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- | __NOTOC__
| + | #REDIRECT [[Team:Aachen/Notebook/Wetlab]] |
- | {{Team:Aachen/Header}}
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- | | + | |
- | = April =
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- | == 1st ==
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- | '''Organization'''
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- | where to get:
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- | * key cards and keys for the lab
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- | * cooled centifuge
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- | * space in -80 °C
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- | * bottles for 70 % ethanol
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- | * ... (problems of a first year team ^^)
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- | [https://2014.igem.org/Team:Aachen/Wetlab/April Read more about April here...] | + | |
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- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = May =
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- | == 1st ==
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- | * gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
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- | *: → 120 V, 30 min
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- | *: → cut out the bands
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- | [https://2014.igem.org/Team:Aachen/Wetlab/May Read more about May here...] | + | |
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- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = June =
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- | == 3rd ==
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- | * transformation of 34 BioBricks
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- | == 4th ==
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- | * preperation of consumables:
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- | ** fresh 50 % glycerol
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- | ** new LB plates with cam and with kanamycin (kan)
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- | ** 60 glas tubes
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- | ** 2 L LB<sup>-</sup>
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- | ** 100 mL steril glas beads for plating
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- | * master plates (6 clones per BioBrick) were made
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- | [https://2014.igem.org/Team:Aachen/Wetlab/June Read more about June here...]
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- | {{Team:Aachen/BlockSeparator}}
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- | | + | |
- | = July =
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- | == 1st ==
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- | * transformation efficiency kit is broken
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- | → run an agarose gel with the kit plasmids to check for nuclease activity/ bands
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- | * inventory of the -80°C freezer was done
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- | == 2nd ==
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- | * overnight cultures (LB) of K731520 and K1319042 for chips were inoculated
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- | [https://2014.igem.org/Team:Aachen/Wetlab/July Read more about July here...]
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- | {{Team:Aachen/BlockSeparator}}
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- | {{Team:Aachen/Footer}}
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