http://2014.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=Mosthege&year=&month=2014.igem.org - User contributions [en]2024-03-29T13:40:04ZFrom 2014.igem.orgMediaWiki 1.16.5http://2014.igem.org/User:MosthegeUser:Mosthege2015-02-19T12:54:03Z<p>Mosthege: /* */</p>
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== ==<br />
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'''Michael Osthege - Computational & Lab Division'''<br />
<br />
''Molecular and Applied Biotechnology (B.Sc. student)''<br />
<br />
As hobby developer and member of the [https://2014.igem.org/Team:Aachen 2014 iGEM Team at RWTH Aachen University], I'm currently exploring synthetic biology.<br />
<br />
Apart from my interests in molecular and synthetic biology, [http://mosthege.net I'm blogging] about programming apps and my recent interest in Arduino microcontrollers. I relax with nice bicycle tours and a round of poker now and then. As an opportunity to learn new things and bring other talents and interests to good use, I enjoyed working with our team at iGEM 2014.<br />
<br />
'''Mainly involved in...'''<br />
* planning of subprojects<br />
* collaborations with other iGEM teams<br />
* writing tools analysis and processing of experiment data<br />
<br />
==Relevant Standalone Software==<br />
<br />
'''Sequence Buddy''' is a free Windows 8 app that is designed to accelerate molecular design workflows. These are the most prominent features:<br />
* calculation of protein mass<br />
* checking a sequence for '''BioBrick compability'''<br />
* reverse / complement of nucleotide sequences<br />
* translation of DNA sequences<br />
* instant results<br />
[[File:Mosthege_WSbadge_154x40.png|link=http://apps.microsoft.com/windows/en-us/app/sequence-buddy/a6de6269-17a1-4a89-a85d-f5d436ec6744]]<br />
<br />
'''Serial Client''' is a Windows desktop application for online-collection of raw data on serial ports. The app is designed and tested for:<br />
* recording of about 200 readouts/second<br />
* convenient export of recorded data to Excel<br />
* live visualization of incoming data<br />
For more information on the Serial Client, please visit our [[Team:Aachen/Notebook/Software|Software Section]].<br />
<br />
'''Timeseries Labeller''' is another Windows Desktop application for batch-labelling of many frames in a timeseries. The app is built for:<br />
* labelling with a short text<br />
* consistent labelling of frames with a time indicator<br />
* convenient export of frames as PNG-files for further processing<br />
For more information on the Timeseries Labeller, please visit our [[Team:Aachen/Notebook/Software|Software Section]].<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T11:07:08Z<p>Mosthege: </p>
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
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<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<table style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<tr><td><div style="font-size:175%;margin-top:12px;">Achievements</div></td></tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">The whole project was awarded <b>Best Measurement Project</b> and won the track.</p></td><br />
<td><img style="margin-bottom:25px;" src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /></td><br />
</tr><br />
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<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">Our image analysis software <i>Measurarty</i> was awarded <b>Best Supporting Software</b> in the overgrad section.</p></td><br />
<td><img style="margin-bottom:25px;" src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">Our work on biosafety was rewarded with the <b>Safety Commendation</b> special award.</p></td><br />
<td><img style="margin-bottom:25px;" src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
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<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">We fulfilled all Bronze and Silver criteria and won a <b>Gold Medal</b>.</p></td><br />
<td><img style="margin-bottom:25px;" src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /></td><br />
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|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
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|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T11:06:35Z<p>Mosthege: </p>
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<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<table style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<tr><td><div style="font-size:175%;margin-top:12px;">Achievements</div></td></tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">The whole project was awarded <b>Best Measurement Project</b> and won the track.</p></td><br />
<td><img style="margin-bottom:20px;" src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">Our image analysis software <i>Measurarty</i> was awarded <b>Best Supporting Software</b> in the overgrad section.</p></td><br />
<td><img style="margin-bottom:20px;" src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">Our work on biosafety was rewarded with the <b>Safety Commendation</b> special award.</p></td><br />
<td><img style="margin-bottom:20px;" src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:20px;overflow:auto;">We fulfilled all Bronze and Silver criteria and won a <b>Gold Medal</b>.</p></td><br />
<td><img style="margin-bottom:20px;" src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /></td><br />
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|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
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|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
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{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T11:04:34Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
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<br/><br />
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<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<table style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<tr><td><div style="font-size:175%;margin-top:12px;">Achievements</div></td></tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">The whole project was awarded <b>Best Measurement Project</b> and won the track.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">Our image analysis software <i>Measurarty</i> was awarded <b>Best Supporting Software</b> in the overgrad section.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">Our work on biosafety was rewarded with the <b>Safety Commendation</b> special award.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">We fulfilled all Bronze and Silver criteria and won a <b>Gold Medal</b>.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /></td><br />
</tr><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="menukachel" style="top:44%;">Collaborations</div><br />
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<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
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</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T11:03:40Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
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<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<table><br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">The whole project was awarded <b>Best Measurement Project</b> and won the track.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">Our image analysis software <i>Measurarty</i> was awarded <b>Best Supporting Software</b> in the overgrad section.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">Our work on biosafety was rewarded with the <b>Safety Commendation</b> special award.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
<tr><br />
<td><p style="margin-left:0px;margin-right:0px;overflow:auto;">We fulfilled all Bronze and Silver criteria and won a <b>Gold Medal</b>.</p></td><br />
<td><img src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /></td><br />
</tr><br />
<br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
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</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:58:49Z<p>Mosthege: </p>
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<table><br />
<tr><br />
<td>Our whole project was awarded <b>Best Measurement Project</b> and won the track.</td><br />
<td><img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /></td><br />
</tr><br />
</table><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
Our whole project was awarded <b>Best Measurement Project</b> and won the track.<br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
</br><br />
Our image analysis software <i>Measurarty</i> was awarded <b>Best Supporting Software</b> in the overgrad section.<br />
<img src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
Our work on biosafety was rewarded with the <b>Safety Commendation</b> special award.<br />
<img src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
We fulfilled all Bronze and Silver criteria and won a <b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /><br />
</p><br />
<br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
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<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:56:32Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
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<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
Our whole project was awarded <b>Best Measurement Project</b> and won the track.<br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
</br><br />
Our image analysis software <i>Measurarty</i> was awarded <b>Best Supporting Software</b> in the overgrad section.<br />
<img src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
Our work on biosafety was rewarded with the <b>Safety Commendation</b> special award.<br />
<img src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
We fulfilled all Bronze and Silver criteria and won a <b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /><br />
</p><br />
<br />
</div><br />
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<!-- NAVIGATION BUTTONS --><br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:52:43Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
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<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Measurement Project</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Supporting Software</b><br />
<img src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Safety Commendation</b><br />
<img src="https://static.igem.org/mediawiki/2014/8/86/Aachen_Icon_Safety.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/2014/5/5d/Aachen_Goldmedal_header.png" width="80px" align="right" /><br />
</p><br />
<br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
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</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
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<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/File:Aachen_Goldmedal_header.pngFile:Aachen Goldmedal header.png2015-02-19T10:52:33Z<p>Mosthege: </p>
<hr />
<div></div>Mosthegehttp://2014.igem.org/File:Aachen_Icon_Safety.pngFile:Aachen Icon Safety.png2015-02-19T10:50:16Z<p>Mosthege: </p>
<hr />
<div></div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:44:14Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Measurement Project</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Supporting Software</b><br />
<img src="https://static.igem.org/mediawiki/2014/1/18/Aachen_SupportingSoftware.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Safety Commendation</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/2014/8/88/Goldmedal_header.png" width="80px" align="right" /><br />
</p><br />
<br />
</div><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/File:Aachen_SupportingSoftware.pngFile:Aachen SupportingSoftware.png2015-02-19T10:43:53Z<p>Mosthege: </p>
<hr />
<div></div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:28:52Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Measurement Project</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Supporting Software</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Safety Commendation</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/2014/8/88/Goldmedal_header.png" width="100px" align="right" /><br />
</p><br />
<br />
</div><br />
<br />
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<!-- NAVIGATION BUTTONS --><br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:27:39Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
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<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Measurement Project</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Best Supporting Software</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Safety Commendation</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;overflow:auto;"><br />
<b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" align="right" /><br />
</p><br />
<br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:22:15Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;"><br />
<b>Best Measurement Project</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="align:right;" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;"><br />
<b>Best Supporting Software</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="align:right;" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;"><br />
<b>Safety Commendation</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="align:right;" /><br />
</p><br />
</br><br />
<br />
<p style="margin-left:0px;margin-right:0px;"><br />
<b>Gold Medal</b><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="align:right;" /><br />
</p><br />
<br />
</div><br />
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<!-- NAVIGATION BUTTONS --><br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
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<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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{{Team:Aachen/BlockSeparatorFront}}<br />
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<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:20:50Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Measurement Project</b><br />
</p><br />
</br><br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Supporting Software</b><br />
</p><br />
</br><br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Safety Commendation</b><br />
</p><br />
</br><br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Gold Medal</b><br />
</p><br />
<br />
</div><br />
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<br />
<!-- NAVIGATION BUTTONS --><br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
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</ul><br />
</html><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
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{{Team:Aachen/BlockSeparatorFront}}<br />
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<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:17:56Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<br />
<div style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Measurement Project</b><br />
</div><br />
<br />
<div style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Supporting Software</b><br />
</div><br />
<br />
<div style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Safety Commendation</b><br />
</div><br />
<br />
<div style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Gold Medal</b><br />
</div><br />
<br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
<ul class="team-grid" style="width:530px;margin-left:20px;float:left"><br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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</ul><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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{{Team:Aachen/BlockSeparatorFront}}<br />
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<center><br />
<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:15:48Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:20px;margin-right:40px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Measurement Project</b><br />
</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Supporting Software</b><br />
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<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Safety Commendation</b><br />
</p><br />
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<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
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|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
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{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:13:10Z<p>Mosthege: </p>
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<div>__NOTOC__<br />
{{CSS/Main}}<br />
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<br/><br />
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" width="80px" style="float:right;" /><br />
<b>Best Measurement Project</b><br />
</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
<ul class="team-grid" style="width:530px;margin-left:20px;float:left"><br />
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<div class="menukachel" style="top:44%;">Project</div><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
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|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
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{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:11:47Z<p>Mosthege: </p>
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{{CSS/Main}}<br />
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p style="margin-left:0px;margin-right:0px;"><br />
<img src="https://static.igem.org/mediawiki/igem.org/0/0a/Icons_measurement.png" /><br />
Best Measurement Project<br />
</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
<ul class="team-grid" style="width:530px;margin-left:20px;float:left"><br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="menukachel" style="top:44%;">Collaborations</div><br />
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|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:10:35Z<p>Mosthege: </p>
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
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<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:right"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
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<div class="menukachel" style="top:44%;">Interlab Study</div><br />
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<div class="menukachel" style="top:44%;">Collaborations</div><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
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{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:09:17Z<p>Mosthege: </p>
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{{CSS/Main}}<br />
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<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
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|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
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{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:09:01Z<p>Mosthege: </p>
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<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
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<div class="menukachel" style="top:44%;">Collaborations</div><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:08:02Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
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<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:175%;margin-top:12px;">Achievements</div ><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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</div><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="menukachel" style="top:44%;">Collaborations</div><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:06:43Z<p>Mosthege: </p>
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{{CSS/Main}}<br />
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<br/><br />
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<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- ACHIEVEMENTS --><br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<div style="font-size:300%;color:#0069b8;">Achievements</div ><br/><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<br />
<!-- NAVIGATION BUTTONS --><br />
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|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:04:40Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
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<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p style="margin-left:0px;margin-right:0px;">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="margin-left:0px;margin-right:0px;">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
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<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:02:50Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
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<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<div style="width:490px;margin-left:40px;margin-right:20px;float:left"><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
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<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:01:30Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
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<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<div><br />
<p style="width:490px;margin-left:40px;margin-right:20px;float:left">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="width:490px;margin-left:40px;margin-right:20px;float:left">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="width:490px;margin-left:40px;margin-right:20px;float:left">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
</div><br />
<br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
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<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T10:00:40Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<p style="width:490px;margin-left:40px;margin-right:20px;float:left">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="width:490px;margin-left:40px;margin-right:20px;float:left">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="width:490px;margin-left:40px;margin-right:20px;float:left">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
<br />
<br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
</html><br />
<br />
<br />
<br />
<br />
<br />
{{Team:Aachen/BlockSeparatorFront}}<br />
<br />
<center><br />
<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T09:58:50Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<html><br />
<br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<p style="width:530px;margin-left:20px;margin-right:20px;float:left">Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p style="width:530px;margin-left:20px;margin-right:20px;float:left">We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p style="width:530px;margin-left:20px;margin-right:20px;float:left">In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
