Team:Aachen/Notebook/Protocols
From 2014.igem.org
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== Electroporation == | == Electroporation == | ||
- | # add 1 | + | # add 1 μL plasmid to electrocompetent cells |
# put DNA/ cell suspension in electroporation cuvette | # put DNA/ cell suspension in electroporation cuvette | ||
# wipe dry the electroporator | # wipe dry the electroporator | ||
- | # use small plastic pipette to place the cells | + | # use a small plastic pipette to place the cells |
- | # | + | # pulse: 2.5 kV, 200-400 Ω, 25 μF (for ''E.coli'') |
- | # immediatly add 1 | + | # immediatly add 1 mL LB and incubate for 2 h at 37 °C |
- | # plate 50 | + | # plate 50 μL on selective medium plate |
- | # centrifuge the rest (3000 | + | # centrifuge the rest (3000 g, 20 min), discard supernatant, re-suspend the pellet in 50 μL LB and plate it on selective medium plate |
= PCR = | = PCR = |
Revision as of 07:05, 3 October 2014
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