Team:Aalto-Helsinki

From 2014.igem.org

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Our aim is to build active synthetic biology community in Finland, to learn and share what biotech entrepreneurship takes and to find an open way to build business on synthetic biology.
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Figuring out how to combine business with science.
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<small>A Synthetic Biology Startup with a Three-Channel Gene Switch</small></h2>
<small>A Synthetic Biology Startup with a Three-Channel Gene Switch</small></h2>
 +
<h3>Project Description</h3>
<p>
<p>
We have engineered a three-channel switch that is controlled with the intensity of blue light. By utilizing the mechanisms of the lambda repressor, we are able to switch between the expressions of three different genes with a short delay. This kind of mechanism provides a nearly real-time control over genes, which could provide advantages in variety of industrial bioprocesses.
We have engineered a three-channel switch that is controlled with the intensity of blue light. By utilizing the mechanisms of the lambda repressor, we are able to switch between the expressions of three different genes with a short delay. This kind of mechanism provides a nearly real-time control over genes, which could provide advantages in variety of industrial bioprocesses.
</p>
</p>
<p>
<p>
-
Inspired by the principles of open source software, we introduce an Open Sequence business model implemented around our switch. We want to encourage future companies to create Open Sequence based solutions and to empower customers to participate in the product development. Open Sequence model’s transparency as well as lower degree of protection raises trust and continuity and benefits larger community.
+
Inspired by the principles of open source software, we introduce an Open Source business model implemented around our switch. We want to encourage future companies to create Open Source based solutions and to empower customers to participate in the product development. Open Source model’s transparency as well as lower degree of protection raises trust and continuity and benefits larger community.
</p>
</p>
<p>
<p>
We want to share our experiences and insights in biotech entrepreneurship and address the difficulties that students and newcomers may face in the early stages of building a synthetic biology startup.
We want to share our experiences and insights in biotech entrepreneurship and address the difficulties that students and newcomers may face in the early stages of building a synthetic biology startup.
</p>
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            <a href="http://youtu.be/tlc7MPY9SE8"><img src="https://static.igem.org/mediawiki/2014/7/79/Aaltohelsinki_pitc_capture.png" class="img-responsive smallerimg"></img></a>
 +
            <p class="kuvateksti">
 +
                Our pitch video on August 6th, Summer of Startups Demo Day.
 +
            </p>
 +
        </div>
 +
    </div>
<img src="https://static.igem.org/mediawiki/2014/7/7e/Aalto_Helsinki_Logot_Oma.png" class="img-responsive center-block omalogot">
<img src="https://static.igem.org/mediawiki/2014/7/7e/Aalto_Helsinki_Logot_Oma.png" class="img-responsive center-block omalogot">
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 +
<h2>The Story of Aalto-Helsinki Bioworks</h2>
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<div class="row">
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<div class="col-md-6 text-col-left">
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<p>
 +
We are Aalto-Helsinki Bioworks, the first-ever Finnish iGEM Team and one of the four teams in the new Entrepreneurship Track. Our <a href="https://2014.igem.org/Team:Aalto-Helsinki/Team">team</a> consists of nine students from Aalto University and the University of Helsinki and we are combining our interdisciplinary forces to develop something new and fascinating. We want to put Finland on the map of synthetic biology and improve undergraduate research opportunities in our universities.
 +
</p>
 +
<p>
 +
We have <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research">designed</a> a three-channel gene switch that would make it possible to control three user-defined genes with blue light intensity. We are using <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research#yf1-title">YF1</a> as our light receptor protein and the signal is mediated to lambda repressor protein (CI) production via phosphorylation pathway. The concentration of the CI protein defines the gene that should be active at a time. The secret behind this function is our modified version of the <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research#lambda-title">lambda repressor</a> mechanism.
 +
</p>
 +
<p>
 +
