Team:Aachen/Notebook/Protocols
From 2014.igem.org
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= Media = | = Media = | ||
== LB medium == | == LB medium == | ||
+ | # weight components | ||
+ | ** '''5 g/L NaCl''' | ||
+ | ** '''10 g/L tryptone''' | ||
+ | ** '''5 g/L yeast extract''' | ||
+ | ** (15 g/L Agar for plates) | ||
+ | # fill up to 1 L with deionized water | ||
+ | # '''mix well''' by shaking | ||
+ | # autoclave | ||
+ | ## autoclaving tape, caps slightly unscrewed | ||
+ | ## base of the pot has to be covered with deionized water | ||
+ | ## close lid | ||
+ | ## heat '''level 3 until the pressure valve opens''' | ||
+ | ## reduce '''heat level to 1.5''' | ||
+ | ## set timer to '''20 minutes''' | ||
+ | ## turn heater off | ||
+ | ## '''wait until the pressure valve retracts''' (30-45 minutes) | ||
+ | ## open, close caps & shake | ||
+ | # for plates, wait until you can touch the bottle ('''<60 °C''', clean bench!) | ||
+ | # add antibiotics and '''shake''' (gloves!) | ||
== Hartmans minimal medium (HM) == | == Hartmans minimal medium (HM) == |
Revision as of 09:15, 12 August 2014
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