Team:Aberdeen Scotland/Modeling/GFP

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<p>Hi, there. We are a team of Aberdeen Uni undergrads trying to do our part in the fight against Sleeping Sickness. There's six of us - 5 biologists and 1 physicist.
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<p>The idea behind this model was to ensure our Receiver->Sender design is viable and Quorum Sensing will trigger GFP production. It is a simple model that explores
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We are very excited to be able to take part in iGEM and we would like to take you on a tour around our project.</p>
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the production of GFP protein in the Receiver cell due to AHL-activated LuxR.</p>
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<p>We have worked all summer towards what we hope would turn out to be some peace of mind for a lot of people. The goal is to develop a novel method for diagnosing
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Trypanosomiasis. A simpler, cheaper alternative to current methods that would be more versatile in developing countries and their remote regions. We wish to create a
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<p>Since the Receivers do not produce AHL, the surrounding AHL concentration is not affected by the Receiver itself. Thus self-stimulation is out of the picture and
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test that would be portable, endure harsh environmental conditions and most importantly be sensitive to the early stages of the disease.</p>
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we can assume the amount of AHL is solely due to the "background".</p>
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<p>This would give a lot of unsuspecting sufferers the chance to get diagnosed early. This way they can get cured quickly, before the disease reaches its later
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stages, when it is virtually incurable.</p>
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<p>We use the following equation to model how GFP production is triggered:</p>
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$$\frac{d[AHL-LuxR]}{dt} = k_{+}[AHL][LuxR]-k_{-}[AHL-LuxR]$$
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Revision as of 18:47, 17 October 2014

Team:Aberdeen Scotland/Modelling - 2014.ogem.org



GFP Response Model


The idea behind this model was to ensure our Receiver->Sender design is viable and Quorum Sensing will trigger GFP production. It is a simple model that explores the production of GFP protein in the Receiver cell due to AHL-activated LuxR.

Since the Receivers do not produce AHL, the surrounding AHL concentration is not affected by the Receiver itself. Thus self-stimulation is out of the picture and we can assume the amount of AHL is solely due to the "background".

We use the following equation to model how GFP production is triggered:

$$\frac{d[AHL-LuxR]}{dt} = k_{+}[AHL][LuxR]-k_{-}[AHL-LuxR]$$


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