<br />
<br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
</html><br />
<br />
<br />
<br />
<br />
<br />
{{Team:Aachen/BlockSeparatorFront}}<br />
<br />
<center><br />
<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T09:57:57Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
<br />
<html><br />
<p>Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. </p><br />
<br />
<p>We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.</p><br />
<br />
<p>In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.</p><br />
<br />
<br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
</html><br />
<br />
<br />
<br />
<br />
<br />
{{Team:Aachen/BlockSeparatorFront}}<br />
<br />
<center><br />
<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T09:56:41Z<p>Mosthege: </p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/HeaderFront}}<br />
<br />
<!-- [[File:Aachen_Cellock_rand.png|left|250px]] --><br />
<br/><br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. <br />
<br />
We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.<br />
<br />
In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.<br />
<br />
<html><br />
<ul class="team-grid" style="width:530px;margin-left:20px;margin-right:20px;float:left"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Project</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/55/Aachen_14-10-16_Project_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_14-10-16_PolicyPractices_main_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
</ul><br />
</html><br />
<br />
<html><br />
<ul class="team-grid" style="width:1060px;margin-left:20px;margin-right:20px;float:right"><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
<br />
<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/cf/Aachen_main_Team.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
</ul><br />
</html><br />
<br />
<br />
<br />
<br />
<br />
{{Team:Aachen/BlockSeparatorFront}}<br />
<br />
<center><br />
<html><br/><br />
<a href="https://2014.igem.org/Team:Aachen/Team"><img src="https://static.igem.org/mediawiki/2014/d/d2/Aachen_Team_Aachen_Teamfoto_small.jpg" width="1012px" style="border: 3px solid #0069B3; border-radius: 20px; "></a><br />
</html><br />
</center><br />
<br />
{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:AachenTeam:Aachen2015-02-19T09:55:10Z<p>Mosthege: </p>
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<br/><br />
<div style="font-size:300%;color:#0069b8;margin-left:40px;">Cellock Holmes - A Case of Identity</div ><br/><br />
<div style="font-size:175%;margin-top:12px;margin-left:40px;">Welcome to the iGEM Aachen 2014 Wiki!</div ><br />
<br />
Until now, an ideal method to detect pathogenic bacteria is still elusive. The existing techniques require expensive equipment, trained personnel or labourious routines. <br />
<br />
We, the iGEM team Aachen, have developed a '''novel biosensor system''' called ''Cellock Holmes'' that '''detects bacteria on solid surfaces''' using a low-cost, rapid and portable technique.<br />
<br />
In this project, we demonstrate a unique way of combining '''Open Source biology, software and hardware'''.<br />
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<a href="https://2014.igem.org/Team:Aachen/Project" style="color:black"><br />
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<div class="menukachel" style="top:44%;">Project</div><br />
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<a href="https://2014.igem.org/Team:Aachen/PolicyPractices" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;line-height: 2em;" ><br />
<div class="menukachel">Policy & Practices</div><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Interlab_Study" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Interlab Study</div><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
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<a href="https://2014.igem.org/Team:Aachen/Collaborations" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Collaborations</div><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_14-10-15_Collaborating_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Notebook" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Notebook</div><br />
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<b>Principle of Operation</br><br />
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click for more information --><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/42/Aachen_14-10-14_NotebookiFG.png); norepeat scroll 0% 0% transparent; background-size:100%;width:219px;height:219px;"> </div></a><br />
</li><br />
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<li style="width:225px;margin-left: 20px;margin-right: 20px;margin-bottom: 30px;margin-top: 10px;"><br />
<a href="https://2014.igem.org/Team:Aachen/Attributions" style="color:black"><br />
<div class="team-item team-info" style="width:219px;height:219px;" ><br />
<div class="menukachel" style="top:44%;">Team</div><br />
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{|cellpadding="12"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|80px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|159px|link=http://www.niersverband.de/|Niersverband]]<br />
|[[File:Aachen_Genscript_Logo.png|120px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|97px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|128px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
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{|cellpadding="18"<br />
|[[File:Aachen_bmbf.jpg|163px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|109px|link=http://eu.idtdna.com/site|IDT]]<br />
|[[File:M2p_labs_logo.jpg|52px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|55px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|62px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Logo_iAMB.png|115px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|115px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|123px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|[[File:Aachen_Logo_ABBt.png|105px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|120px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|105px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|75px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
{{Team:Aachen/FooterFront}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/TestBench3Team:Aachen/TestBench32014-12-11T14:35:26Z<p>Mosthege: Undo revision 403785 by Mosthege (talk)</p>
<hr />
<div>=Dummy Content=<br />
Wonderful woman. Very free-spirited. We're all very fond of her. And I'm talkin' about the Dude here —sometimes there's a man who, wal, he's the man for his time'n place, he fits right in there—and that's the Dude, in Los Angeles. They call Los Angeles the City of Angels. I didn't find it to be that exactly, but I'll allow as there are some nice folks there. 'Course, I can't say I seen London, and I never been to France, and I ain't never seen no queen in her damn undies as the fella says. But I'll tell you what, after seeing Los Angeles and thisahere story I'm about to unfold —wal, I guess I seen somethin' ever' bit as stupefyin' as ya'd see in any a those other places, and in English too, so I can die with a smile on my face without feelin' like the good Lord gypped me.<br />
<br />
They won't hurt us, Donny. These men are cowards. Fine, Dude. As if it's impossible to get some nail polish, apply it to someone else's toe. Donny was a good bowler, and a good man. He was… He was one of us. He was a man who loved the outdoors, and bowling, and as a surfer explored the beaches of southern California from Redondo to Calabassos. And he was an avid bowler. And a good friend. He died—he died as so many of his generation, before his time. In your wisdom you took him, Lord. As you took so many bright flowering young men, at Khe San and Lan Doc.<br />
<br />
Look, I've got certain information, certain things have come to light, and uh, has it ever occurred to you, man, that given the nature of all this new shit, that, uh, instead of running around blaming me, that this whole thing might just be, not, you know, not just such a simple, but uh—you know? Your goons'll be able to get it off him, mean he's only fifteen and he's flunking social studies. So if you'll just write me a check for my ten per cent… of half a million… fifty grand.<br />
<br />
Whose toe was it, Walter? Is this yours, Larry? Is this your homework, Larry? Your "revolution" is over, Mr. Lebowski! Condolences! The bums lost! Hello, Pilar? My name is Walter Sobchak, we spoke on the phone, this is my associate Jeffrey Lebowski. Blow on them. Strong men also cry… Strong men also cry. Our basic freedoms. Uh, yeah. Probably a vagrant, slept in the car. Or perhaps just used it as a toilet, and moved on.<br />
<br />
Fuckin' A. No ma'am, I didn't mean to give the impression that we're police exactly. We're hoping that it will not be necessary to call the police. Hey, man, if my fucking ex-wife asked me to take care of her fucking dog while she and her boyfriend went to Honolulu, I'd tell her to go fuck herself. They finally did it. They killed my fucking car.</div>Mosthegehttp://2014.igem.org/Team:Aachen/TestBench3Team:Aachen/TestBench32014-12-11T14:31:19Z<p>Mosthege: </p>
<hr />
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<h1>Dummy Content</h1><br />
<p><br />
Wonderful woman. Very free-spirited. We're all very fond of her. And I'm talkin' about the Dude here —sometimes there's a man who, wal, he's the man for his time'n place, he fits right in there—and that's the Dude, in Los Angeles. They call Los Angeles the City of Angels. I didn't find it to be that exactly, but I'll allow as there are some nice folks there. 'Course, I can't say I seen London, and I never been to France, and I ain't never seen no queen in her damn undies as the fella says. But I'll tell you what, after seeing Los Angeles and thisahere story I'm about to unfold —wal, I guess I seen somethin' ever' bit as stupefyin' as ya'd see in any a those other places, and in English too, so I can die with a smile on my face without feelin' like the good Lord gypped me.<br />
<br />
They won't hurt us, Donny. These men are cowards. Fine, Dude. As if it's impossible to get some nail polish, apply it to someone else's toe. Donny was a good bowler, and a good man. He was… He was one of us. He was a man who loved the outdoors, and bowling, and as a surfer explored the beaches of southern California from Redondo to Calabassos. And he was an avid bowler. And a good friend. He died—he died as so many of his generation, before his time. In your wisdom you took him, Lord. As you took so many bright flowering young men, at Khe San and Lan Doc.<br />
<br />
Look, I've got certain information, certain things have come to light, and uh, has it ever occurred to you, man, that given the nature of all this new shit, that, uh, instead of running around blaming me, that this whole thing might just be, not, you know, not just such a simple, but uh—you know? Your goons'll be able to get it off him, mean he's only fifteen and he's flunking social studies. So if you'll just write me a check for my ten per cent… of half a million… fifty grand.<br />
<br />
Whose toe was it, Walter? Is this yours, Larry? Is this your homework, Larry? Your "revolution" is over, Mr. Lebowski! Condolences! The bums lost! Hello, Pilar? My name is Walter Sobchak, we spoke on the phone, this is my associate Jeffrey Lebowski. Blow on them. Strong men also cry… Strong men also cry. Our basic freedoms. Uh, yeah. Probably a vagrant, slept in the car. Or perhaps just used it as a toilet, and moved on.<br />
<br />
Fuckin' A. No ma'am, I didn't mean to give the impression that we're police exactly. We're hoping that it will not be necessary to call the police. Hey, man, if my fucking ex-wife asked me to take care of her fucking dog while she and her boyfriend went to Honolulu, I'd tell her to go fuck herself. They finally did it. They killed my fucking car.<br />
</p><br />
</body><br />
</html></div>Mosthegehttp://2014.igem.org/Team:Aachen/TestBench3Team:Aachen/TestBench32014-12-04T10:33:46Z<p>Mosthege: Created page with "=Dummy Content= Wonderful woman. Very free-spirited. We're all very fond of her. And I'm talkin' about the Dude here —sometimes there's a man who, wal, he's the man for his tim..."</p>
<hr />
<div>=Dummy Content=<br />
Wonderful woman. Very free-spirited. We're all very fond of her. And I'm talkin' about the Dude here —sometimes there's a man who, wal, he's the man for his time'n place, he fits right in there—and that's the Dude, in Los Angeles. They call Los Angeles the City of Angels. I didn't find it to be that exactly, but I'll allow as there are some nice folks there. 'Course, I can't say I seen London, and I never been to France, and I ain't never seen no queen in her damn undies as the fella says. But I'll tell you what, after seeing Los Angeles and thisahere story I'm about to unfold —wal, I guess I seen somethin' ever' bit as stupefyin' as ya'd see in any a those other places, and in English too, so I can die with a smile on my face without feelin' like the good Lord gypped me.<br />
<br />
They won't hurt us, Donny. These men are cowards. Fine, Dude. As if it's impossible to get some nail polish, apply it to someone else's toe. Donny was a good bowler, and a good man. He was… He was one of us. He was a man who loved the outdoors, and bowling, and as a surfer explored the beaches of southern California from Redondo to Calabassos. And he was an avid bowler. And a good friend. He died—he died as so many of his generation, before his time. In your wisdom you took him, Lord. As you took so many bright flowering young men, at Khe San and Lan Doc.<br />
<br />
Look, I've got certain information, certain things have come to light, and uh, has it ever occurred to you, man, that given the nature of all this new shit, that, uh, instead of running around blaming me, that this whole thing might just be, not, you know, not just such a simple, but uh—you know? Your goons'll be able to get it off him, mean he's only fifteen and he's flunking social studies. So if you'll just write me a check for my ten per cent… of half a million… fifty grand.<br />
<br />
Whose toe was it, Walter? Is this yours, Larry? Is this your homework, Larry? Your "revolution" is over, Mr. Lebowski! Condolences! The bums lost! Hello, Pilar? My name is Walter Sobchak, we spoke on the phone, this is my associate Jeffrey Lebowski. Blow on them. Strong men also cry… Strong men also cry. Our basic freedoms. Uh, yeah. Probably a vagrant, slept in the car. Or perhaps just used it as a toilet, and moved on.<br />
<br />
Fuckin' A. No ma'am, I didn't mean to give the impression that we're police exactly. We're hoping that it will not be necessary to call the police. Hey, man, if my fucking ex-wife asked me to take care of her fucking dog while she and her boyfriend went to Honolulu, I'd tell her to go fuck herself. They finally did it. They killed my fucking car.</div>Mosthegehttp://2014.igem.org/Team:Aachen/AchievementsTeam:Aachen/Achievements2014-10-18T03:55:09Z<p>Mosthege: /* Medal Fulfillments */</p>
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=Achievements=<br />
<br />
On this page, we summarized all things we accomplished throughout our project "''Cellock Holmes'' - A Case of Identity", and present the medal criteria we have fulfilled for the iGEM competition 2014:<br />
<br />
<html><br />
<center><br />
<ul class="menusmall-grid" style="width:1040px"><br />
<!-- Overview --><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achmedals" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Medal Fulfillments</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/2/2d/Aachen_14-10-14_Medals_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
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<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achbiosensor" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">Biosensor</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/5a/Aachen_14-10-14_cellock_liegend_panel_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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</a><br />
</li><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achwatson" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;"><i>WatsOn</i></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c7/Aachen_WatsOn_easy.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achdevice" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">OD/F Device</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0f/Aachen_14-10-10_ODF_Button_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achinterlab" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Interlab<br>Study</br></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
</ul><br />
</center><br />
</html><br />
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{{Team:Aachen/BlockSeparator}}<br />
<br />
==Medal Fulfillments==<br />
<span class="anchor" id="achmedals"></span><br />
<br />
{| style="float:left; margin-left:30px; margin-bottom:20px"<br />
| https://static.igem.org/mediawiki/2014/thumb/b/b8/Aachen_14-10-14_Bronze_iFG.png/230px-Aachen_14-10-14_Bronze_iFG.png<br />
|<span style="font-size:150%">'''Bronze Requirements (6/6)'''</span><br />
# Our team was registered successfully.<br />
# The [https://igem.org/2014_Judging_Form?id=1319 Judging form] was completed.<br />
# Team [https://2014.igem.org/Team:Aachen/ Wiki] was created.<br />
# We are looking forward to present a poster and a talk at the iGEM Jamboree.<br />
# We successfully participated in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study].<br />
# Several [https://2014.igem.org/Team:Aachen/Parts parts] were submitted to the [http://parts.igem.org/Main_Page Registry of Standard Biological Parts].<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/4/42/Aachen_14-10-14_Silver_iFG.png/230px-Aachen_14-10-14_Silver_iFG.png<br />
|<span style="font-size:150%;">'''Silver Requirements (4/4)</span><br />
# It was experimentally validated that [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] and Devices of our own design work as expected.<br />
# The characterization of [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] is documented in the 'Main Page' section of that Registry entry and in the parts section of our Wiki.<br />
# [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] were submitted to the iGEM Parts Registry.<br />
# During the project we addressed several [https://2014.igem.org/Team:Aachen/PolicyPractices important questions beyond the bench]. Together with students of the [https://2014.igem.org/Team:Aachen/Collaborations/Kaiser-Karls-Gymnasium Kaiser-Karls-Gymnasium] and the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab Gymnasiun am Neandertal] as well as people at the [https://2014.igem.org/Team:Aachen/Collaborations/MakerFaire MakerFaire] and at the [https://2014.igem.org/Team:Aachen/Meetup iGEM Meetup] we discussed several issues regarding our project, microorganisms in our environment and synthetic biology. Since we use genetically engineered bacteria to detect human pathogens, [https://2014.igem.org/Team:Aachen/Safety safety] was an important aspect for us throughout the whole project. Together with other German iGEM Teams we encountered an issue regarding [https://2014.igem.org/Team:Aachen/PolicyPractices/BioBrickIntellectualProperty intellectual property rights] in context of the legal status of Biobricks.<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/5/5b/Aachen_14-10-14_Gold_iFG.png/230px-Aachen_14-10-14_Gold_iFG.png<br />
|<span style="font-size:150%;">'''Gold Requirements (4/1)'''</span><br />
# We demonstrated a substantial improvement in cost efficiency of our [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] in comparison to commercially available spectrophotometers. For more information see [https://2014.igem.org/Team:Aachen/PolicyPractices/Economics Economical View].<br />
# We increased the accessability of our new measurement techniques by sharing instructions to build our DIY devices [https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn#watsondiy ''WatsOn''] and the [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy OD/F Device].<br />
# Helped three other iGEM Teams:<br />
#:* We built a [https://2014.igem.org/Team:Aachen/Collaborations/Braunschweig low-cost DIY methane sensor] for [https://2014.igem.org/Team:Braunschweig Team Braunschweig].<br />
#:* We built [https://2014.igem.org/Team:Aachen/Collaborations/Freiburg AcCELLoMatrix Masks] for [https://2014.igem.org/Team:Freiburg Team Freiburg].<br />
#:* We [https://2014.igem.org/Team:Aachen/Collaborations/Heidelberg tested different expression vectors] constructed by [https://2014.igem.org/Team:Heidelberg Team Heidelberg].<br />
# On top of the Policy & Practice aspects already mentioned in the Silver Medal Requirements, we also critically [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab#neanderlabevaluation evaluated] the effectiveness of our approach to increase the social acceptance of gene technology in general, and of our initiative to spark interest for synthetic biology and DIY hardware.<br />
|}<br />
<br />
In addition to the medal criteria of the measurement track, we have characterized the existing BioBricks [http://parts.igem.org/Part:BBa_K131026:Experience K131026] and [http://parts.igem.org/Part:BBa_I746909:Experience I746909]. We improved [http://parts.igem.org/Part:BBa_K731520 K731520] by replacing the GFP with an iLOV (now [http://parts.igem.org/Part:BBa_K1319042 K1319042]) and showed that it functions in our two-dimensional biosensor ([[Team:Aachen/Project/2D_Biosensor#Achievements|link]]).<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-14_cellock_liegend_panel_iNB.png|right|150px]]<br />
<br />
==Biosensor==<br />
<span class="anchor" id="achbiosensor"></span><br />
<br />
We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] for detecting pathogens using a two dimensional biosensor. In our approach, the sensor cells are immobilized in '''[https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensordevelopment optimized agar chips]'''. It has been '''experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated]''' that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''].<br />
<br />
In order to improve the response of our sensor cells to induction we '''designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system]''' using a TEV protease to activate a reporter protein by cleaving off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by '''[https://2014.igem.org/Team:Aachen/Project/Model computational modeling] as well as in our [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements experiments]'''. All parts needed to build this kind of reporter system have been '''submitted to the parts registry'''.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen WatsOn easy.png|right|150px]]<br />
<br />
==''WatsOn''==<br />
<span class="anchor" id="achwatson"></span><br />
<br />
[https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] is a measuring device designed to read out novel two dimensional biosensor. Using ''WatsOn'', we could demonstrate the '''successful detection''' of ''Pseudomonas&nbsp;aeruginosa'' with our chip technology. This measurement device is built from inexpensive and easily available parts to make it ideal for use in low-budget institutions. Following our economic strategy embracing the Open Access principle, we published all technical details and constructional manuals for ''WatsOn'' on our wiki.<br />
