Team:Aachen/Parts

From 2014.igem.org

(Difference between revisions)
(→Constitutive expression of GFP-eYFP fusion protein)
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The coding sequence encodes EYFP (enhanced yellow fluorescent protein) which is derived from ''A. victoria'' GFP. The excitation is 512 nm and the emission is 534 nm.
The coding sequence encodes EYFP (enhanced yellow fluorescent protein) which is derived from ''A. victoria'' GFP. The excitation is 512 nm and the emission is 534 nm.
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==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==
==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==
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*L90I
*L90I
*Y145W
*Y145W
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319002 K1319002]==
==[http://parts.igem.org/Part:BBa_K1319002 K1319002]==
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*Y145W
*Y145W
*H148R
*H148R
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319003 K1319003]==
==[http://parts.igem.org/Part:BBa_K1319003 K1319003]==
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The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.
The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==
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The TEV protease is commonly used as a biochemical tool to cleave affinity tags from purified proteins like [http://parts.igem.org/Part:BBa_K1319007 His-Tags]. The high specifity makes the protease relatively non-toxic ''in vitro'' and ''in vivo''. The molecular weight of the TEV protease is 27 kDa.
The TEV protease is commonly used as a biochemical tool to cleave affinity tags from purified proteins like [http://parts.igem.org/Part:BBa_K1319007 His-Tags]. The high specifity makes the protease relatively non-toxic ''in vitro'' and ''in vivo''. The molecular weight of the TEV protease is 27 kDa.
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319008 K1319008]==
==[http://parts.igem.org/Part:BBa_K1319008 K1319008]==
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This protein generator produces [http://parts.igem.org/Part:BBa_K1319004 TEV protease] when induced with IPTG in a DE3 strain or if combined with a T7 RNA-Polymerase generator.
This protein generator produces [http://parts.igem.org/Part:BBa_K1319004 TEV protease] when induced with IPTG in a DE3 strain or if combined with a T7 RNA-Polymerase generator.
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319010 K1319010]==
==[http://parts.igem.org/Part:BBa_K1319010 K1319010]==
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This part expresses K1319000 behind a J23101 constitutive promotor.
This part expresses K1319000 behind a J23101 constitutive promotor.
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319011 K1319011]==
==[http://parts.igem.org/Part:BBa_K1319011 K1319011]==
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This part expresses K1319001 behind a J23101 constitutive promotor.
This part expresses K1319001 behind a J23101 constitutive promotor.
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{{Team:Aachen/BlockSeparator}}
== [http://parts.igem.org/Part:BBa_K1319012 K1319012] ==
== [http://parts.igem.org/Part:BBa_K1319012 K1319012] ==
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This part expresses K1319002 behind a J23101 constitutive promotor.
This part expresses K1319002 behind a J23101 constitutive promotor.
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{{Team:Aachen/BlockSeparator}}
== [http://parts.igem.org/Part:BBa_K1319013 K1319013] ==
== [http://parts.igem.org/Part:BBa_K1319013 K1319013] ==
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This part expresses a E0040.K1319001 fusion protein (GFP-REACh1) behind a J23101 promotor.
This part expresses a E0040.K1319001 fusion protein (GFP-REACh1) behind a J23101 promotor.
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{{Team:Aachen/BlockSeparator}}
== [http://parts.igem.org/Part:BBa_K1319014 K1319014] ==
== [http://parts.igem.org/Part:BBa_K1319014 K1319014] ==
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This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promotor.
This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promotor.
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{{Team:Aachen/BlockSeparator}}
== [http://parts.igem.org/Part:BBa_K1319015 K1319015] ==
== [http://parts.igem.org/Part:BBa_K1319015 K1319015] ==
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This part expresses a E0040.K1319000 fusion protein (GFP-EYFP) behind a J23101 promotor.
This part expresses a E0040.K1319000 fusion protein (GFP-EYFP) behind a J23101 promotor.
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{{Team:Aachen/BlockSeparator}}
== [http://parts.igem.org/Part:BBa_K1319016 K1319016] ==
== [http://parts.igem.org/Part:BBa_K1319016 K1319016] ==
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ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319017 K1319017]==
==[http://parts.igem.org/Part:BBa_K1319017 K1319017]==
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This device produces iLOV (K660004) in response to a quorum sensing input.
This device produces iLOV (K660004) in response to a quorum sensing input.
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==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==
==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==
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This part is a translational unit of a mRFP-galectin-3-His (B0032.E1010.K1319003.K1319016.B0015).
This part is a translational unit of a mRFP-galectin-3-His (B0032.E1010.K1319003.K1319016.B0015).
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{{Team:Aachen/BlockSeparator}}
==[http://parts.igem.org/Part:BBa_K1319042 K1319042]==
==[http://parts.igem.org/Part:BBa_K1319042 K1319042]==

Revision as of 10:46, 15 October 2014

iGEM Team Aachen BioBricks

K1319000

RFC [25] Version of E0020

This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.

The coding sequence encodes EYFP (enhanced yellow fluorescent protein) which is derived from A. victoria GFP. The excitation is 512 nm and the emission is 534 nm.


K1319001

RFC [25] - compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Two mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W

K1319002

RFC [25] - compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence:

  • L90I
  • Y145W
  • H148R

K1319003

human galectin-3, codon optimized for E. coli

The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.

K1319004

TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

This part is a TEV protease in RFC25 that was codon-optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.

The TEV Protease (also known as Tobaco Edge Virus nuclear inclusion a endopeptidase) is a highly sequence specific cysteine protease from the Tobacco Edge Virus (TEV). The protease is highly sequence specific. The consensus sequence for the cut is ENLYFQ\S with \ denoting the cleaved peptide bond. This sequence can be found in the part K1319016.

ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.

The TEV protease is commonly used as a biochemical tool to cleave affinity tags from purified proteins like His-Tags. The high specifity makes the protease relatively non-toxic in vitro and in vivo. The molecular weight of the TEV protease is 27 kDa.

K1319008

IPTG-induced and T7-driven expression of TEV protease

This protein generator produces TEV protease when induced with IPTG in a DE3 strain or if combined with a T7 RNA-Polymerase generator.

K1319010

Constitutive expression of K1319000

This part expresses K1319000 behind a J23101 constitutive promotor.

K1319011

Constitutive expression of K1319001

This part expresses K1319001 behind a J23101 constitutive promotor.

K1319012

Constitutive expression of K1319002

This part expresses K1319002 behind a J23101 constitutive promotor.

K1319013

Constitutive expression of GFP-REACh1 fusion protein

This part expresses a E0040.K1319001 fusion protein (GFP-REACh1) behind a J23101 promotor.

K1319014

Constitutive expression of GFP-REACh2 fusion protein

This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promotor.

K1319015

Constitutive expression of GFP-EYFP fusion protein

This part expresses a E0040.K1319000 fusion protein (GFP-EYFP) behind a J23101 promotor.

K1319016

TEV protease cleavage site

This sequence codes for a TEV protease cleavage site.

ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.

K1319017

LasI induced iLOV

This device produces iLOV (K660004) in response to a quorum sensing input.

K1319020

Translational unit of mRFP-galectin3-His

This part is a translational unit of a mRFP-galectin-3-His (B0032.E1010.K1319003.K1319016.B0015).

K1319042

IPTG inducible iLOV

This part can be used for IPTG-induced expression of K660004 (iLOV).



  1. Part Name
  2. Part type
  3. Creator
  4. Sequence
  5. Short Description (60 characters on what the DNA does)
  6. Long Description (Longer description of what the DNA does)
  7. Design considerations


<groupparts>iGEM14 Aachen</groupparts>

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