iGEM Team Aachen BioBricks
RFC [25] Version of E0020
This part is a RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.
RFC [25] -compatible dark quencher based on K1319000 (E0030)
This part is a RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
Two mutations were introduced that eliminated fluorescence:
RFC [25] -compatible dark quencher based on K1319000 (E0030)
This part is a RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP).
Three mutations were introduced that eliminated fluorescence:
human galectin-3, codon optimized for E. coli
Galectin-3 is a 26 kDa protein that binds certain LPS patterns.
TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.
Translational unit of mRFP-galectin3-his
This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015).
IPTG inducible iLOV
This part can be used for IPTG-induced expression of K660004 (iLOV).
- Part Name
- Part type
- Creator
- Sequence
- Short Description (60 characters on what the DNA does)
- Long Description (Longer description of what the DNA does)
- Design considerations
<groupparts>iGEM14 Aachen</groupparts>
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