We constructed a mathematical <a href="https://2014.igem.org/Team:Aalto-Helsinki/Modeling">model</a> that simulates the interactions of the molecules and function of the gene switch we designed. This revealed interesting phenomena in the dynamics of our system which helps us to better understand the capabilities and limitations of the gene switch. We also made an <a href="http://igem-qsf.github.io/SimCircus/WebUI/">interactive simulation</a> available to everybody to best illustrate how our idea actually works.
 +
</p>
 +
<p>
 +
To control the amount of blue light on our cell cultures, we created the <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research#ledrig-title">LED rig</a> device. This enabled us to perform diverse experiments in order to characterize our light response element. These <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research#Results">results</a> show that our light response element is able to regulate the downstream gene expression precisely. In addition, the response time is in a ten-minute-scale, which enables constant and nearly real-time control over bacterial cultures. In addition, we hypothesize that this system is also applicable to bioreactors, which would enable higher level of control in industrial bioprocesses. However, our fully functional prototype is still under ongoing development.
 +
</p>
 +
<p>
 +
We have developed an open approach to <a href="https://2014.igem.org/Team:Aalto-Helsinki/Business">biotech business</a> inspired by the business models for open source software. Following the Open Source philosophy, we will distribute open Aalto-Helsinki Bioworks technologies for free to everybody - as long as they share the improvements made on the technology. Therefore, we empower our users to participate in product development. Our <a href="https://2014.igem.org/Team:Aalto-Helsinki/Business#canvas-title">business model</a> is based on hardware and wide range of services offered to our customers.
 +
</p>
 +
<p>
 +
During the summer we participated in Aalto Entrepreneurship Society’s <a href="https://2014.igem.org/Team:Aalto-Helsinki/Business#Sos">Summer of Startups 2014</a> incubator programme to learn the essential entrepreneurial skills, such as pitching, investor relations, customer-driven development and <a href="https://2014.igem.org/Team:Aalto-Helsinki/Business#pitch-title">explaining</a> the science for general audience. See our Demo Day video for details.
 +
</p>
 +
<p>
 +
iGEM is an international experience and as the first Finnish team, we went to find advice and experience around the globe. We contributed to the iGEM community by building a <a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation#Seekers">BioBrick Seeker</a> tool which makes it easy to find parts of your interest in the 2014 BioBrick distribution. This tool has been used by iGEM teams all over the world! We also did a lot of <a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation#Interteam">cooperation</a>, including filling out surveys and having skype conversations and live meetings with current and previous iGEM teams from France, Colombia, Switzerland, USA and the Netherlands. We even made a <a href="http://www.youtube.com/embed/icPgP3OOVOQ?rel=0">video</a> together with ETH Zürich team to Colombia team’s challenge.
 +
</p>
 +
<p>
 +
We wanted to <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach">raise awareness</a> about synthetic biology in Finland and also showcase researchers’ work to young people and undergraduate students. Therefore, we have been reaching out to general public, especially young people via <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach#SoMe">social media</a>, including <a href="http://www.facebook.com/AaltoHelsinki">Facebook</a>, <a href="http://twitter.com/AaltoHelsinki">Twitter</a>, <a href="http://www.youtube.com/channel/UCEZliqjLu86CRpQlk57FfSw">Youtube</a>, <a href="http://www.flickr.com/photos/aaltohelsinki/">Flickr</a> and our own blog. We’ve shared stories, pictures, videos and experiences to our followers. In addition, we have been featured in radio interviews and articles on magazines and webzines. We also made a silly game: <a href="https://2014.igem.org/Team:Aalto-Helsinki/Flappycoli">Flappy Coli</a>. We have a public team webpage, <a href="http://2014.aaltohelsinki.com/">aaltohelsinki.com</a>, where you can find the latest stories of Aalto Helsinki also in the future. We lifted some of the highlights of this project on a <a href="https://2014.igem.org/Team:Aalto-Helsinki/Journal#Timeline">timeline</a>: the story of Aalto-Helsinki Bioworks is there for you to see!
 +
</p>
 +
</div>
 +
<div class="col-md-6 img-100">
 +
<img src="https://static.igem.org/mediawiki/2014/a/a2/Aalto_Helsinki_Banner.png" class="img-responsive">
 +
</div>
 +
</div>
-
<h2>Project Description</h2>
 
