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Team:Aachen/Notebook/Wetlab2
From 2014.igem.org
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* ... (problems of a first year team ^^) | * ... (problems of a first year team ^^) | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/April Read more about April here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April Read more about April here...] |
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*: → cut out the bands | *: → cut out the bands | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/May Read more about May here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/May Read more about May here...] |
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* master plates (6 clones per BioBrick) were made | * master plates (6 clones per BioBrick) were made | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/June Read more about June here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/June Read more about June here...] |
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* overnight cultures (LB) of K731520 and K1319042 for chips were inoculated | * overnight cultures (LB) of K731520 and K1319042 for chips were inoculated | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/July Read more about July here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/July Read more about July here...] |
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* did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta | * did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/August Read more about August here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/August Read more about August here...] |
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* over night cultures of K131026 in DH5α and NEB | * over night cultures of K131026 in DH5α and NEB | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/September Read more about September here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/September Read more about September here...] |
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** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker. | ** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker. | ||
| - | [https://2014.igem.org/Team:Aachen/Wetlab/October Read more about October here...] | + | [https://2014.igem.org/Team:Aachen/Notebook/Wetlab/October Read more about October here...] |
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Revision as of 09:04, 8 October 2014
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