frac{\partial{C}}{\partial{t}}=D\nabla^2{C}$$
$$\frac{\partial{C}}{\partial{t}}=D\nabla^2{C}$$
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<p>However, in our system there is a dedicated Sender and Receiver cells, which is different from natural QS. Sender cells can only produce and diffuse AHLs into the medium without the ability to react to it and Receiver cells can only react to AHLs without the ability to produce it. This arrangement made our system particularly interesting as we will find further down.</p>
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<ul>
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<li><h3>Initial Assumptions</h3>
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<ul>
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<li>Constant Production of AHL from Sender cells</li>
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<li>Receivers react to the AHL concentration around them</li>
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<li><i>E. Coli</i> cell-shape symmetry is taken into account</li>
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</ul>
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<img src="https://static.igem.org/mediawiki/2014/4/4e/Qs1.png">
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<h3>References</h3>
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<p><a href="http://www.uni-leipzig.de/diffusion/journal/pdf/volume2/diff_fund_2(2005)1.pdf">[1] Jean Philibert, One and a Half Century of Diffusion: Fick, Einstein,
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before and beyond, Diffusion Fundamentals 2, 2005 1.1–1.10</a></p>
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Revision as of 16:17, 17 October 2014

Team:Aberdeen Scotland/Modelling - 2014.ogem.org



Quorum Sensing Model


Our goal with this model was to analyse our system in detail so that we learn more about its spacial features. Namely we wanted to see if co-localised cells will be able to distinguish each other from far away cells through quorum sensing and if so under what conditions. This was essential to our design as initially we envisioned that cells will communicate and create an AND-gate type of response in presence of according stimuli.

Since Quorum Sensing(QS) occurs due to diffusion of molecules in the medium, we naturally decided to employ the diffusion equation.

Diffusion Equation (more precisely Fick's 2nd Law)1

$$\frac{\partial{C}}{\partial{t}}=D\nabla^2{C}$$

However, in our system there is a dedicated Sender and Receiver cells, which is different from natural QS. Sender cells can only produce and diffuse AHLs into the medium without the ability to react to it and Receiver cells can only react to AHLs without the ability to produce it. This arrangement made our system particularly interesting as we will find further down.

  • Initial Assumptions

    • Constant Production of AHL from Sender cells
    • Receivers react to the AHL concentration around them
    • E. Coli cell-shape symmetry is taken into account

References

[1] Jean Philibert, One and a Half Century of Diffusion: Fick, Einstein, before and beyond, Diffusion Fundamentals 2, 2005 1.1–1.10