Team:Aachen/Notebook/Protocols

From 2014.igem.org

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{{Team:Aachen/Header}}
{{Team:Aachen/Header}}
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__TOC__
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= Media =
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== LB medium ==
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== Hartmans minimal medium (HM) ==
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= Agar Chips =
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= Transformation =
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== Heat Shock ==
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# thaw cells on ice
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# add 1 µl of plasmid DNA
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# incubate on ice for 30'
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# heat shock at 42 °C for 60''
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# incubate on ice for 5'
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# add 200 µl of SOC media
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# incubate at 37 °C for 2 h
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# plate 20  and 200 µl on plates with the appropiate antibiotic
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== Electroporation ==
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= PCR =
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== Colony PCR ==
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== QuikChange ==
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= Clonings =
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== Restriction Digest ==
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== Ligation ==
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== Gibson Assembly ==
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{{Team:Aachen/Footer}}
{{Team:Aachen/Footer}}

Revision as of 09:03, 12 August 2014

Contents


Media

LB medium

Hartmans minimal medium (HM)

Agar Chips

Transformation

Heat Shock

  1. thaw cells on ice
  2. add 1 µl of plasmid DNA
  3. incubate on ice for 30'
  4. heat shock at 42 °C for 60
  5. incubate on ice for 5'
  6. add 200 µl of SOC media
  7. incubate at 37 °C for 2 h
  8. plate 20  and 200 µl on plates with the appropiate antibiotic

Electroporation

PCR

Colony PCR

QuikChange

Clonings

Restriction Digest

Ligation

Gibson Assembly

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