<br />
In addition, we have shown that our '''image analysis software [https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonmeasurarty ''Measurarty'']''' can analyze photos of the sensor chips taken by ''WatsOn'' and can '''effectively segment the image''' into pathogenic regions and non-pathogenic regions.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-10_ODF_Button_ipo.png|right|150px]]<br />
<br />
==OD/F Device==<br />
<span class="anchor" id="achdevice"></span><br />
<br />
In addition to our main project, we built an [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] capable of measuring optical density and flourescence. It '''beats commercially available devices in cost and portability'''. It can be built with common, inexpensive and easily available parts. A [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy construction manual] is available on our wiki.<br />
<br />
The device has been '''successfully tested''' with one of the target groups, high school students, in our collaboration with the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab NEAnderLab].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_Interlab_Cellocks.png|right|150px]]<br />
<br />
==Interlab Study==<br />
<span class="anchor" id="achinterlab"></span><br />
<br />
Since we compete in the Measurement Track of this year's iGEM competition, one of our bronze medal criteria was to participate in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study]. Our team was able to measure optical density and fluorescence of ''E.&nbsp;coli'' cells containing the three specified genetic devices, and '''obtained high quality data''' supporting the hypotheses regarding different expression levels depending on plamid copy number and promoter strength.<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/AchievementsTeam:Aachen/Achievements2014-10-18T03:52:40Z<p>Mosthege: /* Medal Fulfillments */</p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/Header}}<br />
<br />
=Achievements=<br />
<br />
On this page, we summarized all things we accomplished throughout our project "''Cellock Holmes'' - A Case of Identity", and present the medal criteria we have fulfilled for the iGEM competition 2014:<br />
<br />
<html><br />
<center><br />
<ul class="menusmall-grid" style="width:1040px"><br />
<!-- Overview --><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achmedals" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Medal Fulfillments</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/2/2d/Aachen_14-10-14_Medals_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achbiosensor" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">Biosensor</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/5a/Aachen_14-10-14_cellock_liegend_panel_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achwatson" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;"><i>WatsOn</i></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c7/Aachen_WatsOn_easy.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achdevice" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">OD/F Device</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0f/Aachen_14-10-10_ODF_Button_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achinterlab" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Interlab<br>Study</br></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
</ul><br />
</center><br />
</html><br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==Medal Fulfillments==<br />
<span class="anchor" id="achmedals"></span><br />
<br />
{| style="float:left; margin-left:30px; margin-bottom:20px"<br />
| https://static.igem.org/mediawiki/2014/thumb/b/b8/Aachen_14-10-14_Bronze_iFG.png/230px-Aachen_14-10-14_Bronze_iFG.png<br />
|<span style="font-size:150%">'''Bronze Requirements (6/6)'''</span><br />
# Our team was registered successfully.<br />
# The [https://igem.org/2014_Judging_Form?id=1319 Judging form] was completed.<br />
# Team [https://2014.igem.org/Team:Aachen/ Wiki] was created.<br />
# We are looking forward to present a poster and a talk at the iGEM Jamboree.<br />
# We successfully participated in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study].<br />
# Several [https://2014.igem.org/Team:Aachen/Parts parts] were submitted to the [http://parts.igem.org/Main_Page Registry of Standard Biological Parts].<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/4/42/Aachen_14-10-14_Silver_iFG.png/230px-Aachen_14-10-14_Silver_iFG.png<br />
|<span style="font-size:150%;">'''Silver Requirements (4/4)</span><br />
# It was experimentally validated that [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] and Devices of our own design work as expected.<br />
# The characterization of [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] is documented in the 'Main Page' section of that Registry entry and in the parts section of our Wiki.<br />
# [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] were submitted to the iGEM Parts Registry.<br />
# During the project we addressed several [https://2014.igem.org/Team:Aachen/PolicyPractices important questions beyond the bench]. Together with students of the [https://2014.igem.org/Team:Aachen/Collaborations/Kaiser-Karls-Gymnasium Kaiser-Karls-Gymnasium] and the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab Gymnasiun am Neandertal] as well as people at the [https://2014.igem.org/Team:Aachen/Collaborations/MakerFaire MakerFaire] and at the [https://2014.igem.org/Team:Aachen/Meetup iGEM Meetup] we discussed several issues regarding our project, microorganisms in our environment and synthetic biology. Since we use genetically engineered bacteria to detect human pathogens, [https://2014.igem.org/Team:Aachen/Safety safety] was an important aspect for us throughout the whole project. Together with other German iGEM Teams we encountered an issue regarding [https://2014.igem.org/Team:Aachen/PolicyPractices/BioBrickIntellectualProperty intellectual property rights] in context of the legal status of Biobricks.<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/5/5b/Aachen_14-10-14_Gold_iFG.png/230px-Aachen_14-10-14_Gold_iFG.png<br />
|<span style="font-size:150%;">'''Gold Requirements (4/1)'''</span><br />
# We demonstrated a substantial improvement in cost efficiency of our [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] in comparison to commercially available spectrophotometers. For more information see [https://2014.igem.org/Team:Aachen/PolicyPractices/Economics Economical View].<br />
# We increased the accessability of our new measurement techniques by sharing instructions to build our DIY devices [https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn#watsondiy ''WatsOn''] and the [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy OD/F Device].<br />
# Helped three other iGEM Teams:<br />
#:* We built a [https://2014.igem.org/Team:Aachen/Collaborations/Braunschweig low-cost DIY methane sensor] for [https://2014.igem.org/Team:Braunschweig Team Braunschweig].<br />
#:* We built [https://2014.igem.org/Team:Aachen/Collaborations/Freiburg AcCELLoMatrix Masks] for [https://2014.igem.org/Team:Freiburg Team Freiburg].<br />
#:* We [https://2014.igem.org/Team:Aachen/Collaborations/Heidelberg tested different expression vectors] constructed by [https://2014.igem.org/Team:Heidelberg Team Heidelberg].<br />
# On top of the Policy & Practice aspects already mentioned in the Silver Medal Requirements, we also critically [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab#neanderlabevaluation evaluated] the effectiveness of our approach to increase the social acceptance of gene technology in general, and of our initiative to spark interest for synthetic biology and DIY hardware.<br />
|}<br />
<br />
In addition to the medal criteria of the measurement track, we have characterized the existing BioBricks [http://parts.igem.org/Part:BBa_K131026:Experience K131026] and [http://parts.igem.org/Part:BBa_I746909:Experience I746909]. We improved [http://parts.igem.org/Part:BBa_K731520 K731520] by replacing the GFP with an iLOV (now [http://parts.igem.org/Part:BBa_K1319042 K1319042]) and showed that it functions in our two-dimensional biosensor [[Team:Aachen/Project/2D_Biosensor#Achievements|link]].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-14_cellock_liegend_panel_iNB.png|right|150px]]<br />
<br />
==Biosensor==<br />
<span class="anchor" id="achbiosensor"></span><br />
<br />
We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] for detecting pathogens using a two dimensional biosensor. In our approach, the sensor cells are immobilized in '''[https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensordevelopment optimized agar chips]'''. It has been '''experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated]''' that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''].<br />
<br />
In order to improve the response of our sensor cells to induction we '''designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system]''' using a TEV protease to activate a reporter protein by cleaving off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by '''[https://2014.igem.org/Team:Aachen/Project/Model computational modeling] as well as in our [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements experiments]'''. All parts needed to build this kind of reporter system have been '''submitted to the parts registry'''.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen WatsOn easy.png|right|150px]]<br />
<br />
==''WatsOn''==<br />
<span class="anchor" id="achwatson"></span><br />
<br />
[https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] is a measuring device designed to read out novel two dimensional biosensor. Using ''WatsOn'', we could demonstrate the '''successful detection''' of ''Pseudomonas&nbsp;aeruginosa'' with our chip technology. This measurement device is built from inexpensive and easily available parts to make it ideal for use in low-budget institutions. Following our economic strategy embracing the Open Access principle, we published all technical details and constructional manuals for ''WatsOn'' on our wiki.<br />
<br />
In addition, we have shown that our '''image analysis software [https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonmeasurarty ''Measurarty'']''' can analyze photos of the sensor chips taken by ''WatsOn'' and can '''effectively segment the image''' into pathogenic regions and non-pathogenic regions.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-10_ODF_Button_ipo.png|right|150px]]<br />
<br />
==OD/F Device==<br />
<span class="anchor" id="achdevice"></span><br />
<br />
In addition to our main project, we built an [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] capable of measuring optical density and flourescence. It '''beats commercially available devices in cost and portability'''. It can be built with common, inexpensive and easily available parts. A [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy construction manual] is available on our wiki.<br />
<br />
The device has been '''successfully tested''' with one of the target groups, high school students, in our collaboration with the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab NEAnderLab].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_Interlab_Cellocks.png|right|150px]]<br />
<br />
==Interlab Study==<br />
<span class="anchor" id="achinterlab"></span><br />
<br />
Since we compete in the Measurement Track of this year's iGEM competition, one of our bronze medal criteria was to participate in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study]. Our team was able to measure optical density and fluorescence of ''E.&nbsp;coli'' cells containing the three specified genetic devices, and '''obtained high quality data''' supporting the hypotheses regarding different expression levels depending on plamid copy number and promoter strength.<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/AchievementsTeam:Aachen/Achievements2014-10-18T03:49:12Z<p>Mosthege: /* Medal Fulfillments */</p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/Header}}<br />
<br />
=Achievements=<br />
<br />
On this page, we summarized all things we accomplished throughout our project "''Cellock Holmes'' - A Case of Identity", and present the medal criteria we have fulfilled for the iGEM competition 2014:<br />
<br />
<html><br />
<center><br />
<ul class="menusmall-grid" style="width:1040px"><br />
<!-- Overview --><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achmedals" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Medal Fulfillments</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/2/2d/Aachen_14-10-14_Medals_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achbiosensor" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">Biosensor</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/5a/Aachen_14-10-14_cellock_liegend_panel_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achwatson" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;"><i>WatsOn</i></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c7/Aachen_WatsOn_easy.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achdevice" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">OD/F Device</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0f/Aachen_14-10-10_ODF_Button_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achinterlab" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Interlab<br>Study</br></div></div><br />
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<br />
==Medal Fulfillments==<br />
<span class="anchor" id="achmedals"></span><br />
<br />
{| style="float:left; margin-left:30px; margin-bottom:20px"<br />
| https://static.igem.org/mediawiki/2014/thumb/b/b8/Aachen_14-10-14_Bronze_iFG.png/230px-Aachen_14-10-14_Bronze_iFG.png<br />
|<span style="font-size:150%">'''Bronze Requirements (6/6)'''</span><br />
# Our team was registered successfully.<br />
# The [https://igem.org/2014_Judging_Form?id=1319 Judging form] was completed.<br />
# Team [https://2014.igem.org/Team:Aachen/ Wiki] was created.<br />
# We are looking forward to present a poster and a talk at the iGEM Jamboree.<br />
# We successfully participated in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study].<br />
# Several [https://2014.igem.org/Team:Aachen/Parts parts] were submitted to the [http://parts.igem.org/Main_Page Registry of Standard Biological Parts].<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/4/42/Aachen_14-10-14_Silver_iFG.png/230px-Aachen_14-10-14_Silver_iFG.png<br />
|<span style="font-size:150%;">'''Silver Requirements (4/4)</span><br />
# It was experimentally validated that [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] and Devices of our own design work as expected.<br />
# The characterization of [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] is documented in the 'Main Page' section of that Registry entry and in the parts section of our Wiki.<br />
# [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] were submitted to the iGEM Parts Registry.<br />
# During the project we addressed several [https://2014.igem.org/Team:Aachen/PolicyPractices important questions beyond the bench]. Together with students of the [https://2014.igem.org/Team:Aachen/Collaborations/Kaiser-Karls-Gymnasium Kaiser-Karls-Gymnasium] and the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab Gymnasiun am Neandertal] as well as people at the [https://2014.igem.org/Team:Aachen/Collaborations/MakerFaire MakerFaire] and at the [https://2014.igem.org/Team:Aachen/Meetup iGEM Meetup] we discussed several issues regarding our project, microorganisms in our environment and synthetic biology. Since we use genetically engineered bacteria to detect human pathogens, [https://2014.igem.org/Team:Aachen/Safety safety] was an important aspect for us throughout the whole project. Together with other German iGEM Teams we encountered an issue regarding [https://2014.igem.org/Team:Aachen/PolicyPractices/BioBrickIntellectualProperty intellectual property rights] in context of the legal status of Biobricks.<br />
|-<br />
|colspan="2" | &nbsp;<br />
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|https://static.igem.org/mediawiki/2014/thumb/5/5b/Aachen_14-10-14_Gold_iFG.png/230px-Aachen_14-10-14_Gold_iFG.png<br />
|<span style="font-size:150%;">'''Gold Requirements (4/1)'''</span><br />
# We demonstrated a substantial improvement in cost efficiency of our [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] in comparison to commercially available spectrophotometers. For more information see [https://2014.igem.org/Team:Aachen/PolicyPractices/Economics Economical View].<br />
# We increased the accessability of our new measurement techniques by sharing instructions to build our DIY devices [https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn#watsondiy ''WatsOn''] and the [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy OD/F Device].<br />
# Helped three other iGEM Teams:<br />
#:* We built a [https://2014.igem.org/Team:Aachen/Collaborations/Braunschweig low-cost DIY methane sensor] for [https://2014.igem.org/Team:Braunschweig Team Braunschweig].<br />
#:* We built [https://2014.igem.org/Team:Aachen/Collaborations/Freiburg AcCELLoMatrix Masks] for [https://2014.igem.org/Team:Freiburg Team Freiburg].<br />
#:* We [https://2014.igem.org/Team:Aachen/Collaborations/Heidelberg tested different expression vectors] constructed by [https://2014.igem.org/Team:Heidelberg Team Heidelberg].<br />
# On top of the Policy & Practice aspects already mentioned in the Silver Medal Requirements, we also critically [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab#neanderlabevaluation evaluated] the effectiveness of our approach to increase the social acceptance of gene technology in general, and of our initiative to spark interest for synthetic biology and DIY hardware.<br />
|}<br />
<br />
In addition to the medal criteria of the measurement track, we have characterized the existing BioBricks [http://parts.igem.org/Part:BBa_K131026:Experience K131026] and [http://parts.igem.org/Part:BBa_I746909:Experience I746909]. We improved [http://parts.igem.org/Part:BBa_K731520 K731520] by swapping the GFP with an iLOV (now [http://parts.igem.org/Part:BBa_K1319042 K1319042]) and showed that it functions in our two-dimensional biosensor [[Team:Aachen/Project/2D_Biosensor#Achievements|link]].<br />
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[[File:Aachen_14-10-14_cellock_liegend_panel_iNB.png|right|150px]]<br />
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==Biosensor==<br />
<span class="anchor" id="achbiosensor"></span><br />
<br />
We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] for detecting pathogens using a two dimensional biosensor. In our approach, the sensor cells are immobilized in '''[https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensordevelopment optimized agar chips]'''. It has been '''experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated]''' that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''].<br />
<br />
In order to improve the response of our sensor cells to induction we '''designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system]''' using a TEV protease to activate a reporter protein by cleaving off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by '''[https://2014.igem.org/Team:Aachen/Project/Model computational modeling] as well as in our [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements experiments]'''. All parts needed to build this kind of reporter system have been '''submitted to the parts registry'''.<br />
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[[File:Aachen WatsOn easy.png|right|150px]]<br />
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==''WatsOn''==<br />
<span class="anchor" id="achwatson"></span><br />
<br />
[https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] is a measuring device designed to read out novel two dimensional biosensor. Using ''WatsOn'', we could demonstrate the '''successful detection''' of ''Pseudomonas&nbsp;aeruginosa'' with our chip technology. This measurement device is built from inexpensive and easily available parts to make it ideal for use in low-budget institutions. Following our economic strategy embracing the Open Access principle, we published all technical details and constructional manuals for ''WatsOn'' on our wiki.<br />
<br />
In addition, we have shown that our '''image analysis software [https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonmeasurarty ''Measurarty'']''' can analyze photos of the sensor chips taken by ''WatsOn'' and can '''effectively segment the image''' into pathogenic regions and non-pathogenic regions.<br />
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[[File:Aachen_14-10-10_ODF_Button_ipo.png|right|150px]]<br />
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==OD/F Device==<br />
<span class="anchor" id="achdevice"></span><br />
<br />
In addition to our main project, we built an [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] capable of measuring optical density and flourescence. It '''beats commercially available devices in cost and portability'''. It can be built with common, inexpensive and easily available parts. A [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy construction manual] is available on our wiki.<br />
<br />
The device has been '''successfully tested''' with one of the target groups, high school students, in our collaboration with the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab NEAnderLab].<br />
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[[File:Aachen_Interlab_Cellocks.png|right|150px]]<br />
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==Interlab Study==<br />
<span class="anchor" id="achinterlab"></span><br />
<br />
Since we compete in the Measurement Track of this year's iGEM competition, one of our bronze medal criteria was to participate in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study]. Our team was able to measure optical density and fluorescence of ''E.&nbsp;coli'' cells containing the three specified genetic devices, and '''obtained high quality data''' supporting the hypotheses regarding different expression levels depending on plamid copy number and promoter strength.<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Project/FRET_ReporterTeam:Aachen/Project/FRET Reporter2014-10-18T03:47:18Z<p>Mosthege: /* Comparing the kinetic of the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 with standard GFP expression */</p>
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<div>__NOTOC__<br />
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=The modified REACh Construct=<br />
