Team:Aachen/Notebook/Protocols
From 2014.igem.org
(Difference between revisions)
(→LB medium) |
(→TB medium) |
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== TB medium == | == TB medium == | ||
# components 1: | # components 1: | ||
- | : | + | #: '''4 mL/L glycerol''' |
- | : | + | #: '''12 g/L tryptone''' |
- | : | + | #: '''24 g/L yeast extract''' |
# fill up to 900 mL with deionized water | # fill up to 900 mL with deionized water | ||
# '''mix well''' by shaking | # '''mix well''' by shaking | ||
# autoclave | # autoclave | ||
# components 2: | # components 2: | ||
- | : | + | #: '''0.17 M KH<sub>2</sub>PO<sub>4</sub>''' |
- | : | + | #: '''0.72 M K<sub>2</sub>HPO<sub>4</sub>''' |
# dissolve in 100 mL deionized water and sterilize it by passing it through a filter | # dissolve in 100 mL deionized water and sterilize it by passing it through a filter | ||
# after autoclaving and cooling down, add sterile phosphate solutions | # after autoclaving and cooling down, add sterile phosphate solutions |
Revision as of 06:53, 3 October 2014
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