Team:Aachen/Notebook/Engineering/ODF
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=== Light filters === | === Light filters === | ||
- | Once the cuvette holder was finished, '''finding good filters was a tough challenge'''. A main goal throughout our project has been to choose easily available parts which are also inexpensive. Thus choosing Schott glasses as filters unfortunately could not be considered. Instead, filters used for illumination of theaters seemed to be ideal solution. | + | Once the cuvette holder was finished, '''finding good filters was a tough challenge'''. A main goal throughout our project has been to choose easily available parts which are also inexpensive. Thus choosing Schott glasses as filters unfortunately could not be considered. Instead, filters used for illumination of theaters seemed to be an ideal solution. |
Especially for the fluorescence measurements of GFP finding the right filter has been a big problem. [http://parts.igem.org/Part:BBa_E0040 GFPmut3b] has a peak excitation at 501 nm and a peak emission at 511 nm - too close together for our low-cost filters to block the excitation light but transmit the emitted light. Thus, we chose to excite at around 485 nm reduce false positive results below 500 nm. However, no adequate filter for these settings could be found. | Especially for the fluorescence measurements of GFP finding the right filter has been a big problem. [http://parts.igem.org/Part:BBa_E0040 GFPmut3b] has a peak excitation at 501 nm and a peak emission at 511 nm - too close together for our low-cost filters to block the excitation light but transmit the emitted light. Thus, we chose to excite at around 485 nm reduce false positive results below 500 nm. However, no adequate filter for these settings could be found. | ||
- | Eventually, using the dark greenish Twickenham Green filter only little amounts of light shorter than 500 nm | + | Eventually, using the dark greenish [filters.com/lighting/colour-details.html#736 Twickenham Green] filter only little amounts of light shorter than 500 nm gets through, reducing any bias from excitation illumination significantly. Unfortunately, the transmission rate of this filter is quite bad, 20 % only, for the target emission wavelength of 511 nm. |
- | For the OD measurement, too, we had similar problems. | + | For the OD measurement, too, we had similar problems. Indeed, due to our goal of inexpensive parts, we only filter light below 600 nm. Further filters would lower the base transmittance and result in a loss of resolution which is not tolerable. |
+ | Finally the red filter [http://leefilters.com/lighting/colour-details.html#019 Fire] permits over 70 r; of the light to the sensor and is thus suited for our purposes. | ||
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Revision as of 21:48, 13 October 2014
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