Team:Aachen/Labbook
From 2014.igem.org
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== 19th to 25th == | == 19th to 25th == | ||
* we transformed some BioBricks and did a colony PCR | * we transformed some BioBricks and did a colony PCR | ||
- | [[ | + | [[Image:Aachen_14-05-23_COLONY-PCR.jpg|thumb|200px|colony PCR picture]] |
== 26th to 31st == | == 26th to 31st == |
Revision as of 08:47, 16 July 2014
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More information will be available soon!
May
1st to 11th
Thu, 8th
- SOE-PCR step 2
12th to 18th
- ...
19th to 25th
- we transformed some BioBricks and did a colony PCR
26th to 31st
- ...
June
25th
- preculture for competent NEB10β cells
- submit samples for sequencing
- make masterplates of transformed BioBricks
- centrifuge and freeze overnight expression culture of J23101.E0240 and K516132
26th
- made competent NEB10β cells - several things went wrong.. we'll see (remember: pre-cool centrifuge, always check if it's indeed spinning, frequently check OD of the culture)
- colony PCR on the transformed clones looked awful. There were too many cells in the 10 µl reaction volume. Some seals weren't fully closed. Basically only primers and smear except for the positive control which contained a plasmid template instead of cells
- 2x 500 ml of LB and three sterile flasks have been made
July
16th
- plasmid preps
- transformation of different reporter strains
- ...
sample table:
BioBrick | Colonies after Transformation | Colony PCR result |
---|---|---|
E0240 | 5 | positive |
E0040 | 6 | negative |
August
...
September
...
October
...
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