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On this page, we present our biosensor on a molecular level. Here you can explore the different parts of our genetic device:<br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fluorescence" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">A Faster Answer</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0b/Aachen_14-10-13_GFP_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fret" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">The FRET System</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/54/Aachen_14-10-13_FRET_Arrows_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#darkquencher" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">REACh Quenchers</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#gfp-reach" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">The Fusion Protein</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#tevprotease" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">TEV Protease</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/dc/Aachen_14-10-13_TEV_Protease_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">Achieve-<br/>ments</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/e/ef/Aachen_14-10-15_Medal_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachoutlook" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:40%;">Outlook</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/67/Aachen_14-10-16_Outlook_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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[[File:Aachen_14-10-13_GFP_iNB.png|150px|right]]<br />
<br />
= A Fluorescence Answer Faster than Expression =<br />
<span class="anchor" id="fluorescence"></span><br />
<br />
Biosensor systems are usually based on a reporter gene under the control of a promoter directly induced by the biomolecule of interest. In the case of our 2D biosensor for ''Pseudomonas&nbsp;aeruginosa'', the expression of our fluorescing reporter protein is directly induced by the activity of the bacterium's quorum sensing molecules. However, transcription, translation, folding and post-translational modifications take their time. Since our goal is to detect the pathogen as fast as possible, we aimed to develop a system that provides a fluorescent answer faster than just expressing the fluorescent protein GFP.<br />
<br />
<center><br />
{{Team:Aachen/Figure|Aachen_Traditional_biosensor.png|align=center|title=Schematic model of a traditional biosensor|subtitle=In this model, the expression of GFP is directly controlled by a promoter whose activator binds to a molecule secreted by the pathogen.|width=500px}}<br />
</center><br />
<br />
As an alternative to the traditional approach, we '''constitutively express our reporter gene in a quenched form'''. By fusing GFP to REACh, a mutated variant of EYFP, its original fluorescence is thoroughly suppressed. Thereby, a huge stock of temporary inactivated fluorescence proteins is already available prior to first contact with the pathogen. As a conseqence to the uptake of homoserine lactones of ''P.&nbsp;aeruginosa'' and binding to the LasI promoter in front of the protease gene, these '''autoinducers activate the expression of the TEV protease'''. This way, our biosensor provides a fast increase in fluorescence intensity by cleavage of GFP-REACh-constructs.<br />
<br />
<center><br />
{{Team:Aachen/Figure|Aachen_REACh_approach.png|align=center|title=Schematic model of our novel biosensor|subtitle=Expression of the TEV protease is induced by HSL. The protease cleaves the GFP-REACh fusion protein to elicit a fluorescence response.|width=900px}}<br />
</center><br />
<br />
'''Advantages of the novel biosensor:'''<br />
<br />
* When ''P.&nbsp;aeruginosa'' is detected by our cells, the reporter protein has already been expressed and only waits to be activated. The cleavage reaction catalyzed by the TEV protease is a faster process than expression and correct folding of GFP. Therefore, our sensor cells provide an exceptionally '''early response'''.<br />
<br />
* While a certain concentration of homoserine lactone will produce the same number of gene read-outs and the TEV protease is about the same size as GFP (~27 kDa), one TEV protease can cleave many GFP-REACh constructs per time than fluorescent protein molecules could be expressed and go through fluorescence maturation. Through this additional reaction, we introduce an '''amplification step''' into our system. Hypothetically, the TEV protease will enable the production of '''a much stronger signal''' in a given time interval.<br />
<br />
In parallel, we worked on a [https://2014.igem.org/Team:Aachen/Project/Model model] of the respective biosensor system, in order to confirm our hypothesis of a faster and stronger fluorescence response than a conventional biosensor would povide.<br />
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[[File:Aachen_14-10-13_FRET_Arrows_iNB.png|150px|right]]<br />
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=The FRET (Förster Resonance Energy Transfer) System=<br />
<span class="anchor" id="fret"></span><br />
<br />
Förster resonance energy transfer (FRET), sometimes also called fluorescence resonance energy transfer, is a physical process of energy transfer. In FRET, the energy of a donor chromophore, whose electrons are in an excited state, is passed to a second chromophore, the acceptor. The '''energy is transferred without radiation''' and is therefore not exchanged via emission and absorption of photons. The acceptor then releases the energy received from the donor, for example, as light of a longer wavelength. <br />
<br />
In biochemistry and cell biology fluorescent dyes, which interact via FRET, are applied as "optical nano metering rules", because the intensity of the transfer is dependent on the spacing between donor and acceptor. FRET can be observed over distances of up to 10&nbsp;nm. This way, protein-protein interactions and conformational changes of a variety of tagged biomolecules can be observed. For an efficient FRET to occur, there must be a substantial overlap between the donor fluorescence emission spectrum and the acceptor fluorescence excitation (or absorption) spectrum ([http://www.nature.com/nprot/journal/v8/n2/full/nprot.2012.147.html Broussard et al., 2013]), as described in the figure below.<br />
<br />
{{Team:Aachen/Figure|Aachen_14-10-07_Jablonski_Diagram_and_Absorption_Spectra_iNB.png|align=center|title=FRET betweeen donor and acceptor|subtitle=On the left: Jablonski diagram showing the transfer of energy between donor and acceptor; on the right: For successful FRET, the emission spectrum of of the donor has to overlap with the absorption spectrum of the acceptor.|width=900px}}<br />
<br />
However, '''fluorescence is not an essential requirement for FRET'''. This type of energy transfer can also be observed between donors that are capable of other forms of radiation, such as phosphorescence, bioluminescence or chemiluminescence. Acceptor chromophores do not necessarily emit the energy in form of light, and can lead to quenching instead. Thus, this kind of acceptors are also referred to as '''dark quenchers'''. In our project, we use a FRET system with a dark quencher, namely our '''REACh construct'''.<br />
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[[File:Aachen_14-10-13_REACh_iNB.png|150px|right]]<br />
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=REACh Proteins - Dark Quenchers of GFP=<br />
<span class="anchor" id="darkquencher"></span><br />
<br />
<center><br />
{{Team:Aachen/FigureFloat|Aachen_K1319000.gif|align=center|title=Homology model of REACh.|subtitle=This homology model was created with SWISS-MODEL. We used Chimera to prepare and export a scene that was then rendered into an animation with POV-Ray.|width=256px}}<br />
</center><br />
<br />
In 2006, [http://www.pnas.org/content/103/11/4089.full Ganesan et al.] were the first to present a previously undescribed FRET acceptor, a non-fluorescent yellow fluorescent protein (YFP) mutant called '''REACh (Resonance Energy-Accepting Chromoprotein)'''. YFP can be used as a FRET acceptor in combination with GFP as the donor in FRET microscopy and miscellaneous assays in molecular biology. The ideal FRET couple should possess a large spectral overlap between donor emission and acceptor absorption - as illustrated in previous section - but have separated emission spectra to allow their selective imaging.<br />
<br />
To optimize the spectral overlap of this FRET pair, the group obtained '''a genetically modified YFP acceptor'''. Mutations of amino acid residues that stabilize the excited state of the chromophore in enhanced YFP (EYFP) resulted in a non-fluorescent chromoprotein. Two mutations, H148V and Y145W, reduced the fluorescence emission by 82 and 98%, respectively. Ganesan et al. chose the Y145W mutant and the Y145W/H148V double mutant as FRET acceptors, and named them REACh1 and REACh2, respectively. '''Both REACh1 and REACh2 act as dark quenchers of GFP'''.<br />
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[[File:Aachen_14-10-13_Fusion_Protein_iNB.png|150px|right]]<br />
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=Producing a GFP-REACh Fusion Protein=<br />
<span class="anchor" id="gfp-reach"></span><br />
<br />
In our project, we reproduced the REACh1 and REACh2 proteins by subjecting an RFC-25 compatible version of the BioBrick [http://parts.igem.org/Part:BBa_E0030 E0030] (EYFP) to a '''QuikChange mutation''', creating the BioBricks [http://parts.igem.org/Part:BBa_K1319001 K1319001] and [http://parts.igem.org/Part:BBa_K1319002 K1319002]. Subsequently, we fused each REACh protein with '''GFP (mut3b)''' which is available as BioBrick [http://parts.igem.org/Part:BBa_E0040 E0040]. The protein complex was linked via a '''protease cleavage site''' ([http://parts.igem.org/Part:BBa_K1319016 K1319016]). As constitutive promoter we use [http://parts.igem.org/Part:BBa_J23101 J232101]. When GFP is connected to either REACh quencher, GFP will absorb light but the energy will be transferred to REACh via FRET and is then emitted in the form of heat; the fluorescence is quenched. Our cells also constitutively express the '''[http://parts.igem.org/Part:BBa_C0179 LasR] activator'''. Together with the HSL molecules from ''P. aeruginosa'', LasR binds to the '''[http://parts.igem.org/Part:BBa_J64010 LasI] promoter''' that controls the expression of the TEV protease, which we made available as [http://parts.igem.org/Part:BBa_K1319004 K1319004]. When the fusion protein is cleaved by the TEV protease, REACh will be separated from GFP. The latter will then be able to absorb and emit light as usual.<br />
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{{Team:Aachen/Figure|align=center|Aachen_14-10-08_REACh_approach_with_BioBricks_iNB.png|title= Composition of our biosensor|subtitle=For our biosensor, we use a mix of already available and self-constructed BioBricks.|width=900px}}<br />
<br />
The resulting fusion proteins can be expressed in [http://parts.igem.org/Part:BBa_K1319013 K1319013] (GFP fused with REACh1) and [http://parts.igem.org/Part:BBa_K1319014 K1319014] (GFP fused with REACh2). The linker between the proteins containing a TEV protease cleavage site is labelled as [http://parts.igem.org/Part:BBa_K1319016 K1319016].<br />
<br />
Since we did not have enough time to build the complete system we tested our FRET reporter with an IPTG inducible TEV protease ([http://parts.igem.org/Part:BBa_K1319008 K1319008]). In this way we were able to establish a proof of concept of our reporter system including proper expression of the TEV protease as well as functionality of our GFP-REACh construct.<br />
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{{Team:Aachen/Figure|align=center|Aachen 14-10-17 IPTG REACh iFG.png|title= Composition of our biosensor for IPTG|subtitle=To test the funtionality of our sensor concept, we expressed the TEV protease with a T7 promoter.|width=900px}}<br />
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{{Team:Aachen/BlockSeparator}}<br />
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[[File:Aachen_14-10-13_TEV_Protease_iNB.png|150px|right]]<br />
<br />
=Cutting the Fusion Protein with the TEV Protease=<br />
<span class="anchor" id="tevprotease"></span><br />
<br />
To cleave the GFP-REACh fusion protein, we chose '''Tobacco Etch Virus (TEV) protease''', a highly sequence-specific cysteine protease, that is frequently used for the controlled cleavage of fusion proteins ''in vitro'' and ''in vivo''. The native protease also contains an internal self-cleavage site. This site is slowly cleaved to inactivate the enzyme. The physiological reason for the self-cleavage is unknown, however, undesired for our use. Therefore, our team uses a variant of the native TEV protease containing the mutation S219V which results in an alteration of the cleavage site so that self-inactivation is diminished.<br />
<br />
{{Team:Aachen/Figure|Aachen_TEV_Protease_Model.png|title=TEV protease with a bound peptide|subtitle=This picture shows the TEV protease with a peptide chain bound in the binding pocket ready to be cleaved. The recognition site of the bound peptide chain is located inside the binding pocket of the TEV protease. It was rendered with POV-Ray.|width=800px}}<br />
<br />
Though quite popular in molecular biology, the TEV protease is not avaiable as a BioBrick yet. Hence, the team Aachen introduces the protease with anti-self cleavage mutation S219V and codon optimized for ''E. coli'' [http://parts.igem.org/Part:BBa_K1319004 '''to the Parts Registry this year.''']<br />
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{{Team:Aachen/BlockSeparator}}<br />
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[[File:Aachen_14-10-15_Medal_Cellocks_iNB.png|right|150px]]<br />
<br />
== Achievements ==<br />
<span class="anchor" id="reachachievements"></span> <br />
<br />
===Characterization of GFP-REACh1 and GFP-REACh2 fusion proteins in combination with an IPTG-inducible TEV Protease===<br />
<br />
The characterization of the TEV protease and the REACh1 and REACh2 dark quenchers was performed by introducing both simultaneously into ''E.&nbsp;coli'' Bl21 (DE3). The resulting double plasmid cells therefore contained [http://parts.igem.org/Part:BBa_K1319013 K1319013] (GFP-REACh1 fusion protein) or [http://parts.igem.org/Part:BBa_K1319014 K1319014] (GFP-REACh2 fusion protein) and [http://parts.igem.org/Part:BBa_K1319008 K1319008] (IPTG-inducible TEV protease). K1319013 and K1319014 were located on a pSB3K3 plasmid backbone and K1319008 on a pSB1C3 backbone, two standard iGEM plasmids with different oris allowing simultaneous use in one cell. <br />
<br />
[http://parts.igem.org/Part:BBa_I20260 I20260] was used as a positive control because I20260 contains the same promoter ([http://parts.igem.org/Part:BBa_J23101 J23101]), the same RBS ([http://parts.igem.org/Part:BBa_B0032 B0032]) and the same version of GFP ([http://parts.igem.org/Part:BBa_E0040 E0040]) and is located on the same plasmid backbone pSB3K3. Therefore, it is expected that when all fusion proteins are successfully cut by the TEV protease, the fluorescence level of the double plasmid constructs reaches the same level as the positive control of I20260. As a negative control [http://parts.igem.org/Part:BBa_B0015 B0015] was used, a coding sequence of a terminator which should not show any sign of fluorescence.<br />
<br />
To characterize our REACh1/2 constructs in combination with the TEV protease a growth experiment was conducted. Both of the double plasmid constructs, a constitutive expression of GFP (I20260) as positive control and B0015 as negative control were compared. For each expression IPTG-induced and non-induced cultures were grown in parallel. All measurements were done in a biological triplicate. <br />
<br />
To better evaluate the fluorescence, the observed Optical desity (OD) was taken into account in order to achieve a fluorescence measurement independent of the amount of cells present. This way, the measurement represents the amount of fluorescence per cell only. <br />
<br />
{{Team:Aachen/Figure|Aachen 16-10-14 Graph2iFG.PNG|title=Comparison of K1319013 + K1319008, K1319014 + K1319008, I20260 (positive control) and B0015 (negative control)|subtitle=Both double plasmid construct exhibit a clear fluorescence signal when induced.|width=700px}}<br />
<br />
The negative control B0015 did not exhibit any significant fluorescence. The positive control I20260 showed a steady level of fluorescence as expected due to the constitutive expression of GFP. As expected, the production is also independent of addition of IPTG therefore the triplicates have been merged together.<br />
<br />
Both double plasmid constructs K1319013 + K1319008 and K1319014 + K1319008 did not exhibit a strong fluorescence before induction with IPTG. In the non-induced state, the fluorescence stays low and does not increase over time. It is significantly weaker than the fluorescence reached by the induced constructs or the positive control but was higher than the negative control. The higher base level of fluorescence in the not induced constructs is due to the imperfect quenching and the leakiness of the IPTG inducible promoter. <br />
<br />
The induced double plasmid constructs exhibited a fast rise in fluorescence after induction. '''The signal strenght increased ~ 10-fold over the non-induced constructs.''' K1319014 + K1319008 reached the the same level of fluorescence as I20260, indicating a complete cleavage of the fusion proteins by the TEV protease. K1319013 + K1319008 did not reach a level of fluorescence as high as K1319013 + K1319008, however, the nearly 10-fold increase in fluorescence after induction is a clear indicator for the TEV protease cutting the fusion protein K1319013. The weaker fluorescence signal is probably due to a lower expression level of K1319013 in the cells compared to the expression level of K1319014. <br />
<br />
The fluorescence results of K1319014 + K1319008 was used to fit our [https://2014.igem.org/Team:Aachen/Project/Model model] to experimental data.<br />
<br />
====Summary====<br />
<br />
The double plasmid systems of K1319013 + K1319008 and K1319014 + K1319008 demonstrate the quenching ability of the REACh1 and REACh2 proteins as well as the funcionality of the TEV protease.<br />
The increase in fluorescence after induction with IPTG is based on a functional expression of the TEV protease which proceeds to cut the linker of the fusion protein already produced destroying the FRET system between GFP and its quencher and resulting in a strong fluorescence signal. Combined, this characterization is a '''validation of the functionality of the REACh1 protein ([http://parts.igem.org/Part:BBa_K1319001 K1319001]), the REACh2 protein ([http://parts.igem.org/Part:BBa_K1319002 K1319002]) and the TEV protease ([http://parts.igem.org/Part:BBa_K1319004 K1319004])'''.<br />
<br />
===Determining the Quenching ability of REACh1 and REACh2===<br />
<br />
In order to further evaluate the quenching ability of the REACh1 and REACh2 constructs in the fusion proteins produced by K1319013 and K1319014, they were expressed alone without an IPTG inducible TEV protease. This eliminated the effect of a potential leakiness of the non induced promoter to reliably assess the quenching ability of the REACh1 and REACh2 proteins. <br />
<br />
{{Team:Aachen/Figure|Aachen_K1319001_and_K1319002.PNG|title=Comparison of K1319013 and K1319014 with I20260 and B0015|subtitle=K1319013 and K1319014 show a severely reduced fluorescence compared to the positive control I20260.|width=700px}}<br />
<br />
In the previous experiment it was established that the fusion proteins K1319013 and K1319014 are expressed funtionally. K1319014 reached the same level of fluorescence as the positive control after being cut by the TEV protease. Therefore the reduced fluorescence in this experiment is completely attributable to the quenching of REACh1. The quenching reduces the fluorescence of GFP by a factor ~ 25 which means a '''quenching efficiency of ~ 96%!'''. <br />
<br />
K139013 was not able to reach the same fluorescence level as the positive control in the previous experiment. Therefore a worse rate of functional expression of K1319013 compared to K1319014 is assumed. incorporating this, the difference in fluorescence between induced and not induced is still a factor of ~ 30 resulting in a '''quenching efficiency of ~ 97%'''.<br />
<br />
====Summary ====<br />
<br />
The fusion proteins of GFP combined with REACh1 and REACh2 are not only fully functional but exhibit a great quenching efficiency of ~ 97% for REACh1 and ~ 96% for REACh2.Even though REACh1s quenching ability seems to be slighty superior, the expression level of the K1319014 fusion protein including REACh2 is nearly twice as high as the fusion protein K1319013 containing REACh1 and therefore shows a stronger fluorescence. Ganesan et al. (2006) reported a reduction on emission of GFP of 82% for REACh1 and 98% for REACh2 but with a different Linker between the proteins and on a different vector backbone.<br />
<br />
===Comparing fluorescence kinetics of the GFP-REACh fusion proteins with a Standard lacI-inducible GFP Expression===<br />
<br />
To assess the kinetics of the fusion proteins K1319013 (GFP-REACh1) and K1319014 (GFP-REACh2), the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 were compared to a standard expression of GFP under the control of a lacI promoter in [http://parts.igem.org/Part:BBa_K731520 K731520], a BioBrick made by the iGEM Team TRENTO in 2012. This tested the hypothesis of achieving a faster fluorescence response with the GFP-REACh fusion proteins compared to a standard expression.<br />
<br />
K731520 and the double plasmid constructs K1319013 + K1319008 and K1319014 + K1319008 were cultivated in ''E. coli'' BL21(DE3), and fluorescence and OD was measured.The fluorescence was adjusted for the OD to show a relative fluorescence on a per cell basis. The difference between the induced and non-induced state, the fluorescence quotient, serves as a better indicator for a system used as a sensor because the difference between an ''on'' and ''off'' state is more important for a clear and unmistakable signal than the overall fluorescence. Hence, the OD-adjusted fluorescence quotient for both double plasmid constructs and K731520 was obtained and plotted in the following graph.<br />
<br />
{{Team:Aachen/Figure|Aachen_16-10-14_GraphQuotient_iFG.PNG|Comparison of K1319013 + K1319008, K1319014 + K1319008 and K731520|subtitle=Fluorescence was normalized to the cell growth. The fluorescence of induced cells was additionally divided by the fluorescence of non-induced cells to obtain the fluorescence quotient.|width=700px}}<br />
<br />
The graph clearly shows the faster response of the cut GFP-REACh fusion protein compared to a standard GFP expression. Both fluorescence signals of the double plasmid constructs achieve a higher difference in fluorescence signal between induced and non-induced state as well as at a faster rate. This proves the hypothesis made earlier about the kinetics of the GFP-REACh fusion protein combined with the TEV protease.<br />
<br />
====Summary====<br />
<br />
The kinetics of the fusion protein combined with the TEV protease exhibits the exact characteristics as predicted. The response is clearly faster than normal expression by accumulating a reservoir of fusion proteins which are not fluorescing due to the dark quencher attached to them. This reservoir is then activated by the induction of the TEV protease expression. Production of the protease results in the cleavage of the fusion protein, releasing GFP from the dark quencher and disturbing the interaction between the FRET pair. This results in the observed faster fluorescence reaction due to the amplificating effect of the TEV protease in which every one TEV protease can account for many fluorescence proteins being activated.<br />
<br />
===Characterizing the GFP-REACh Constructs in Sensor Chips===<br />
