Team:Aachen/Notebook/Protocols
From 2014.igem.org
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{| class="wikitable" style="text-align: right;" | {| class="wikitable" style="text-align: right;" | ||
! | ! | ||
- | !! style="border-left: 2px solid #404040;" colspan="3"| | + | !! style="border-left: 2px solid #404040;" colspan="3"|0.75 mm 12 % RUNNING Gel |
- | + | ||
!! style="border-left: 2px solid #404040; background-color:#8ebae5;" colspan="3"|1 mm 4 % STACKING Gel | !! style="border-left: 2px solid #404040; background-color:#8ebae5;" colspan="3"|1 mm 4 % STACKING Gel | ||
|- | |- | ||
| | | | ||
- | |||
| style="border-left: 2px solid #404040;"| '''1x''' || '''2x''' || '''4x''' | | style="border-left: 2px solid #404040;"| '''1x''' || '''2x''' || '''4x''' | ||
| style="border-left: 2px solid #404040;"| '''1x''' || '''2x''' || '''4x''' | | style="border-left: 2px solid #404040;"| '''1x''' || '''2x''' || '''4x''' | ||
|- | |- | ||
| '''H{{sub|2}}O''' | | '''H{{sub|2}}O''' | ||
- | | style="border-left: 2px solid #404040;"| 1. | + | | style="border-left: 2px solid #404040;"| 1.65 mL || 3.3 mL || 6.6 mL |
- | + | | style="border-left: 2px solid #404040;"| 1.5 mL || 3 mL || 6 mL | |
- | | style="border-left: 2px solid #404040;"| 1. | + | |
|- | |- | ||
- | | ''' | + | | '''1.5x Gel Buffer''' |
- | | style="border-left: 2px solid #404040;"| 1. | + | | style="border-left: 2px solid #404040;"| 1.3 mL || 2.6 mL || 5.2 mL |
- | | | + | | style="border-left: 2px solid #404040;"| 0.65 mL || 1.3 mL || 2.6 mL |
- | | style="border-left: 2px solid #404040;"| 0. | + | |
|- | |- | ||
| '''30 % Acrylamide (37.5:1)''' | | '''30 % Acrylamide (37.5:1)''' | ||
- | | style="border-left: 2px solid #404040;"| 2 | + | | style="border-left: 2px solid #404040;"| 2 mL || 4 mL || 8 mL |
- | + | | style="border-left: 2px solid #404040;"| 0.325 mL || 0.65 mL || 1.3 mL | |
- | | style="border-left: 2px solid #404040;"| 0. | + | |
|- | |- | ||
| '''10 % APS''' | | '''10 % APS''' | ||
- | | style="border-left: 2px solid #404040;"| | + | | style="border-left: 2px solid #404040;"| 50 µL || 100 µL || 200 µL |
- | | style="border-left: 2px solid #404040;"| | + | | style="border-left: 2px solid #404040;"| 25 µL || 50 µL || 100 µL |
- | + | ||
|- | |- | ||
| '''TEMED''' | | '''TEMED''' | ||
- | | style="border-left: 2px solid #404040;"| | + | | style="border-left: 2px solid #404040;"| 10 µL || 20 µL || 40 µL |
- | | style="border-left: 2px solid #404040;"| | + | | style="border-left: 2px solid #404040;"| 5 µL || 10 µL || 20 µL |
- | + | ||
|- | |- | ||
|} | |} |
Revision as of 16:41, 7 October 2014
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