-
<p>
 
-
We are the first-ever Finnish iGEM Team and one of the three teams in the new entrepreneurship track. Our aim is to build active synthetic biology community in Finland, to learn and share what biotech entrepreneurship takes
 
-
and to find an open way to build business on synthetic biology.
 
-
</p>
 
-
<p>
 
-
We are engineering bacteria whose gene expression can be controlled with blue light. In contrast to previous research, our remote controller is based on light intensity instead of different wavelengths. Thus, we have created a multi-channel switch that requires only one wavelength.
 
-
</p>
 
-
<p>
 
-
Our device could be used in bioreactors, making prototyping and multi-phase production chains faster, easier and more profitable. We’re making a proof-of-concept controller prototype for research teams, but it can be further scaled up for industrial purposes, such as medical and biofuel industry.
 
-
</p>
 
-
<p>
 
-
Follow our work on <a href="http://twitter.com/AaltoHelsinki">Twitter</a>, <a href="http://www.facebook.com/AaltoHelsinki">Facebook</a>, <a href="http://www.aaltohelsinki.com/blog/">blog</a> and <a href="http://aaltohelsinki.com/index_en.html">team web page</a>.
 
-
</p>
 
</article>
</article>
<article>
<article>
-
<h2>Qualifications</h2>
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<h2 class="kakspaddingbot">Accomplishments</h2>
-
<p>
+
<ul>
-
Coming soon(tm).
+
<li>Best undergraduate wiki 2014</li>
-
</p>
+
<li>Poster</li>
 +
<li>Presentation at the Giant Jamboree in Boston</li>
 +
<li>Background research, design and the creation of a <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research">Three-Channel Gene Switch</a></li>
 +
<li>Submission of <a href="https://2014.igem.org/Team:Aalto-Helsinki/Research#Parts">three new BioBricks and two new primers to the Registry</a></li>
 +
<li>Creation of <a href="https://2014.igem.org/Team:Aalto-Helsinki/Business#Plan">an Open Source business model and a business plan</a> for Synthetic Biology Entrepreneurship</li>
 +
<li>Logo and brand design</li>
 +
<li><a href="http://youtu.be/tlc7MPY9SE8">Pitch</a> to an audience of 1000+ people</li>
 +
<li>Participation in the <a href="https://2014.igem.org/Team:Aalto-Helsinki/Business#Sos">Summer of Startups</a>, a startup incubator program</li>
 +
<li>Connecting with <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach">investors and general public</a></li>
 +
<li>Received 45 000€ in <a href="https://2014.igem.org/Team:Aalto-Helsinki/Team#Sponsors">funding</a></li>
 +
<li>Drawing attention <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach#Media">in the media</a></li>
 +
<li>BioBrick and Team <a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation#Seekers">Seekers</a></li>
 +
<li>Collaboration with other <a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation#Interteam">iGEM teams</a></li>
 +
<li>Participation in the <a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation#Interlab">Measurement Interlab Study</a></li>
 +
</ul>
</article>
</article>
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<p>
<p>
-
Our wiki has seven different categories in addition to this main page. On each of them you can just scroll through the content until you're finished reading. Everything about a subject can be found on one page, additional material is provided when possible and pages that discuss similar stuff are linked to each other.
+
Our wiki has seven different categories in addition to this main page. On each of them you can just scroll through the content until you're finished reading. Everything about the subject can be found on one page, additional material is provided when possible and pages discussing similar topics are linked to each other. A submenu will also appear when you start scrolling through the other pages: see the top of the page as you advance in the wiki!
</p>
</p>
-
<p>
 
-
The seven categories are Team, Business, Research, Modeling, Cooperation, Outreach and Journal. If you find yourself lost (or simply don't know where to start looking for what you'd want to find), <a href="https://static.igem.org/mediawiki/2014/7/73/Aalto_Helsinki_Wiki_Sitemap.png">here's a sitemap to help with the task</a>!
 