To further characterize the REACh construct, they were introduced into the sensor cells which were then induced with 2&nbsp;µL IPTG with a concentration of 100&nbsp;mM. Subsequently, we took fluorescence measurement read-outs (GFP, excitation 496&nbsp;±&nbsp;9&nbsp;nm, emission&nbsp;516&nbsp;±&nbsp;9&nbsp;nm) roughly every 10&nbsp;min in the plate reader. The results were plotted in the heatmap shown here. <br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 40px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/6/6d/Aachen_K1319014%2B8_and_K1319013%2B8_pixelig_minus_bg.gif" width="500px"></html><br />
|-<br />
|'''{{{title|K1319014 + K1319008 and K1319013 + K1319008 non-induced (top) and induced (bottom) in sensor cells }}}'''<br />{{{subtitle|The induced double plasmid systems K1319013 + K1319008 and K1319014&nbsp;+&nbsp;K1319008 exhibit a clear fluorescence response in our sensor cells which in response to induction with 2 µl IPTG. }}}<br />
|}<br />
</div><br />
</center><br />
<br />
The heatmap shows an increase of fluorescence from blue (no fluorescence) to red (high fluorescence). It is clearly visible that the induced chips are exhibiting a significantly higher fluorescence than the non-induced chips. This shows that the constructs also work as intended in the sensor chips: The TEV protease cuts the linker so that the fusion protein is separated into GFP and a dark quencher, disabling the quenching. GFP has a clear fluorescence emission after the fusion protein has been successfully cut into two pieces by the TEV protease.<br />
<br />
===Comparing the kinetic of the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 with standard GFP expression===<br />
<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 40px; border:{{{border|0px solid #aaa}}};width:{{{width|480px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/1/1a/Aachen_K13%2B8%2CK14%2B8%2CK731_slower_reduced.gif" width="480px"></html><br />
|-<br />
|'''{{{title|K1319013 + K1319008, K1319014 + K1319008 and K731520 in an non-induced (top) and induced (bottom) chip}}}'''<br />{{{subtitle|Comparing the factor of fluorescence increase of induced (bottom) and not induced (top) sensor chips to the first frame of the measurement. The tested constructs are K1319013 + K1319008, K1319014 + K1319008 and K731520. After five hours, the fluorescence in our dual-plasmid REACh construct contaning K1319014 had increased by a factor of about 8, while K731520 fluorescence increased by only a factor of 2-3.}}}<br />
|}<br />
</div><br />
</center><br />
<br />
The analysis of the different [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor sensor chips] with the three different construct K1319013 + K1319008, K1319014 + K1319008 and K731520 demonstrates the same fluorescence response kinetic as in the shake flask experiments. The double plasmid systems exhibits a faster and stronger fluorescence response compared to a standard GFP expression in K731520. The build up pool of fusion proteins allows for a faster, stronger fluorescence response when the induced TEV protease cleaves the fusion proteins and releases GFP from its dark quencher. <br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-16_Outlook_Cellocks_iNB.png|right|150px]]<br />
<br />
== Outlook ==<br />
<span class="anchor" id="reachoutlook"></span><br />
<br />
The system of the GFP-REACh fusion proteins with an inducible TEV protease has been established and shows the desired results of being faster than standard expression. The next step will be to devise an expression of the TEV protease inducible by HSL instead of IPTG and then to incorporate both the HSL inducible TEV protease and the fusion protein into one plasmid backbone. This would also allow us to choose a high copy plasmid for both inserts, instead of a high copy plasmid for the TEV protease and a low to mid copy plasmid for the fusion protein which should yield an overall higher fluorescence readout.<br />
<br />
Subsequently the combined construct will be characterizes the same way as the double plasmid system and other options regarding different fluorescence proteins with different quenchers will be considered to be able to have multiple fluorescence responses readable at the same time while still being faster than normal expression. <br />
<br />
Also finding and testing different promoters which can be used to express the TEV protease is planned to be able to detect not only ''Pseudomonas aeruginosa'' but also other pathogens. Also the technology can be expanded to other molecules enabling other research areas to profit from the faster fluorescence response.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
= References =<br />
* Sundar Ganesan, Simon M. Ameer-Beg, Tony T. C. Ng, Borivoj Vojnovic and Fred S. Wouters. "A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP" Proceedings of the National Academy of Science of the United States of America | March 14,2006 | vol. 103 | no. 11 | 4089 - 4094<br />
<br />
* Broussard, Joshua A, Benjamin Rappaz, Donna J Webb, and Claire M Brown. "Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt." Nature protocols 8.2 (2013): 265-281. doi:10.1038/nprot.2012.147. <br />
<br />
* Broussard, J. A., Rappaz, B., Webb, D. J., & Brown, C. M. (2013). Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt. Nature protocols, 8(2), 265-281. doi: 10.1073/pnas.0509922103 <br />
<br />
'''SWISS-MODEL'''<br />
* Arnold, K., Bordoli, L., Kopp, J., & Schwede, T. (2005). The SWISS-MODEL Workspace: A Web-based Environment For Protein Structure Homology Modelling. Bioinformatics, 22(2), 195-201.<br />
<br />
* Biasini, M., Bienert, S., Schwede, T., Waterhouse, A., Arnold, K., Studer, G., et al. (2014). Nucleic Acids Research. SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information. doi: 10.1093/nar/gku340.<br />
<br />
* Guex, N., Peitsch, M. C., & Schwede, T. (2009). Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: A historical perspective. Electrophoresis, 30(S1), S162-S173.<br />
<br />
* Kiefer, F., Arnold, K., Kunzli, M., Bordoli, L., & Schwede, T. (2009). The SWISS-MODEL Repository and associated resources. Nucleic Acids Research, 37(Database), D387-D392.<br />
<br />
'''UCSF Chimera'''<br />
* Pettersen, E. F., Goddard, T. D., Huang, C. C., Couch, G. S., Greenblatt, D. M., Meng, E. C., et al. (2004). UCSF Chimera?A visualization system for exploratory research and analysis. Journal of Computational Chemistry, 25(13), 1605-1612. PubMed PMID: 15264254.<br />
<br />
'''POV-Ray'''<br />
* Persistence of Vision Pty. Ltd. (2004) Persistence of Vision Raytracer (Version 3.7) [Computer software]. Retrieved from http://www.povray.org/download/<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Project/FRET_ReporterTeam:Aachen/Project/FRET Reporter2014-10-18T03:44:48Z<p>Mosthege: /* Comparing the kinetic of the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 with standard GFP expression */</p>
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<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/Header}}<br />
<br />
=The modified REACh Construct=<br />
<br />
On this page, we present our biosensor on a molecular level. Here you can explore the different parts of our genetic device:<br />
<br />
<html><br />
<center><br />
<ul class="menusmall-grid" style="width:inherit;"><br />
<!-- Overview --><br />
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<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fluorescence" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">A Faster Answer</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0b/Aachen_14-10-13_GFP_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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</a><br />
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<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fret" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">The FRET System</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/54/Aachen_14-10-13_FRET_Arrows_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#darkquencher" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">REACh Quenchers</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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</a><br />
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<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#gfp-reach" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">The Fusion Protein</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
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</li><br />
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</center><br />
</html><br />
<br />
<html><br />
<center><br />
<ul class="menusmall-grid" style="width:516px;"><br />
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<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#tevprotease" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">TEV Protease</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/dc/Aachen_14-10-13_TEV_Protease_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">Achieve-<br/>ments</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/e/ef/Aachen_14-10-15_Medal_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
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<li style="width: 156px;margin-left: 8px;margin-right: 8px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachoutlook" style="color:black"><br />
<div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:40%;">Outlook</div></div><br />
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{{Team:Aachen/BlockSeparator}}<br />
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[[File:Aachen_14-10-13_GFP_iNB.png|150px|right]]<br />
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= A Fluorescence Answer Faster than Expression =<br />
<span class="anchor" id="fluorescence"></span><br />
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Biosensor systems are usually based on a reporter gene under the control of a promoter directly induced by the biomolecule of interest. In the case of our 2D biosensor for ''Pseudomonas&nbsp;aeruginosa'', the expression of our fluorescing reporter protein is directly induced by the activity of the bacterium's quorum sensing molecules. However, transcription, translation, folding and post-translational modifications take their time. Since our goal is to detect the pathogen as fast as possible, we aimed to develop a system that provides a fluorescent answer faster than just expressing the fluorescent protein GFP.<br />
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<center><br />
{{Team:Aachen/Figure|Aachen_Traditional_biosensor.png|align=center|title=Schematic model of a traditional biosensor|subtitle=In this model, the expression of GFP is directly controlled by a promoter whose activator binds to a molecule secreted by the pathogen.|width=500px}}<br />
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As an alternative to the traditional approach, we '''constitutively express our reporter gene in a quenched form'''. By fusing GFP to REACh, a mutated variant of EYFP, its original fluorescence is thoroughly suppressed. Thereby, a huge stock of temporary inactivated fluorescence proteins is already available prior to first contact with the pathogen. As a conseqence to the uptake of homoserine lactones of ''P.&nbsp;aeruginosa'' and binding to the LasI promoter in front of the protease gene, these '''autoinducers activate the expression of the TEV protease'''. This way, our biosensor provides a fast increase in fluorescence intensity by cleavage of GFP-REACh-constructs.<br />
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<center><br />
{{Team:Aachen/Figure|Aachen_REACh_approach.png|align=center|title=Schematic model of our novel biosensor|subtitle=Expression of the TEV protease is induced by HSL. The protease cleaves the GFP-REACh fusion protein to elicit a fluorescence response.|width=900px}}<br />
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'''Advantages of the novel biosensor:'''<br />
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* When ''P.&nbsp;aeruginosa'' is detected by our cells, the reporter protein has already been expressed and only waits to be activated. The cleavage reaction catalyzed by the TEV protease is a faster process than expression and correct folding of GFP. Therefore, our sensor cells provide an exceptionally '''early response'''.<br />
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* While a certain concentration of homoserine lactone will produce the same number of gene read-outs and the TEV protease is about the same size as GFP (~27 kDa), one TEV protease can cleave many GFP-REACh constructs per time than fluorescent protein molecules could be expressed and go through fluorescence maturation. Through this additional reaction, we introduce an '''amplification step''' into our system. Hypothetically, the TEV protease will enable the production of '''a much stronger signal''' in a given time interval.<br />
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In parallel, we worked on a [https://2014.igem.org/Team:Aachen/Project/Model model] of the respective biosensor system, in order to confirm our hypothesis of a faster and stronger fluorescence response than a conventional biosensor would povide.<br />
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[[File:Aachen_14-10-13_FRET_Arrows_iNB.png|150px|right]]<br />
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=The FRET (Förster Resonance Energy Transfer) System=<br />
<span class="anchor" id="fret"></span><br />
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Förster resonance energy transfer (FRET), sometimes also called fluorescence resonance energy transfer, is a physical process of energy transfer. In FRET, the energy of a donor chromophore, whose electrons are in an excited state, is passed to a second chromophore, the acceptor. The '''energy is transferred without radiation''' and is therefore not exchanged via emission and absorption of photons. The acceptor then releases the energy received from the donor, for example, as light of a longer wavelength. <br />
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In biochemistry and cell biology fluorescent dyes, which interact via FRET, are applied as "optical nano metering rules", because the intensity of the transfer is dependent on the spacing between donor and acceptor. FRET can be observed over distances of up to 10&nbsp;nm. This way, protein-protein interactions and conformational changes of a variety of tagged biomolecules can be observed. For an efficient FRET to occur, there must be a substantial overlap between the donor fluorescence emission spectrum and the acceptor fluorescence excitation (or absorption) spectrum ([http://www.nature.com/nprot/journal/v8/n2/full/nprot.2012.147.html Broussard et al., 2013]), as described in the figure below.<br />
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{{Team:Aachen/Figure|Aachen_14-10-07_Jablonski_Diagram_and_Absorption_Spectra_iNB.png|align=center|title=FRET betweeen donor and acceptor|subtitle=On the left: Jablonski diagram showing the transfer of energy between donor and acceptor; on the right: For successful FRET, the emission spectrum of of the donor has to overlap with the absorption spectrum of the acceptor.|width=900px}}<br />
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However, '''fluorescence is not an essential requirement for FRET'''. This type of energy transfer can also be observed between donors that are capable of other forms of radiation, such as phosphorescence, bioluminescence or chemiluminescence. Acceptor chromophores do not necessarily emit the energy in form of light, and can lead to quenching instead. Thus, this kind of acceptors are also referred to as '''dark quenchers'''. In our project, we use a FRET system with a dark quencher, namely our '''REACh construct'''.<br />
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[[File:Aachen_14-10-13_REACh_iNB.png|150px|right]]<br />
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=REACh Proteins - Dark Quenchers of GFP=<br />
<span class="anchor" id="darkquencher"></span><br />
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{{Team:Aachen/FigureFloat|Aachen_K1319000.gif|align=center|title=Homology model of REACh.|subtitle=This homology model was created with SWISS-MODEL. We used Chimera to prepare and export a scene that was then rendered into an animation with POV-Ray.|width=256px}}<br />
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In 2006, [http://www.pnas.org/content/103/11/4089.full Ganesan et al.] were the first to present a previously undescribed FRET acceptor, a non-fluorescent yellow fluorescent protein (YFP) mutant called '''REACh (Resonance Energy-Accepting Chromoprotein)'''. YFP can be used as a FRET acceptor in combination with GFP as the donor in FRET microscopy and miscellaneous assays in molecular biology. The ideal FRET couple should possess a large spectral overlap between donor emission and acceptor absorption - as illustrated in previous section - but have separated emission spectra to allow their selective imaging.<br />
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To optimize the spectral overlap of this FRET pair, the group obtained '''a genetically modified YFP acceptor'''. Mutations of amino acid residues that stabilize the excited state of the chromophore in enhanced YFP (EYFP) resulted in a non-fluorescent chromoprotein. Two mutations, H148V and Y145W, reduced the fluorescence emission by 82 and 98%, respectively. Ganesan et al. chose the Y145W mutant and the Y145W/H148V double mutant as FRET acceptors, and named them REACh1 and REACh2, respectively. '''Both REACh1 and REACh2 act as dark quenchers of GFP'''.<br />
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[[File:Aachen_14-10-13_Fusion_Protein_iNB.png|150px|right]]<br />
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=Producing a GFP-REACh Fusion Protein=<br />
<span class="anchor" id="gfp-reach"></span><br />
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In our project, we reproduced the REACh1 and REACh2 proteins by subjecting an RFC-25 compatible version of the BioBrick [http://parts.igem.org/Part:BBa_E0030 E0030] (EYFP) to a '''QuikChange mutation''', creating the BioBricks [http://parts.igem.org/Part:BBa_K1319001 K1319001] and [http://parts.igem.org/Part:BBa_K1319002 K1319002]. Subsequently, we fused each REACh protein with '''GFP (mut3b)''' which is available as BioBrick [http://parts.igem.org/Part:BBa_E0040 E0040]. The protein complex was linked via a '''protease cleavage site''' ([http://parts.igem.org/Part:BBa_K1319016 K1319016]). As constitutive promoter we use [http://parts.igem.org/Part:BBa_J23101 J232101]. When GFP is connected to either REACh quencher, GFP will absorb light but the energy will be transferred to REACh via FRET and is then emitted in the form of heat; the fluorescence is quenched. Our cells also constitutively express the '''[http://parts.igem.org/Part:BBa_C0179 LasR] activator'''. Together with the HSL molecules from ''P. aeruginosa'', LasR binds to the '''[http://parts.igem.org/Part:BBa_J64010 LasI] promoter''' that controls the expression of the TEV protease, which we made available as [http://parts.igem.org/Part:BBa_K1319004 K1319004]. When the fusion protein is cleaved by the TEV protease, REACh will be separated from GFP. The latter will then be able to absorb and emit light as usual.<br />
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{{Team:Aachen/Figure|align=center|Aachen_14-10-08_REACh_approach_with_BioBricks_iNB.png|title= Composition of our biosensor|subtitle=For our biosensor, we use a mix of already available and self-constructed BioBricks.|width=900px}}<br />
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The resulting fusion proteins can be expressed in [http://parts.igem.org/Part:BBa_K1319013 K1319013] (GFP fused with REACh1) and [http://parts.igem.org/Part:BBa_K1319014 K1319014] (GFP fused with REACh2). The linker between the proteins containing a TEV protease cleavage site is labelled as [http://parts.igem.org/Part:BBa_K1319016 K1319016].<br />
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Since we did not have enough time to build the complete system we tested our FRET reporter with an IPTG inducible TEV protease ([http://parts.igem.org/Part:BBa_K1319008 K1319008]). In this way we were able to establish a proof of concept of our reporter system including proper expression of the TEV protease as well as functionality of our GFP-REACh construct.<br />
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{{Team:Aachen/Figure|align=center|Aachen 14-10-17 IPTG REACh iFG.png|title= Composition of our biosensor for IPTG|subtitle=To test the funtionality of our sensor concept, we expressed the TEV protease with a T7 promoter.|width=900px}}<br />
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[[File:Aachen_14-10-13_TEV_Protease_iNB.png|150px|right]]<br />
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=Cutting the Fusion Protein with the TEV Protease=<br />
<span class="anchor" id="tevprotease"></span><br />
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To cleave the GFP-REACh fusion protein, we chose '''Tobacco Etch Virus (TEV) protease''', a highly sequence-specific cysteine protease, that is frequently used for the controlled cleavage of fusion proteins ''in vitro'' and ''in vivo''. The native protease also contains an internal self-cleavage site. This site is slowly cleaved to inactivate the enzyme. The physiological reason for the self-cleavage is unknown, however, undesired for our use. Therefore, our team uses a variant of the native TEV protease containing the mutation S219V which results in an alteration of the cleavage site so that self-inactivation is diminished.<br />
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{{Team:Aachen/Figure|Aachen_TEV_Protease_Model.png|title=TEV protease with a bound peptide|subtitle=This picture shows the TEV protease with a peptide chain bound in the binding pocket ready to be cleaved. The recognition site of the bound peptide chain is located inside the binding pocket of the TEV protease. It was rendered with POV-Ray.|width=800px}}<br />
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Though quite popular in molecular biology, the TEV protease is not avaiable as a BioBrick yet. Hence, the team Aachen introduces the protease with anti-self cleavage mutation S219V and codon optimized for ''E. coli'' [http://parts.igem.org/Part:BBa_K1319004 '''to the Parts Registry this year.''']<br />
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[[File:Aachen_14-10-15_Medal_Cellocks_iNB.png|right|150px]]<br />
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== Achievements ==<br />
<span class="anchor" id="reachachievements"></span> <br />
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===Characterization of GFP-REACh1 and GFP-REACh2 fusion proteins in combination with an IPTG-inducible TEV Protease===<br />
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The characterization of the TEV protease and the REACh1 and REACh2 dark quenchers was performed by introducing both simultaneously into ''E.&nbsp;coli'' Bl21 (DE3). The resulting double plasmid cells therefore contained [http://parts.igem.org/Part:BBa_K1319013 K1319013] (GFP-REACh1 fusion protein) or [http://parts.igem.org/Part:BBa_K1319014 K1319014] (GFP-REACh2 fusion protein) and [http://parts.igem.org/Part:BBa_K1319008 K1319008] (IPTG-inducible TEV protease). K1319013 and K1319014 were located on a pSB3K3 plasmid backbone and K1319008 on a pSB1C3 backbone, two standard iGEM plasmids with different oris allowing simultaneous use in one cell. <br />
