Team:Aachen/Collaborations/Neanderlab
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Overall, this event was '''a great success'''! We good really good positive feedback from both students and instructors, and the project even made it into a local newspaper. ''Rheinische Post'' reported about the collaboration between our iGEM team and the NEAnderLab in the article [http://www.genios.de/presse-archiv/artikel/RP/20140924/schueler-forschen-mit-studenten-im-/20140924015746000040077524.html "Schüler forschen mit Studenten im Neanderlab"] ("High school students do research with university students in the Neanderlab") on September 24th, 2014. | Overall, this event was '''a great success'''! We good really good positive feedback from both students and instructors, and the project even made it into a local newspaper. ''Rheinische Post'' reported about the collaboration between our iGEM team and the NEAnderLab in the article [http://www.genios.de/presse-archiv/artikel/RP/20140924/schueler-forschen-mit-studenten-im-/20140924015746000040077524.html "Schüler forschen mit Studenten im Neanderlab"] ("High school students do research with university students in the Neanderlab") on September 24th, 2014. | ||
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+ | ===Summary of the Day=== | ||
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+ | The students carried out multiple '''experiments in context of our iGEM project'''. During the day, the students collected samples from an ''E. coli'' TB culture every 30 minutes to observe the exponential growth phase. The optical density was '''measured with our own DIY photometer'''. Overall, the students did a good job of treating their bacterial culture well, as shown in the graph below. | ||
+ | <center> | ||
+ | {{Team:Aachen/Figure|Aachen_14-09-24_Neanderlab_curves.png|align=center|title=Growth profiles of ''E. coli''|subtitle=Optical density of the cultures measured by the students|width=700px}} | ||
+ | </center> | ||
+ | In between the growth curve measurements, we '''introduced the iGEM competition and synthetic biology'''. Of course, our team members also explained the bacterial growth properties and how we can observe the propagation of cells using the photometer. After the sudden fire alarm and evacuation of the building, the rest of the day dealt with different types of '''"glowing" in nature''', including bioluminescence, fluorescence and phosphorescence. We started by explaining the mechanism behind bioluminescence in the context of quorum sensing of ''Vibrio fischeri'' in squid. As another example of glowing organisms we chose ''Pseudomonas fluorescens''. Students plated ''P. fluorescens'' onto Pseudomonas-F agar plates containing different concentrations of iron. These type of bacteria produce fluorescing siderophores when iron concentrations are low. After an incubation period of 1 to 2 days, the students should be able to see nice results in form of fluorescing and non-fluorescing bacterial colonies on their plates. | ||
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+ | The students also carried out some experiments from the [http://www.zdi-neanderlab.de/index.php?article_id=26/ NEAnderLab's workshops], such as ''Cold Light of the Deep Sea'', an experiment on chemiluminscence of luminol catalyzed by a copper wire. Playing around with glowing liquids of different colours in the dark was also a lot of fun! | ||
At the end of the day, we also answered the students' questions about synthetic biology and the Biology/Biotechnology university programs. As a souvenir, each student got to keep a phosphorescing stone. | At the end of the day, we also answered the students' questions about synthetic biology and the Biology/Biotechnology university programs. As a souvenir, each student got to keep a phosphorescing stone. |
Revision as of 20:50, 17 October 2014
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