-
</p>
 
-
 
<div class="row row-eq-height">
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<p class="feattext-p">
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We are a <strong>Team</strong> of nine Finnish students and we've gotten amazing support during this project.
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<big><big><big>Team</big></big></big><br>
 +
We are a team of nine Finnish students and we've gotten amazing support during this project.
</p>
</p>
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  <div class ="feattext-cell">
<p class="feattext-p">
<p class="feattext-p">
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We explored the possibilities of biotech and synthetic biology <strong>Business</strong>.
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<big><big><big>Business</big></big></big><br>
 +
We explored the possibilities of biotech and synthetic biology business.
</p>
</p>
</div>
</div>
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  <div class ="feattext-cell">
<p class="feattext-p">
<p class="feattext-p">
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We <strong>Researched</strong> and engineered a three-channel switch that can be controlled with the intensity of light.
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<big><big><big>Research</big></big></big><br>
 +
We engineered a three-channel switch that can be controlled with the intensity of blue light.
</p>
</p>
</div>
</div>
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<div class="row row-eq-height">
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<div class="col-md-6 bg feat feat-left bg-modeling">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Modeling">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Modeling">
<div class="feattext-container-tall">
<div class="feattext-container-tall">
  <div class ="feattext-cell">
  <div class ="feattext-cell">
<p class="feattext-p">
<p class="feattext-p">
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We studied and made mathematic <strong>Models</strong> of the interactions behind our gene switch.
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<big><big><big>Modeling</big></big></big><br>
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We made mathematic models of the interactions behind our gene switch.
</p>
</p>
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</a>
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<a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Cooperation">
<div class="feattext-container-tall">
<div class="feattext-container-tall">
  <div class ="feattext-cell">
  <div class ="feattext-cell">
<p class="feattext-p">
<p class="feattext-p">
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We created tools to make research easier, participated in the Interlab Study and <strong>Cooperated</strong> with other iGEM teams.
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<big><big><big>Cooperation</big></big></big><br>
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We got to know other iGEM teams and developed tools for everyone to use.
</p>
</p>
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<div class="row row-eq-height">
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<div class="col-md-6 bg feat feat-left bg-outreach">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach">
<div class="feattext-container-tall">
<div class="feattext-container-tall">
  <div class ="feattext-cell">
  <div class ="feattext-cell">
<p class="feattext-p">
<p class="feattext-p">
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We spread the word about iGEM and synthetic biology especially in Finland and conducted <strong>Outreach</strong> with the general public.
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<big><big><big>Outreach</big></big></big><br>
 +
We spread the word about iGEM and synthetic biology especially in Finland.
</p>
</p>
</div>
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</a>
</div>
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<div class="col-md-6 bg feat feat-right bg-journal">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Journal">
<a href="https://2014.igem.org/Team:Aalto-Helsinki/Journal">
<div class="feattext-container-tall">
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We documented the project well. You can find everything, and a bit more, from our <strong>Journal</strong>.
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<big><big><big>Journal</big></big></big><br>
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We documented the project well.
</p>
</p>
</div>
</div>
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</p>
</div>
</div>
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<p>
 +
If you find yourself lost (or simply don't know where to start looking for what you'd want to find), <a href="https://static.igem.org/mediawiki/2014/7/73/Aalto_Helsinki_Wiki_Sitemap.png" target="_blank">here's a sitemap to help with the task</a>!
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</p>
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</article></div>
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</article>
 +
</div>
</body>
</body>
<script>
<script>
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     Do not put href attribute, it will be replaced with a link to the image.
     Moreover, inner html of the anchor tag will be "figure x" where x is the
     Moreover, inner html of the anchor tag will be "figure x" where x is the
-
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     correct figure number. If span or anchor has "." as the inner html, the
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     */
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                     e2.innerHTML = "figure " + (index+1);
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    USAGE:
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    tag table with a tab that is connected to the table, such as:
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    <table ref="intensities" />
 +
    When you insert a reference in text, put a tag with a ref attribute:
 +
    <a tab="intensities"></a>
 +
    Do not put href attribute, it will be replaced with a link to the image.
 +
    Moreover, inner html of the anchor tag will be "table x" where x is the
 +
    correct table number. If span or anchor has "." as the inner html, the
 +
    caption will be capitalized.
 +
    */
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    var tables = $('table[tab], img[tab]');
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    $.each(tables, function(index, e){
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Latest revision as of 02:07, 9 June 2015

Aalto-Helsinki
Bioworks

Figuring out how to combine business with science.

Aalto-Helsinki Bioworks
A Synthetic Biology Startup with a Three-Channel Gene Switch

Project Description

We have engineered a three-channel switch that is controlled with the intensity of blue light. By utilizing the mechanisms of the lambda repressor, we are able to switch between the expressions of three different genes with a short delay. This kind of mechanism provides a nearly real-time control over genes, which could provide advantages in variety of industrial bioprocesses.

