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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = April =
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| - | == 1st ==
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| - | '''Organization'''
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| - |
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| - | where to get:
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| - | * key cards and keys for the lab
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| - | * cooled centifuge
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| - | * space in -80 °C
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| - | * bottles for 70 % ethanol
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| - | * ... (problems of a first year team ^^)
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April Read more about April here...]
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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = May =
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| - | == 1st ==
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| - | * gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
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| - | *: → 120 V, 30 min
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| - | *: → cut out the bands
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May Read more about May here...]
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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = June =
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| - | == 3rd ==
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| - | * transformation of 34 BioBricks
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| - |
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| - | == 4th ==
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| - |
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| - | * preparation of consumables:
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| - | ** fresh 50 % glycerol
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| - | ** new LB plates with cam and with kanamycin (kan)
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| - | ** 60 glas tubes
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| - | ** 2 L LB<sup>-</sup>
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| - | ** 100 mL steril glas beads for plating
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| - |
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| - | * master plates (6 clones per BioBrick) were made
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June Read more about June here...]
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| - |
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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = July =
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| - | == 1st ==
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| - | * transformation efficiency kit is broken
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| - | → run an agarose gel with the kit plasmids to check for nuclease activity/ bands
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| - | * inventory of the -80°C freezer was done
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| - |
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| - | == 2nd ==
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| - | * overnight cultures (LB) of K731520 and K1319042 for chips were inoculated
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July Read more about July here...]
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| - |
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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = August =
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| - | == 1st ==
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| - | * made electrocompetent ''E.coli'' rosetta cells.
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| - | * prepared cultures of K1319042 and K131026
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| - |
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| - | == 2nd ==
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| - | * tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
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| - | * tested K131026 and K1319042 for fluorescence in the plate reader
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| - | * did a heat shock transformation of I746909 into NEB TOP 10 cells
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| - | * did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August Read more about August here...]
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| - |
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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = September =
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| - | == 1st ==
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| - | * 5 ml cultures of K1319003 and K1319004
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| - | * plasmid prep
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| - | {| class="wikitable"
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| - | |-
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| - | ! Plasmid !! DNA [ng/µl]
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| - | |-
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| - | | J23101.K516032 pSB1K3|| 23.5
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| - | |-
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| - | | J23115.K516032 pSB1K3|| 20.5
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| - | |-
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| - | | J04450 pSB1A3|| 57.5
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| - | |-
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| - | | J04450 pSB1K1|| 63.5
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| - | |}
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| - | * over night cultures of K131026 in DH5α and NEB
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September Read more about September here...]
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| - |
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| - | {{Team:Aachen/BlockSeparator}}
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| - |
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| - | = October =
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| - | == 1st ==
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| - | * Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the [https://2014.igem.org/Team:Aachen/Notebook/Protocols#Agar_Chips| sensor chip manufacturing] protocol:
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| - | ** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.
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| - |
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| - | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October Read more about October here...]
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| | {{Team:Aachen/Footer}} | | {{Team:Aachen/Footer}} |
"
