Team:AMU-Poznan/Project
From 2014.igem.org
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<div align="left">Bronze. The following 4 goals must be achieved:</br> | <div align="left">Bronze. The following 4 goals must be achieved:</br> | ||
<ul> | <ul> | ||
- | <li><b>Register the team, have a great summer, and have fun attending the Jamboree in Boston.</br>< | + | <li><b>Register the team, have a great summer, and have fun attending the Jamboree in Boston.</br></li> |
- | <li>Create and share a description of the team's project via the iGEM wiki.</br></b>< | + | <li>Create and share a description of the team's project via the iGEM wiki.</br></b></li> |
- | <li>Present a Poster and Talk at the Regional Jamboree and World Championship Jamboree.<b> We will on Jamboree :)</br>< | + | <li>Present a Poster and Talk at the Regional Jamboree and World Championship Jamboree.<b> We will on Jamboree :)</br></li> |
- | <li>Develop and make available, via The iGEM Software Registry, an open source software that supports Synthetic Biology based on Standard Parts.< | + | <li>Develop and make available, via The iGEM Software Registry, an open source software that supports Synthetic Biology based on Standard Parts.</li></b></br></br> |
</ul> | </ul> | ||
Silver: In addition to the Bronze Medal requirements, the following 4 goals must be achieved:</br> | Silver: In addition to the Bronze Medal requirements, the following 4 goals must be achieved:</br> |
Revision as of 17:00, 15 October 2014
sh-miR Designer v2.0Sh-miR designer is a project we started during iGEM 2013. This year we would like to continue and expand functionality of the software. sh-miR designer v1.0 (link) is aimed to create sh-miR molecules based on siRNAs provided by the user. In sh-miR designer v2.0 only the mRNA number (from NCBI database), which expression should be decreased can be provided. Moreover, we expanded functionality of the software with off-target validation and check of immune motifs and also extended miRNA-shuttles database. sh-miR Designer is a software aimed for fast and efficient design of effective RNA interference (RNAi) reagents - sh-miRs, also known as artificial miRNAs. sh-miRs are RNA particles whose structure is based on miRNA precursor pri-miRNA, but sequence interacting with transcript is changed depending on research purpose. Maintenance of structure of pri-miRNA is very important to enable cellular processing and therefore ensure functionality of artificial particles. sh-miRs delivered to cells on genetic vectors - plasmids or viral vectors - enter natural RNAi pathway and silence target mRNA. They can be used in genetic therapies and basic biomedical research. We will provide two applications to access the software, one which require siRNA sequences and the second which require transcript accession number from NCBI database. Each user will receive an account with login (e-mail) and password where he/she would be able to check history of software usage.input form: users' history: Standard PartsWe are creating a particle which can be inserted into plasmid built by Standard parts from Registry of Standard Biological Parts.User GuideUsage via Web server
go to our website shmir.wmi.amu.edu.pl
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