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[http://parts.igem.org/Part:BBa_I20260 I20260] was used as a positive control because I20260 contains the same promoter ([http://parts.igem.org/Part:BBa_J23101 J23101]), the same RBS ([http://parts.igem.org/Part:BBa_B0032 B0032]) and the same version of GFP ([http://parts.igem.org/Part:BBa_E0040 E0040]) and is located on the same plasmid backbone pSB3K3. Therefore, it is expected that when all fusion proteins are successfully cut by the TEV protease, the fluorescence level of the double plasmid constructs reaches the same level as the positive control of I20260. As a negative control [http://parts.igem.org/Part:BBa_B0015 B0015] was used, a coding sequence of a terminator which should not show any sign of fluorescence.<br />
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To characterize our REACh1/2 constructs in combination with the TEV protease a growth experiment was conducted. Both of the double plasmid constructs, a constitutive expression of GFP (I20260) as positive control and B0015 as negative control were compared. For each expression IPTG-induced and non-induced cultures were grown in parallel. All measurements were done in a biological triplicate. <br />
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To better evaluate the fluorescence, the observed Optical desity (OD) was taken into account in order to achieve a fluorescence measurement independent of the amount of cells present. This way, the measurement represents the amount of fluorescence per cell only. <br />
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{{Team:Aachen/Figure|Aachen 16-10-14 Graph2iFG.PNG|title=Comparison of K1319013 + K1319008, K1319014 + K1319008, I20260 (positive control) and B0015 (negative control)|subtitle=Both double plasmid construct exhibit a clear fluorescence signal when induced.|width=700px}}<br />
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The negative control B0015 did not exhibit any significant fluorescence. The positive control I20260 showed a steady level of fluorescence as expected due to the constitutive expression of GFP. As expected, the production is also independent of addition of IPTG therefore the triplicates have been merged together.<br />
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Both double plasmid constructs K1319013 + K1319008 and K1319014 + K1319008 did not exhibit a strong fluorescence before induction with IPTG. In the non-induced state, the fluorescence stays low and does not increase over time. It is significantly weaker than the fluorescence reached by the induced constructs or the positive control but was higher than the negative control. The higher base level of fluorescence in the not induced constructs is due to the imperfect quenching and the leakiness of the IPTG inducible promoter. <br />
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The induced double plasmid constructs exhibited a fast rise in fluorescence after induction. '''The signal strenght increased ~ 10-fold over the non-induced constructs.''' K1319014 + K1319008 reached the the same level of fluorescence as I20260, indicating a complete cleavage of the fusion proteins by the TEV protease. K1319013 + K1319008 did not reach a level of fluorescence as high as K1319013 + K1319008, however, the nearly 10-fold increase in fluorescence after induction is a clear indicator for the TEV protease cutting the fusion protein K1319013. The weaker fluorescence signal is probably due to a lower expression level of K1319013 in the cells compared to the expression level of K1319014. <br />
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The fluorescence results of K1319014 + K1319008 was used to fit our [https://2014.igem.org/Team:Aachen/Project/Model model] to experimental data.<br />
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====Summary====<br />
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The double plasmid systems of K1319013 + K1319008 and K1319014 + K1319008 demonstrate the quenching ability of the REACh1 and REACh2 proteins as well as the funcionality of the TEV protease.<br />
The increase in fluorescence after induction with IPTG is based on a functional expression of the TEV protease which proceeds to cut the linker of the fusion protein already produced destroying the FRET system between GFP and its quencher and resulting in a strong fluorescence signal. Combined, this characterization is a '''validation of the functionality of the REACh1 protein ([http://parts.igem.org/Part:BBa_K1319001 K1319001]), the REACh2 protein ([http://parts.igem.org/Part:BBa_K1319002 K1319002]) and the TEV protease ([http://parts.igem.org/Part:BBa_K1319004 K1319004])'''.<br />
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===Determining the Quenching ability of REACh1 and REACh2===<br />
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In order to further evaluate the quenching ability of the REACh1 and REACh2 constructs in the fusion proteins produced by K1319013 and K1319014, they were expressed alone without an IPTG inducible TEV protease. This eliminated the effect of a potential leakiness of the non induced promoter to reliably assess the quenching ability of the REACh1 and REACh2 proteins. <br />
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{{Team:Aachen/Figure|Aachen_K1319001_and_K1319002.PNG|title=Comparison of K1319013 and K1319014 with I20260 and B0015|subtitle=K1319013 and K1319014 show a severely reduced fluorescence compared to the positive control I20260.|width=700px}}<br />
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In the previous experiment it was established that the fusion proteins K1319013 and K1319014 are expressed funtionally. K1319014 reached the same level of fluorescence as the positive control after being cut by the TEV protease. Therefore the reduced fluorescence in this experiment is completely attributable to the quenching of REACh1. The quenching reduces the fluorescence of GFP by a factor ~ 25 which means a '''quenching efficiency of ~ 96%!'''. <br />
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K139013 was not able to reach the same fluorescence level as the positive control in the previous experiment. Therefore a worse rate of functional expression of K1319013 compared to K1319014 is assumed. incorporating this, the difference in fluorescence between induced and not induced is still a factor of ~ 30 resulting in a '''quenching efficiency of ~ 97%'''.<br />
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====Summary ====<br />
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The fusion proteins of GFP combined with REACh1 and REACh2 are not only fully functional but exhibit a great quenching efficiency of ~ 97% for REACh1 and ~ 96% for REACh2.Even though REACh1s quenching ability seems to be slighty superior, the expression level of the K1319014 fusion protein including REACh2 is nearly twice as high as the fusion protein K1319013 containing REACh1 and therefore shows a stronger fluorescence. Ganesan et al. (2006) reported a reduction on emission of GFP of 82% for REACh1 and 98% for REACh2 but with a different Linker between the proteins and on a different vector backbone.<br />
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===Comparing fluorescence kinetics of the GFP-REACh fusion proteins with a Standard lacI-inducible GFP Expression===<br />
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To assess the kinetics of the fusion proteins K1319013 (GFP-REACh1) and K1319014 (GFP-REACh2), the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 were compared to a standard expression of GFP under the control of a lacI promoter in [http://parts.igem.org/Part:BBa_K731520 K731520], a BioBrick made by the iGEM Team TRENTO in 2012. This tested the hypothesis of achieving a faster fluorescence response with the GFP-REACh fusion proteins compared to a standard expression.<br />
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K731520 and the double plasmid constructs K1319013 + K1319008 and K1319014 + K1319008 were cultivated in ''E. coli'' BL21(DE3), and fluorescence and OD was measured.The fluorescence was adjusted for the OD to show a relative fluorescence on a per cell basis. The difference between the induced and non-induced state, the fluorescence quotient, serves as a better indicator for a system used as a sensor because the difference between an ''on'' and ''off'' state is more important for a clear and unmistakable signal than the overall fluorescence. Hence, the OD-adjusted fluorescence quotient for both double plasmid constructs and K731520 was obtained and plotted in the following graph.<br />
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{{Team:Aachen/Figure|Aachen_16-10-14_GraphQuotient_iFG.PNG|Comparison of K1319013 + K1319008, K1319014 + K1319008 and K731520|subtitle=Fluorescence was normalized to the cell growth. The fluorescence of induced cells was additionally divided by the fluorescence of non-induced cells to obtain the fluorescence quotient.|width=700px}}<br />
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The graph clearly shows the faster response of the cut GFP-REACh fusion protein compared to a standard GFP expression. Both fluorescence signals of the double plasmid constructs achieve a higher difference in fluorescence signal between induced and non-induced state as well as at a faster rate. This proves the hypothesis made earlier about the kinetics of the GFP-REACh fusion protein combined with the TEV protease.<br />
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====Summary====<br />
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The kinetics of the fusion protein combined with the TEV protease exhibits the exact characteristics as predicted. The response is clearly faster than normal expression by accumulating a reservoir of fusion proteins which are not fluorescing due to the dark quencher attached to them. This reservoir is then activated by the induction of the TEV protease expression. Production of the protease results in the cleavage of the fusion protein, releasing GFP from the dark quencher and disturbing the interaction between the FRET pair. This results in the observed faster fluorescence reaction due to the amplificating effect of the TEV protease in which every one TEV protease can account for many fluorescence proteins being activated.<br />
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===Characterizing the GFP-REACh Constructs in Sensor Chips===<br />
To further characterize the REACh construct, they were introduced into the sensor cells which were then induced with 2&nbsp;µL IPTG with a concentration of 100&nbsp;mM. Subsequently, we took fluorescence measurement read-outs (GFP, excitation 496&nbsp;±&nbsp;9&nbsp;nm, emission&nbsp;516&nbsp;±&nbsp;9&nbsp;nm) roughly every 10&nbsp;min in the plate reader. The results were plotted in the heatmap shown here. <br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 40px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/6/6d/Aachen_K1319014%2B8_and_K1319013%2B8_pixelig_minus_bg.gif" width="500px"></html><br />
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|'''{{{title|K1319014 + K1319008 and K1319013 + K1319008 non-induced (top) and induced (bottom) in sensor cells }}}'''<br />{{{subtitle|The induced double plasmid systems K1319013 + K1319008 and K1319014&nbsp;+&nbsp;K1319008 exhibit a clear fluorescence response in our sensor cells which in response to induction with 2 µl IPTG. }}}<br />
|}<br />
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</center><br />
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The heatmap shows an increase of fluorescence from blue (no fluorescence) to red (high fluorescence). It is clearly visible that the induced chips are exhibiting a significantly higher fluorescence than the non-induced chips. This shows that the constructs also work as intended in the sensor chips: The TEV protease cuts the linker so that the fusion protein is separated into GFP and a dark quencher, disabling the quenching. GFP has a clear fluorescence emission after the fusion protein has been successfully cut into two pieces by the TEV protease.<br />
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===Comparing the kinetic of the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 with standard GFP expression===<br />
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<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 40px; border:{{{border|0px solid #aaa}}};width:{{{width|480px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/1/1a/Aachen_K13%2B8%2CK14%2B8%2CK731_slower_reduced.gif" width="480px"></html><br />
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|'''{{{title|K1319013 + K1319008, K1319014 + K1319008 and K731520 in an non-induced (top) and induced (bottom) chip}}}'''<br />{{{subtitle|Comparing the factor of fluorescence increase of induced (bottom) and not induced (top) sensor chips to the first frame of the measurement. The tested constructs are K1319013 + K1319008, K1319014 + K1319008 and K731520.}}}<br />
|}<br />
</div><br />
</center><br />
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The analysis of the different [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor sensor chips] with the three different construct K1319013 + K1319008, K1319014 + K1319008 and K731520 demonstrates the same fluorescence response kinetic as in the shake flask experiments. The double plasmid systems exhibits a faster and stronger fluorescence response compared to a standard GFP expression in K731520. The build up pool of fusion proteins allows for a faster, stronger fluorescence response when the induced TEV protease cleaves the fusion proteins and releases GFP from its dark quencher. <br />
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== Outlook ==<br />
<span class="anchor" id="reachoutlook"></span><br />
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The system of the GFP-REACh fusion proteins with an inducible TEV protease has been established and shows the desired results of being faster than standard expression. The next step will be to devise an expression of the TEV protease inducible by HSL instead of IPTG and then to incorporate both the HSL inducible TEV protease and the fusion protein into one plasmid backbone. This would also allow us to choose a high copy plasmid for both inserts, instead of a high copy plasmid for the TEV protease and a low to mid copy plasmid for the fusion protein which should yield an overall higher fluorescence readout.<br />
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Subsequently the combined construct will be characterizes the same way as the double plasmid system and other options regarding different fluorescence proteins with different quenchers will be considered to be able to have multiple fluorescence responses readable at the same time while still being faster than normal expression. <br />
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Also finding and testing different promoters which can be used to express the TEV protease is planned to be able to detect not only ''Pseudomonas aeruginosa'' but also other pathogens. Also the technology can be expanded to other molecules enabling other research areas to profit from the faster fluorescence response.<br />
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= References =<br />
* Sundar Ganesan, Simon M. Ameer-Beg, Tony T. C. Ng, Borivoj Vojnovic and Fred S. Wouters. "A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP" Proceedings of the National Academy of Science of the United States of America | March 14,2006 | vol. 103 | no. 11 | 4089 - 4094<br />
<br />
* Broussard, Joshua A, Benjamin Rappaz, Donna J Webb, and Claire M Brown. "Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt." Nature protocols 8.2 (2013): 265-281. doi:10.1038/nprot.2012.147. <br />
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* Broussard, J. A., Rappaz, B., Webb, D. J., & Brown, C. M. (2013). Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt. Nature protocols, 8(2), 265-281. doi: 10.1073/pnas.0509922103 <br />
<br />
'''SWISS-MODEL'''<br />
* Arnold, K., Bordoli, L., Kopp, J., & Schwede, T. (2005). The SWISS-MODEL Workspace: A Web-based Environment For Protein Structure Homology Modelling. Bioinformatics, 22(2), 195-201.<br />
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* Biasini, M., Bienert, S., Schwede, T., Waterhouse, A., Arnold, K., Studer, G., et al. (2014). Nucleic Acids Research. SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information. doi: 10.1093/nar/gku340.<br />
<br />
* Guex, N., Peitsch, M. C., & Schwede, T. (2009). Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: A historical perspective. Electrophoresis, 30(S1), S162-S173.<br />
<br />
* Kiefer, F., Arnold, K., Kunzli, M., Bordoli, L., & Schwede, T. (2009). The SWISS-MODEL Repository and associated resources. Nucleic Acids Research, 37(Database), D387-D392.<br />
<br />
'''UCSF Chimera'''<br />
* Pettersen, E. F., Goddard, T. D., Huang, C. C., Couch, G. S., Greenblatt, D. M., Meng, E. C., et al. (2004). UCSF Chimera?A visualization system for exploratory research and analysis. Journal of Computational Chemistry, 25(13), 1605-1612. PubMed PMID: 15264254.<br />
<br />
'''POV-Ray'''<br />
* Persistence of Vision Pty. Ltd. (2004) Persistence of Vision Raytracer (Version 3.7) [Computer software]. Retrieved from http://www.povray.org/download/<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Project/2D_BiosensorTeam:Aachen/Project/2D Biosensor2014-10-18T03:41:25Z<p>Mosthege: /* Development & Optimization */</p>
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= 2D Biosensor =<br />
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With our 2D biosensor technology we are able to detect the pathogen ''Pseudomonas aeruginosa'' on solid surfaces. The sensor system is comprised of '''two distinct but inseparable modules''', a biological and a technical part:<br />
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* Sensor chips containing '''''Cellocks''''', our '''engineered detective cells''' that fluoresce in the presence of the pathogen, make up the biological part of ''Cellock Holmes''.<br />
* Our '''measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn'']''' and the complementary '''software [https://2014.igem.org/Team:Aachen/Notebook/Software/Measurarty ''Measurarty'']''' complete our sensing technology on the technical side. <br />
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== Principle of Operation ==<br />
<span class="anchor" id="biosensorpoo"></span><br />
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''Cellock Holmes'' is designed upon a SynBio approach comprising a '''two-dimensional biosensor and a measurement unit'''. The two-dimensional biosensor is devised to recognize quorum sensing molecules secreted by the pathogen cells and to generate a distinct fluorescence signal; while the measurement device recognizes and analyzes the produced signal. On the molecular side, we use the '''[https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh construct]''' to transform regular ''E. coli'' cells into ''Cellocks''.<br />
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{{Team:Aachen/Figure|Aachen 17-10-14 The basics of quorum sensing ipo.png||title=The principle of quorum sensing|subtitle=Microorganisms can sense the presence of their own kind based on quorum sensing which is a form of chemical communication. Depending on their cell density, quorum sensing allows these cells to activate or deactivate certain gene expression cascades (Waters and Bassler, 2005) for a specific function.|width=900px}}<br />
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Our '''sensor cells, ''Cellocks'', are immobilized in agar chips'''. To make the chips, we mix the ''Cellocks'' with liquid LB agar. <br />
In the course of our project, we designed a casting mold specifically for the production of our agar chips. When the agar has cooled down, the chips are cut out of the mold and are ready to use. Storage of the readily usable sensor chips is possible for up to two days at 4°C when using LB medium or up to five days if TB medium is used. A detailed description of the sensor chip manufacturing can be found in our [https://2014.igem.org/Team:Aachen/Notebook/Protocols/detection Protocols] section.<br />
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Assay to detect ''P.&nbsp;aeruginosa'' using ''Cellock Holmes''|subtitle=This flow sheet shows the procedure to sample and detect ''P.&nbsp;aeruginosa'': A sampling chip is briefly put onto the potentially contaminated surface, added onto one of our sensor chips and inserted into ''WatsOn''.|width=900px}}<br />
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Using ''Cellock Holmes'', we developed a simple assay to detect ''P.&nbsp;aeruginosa''. Initially, a so called sampling chip is placed on a solid surface that is potentially contaminated with the pathogen. Subsequently, the sampling chip is removed from the surface and put onto one of our sensor chips. Theorectically, the sensor chips could be directly used for sampling, however, this was avoided in our project to '''match [https://2014.igem.org/Team:Aachen/Safety biosafety regulations]''' and to prevent the spread of genetically modified organisms (GMOs) into the environment. The two layered chip-stack is then put into a petri dish which is inserted into our measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] for evalutation.<br />
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside ''WatsOn''|subtitle=Chips are incubated at 37°C to stimulate cell growth and then illuminated with blue light to excite fluorescence. A picture is taken and analyzed for fluorescence signals using the software ''Measurarty''.|width=900px}}<br />
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Inside ''WatsOn'', the chips are incubated at 37°C and the sampled populations of microorganisms attached on the sampling chip start to grow and multiply. During incubation the chips can be '''illuminated with blue light''' at any time, and a '''photo of the chips''' is taken. The '''software ''Measurarty''''' then analyzes any fluorescent signal. ''P.&nbsp;aeruginosa'' secrets an increasing number of quorum sensing molecules that are recognized by ''Cellocks'', thereby producing a fluorescence signal. For detection of ''P.&nbsp;aeruginosa'', we focused on a quorum sensing molecule called N-3-oxo-dodecanoyl-L-homoserine lactone (for short: 3-oxo-C<sub>12</sub>-HSL), which is involved in virulence regulation of ''P.&nbsp;aeruginosa'' (Jimenez, Koch, Thompson et al., 2012). The incorporation of the 3-oxo-C<sub>12</sub>-HSL detection system into the ''Cellocks'' is explained in detail in the [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh Construct] section.<br />
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For our biosensor, our team genetically modified ''E. coli'' cells to be able to elecit a '''fluorescent response to autoinducers''' produced by the pathogen ''Pseudomonas aeruginosa'' during quorum sensing. In the case of ''P.&nbsp;aeruginosa'', these autoinducers are N-3-oxo-dodecanoyl-L-homoserine lactone, or 3-oxo-C-12-HSL for short. The quorum sensing system of this pathogen contains the '''LasR activator''' which binds 3-oxo-C-12-HSL, and the '''LasI promoter''', which is activated by the LasR-HSL complex. Both LasR activator and LasI promoter are available as BioBricks [http://parts.igem.org/Part:BBa_C0179 C0179] and [http://parts.igem.org/Part:BBa_J64010 J64010].<br />
