Team:Aachen/Notebook/Protocols
From 2014.igem.org
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'''Cell preparation''' | '''Cell preparation''' | ||
- | # over night culture of sensor cells (50 | + | # over night culture of sensor cells (50 mL in a 250 mL flask with) max. 16 h |
- | # centrifuge all 50 | + | # centrifuge all 50 mL by 3000 g for 10 min. |
# discard the supernatant | # discard the supernatant | ||
- | # | + | # re-suspend the pellet in 1 mL medium at RT |
'''Agar preparation''' | '''Agar preparation''' | ||
- | # autoclave 50 | + | # autoclave 50 mL medium with 1.5 % agarose |
- | # cool it down to 45 °C in a water bath | + | # cool it down to 45 °C in a water bath |
'''Chip production''' | '''Chip production''' | ||
- | # mix the cooled | + | # mix the cooled medium with the cells by inverting gently |
- | # pour it in the chip form | + | # pour it in the chip form, avoiding bubble formation (!) |
- | # wait for ca 20 min | + | # wait for ca 20 min until the agar has solidified |
# cut out the chips with a scalpel | # cut out the chips with a scalpel | ||
# put ever two chips into a labeled petri dish | # put ever two chips into a labeled petri dish | ||
- | # incubate for | + | # incubate for 1 h at 37 °C |
= Transformation = | = Transformation = |
Revision as of 06:58, 3 October 2014
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