Team:Aachen/Notebook/Protocols
From 2014.igem.org
(Difference between revisions)
(→M9 minimal medium (M9)) |
(→M9 minimal medium (M9)) |
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Line 44: | Line 44: | ||
{| class="wikitable" | {| class="wikitable" | ||
- | ! <center>'''Components for 1 | + | ! <center>'''Components for 1 L'''</center> !! <center>'''Volume'''</center> |
|- | |- | ||
| bidest. water || 778.667 mL | | bidest. water || 778.667 mL | ||
Line 56: | Line 56: | ||
| 1000x Trace elements || 1 mL | | 1000x Trace elements || 1 mL | ||
|- | |- | ||
- | | Thiamin (1 mM) | + | | Thiamin (1 mM) || 0.333 mL |
|} | |} | ||
+ | '''10x Salt solution''' | ||
+ | {| class="wikitable" | ||
+ | ! <center>'''Component'''</center> !! <center>'''Final conc.'''</center> !! <center>'''Conc. in stock solution'''</center> | ||
+ | |- | ||
+ | | BisTris || 95mM || 200,000mg/L | ||
+ | |- | ||
+ | | \ce{NH4Cl} || 60 mM || 32,100 mg/L | ||
+ | |- | ||
+ | | \ce{Na}-Citrate || 12.5 mM || 27,000 mg/L | ||
+ | |- | ||
+ | | \ce{KH2PO4} || 3 mM || 4,170 mg/L | ||
+ | |- | ||
+ | | \ce{K2HPO4} || 0.7 mM || 1,590 mg/L | ||
+ | |- | ||
+ | |} | ||
+ | '''1000x Trace elements''' | ||
- | + | {| class="wikitable" | |
- | + | ! <center>'''Component'''</center> !! <center>'''Final conc.'''</center> !! <center>'''Conc. in stock solution'''</center> | |
- | + | |- | |
- | + | | \ce{FeCl3} || 50 mM || 13,515 mg/L | |
- | + | |- | |
- | + | | \ce{CaCl2} || 20 mM || 2,220 mg/L | |
- | + | |- | |
- | + | | \ce{MnCl2} || 10 mM || 1,258 mg/L | |
- | + | |- | |
- | + | | \ce{ZnSO4} || 10 mM || 1,615 mg/L | |
- | + | |- | |
- | + | | \ce{CoCl2} || 2 mM || 260 mg/L | |
- | + | |- | |
- | + | | \ce{CuCl2} || 2 mM || 269 mg/L | |
- | + | |- | |
- | + | | \ce{NiCl2} || 2 mM || 259 mg/L | |
- | + | |- | |
- | + | | \ce{Na2MoO4} || 2 mM || 412 mg/L | |
- | + | |- | |
- | + | | \ce{Na2SeO3} || 2 mM || 346 mg/L | |
- | + | |- | |
- | + | | \ce{H3BO3} || 2 mM || 124 mg/L | |
- | + | |- | |
- | + | | \ce{HCl} || 1 mM || 20 mL | |
- | + | |- | |
- | + | |} | |
- | + | ||
- | + | ||
== SOC == | == SOC == |
Revision as of 13:34, 8 October 2014
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