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As a reporter gene, we use '''GFP'''. However, expression of GFP is not simply controlled through the LasI promoter activity in our approach. Instead, our sensor cells contain genes for a constitutively expressed fusion protein consisting of GFP and a dark quencher, and an '''HSL-inducible protease'''. We use the REACh protein as dark quencher for GFP and the TEV protease to cleave the complex; [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter here] you can read more about the REACh construct and the TEV protease. <br />
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{{Team:Aachen/Figure|align=center|Aachen_REACh_approach.png|title=Our novel biosensor approach|subtitle=Expression of the TEV protease is induced by HSL. The protease cleaves the GFP-REACh fusion protein to elecit a fluorescence response.|width=500px}}<br />
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When ''P.&nbsp;aeruginosa cells'' are stuck on our agar chip and come close to our sensor cells, the latter take up the HSL molecules secreted by the pathogens. Inside the sensor cells, the autoinducer binds to the LasR gene product and activate the expression of the TEV protease. The protease then cleaves the GFP-REACh construct. When '''illuminated with light of 480&nbsp;nm''', the excitation wavelenght of GFP, our sensor cells in the vicinity of ''P.&nbsp;aeruginosa'' give a '''fluorescence signal'''. On the other hand, sensor cells that were not anywhere close to the pathogens do not express the protease. Therefore, the GFP will still be attached to the dark quencher in these cells, and no fluorescence is produced.<br />
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== Development & Optimization ==<br />
<span class="anchor" id="biosensordevelopment"></span><br />
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{{Team:Aachen/FigureFloat|Aachen_ILOV_GFP_HM_1,5h.png|title=iLOV and GFP in the Gel Doc<sup>TM</sup>|subtitle=Sensor cells producing iLOV (K1319042, A) and GFP (B) 1.5&nbsp;h after induction.|left|width=500px}}<br />
=== Equipment and medium selection ===<br />
Our first approach (before developing our own device) was to use the Molecular Imager&reg; Gel Doc™ XR+ from BIO-RAD in our lab to detect fluorescence. This device uses UV and white light illuminators. However, only two different filters were available for the excitation light wavelength, which resulted in very limited possibilities for the excitation of fluorescent molecules. For example, it was possible to detect the expression of iLOV (K1319042) in our sensor chips, but not the expression of GFP. Hence, the '''Gel Doc™ was not suitable for our project'''.<br />
{{Team:Aachen/FigureFloat|Aachen_Chip_medium_geldoc.png|title=Differend medium in the Gel Doc™|subtitle=complex media exhibited high background fluorescence while less back- ground fluorescence was observed with the minimal media (HM, M9, NA).|right|width=500px}}<br />
{{Team:Aachen/FigureFloat|Aachen_5days_K131026_neb_tb_1,5h.jpg |title=Testing our chips' shelf-life|subtitle= Chips of [http://parts.igem.org/Part:BBa_K131026 K131026] in NEB were stored five days at 4°C. The right chip was induced with 0.2&nbsp;µL of 500&nbsp;µg/mL HSL and an image was taken after 1.5&nbsp;h.|left|width=500px}}<br />
We tested different media (LB, TB, M9, NA and HM) for the preparation of our sensor chips. The medium compositions can be found in the [https://2014.igem.org/Team:Aachen/Notebook/Protocols Protocols] section. We screened for an optimized medium composition to minimize background fluorescence and to enhance cell growth. The results of the analysis are presented in the table below. Due to the low background fluorescence in ''WatsOn'' and the excellent cell growth, we '''chose LB&nbsp;medium''' over the other tested media for sensor chip manufacturing.<br />
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<center><br />
{| class="wikitable"<br />
! !! LB !! TB !! NA !! M9 !! HM <br />
|-<br />
| Growth of ''Cellock'' || <div style="text-align: center;">'''+'''</div> || <div style="text-align: center;">'''+'''</div> || <div style="text-align: center;">-</div> || <div style="text-align: center;">-</div> || <div style="text-align: center;">-</div><br />
|-<br />
| Background fluorescence in GelDoc || <div style="text-align: center;">'''+'''</div> || <div style="text-align: center;">'''+'''</div> || <div style="text-align: center;">-</div> || <div style="text-align: center;">-</div> || <div style="text-align: center;">-</div><br />
|-<br />
| Background fluorescence in ''WatsOn'' || <div style="text-align: center;">-</div> || <div style="text-align: center;">'''+'''</div> || <div style="text-align: center;">-</div> || <div style="text-align: center;">-</div> || <div style="text-align: center;">-</div><br />
|}<br />
</center><br />
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Another set of experiments were conducted to test the '''long-time storage''' of the sensor chips. We varied the glycerol content of the chips as well as the storage temperature. Storage at -20°C resulted in the loss of our sensor cells. Adding 5-10% (v/v) glycerol ensured survival of the sensor cells, but resulted in the loss of fluorescence ability. Hence, we concluded that long-time storage of the sensor chips at -20°C is not possible under the tested conditions. However, the 'ready-to-use' sensor chips can be kept at at 4°C for two days when using LB medium, and storage at this temperature for 5 days is possible with chips made from TB medium.<br />
<!--Regarding the medium used for our sensor chips, LB medium showed a high background fluorescence when exposed to UV light in the Gel Doc. Surprisingly, the background fluorescence resulting from the LB medium was too high to detect a signal emitted by our sensor cells. Hence, minimal media (NA, M9, Hartman (HM)) was used to minimize background fluorescence, but this approach resulted in less to no growth of our sensor cells. In our device ''WatsOn'', optimized wavelengths of 450&nbsp;nm and 480&nbsp;nm were used for excitation of iLOV and GFP, respectively. When exposed to either excitation wavelength TB medium, which is basically an improved LB medium and highly supports cell growth, showed strong background fluorescence in our own device. High background fluorescence was also observed for TB medum when using the Gel Doc. In contrast to the Gel Doc LB medium showed minimal fluorescence in our device ''WatsOn'' and no difficulties in cultivation of our ''Cellocks'' were observed. Because of the reduced fluorescence compared to TB medium when using ''Watson'' for sensor chip evaluation and because of sufficient cultivation conditions for our 'Cellocks'' LB medium was chosen over TB mediium for sensor chip manufacturing. --><br />
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{{Team:Aachen/FigureFloat|Aachen_2_chipform.jpg|title=Sensor chip manufacturing using the closed mold|subtitle=When injecting the liquid agar into a closed mold we encounter problems due to frequent bubble formation.|left|width=500px}}<br />
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=== Optimal Agarose Concentration for Sensor Chip Manufacturing ===<br />
For the sensor chip manufacturing, agarose was preferred over agar because of the uniform linkage between molecules that results in a better chip homogeneity. In addition, agarose reduced diffusion of the inducer molecules through the chip. A reduction in diffusion is essential for the formation of distinct fluorescent spots on the sensor chips.<br />
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{{Team:Aachen/FigureFloat|Aachen_Final_chipform.jpg|title=The finalized chip mold|subtitle=An open casting mold was found to be optimal for sensor chip manufacturing, because this approach was fast, easy to handle and generated a reproducible chip quality.|left|width=500px}}<br />
=== Optimal Chip Configuration ===<br />
Several approaches were tested for the production of agarose-based sensor chips with reproducible quality. The first approach was to cast every sensor chip individually. To achieve a plain chip surface, a requirement for high quality images, we casted the sensor chips between two microscope slides. However, this approach was not adequate because the agar was too liquid and leaked from the microscope slides. In a second approach, we designed a closed mold into which liquid agar is injected using a pipette, but we encountered a high number of bubbles in the resulting chips. Bubbles in the sensor chips interfered with fluorescence evaluation. Finally, we tried an open casting mold. Once solidified, we cut the agar along precast indentations in the casting mold to form the chips. An advantage of the open mold is the ability to simultaneously produce nine sensor chips while the surface tension of the liquid agar ensures a plane chip surface.<br />
=== Induction of the Sensor Chips ===<br />
To test our molecular constructs, we simulated the presence of ''P.&nbsp;aeruginosa'' by using IPTG or 3-oxo-C<sub>12</sub>-HSL. Initial experiments showed that diffusion of the inducers hinder the formation of distinct fluorescent spots. Through this set of experiments we determined that the best compromise between diffusion and spot intensity is an induction volume of 2.0&nbsp;µL for IPTG and 0.2&nbsp;µL for HSL. Furthermore, detection of growing ''P.&nbsp;aeruginosa'' based on secreted HSLs was possible using the [http://parts.igem.org/Part:BBa_K131026 K131026] construct. The experiments for optimizing the induction of our sensor chips are described in more detail in the [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements Achievements] section.<br />
=== Negative Control ===<br />
To ensure that the fluorescence signal resulted from the sensor construct and not from the medium or ''E. coli'' cells themselves, [http://parts.igem.org/Part:BBa_B0015 B0015] in NEB10β cells was used as negative control during sensor chip induction with IPTG, HSL and ''P.&nbsp;aeruginosa''.<br />
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{{Team:Aachen/Figure|Aachen_B0015_IPTG_HSL_Pseudomonas.png|title=B0015 in NEB10β was used as a negativ control|subtitle=Induction with A) 0.2&nbsp;µL of 100&nbsp;mM IPTG, image taken after 2.5&nbsp;h; B) 0.2&nbsp;µL of 500&nbsp;µg/mL 3-oxo-C<sub>12</sub>-HSL, image after 2.5&nbsp;h; C) with five spots of ''Pseudomonas&nbsp;aeruginosa'' liquid culture on the left and one big spot on the right, image taken after 2&nbsp;h.|width=900px}}<br />
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==Achievements==<br />
<span class="anchor" id="biosensorachievements"></span><br />
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We developed and optimized a 2D biosensor, which is able to detect IPTG, 3-oxo-C{{sub|12}}-HSL and living ''Pseudomonas&nbsp;aeruginosa'' cells. <br />
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{{Team:Aachen/FigureFloat|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=Sensor chips based on [http://parts.igem.org/Part:BBa_K1319042 K1319042] were investigated for fluorescence using a plate reader. Blue color indicates the absence of fluorescence, while red color indicates fluorescence. The upper chip was not induced, while the lower chip was induced with IPTG (2.0&nbsp;µL, 100mM).|width=260px}}<br />
=== Testing our Sensor Chips in a Plate Reader ===<br />
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To establish a prove-of-principle for our sensor chip design, we used our construct [http://parts.igem.org/Part:BBa_K1319042 K1319042], an IPTG inducible iLOV. ''E. coli'' cells carrying the this construct were introduced into sensor chips and fluorescence was measured every 15&nbsp;minutes after induction with 2&nbsp;µL of 100&nbsp;mM IPTG. The results are displayed on the left.<br />
We observed a distinct difference in fluorescence between the non-induced chip (top) and the induced chip (bottom). The middle of the bottom chip started to exhibit a clear and visible fluorescence that increased over time and spread outwards. The top chip, however, also showed an increase in the measured fluorescence over time which was primarily due to the leaky promoter and background fluorescence.<br />
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{{Team:Aachen/FigureFloatRight|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=Sensor chips based on [http://parts.igem.org/Part:BBa_K131026 K131026] were investigated for fluorescence using a plate reader. Blue color indicates the absence of fluorescence, while red color indicates fluorescence. The lower chip was induced with with 3-oxo-C{{sub|12}}-HSL (0.2&nbsp;µL, 500 µg/mL).|width=360px}}<br />
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=== Detecting 3-oxo-C{{sub|12}}-HSL with Sensor Chips ===<br />
<br />
In an initial attempt to detect 3-oxo-C{{sub|12}}-HSL, we incorporated the [http://parts.igem.org/Part:BBa_K131026 K131026] construct generated by the 2008 iGEM Team Calgary in our sensor chips. This construct generates a fluorescent signal based on GFP in presence of 3-oxo-C{{sub|12}}-HSL molecules produced by ''P.&nbsp;aeruginosa'' during quorum sensing (Jimenez, Koch, Thompson et al., 2012). First, we tested the construct by direct induction with 3-oxo-C{{sub|12}}-HSL (0.2&nbsp;µL, 500&nbsp;µg/mL). The fluorescence measurement was taken every 15&nbsp;minutes with an excitation wavelength of 496&nbsp;nm and an emission wavelength of 516&nbsp;nm. The results of this test are shown on the right.<br />
<br />
A distinct fluorescence signal was observed on the induced chip (bottom) compared to the non-induced chip (top). <br />
Fluorescence started in the middle of the chip, the point of induction, and then extended outwards while growing in intesnity. The basal level of fluorescence was attributed to leakiness of the promoter and general background fluorescence of growing ''E. coli'' cells. In the induced chip (bottom), the background fluorescence was lower than in the non-induced chip (top) because the signal masked the noise. The difference between the induced and non-induced chips indicates a clear response to the HSL and proofed the ability of our 2D sensor chip design to detect HSLs produced by ''Pseudomonas&nbsp;aeruginosa''.<br />
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{{Team:Aachen/FigureFloat|Aachen_I746909_slower_reduced.gif|title=IPTG-inducible superfolder GFP (I746909) in sensor chips|subtitle=Expression of superfolder GFP ([http://parts.igem.org/Part:BBa_I746909 I746909]) was induced by the addition of IPTG (2&nbsp;µL,&nbsp;100mM) on the right chip. The left chip was not induced.|width=480px}}<br />
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=== Detecting IPTG with Sensor Chips ===<br />
The clip displayed on the left side shows the construct [http://parts.igem.org/Part:BBa_I746909 I746909] from the 2007 iGEM Team Cambridge which expresses super folder GFP under the control of a T7 promoter in combination with our 2D sensor chip technology. The [http://parts.igem.org/Part:BBa_I746909 I746909] construct was introduced into BL21(DE3) cells to make the expression IPTG-inducible since the genome of BL21(De3) contains the T7 RNA Polymerase under the control of a lacI promoter.<br />
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While the left chip does not show visible fluorescence, the right chip exhibits a strong fluorescence signal. This proves the ability of our sensor chip technology to detect IPTG. The fluorescence response is also high enough to be detected and analyzed by our measurement device ''WatsOn''.<br />
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{{Team:Aachen/FigureFloatRight|Aachen_K131026_HSLdetection_slow.gif|title=Detection of 3-oxo-C{{sub|12}}-HSL with K131026|subtitle=0.2&nbsp;µL of 3-oxo-C{{sub|12}}-HSL was placed in the middle of a sensor chip based on [http://parts.igem.org/Part:BBa_K131026 K131026] followed by incubation at 37°C in ''WatsOn''.|width=480px}}<br />
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===Detecting the 3-oxo-C{{sub|12}}-HSL with K131026 in our Sensor Chips using ''WatsOn''===<br />
<br />
The next step towards the final goal of detecting living cells of ''Pseudomonas&nbsp;aeruginosa'' was to reproduce the detection of 3-oxo-C{{sub|12}}-HSL, already established in the plate reader, in our own [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] device. Therefore, we again used ''E. coli'' BL21(DE3) cells carrying [http://parts.igem.org/Part:BBa_K131026 K131026] and induced with 0.2&nbsp;µL 3-oxo-C{{sub|12}}-HSL of a concentration of 500&nbsp;µg/mL. In the clip displayed on the left, the right chip was induced and - as a negative control - the left chip was not induced. Pictures were taken every four&nbsp;minutes.<br />
<br />
The result was a clear replication of the success of the plate reader experiment. The induced chip showed a clear fluorescence response eminating from the center, where the induction with HSL took place. This demonstrated the ability of not only our sensor chip technology but also our measurement device ''WatsOn to successfully'' detect 3-oxo-C{{sub|12}}-HSL.<br />
<br />
<br />
<br />
<br />
<br />
{{Team:Aachen/FigureFloat|Aachen_K131026_Pseudomonas_aeruginosa_detection.gif|title=Detection of ''Pseudomonas&nbsp;aeruginosa'' with K131026|subtitle=Direct detection of ''Pseudomonas&nbsp;aeruginosa'' on sensor chips based on [http://parts.igem.org/Part:BBa_K131026 K131026].|width=480px}}<br />
<br />
<br />
===Detecting ''Pseudomonas&nbsp;aeruginosa'' with K131026 in our Sensor Chip with ''WatsOn''===<br />
<br />
After establishing the successful detection of 3-oxo-C{{sub|12}}-HSLs with our sensor chips the next step was to detect living cells of ''Pseudomonas aeruginosa'' with our measurement device ''WatsOn''. Therefore sensor chips based on [http://parts.igem.org/Part:BBa_K131026 K131026] were prepared and the right chip was induced with 0.2&nbsp;µL of a ''Pseudomonas aeruginosa'' culture while the left chip was not induced (Detection of 3-oxo-C12 HSL with K131026, displayed below). On the induced chip, a clear fluorescence signal was visible in response to ''P. aeruginosa''. The fluorescence signal emerged outward from the induction point and showed a significant difference to the non-induced chip. The results clearly demonstrate the ability of our sensor chip technology and our measurement device ''WatsOn'' to detect ''P. aeruginosa''!<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-16_Outlook_Cellocks_iNB.png|right|150px]]<br />
<br />
== Outlook ==<br />
<span class="anchor" id="biosensoroutlook"></span><br />
<br />
We are '''committed to improve''' our sensor chip platform. The current technique of using a simple agarose chip is not sufficient to collect all microorganisms from the sampled surface. Therefore, the aim is to improve the sampling chip by using a different, more adhesive material. <br />
<br />
Furthermore, diffusion in the sensor chips will be reduced to '''limit the spread of the fluorescence signal'''. Currently, the fluorescence spot grows beyond the point of induction and makes it difficult to differentitate between multiple points of induction. By introducing diffusion barriers into our chips, the growth of the fluorescence spots might be reduced, thus enabling the detection of multiple sources of fluorescence lying close together. <br />
<br />
Additionally, the application of our sensor chips in combination with our ''WatsOn'' device is currently being evaluated for the detection of signals other than fluorescence. '''Detecting bio- and chemiluminescence''' has been identified and will be investigated as an area of potential future application. <br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==References==<br />
<br />
* Waters, C. M., & Bassler, B. L. (2005). QUORUM SENSING: Cell-to-Cell Communication In Bacteria. Annual Review of Cell and Developmental Biology, 21(1), 319-346. Available online at http://www.annualreviews.org/doi/full/10.1146/annurev.cellbio.21.012704.131001.<br />
<br />
* Jimenez, P. N., Koch, G., Thompson, J. A., Xavier, K. B., Cool, R. H., & Quax, W. J. (2012). The Multiple Signaling Systems Regulating Virulence in ''Pseudomonas aeruginosa''. Microbiology and Molecular Biology Reviews, 76(1), 46-65. Available online at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294424/#B63.<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/AchievementsTeam:Aachen/Achievements2014-10-18T03:39:55Z<p>Mosthege: /* Medal Fulfillments */</p>
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<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/Header}}<br />
<br />
=Achievements=<br />
<br />
On this page, we summarized all things we accomplished throughout our project "''Cellock Holmes'' - A Case of Identity", and present the medal criteria we have fulfilled for the iGEM competition 2014:<br />
<br />
<html><br />
<center><br />
<ul class="menusmall-grid" style="width:1040px"><br />
<!-- Overview --><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achmedals" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Medal Fulfillments</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/2/2d/Aachen_14-10-14_Medals_iFG.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achbiosensor" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">Biosensor</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/5a/Aachen_14-10-14_cellock_liegend_panel_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achwatson" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;"><i>WatsOn</i></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c7/Aachen_WatsOn_easy.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
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</a><br />
</li><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achdevice" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:40%;">OD/F Device</div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0f/Aachen_14-10-10_ODF_Button_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Achievements#achinterlab" style="color:black"><br />
<div class="menusmall-item menusmall-info" style="height: 180px; width: 180px;"><div class="menukachel" style="top:32%; line-height:1.5em;">Interlab<br>Study</br></div></div><br />
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/3/39/Aachen_Interlab_Cellocks.png); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"><br />
</div><br />
</a><br />
</li><br />
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</ul><br />
</center><br />
</html><br />
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{{Team:Aachen/BlockSeparator}}<br />
<br />
==Medal Fulfillments==<br />
<span class="anchor" id="achmedals"></span><br />
<br />
{| style="float:left; margin-left:30px; margin-bottom:20px"<br />
| https://static.igem.org/mediawiki/2014/thumb/b/b8/Aachen_14-10-14_Bronze_iFG.png/230px-Aachen_14-10-14_Bronze_iFG.png<br />
|<span style="font-size:150%">'''Bronze Requirements (6/6)'''</span><br />
# Our team was registered successfully.<br />
# The [https://igem.org/2014_Judging_Form?id=1319 Judging form] was completed.<br />
# Team [https://2014.igem.org/Team:Aachen/ Wiki] was created.<br />
# We are looking forward to present a poster and a talk at the iGEM Jamboree.<br />
# We successfully participated in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study].<br />
# Several [https://2014.igem.org/Team:Aachen/Parts parts] were submitted to the [http://parts.igem.org/Main_Page Registry of Standard Biological Parts].<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/4/42/Aachen_14-10-14_Silver_iFG.png/230px-Aachen_14-10-14_Silver_iFG.png<br />
|<span style="font-size:150%;">'''Silver Requirements (4/4)</span><br />
