Team:Aachen/Achievements
From 2014.igem.org
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- | We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] of reading out biosensors on a two dimensional level. | + | We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] of reading out biosensors on a two dimensional level. For this the sensor cells are immobilized in an agar chip. It was experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated] that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn'']. |
- | + | In order to improve the response of our sensor cells to induction we designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system] using a TEV protease to activate a reporter protein by cleaving off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by [https://2014.igem.org/Team:Aachen/Project/Model computational modeling] as well as in our [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements experiments]. All parts needed to build this kind of reporter system have been submitted to the parts registry. | |
{{Team:Aachen/BlockSeparator}} | {{Team:Aachen/BlockSeparator}} |
Revision as of 21:39, 17 October 2014
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