Team:Aachen/Notebook/Protocols
From 2014.igem.org
(Difference between revisions)
(→M9 minimal medium (M9)) |
(→M9 minimal medium (M9)) |
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| 10x Salt solution || style="text-align:right"| 100 mL | | 10x Salt solution || style="text-align:right"| 100 mL | ||
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- | | Magnesiumsulfatehaptahydrate (10 mM) || 100 mL | + | | Magnesiumsulfatehaptahydrate (10 mM) || style="text-align:right"| 100 mL |
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- | | Glucose 20% (w/v) || 20 mL | + | | Glucose 20% (w/v) || style="text-align:right"| 20 mL |
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- | | 1000x Trace elements || 1 mL | + | | 1000x Trace elements || style="text-align:right"| 1 mL |
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- | | Thiamin (1 mM) || 0.333 mL | + | | Thiamin (1 mM) || style="text-align:right"| 0.333 mL |
|} | |} | ||
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{| class="wikitable" | {| class="wikitable" | ||
- | ! <center>'''Component'''</center> !! <center>'''Final | + | ! <center>'''Component'''</center> !! <center>'''Final concentration'''</center> !! <center>'''Concentration in stock solution'''</center> |
|- | |- | ||
- | | BisTris || 95mM || 200,000mg/L | + | | BisTris || style="text-align:right"| 95mM || style="text-align:right"| 200,000mg/L |
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- | | Ammmonium chloride || 60 mM || 32,100 mg/L | + | | Ammmonium chloride || style="text-align:right"| 60 mM || style="text-align:right"| 32,100 mg/L |
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- | | Sodium citrate || 12.5 mM || 27,000 mg/L | + | | Sodium citrate || style="text-align:right"| 12.5 mM || style="text-align:right"| 27,000 mg/L |
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- | | Monopotassium phosphate || 3 mM || 4,170 mg/L | + | | Monopotassium phosphate || style="text-align:right"| 3 mM || style="text-align:right"| 4,170 mg/L |
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- | | Dipotassium phosphate || 0.7 mM || 1,590 mg/L | + | | Dipotassium phosphate || style="text-align:right"| 0.7 mM || style="text-align:right"| 1,590 mg/L |
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|} | |} | ||
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{| class="wikitable" | {| class="wikitable" | ||
- | ! <center>'''Component'''</center> !! <center>'''Final | + | ! <center>'''Component'''</center> !! <center>'''Final concentration'''</center> !! <center>'''Concentration in stock solution'''</center> |
|- | |- | ||
- | | Iron(III) chloride || 50 mM || 13,515 mg/L | + | | Iron(III) chloride || style="text-align:right"| 50 mM || style="text-align:right"| 13,515 mg/L |
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- | | Calcium chloride || 20 mM || 2,220 mg/L | + | | Calcium chloride || style="text-align:right"| 20 mM || style="text-align:right"| 2,220 mg/L |
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- | | Manganese(II) chloride || 10 mM || 1,258 mg/L | + | | Manganese(II) chloride || style="text-align:right"| 10 mM || style="text-align:right"| 1,258 mg/L |
|- | |- | ||
- | | Zinc sulfate || 10 mM || 1,615 mg/L | + | | Zinc sulfate || style="text-align:right"| 10 mM || style="text-align:right"| 1,615 mg/L |
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- | | Cobalt(II) chloride || 2 mM || 260 mg/L | + | | Cobalt(II) chloride || style="text-align:right"| 2 mM || style="text-align:right"| 260 mg/L |
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- | | Copper(II) chloride || 2 mM || 269 mg/L | + | | Copper(II) chloride || style="text-align:right"| 2 mM || style="text-align:right"| 269 mg/L |
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- | | Nickel(II) chloride || 2 mM || 259 mg/L | + | | Nickel(II) chloride || style="text-align:right"| 2 mM || style="text-align:right"| 259 mg/L |
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- | | Sodium molybdate || 2 mM || 412 mg/L | + | | Sodium molybdate || style="text-align:right"| 2 mM || style="text-align:right"| 412 mg/L |
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- | | Sodium selenite || 2 mM || 346 mg/L | + | | Sodium selenite || style="text-align:right"| 2 mM || style="text-align:right"| 346 mg/L |
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- | | Boric acid || 2 mM || 124 mg/L | + | | Boric acid || style="text-align:right"| 2 mM || style="text-align:right"| 124 mg/L |
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- | | Hydrochloric acid || 1 mM || 20 mL | + | | Hydrochloric acid || style="text-align:right"| 1 mM || style="text-align:right"| 20 mL |
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|} | |} |
Revision as of 13:49, 8 October 2014
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