Inspired by the principles of open source software, we introduce an Open Source business model implemented around our switch. We want to encourage future companies to create Open Source based solutions and to empower customers to participate in the product development. Open Source model’s transparency as well as lower degree of protection raises trust and continuity and benefits larger community.

We want to share our experiences and insights in biotech entrepreneurship and address the difficulties that students and newcomers may face in the early stages of building a synthetic biology startup.

Our pitch video on August 6th, Summer of Startups Demo Day.

The Story of Aalto-Helsinki Bioworks

We are Aalto-Helsinki Bioworks, the first-ever Finnish iGEM Team and one of the four teams in the new Entrepreneurship Track. Our team consists of nine students from Aalto University and the University of Helsinki and we are combining our interdisciplinary forces to develop something new and fascinating. We want to put Finland on the map of synthetic biology and improve undergraduate research opportunities in our universities.

We have designed a three-channel gene switch that would make it possible to control three user-defined genes with blue light intensity. We are using YF1 as our light receptor protein and the signal is mediated to lambda repressor protein (CI) production via phosphorylation pathway. The concentration of the CI protein defines the gene that should be active at a time. The secret behind this function is our modified version of the lambda repressor mechanism.

We constructed a mathematical model that simulates the interactions of the molecules and function of the gene switch we designed. This revealed interesting phenomena in the dynamics of our system which helps us to better understand the capabilities and limitations of the gene switch. We also made an interactive simulation available to everybody to best illustrate how our idea actually works.

To control the amount of blue light on our cell cultures, we created the LED rig device. This enabled us to perform diverse experiments in order to characterize our light response element. These results show that our light response element is able to regulate the downstream gene expression precisely. In addition, the response time is in a ten-minute-scale, which enables constant and nearly real-time control over bacterial cultures. In addition, we hypothesize that this system is also applicable to bioreactors, which would enable higher level of control in industrial bioprocesses. However, our fully functional prototype is still under ongoing development.

We have developed an open approach to biotech business inspired by the business models for open source software. Following the Open Source philosophy, we will distribute open Aalto-Helsinki Bioworks technologies for free to everybody - as long as they share the improvements made on the technology. Therefore, we empower our users to participate in product development. Our business model is based on hardware and wide range of services offered to our customers.

During the summer we participated in Aalto Entrepreneurship Society’s Summer of Startups 2014 incubator programme to learn the essential entrepreneurial skills, such as pitching, investor relations, customer-driven development and explaining the science for general audience. See our Demo Day video for details.

iGEM is an international experience and as the first Finnish team, we went to find advice and experience around the globe. We contributed to the iGEM community by building a BioBrick Seeker tool which makes it easy to find parts of your interest in the 2014 BioBrick distribution. This tool has been used by iGEM teams all over the world! We also did a lot of cooperation, including filling out surveys and having skype conversations and live meetings with current and previous iGEM teams from France, Colombia, Switzerland, USA and the Netherlands. We even made a video together with ETH Zürich team to Colombia team’s challenge.

We wanted to raise awareness about synthetic biology in Finland and also showcase researchers’ work to young people and undergraduate students. Therefore, we have been reaching out to general public, especially young people via social media, including Facebook, Twitter, Youtube, Flickr and our own blog. We’ve shared stories, pictures, videos and experiences to our followers. In addition, we have been featured in radio interviews and articles on magazines and webzines. We also made a silly game: Flappy Coli. We have a public team webpage, aaltohelsinki.com, where you can find the latest stories of Aalto Helsinki also in the future. We lifted some of the highlights of this project on a timeline: the story of Aalto-Helsinki Bioworks is there for you to see!

Accomplishments

Categories

Our wiki has seven different categories in addition to this main page. On each of them you can just scroll through the content until you're finished reading. Everything about the subject can be found on one page, additional material is provided when possible and pages discussing similar topics are linked to each other. A submenu will also appear when you start scrolling through the other pages: see the top of the page as you advance in the wiki!

If you find yourself lost (or simply don't know where to start looking for what you'd want to find), here's a sitemap to help with the task!