# It was experimentally validated that [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] and Devices of our own design work as expected.<br />
# The characterization of [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] is documented in the 'Main Page' section of that Registry entry and in the parts section of our Wiki.<br />
# [http://parts.igem.org/Part:BBa_K1319001 K1319001], [http://parts.igem.org/Part:BBa_K1319004 K1319004] and other [https://2014.igem.org/Team:Aachen/Parts BioBrick Parts] were submitted to the iGEM Parts Registry.<br />
# During the project we addressed several [https://2014.igem.org/Team:Aachen/PolicyPractices important questions beyond the bench]. Together with students of the [https://2014.igem.org/Team:Aachen/Collaborations/Kaiser-Karls-Gymnasium Kaiser-Karls-Gymnasium] and the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab Gymnasiun am Neandertal] as well as people at the [https://2014.igem.org/Team:Aachen/Collaborations/MakerFaire MakerFaire] and at the [https://2014.igem.org/Team:Aachen/Meetup iGEM Meetup] we discussed several issues regarding our project, microorganisms in our environment and synthetic biology. Since we use genetically engineered bacteria to detect human pathogens, [https://2014.igem.org/Team:Aachen/Safety safety] was an important aspect for us throughout the whole project. Together with other German iGEM Teams we encountered an issue regarding [https://2014.igem.org/Team:Aachen/PolicyPractices/BioBrickIntellectualProperty intellectual property rights] in context of the legal status of Biobricks.<br />
|-<br />
|colspan="2" | &nbsp;<br />
|-<br />
|https://static.igem.org/mediawiki/2014/thumb/5/5b/Aachen_14-10-14_Gold_iFG.png/230px-Aachen_14-10-14_Gold_iFG.png<br />
|<span style="font-size:150%;">'''Gold Requirements (4/1)'''</span><br />
# We demonstrated a substantial improvement in cost efficiency of our [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] in comparison to commercially available spectrophotometers. For more information see [https://2014.igem.org/Team:Aachen/PolicyPractices/Economics Economical View].<br />
# We increased the accessability of our new measurement techniques by sharing instructions to build our DIY devices [https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn#watsondiy ''WatsOn''] and the [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy OD/F Device].<br />
# Helped three other iGEM Teams:<br />
#:* We built a [https://2014.igem.org/Team:Aachen/Collaborations/Braunschweig low-cost DIY methane sensor] for [https://2014.igem.org/Team:Braunschweig Team Braunschweig].<br />
#:* We built [https://2014.igem.org/Team:Aachen/Collaborations/Freiburg AcCELLoMatrix Masks] for [https://2014.igem.org/Team:Freiburg Team Freiburg].<br />
#:* We [https://2014.igem.org/Team:Aachen/Collaborations/Heidelberg tested different expression vectors] constructed by [https://2014.igem.org/Team:Heidelberg Team Heidelberg].<br />
# On top of the Policy & Practice aspects already mentioned in the Silver Medal Requirements, we also critically [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab#neanderlabevaluation evaluated] the effectiveness of our approach to increase the social acceptance of gene technology in general, and of our initiative to spark interest for synthetic biology and DIY hardware.<br />
|}<br />
<br />
In addition to the medal criteria of the measurement track, we have characterized the existing BioBricks [http://parts.igem.org/Part:BBa_K131026 K131026] and [http://parts.igem.org/Part:BBa_I746909 I746909]. We improved [http://parts.igem.org/Part:BBa_K731520 K731520] by swapping the GFP with an iLOV (now [http://parts.igem.org/Part:BBa_K1319042 K1319042]) and showed that it functions in our two-dimensional biosensor [[Team:Aachen/Project/2D_Biosensor#Achievements|link]].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-14_cellock_liegend_panel_iNB.png|right|150px]]<br />
<br />
==Biosensor==<br />
<span class="anchor" id="achbiosensor"></span><br />
<br />
We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] for detecting pathogens using a two dimensional biosensor. In our approach, the sensor cells are immobilized in '''[https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensordevelopment optimized agar chips]'''. It has been '''experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated]''' that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''].<br />
<br />
In order to improve the response of our sensor cells to induction we '''designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system]''' using a TEV protease to activate a reporter protein by cleaving off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by '''[https://2014.igem.org/Team:Aachen/Project/Model computational modeling] as well as in our [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements experiments]'''. All parts needed to build this kind of reporter system have been '''submitted to the parts registry'''.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen WatsOn easy.png|right|150px]]<br />
<br />
==''WatsOn''==<br />
<span class="anchor" id="achwatson"></span><br />
<br />
[https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] is a measuring device designed to read out novel two dimensional biosensor. Using ''WatsOn'', we could demonstrate the '''successful detection''' of ''Pseudomonas&nbsp;aeruginosa'' with our chip technology. This measurement device is built from inexpensive and easily available parts to make it ideal for use in low-budget institutions. Following our economic strategy embracing the Open Access principle, we published all technical details and constructional manuals for ''WatsOn'' on our wiki.<br />
<br />
In addition, we have shown that our '''image analysis software [https://2014.igem.org/Team:Aachen/Project/Measurement_Device#watsonmeasurarty ''Measurarty'']''' can analyze photos of the sensor chips taken by ''WatsOn'' and can '''effectively segment the image''' into pathogenic regions and non-pathogenic regions.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_14-10-10_ODF_Button_ipo.png|right|150px]]<br />
<br />
==OD/F Device==<br />
<span class="anchor" id="achdevice"></span><br />
<br />
In addition to our main project, we built an [https://2014.igem.org/Team:Aachen/OD/F_device OD/F Device] capable of measuring optical density and flourescence. It '''beats commercially available devices in cost and portability'''. It can be built with common, inexpensive and easily available parts. A [https://2014.igem.org/Team:Aachen/Notebook/Engineering/ODF#diy construction manual] is available on our wiki.<br />
<br />
The device has been '''successfully tested''' with one of the target groups, high school students, in our collaboration with the [https://2014.igem.org/Team:Aachen/Collaborations/Neanderlab NEAnderLab].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
[[File:Aachen_Interlab_Cellocks.png|right|150px]]<br />
<br />
==Interlab Study==<br />
<span class="anchor" id="achinterlab"></span><br />
<br />
Since we compete in the Measurement Track of this year's iGEM competition, one of our bronze medal criteria was to participate in the [https://2014.igem.org/Team:Aachen/Interlab_Study Measurement Interlab Study]. Our team was able to measure optical density and fluorescence of ''E.&nbsp;coli'' cells containing the three specified genetic devices, and '''obtained high quality data''' supporting the hypotheses regarding different expression levels depending on plamid copy number and promoter strength.<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/PartsTeam:Aachen/Parts2014-10-18T03:23:07Z<p>Mosthege: /* Constitutive expression of GFP-EYFP fusion protein */</p>
<hr />
<div>__NOTOC__<br />
{{Team:Aachen/Header}}<br />
= iGEM Team Aachen BioBricks =<br />
<br />
This page lists the collection of BioBricks developed by our team for the project ''Cellock Holmes - A Case of Identity''.<br />
<br />
<center><br />
{| class="wikitable"<br />
| [https://2014.igem.org/Team:Aachen/Parts#partsK1319000 K1319000]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319001 K1319001]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319002 K1319002]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319003 K1319003]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319004 K1319004]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319008 K1319008]<br />
|-<br />
| [https://2014.igem.org/Team:Aachen/Parts#partsK1319009 K1319009]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319010 K1319010]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319011 K1319011]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319012 K1319012]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319013 K1319013]<br />
||[https://2014.igem.org/Team:Aachen/Parts#partsK1319014 K1319014]<br />
|-<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319015 K1319015]<br />
|| [https://2014.igem.org/Team:Aachen/Parts#partsK1319016 K1319016]<br />
||[https://2014.igem.org/Team:Aachen/Parts#partsK1319017 K1319017]<br />
||[https://2014.igem.org/Team:Aachen/Parts#partsK1319020 K1319020]<br />
||[https://2014.igem.org/Team:Aachen/Parts#partsK1319042 K1319042]<br />
|} </center><br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319000 K1319000]==<br />
<span class="anchor" id="partsK1319000"></span><br />
<br />
===RFC &#91;25&#93; Version of E0020===<br />
<br />
This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.<br />
<br />
The coding sequence encodes EYFP (enhanced yellow fluorescent protein) which is derived from ''A. victoria'' GFP. The excitation is 512&nbsp;nm and the emission is 534 nm. This part was used to create the parts K1319001 and K1319002. It can also be used in a fusion protein instead of E0030 due to its RFC[25] compability.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
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==[http://parts.igem.org/Part:BBa_K1319001 K1319001]==<br />
<span class="anchor" id="partsK1319001"></span><br />
<br />
===RFC [25] - compatible dark quencher based on K1319000 (E0030) called REACh1===<br />
<br />
This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP) called REACh1.<br />
<br />
Two mutations were introduced that eliminated fluorescence:<br />
* L90I<br />
* Y145W<br />
<br />
===References===<br />
* Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central]<br />
<br />
<br />
===Usage and Biology===<br />
This protein is designed to be a dark quencher for GFP ([http://parts.igem.org/Part:BBa_E0040 E0040]) in a FRET system. When used in a fusion protein with GFP it reduces the observed fluorescence of GFP drastically. In the biobrick [http://parts.igem.org/Part:BBa_K1319013 K1319013] this is realized and the proteins are fused together with the linker [http://parts.igem.org/Part:BBa_K1319016 K1319016] which includes a specific TEV protease (available as [http://parts.igem.org/Part:BBa_K1319004 K1319004]) cleavage site. The fusion of the proteins bring GFP and REACh1 in proximity to each other which allows GFP and REACh1 to act as donors and acceptors in a FRET (Förster Energy Transfer System) system. GFPs emission energy is thereby taken up by REACh1 and released as thermal energy instead of visible light. This eliminates the GFP fluorescence and allows the release of a strong fluorescence signal, if a TEV protease is expressed and the linker is cut. The cutting separates GFP and REACh1 cancelling the FRET interaction and providing a GFP fluorescence response.<br />
<br />
===Characterization===<br />
<br />
In order to characterize K1319001 it was expressed as a fusion protein together with GFP to show its quenching ability. This fusion protein, produced by K1319013, was located on the plasmid backbone pSB3K3. To show that the reduced fluorescence was due to the quenching ability of K1319001 and not based on a faulty expression, K1319008 was also introduced into the cells on a pSB1C3 backbone to create a double plasmid system. Both constructs were put into ''E. coli'' BL21 (DE3) and compared to [http://parts.igem.org/Part:BBa_I20260 I20260] and [http://parts.igem.org/Part:BBa_B0015 B0015]. B0015 was used as a negative control and I20260 was chosen as a positive control due to the expressed GFP being identical to the GFP being expressed in the fusion protein of K1319013 and having the same promoter, RBS, Terminator and plasmid backbone. <br />
<br />
The double plasmid system, B0015 and I20260 were measured in biological triplicates and after 2 h one set of triplicates of each of the three systems were induced with 50 µl 100mM IPTG in their 50 ml culture (500 ml shake flasks). Fluorescence measurement was performed with the [http://www.biotek.com/products/microplate_detection/synergymx_monochromator_based_multimode_microplate_reader.html Synergy Mx from BioTek] with an excitation wavelength of 496 ± 9 nm and emission wavelength of 516 ± 9 nm. The following graph shows the resulting fluorescence adjusted for the measured optical density to account for difference in growth of the cultures and to only show the fluorescence per cell.<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/5/56/Aachen_Graph2_13.PNG" width="800px"></html><br />
|-<br />
|'''{{{title|Comparison of the fluorescence adjusted for OD of I20260, B0015 and the double plasmid system K1319013 + K1319008}}}'''<br />{{{subtitle|After induction with IPTG after 2 h the double plasmid system produced a fast fluorescence response with an over 9-fold increase compared to the non induced state. I20260 served as positive control and B0015 as negative control.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
The strong fluorescence response (9-fold increase) after induction with IPTG shows the functionality of GFP inside the fusion protein. After the induction a TEV protease is produced which is specifically cutting the recognition sequence build inside the linker (K1319016) between GFP and K1319001. The cutting results in a separation of GFP and REACh1 collapsing the FRET system between the two. This results in a fluorescence signal of GFP due to the fact that the emission is no longer absorbed by REACh1 and emitted as heat but rather as fluorescence with a wavelength of 511 nm.<br />
<br />
The very low fluorescence in the non induced double plasmid system of K1319013 and K1319008 shows the functionality of K1319001. As established before, the GFP is being expressed correctly inside the fusion protein, therefore the reduction in fluorescence in the non induced double plasmid system is a direct result of the quenching ability of K1319001. The quenching, after subtraction of the background fluorescence, reduced the fluorescence of GFP by a factor of 12,5. This also includes the slight leakiness of the TEV protease.<br />
<br />
To eliminate the effect of the leakiness of the K1319008 construct in determining the quenching ability of K1319001, K1319013 was also compared against I20260 and B0015 on its own under the same condition as above (again in a biological triplicate).<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/b/bb/Aachen_K1319001_comparison_positive_negative.PNG" width="800px"></html><br />
|-<br />
|'''{{{title|Comparison of the fluorescence adjusted for OD of I20260, B0015 and K1319013}}}'''<br />{{{subtitle|The expressed fusion protein K1319013 exhibits a fluorescence more than 30 fold smaller as the positive control of I20260.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
This experiments shows that the fluorescence of the fusion protein GFP-REACh1 is more than 30-fold lower than normal GFP expression under identical circumstances (same backbone, promoter, RBS, terminator and cultivation circumstances). This demonstrates a quenching percentage of GFP of >96 %!<br />
<br />
To prove that the measured constructs were the same as assumed the plasmids were tested with specially designed Check PCRs with one primer binding upstream on the plasmid backbone and one primer binding specifically inside the insert. The following results were obtained for K1319013.<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/7/74/Aachen_K1319013_Gel_Check_PCR.png" width="500px"></html><br />
|-<br />
|'''{{{title|Check PCR for K1319013}}}'''<br />{{{subtitle|The length of the PCR product matches the length of the control plasmid.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
The PCR clearly shows that the used plasmid had the correct insert. After a positive identification of K1319013 the double plasmid system was also checked for the correct TEV protease plasmid K1319008.<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/0/0c/Aachen_K1319008_Gel_Check_PCR.png" width="500px"></html><br />
|-<br />
|'''{{{title|Check PCR for K1319008}}}'''<br />{{{subtitle|The length of the PCR product matches the length of the control plasmid.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
This PCR also positively identified K1319008. Additionally both parts were sequenced. The sequencing data for [http://parts.igem.org/Part:BBa_K1319008 K1319008] and [http://parts.igem.org/Part:BBa_K1319013 K1319013] can be found in the parts registry.<br />
<br />
===characterization of K1319001 with the iGEM Team Aachen [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor 2D Biosensor]===<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 40px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/6/6d/Aachen_K1319014%2B8_and_K1319013%2B8_pixelig_minus_bg.gif" width="500px"></html><br />
|-<br />
|'''{{{title|K1319013 + K1319008 and K1319014 + K1319008 non-induced (top) and induced (bottom) in sensor cells }}}'''<br />{{{subtitle|The induced double plasmid systems K1319013 + K1319008 and K1319014&nbsp;+&nbsp;K1319008 exhibit a clear fluorescence response in our sensor cells which in response to induction with 2 µl IPTG. }}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
To further characterize the REACh construct, they were introduced into the sensor cells which were then induced with 2&nbsp;µL IPTG with a concentration of 100&nbsp;mM. Subsequently, we took fluorescence measurement read-outs (GFP, excitation 496&nbsp;±&nbsp;9&nbsp;nm, emission&nbsp;516&nbsp;±&nbsp;9&nbsp;nm) roughly every 10&nbsp;min in the plate reader. The results were plotted in the heatmap shown on the left. <br />
<br />
The heatmap shows an increase of fluorescence from blue (no fluorescence) to red (high fluorescence). It is clearly visible that the induced chips are exhibiting a significantly higher fluorescence than the non-induced chips. This again shows that the constructs work as intended: The TEV protease cuts the linker so that the fusion protein is separated into GFP and a dark quencher, disabling the quenching. GFP has a clear fluorescence emission after the fusion protein has been successfully cut into two pieces by the TEV protease. <br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319002 K1319002]==<br />
<span class="anchor" id="partsK1319002"></span><br />
<br />
===RFC [25] - compatible dark quencher based on K1319000 (E0030) called REACh2===<br />
<br />
This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP) called REACh2. <br />
<br />
Three mutations were introduced that eliminated fluorescence: <br />
* L90I <br />
* Y145W <br />
* H148R<br />
<br />
===References===<br />
* Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central]<br />
<br />
===Usage and Biology===<br />
This protein is designed to be a dark quencher for GFP ([http://parts.igem.org/Part:BBa_E0040 E0040]) in a FRET system. When used in a fusion protein with GFP it reduces the observed fluorescence of GFP drastically. In the biobrick [http://parts.igem.org/Part:BBa_K1319014 K1319014] this is realized and the proteins are fused with the linker [http://parts.igem.org/Part:BBa_K1319016 K1319016] which includes a specific TEV protease (available as [http://parts.igem.org/Part:BBa_K1319004 K1319004]) cleavage site. The fusion of the proteins brings GFP and REACh2 in proximity to each other which allows GFP and REACh2 to act as donors and acceptors in a FRET (Förster Energy Transfer System) system. GFPs emission energy is thereby taken up by REACh2 and released as thermal energy instead of visible light. This eliminates the GFP fluorescence and allows a release of a strong fluorescence signal if a TEV protease is expressed and the linker is cut. The cutting separates GFP and REACh2 cancelling the FRET interaction and providing a GFP fluorescence response.<br />
<br />
===Characterization===<br />
<br />
In order to characterize K1319002 it was expressed as a fusion protein together with GFP to show its quenching ability. This fusion protein, produced by K1319014, was located on the plasmid backbone pSB3K3. To show that the reduced fluorescence was due to the quenching ability of K1319002 and not based on a faulty expression, K1319008 was also introduced into the cells on a pSB1C3 backbone to allow for a double plasmid system. Both constructs were put into ''E. coli'' BL21 (DE3) and compared to [http://parts.igem.org/Part:BBa_I20260 I20260] and [http://parts.igem.org/Part:BBa_B0015 B0015]. B0015 was used as a negative control and I20260 was chosen as a positive control due to the expressed GFP being identical to the GFP being expressed in the fusion protein of K1319014 and having the same promoter, RBS, Terminator and plasmid backbone. <br />
<br />
The double plasmid system, B0015 and I20260 were measured in biological triplicates and after 2 h one set of triplicates of each of the three systems were induced with 50 µl 100mM IPTG in their 50 ml culture (500 ml shake flasks). Fluorescence measurement was performed with the [http://www.biotek.com/products/microplate_detection/synergymx_monochromator_based_multimode_microplate_reader.html Synergy Mx from BioTek] with an excitation wavelength of 496 ± 9 nm and emission wavelength of 516 ± 9 nm. The following graph shows the resulting fluorescence adjusted for the measured optical density to account for difference in growth of the cultures and to only show the fluorescence per cell.<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/3/3e/Aachen_Graph2_14.PNG" width="800px"></html><br />
|-<br />
|'''{{{title|Comparison of the fluorescence adjusted for OD of I20260, B0015 and the double plasmid system K1319014 + K1319008}}}'''<br />{{{subtitle|After induction with IPTG after 2 h the double plasmid system produced a fast fluorescence response with an over 10-fold increase compared to the non induced state. I20260 served as positive control and B0015 as negative control.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
The strong fluorescence response (10-fold increase) after induction with IPTG shows the functionality of GFP inside the fusion protein. After the induction a TEV protease is produced which is specifically cutting the recognition sequence build inside the linker (K1319016) between GFP and K1319002. The cutting results in a separation of GFP and REACh2 resulting in a collapse of the FRET system between the two. This results in a fluorescence signal of GFP due to the fact that the emission is no longer absorbed by REACh2 and emitted as heat but rather as fluorescence with a wavelength of 511 nm. The overall fluorescence of the double plasmid system reaches the fluorescence level of the positive control indicating a total clavage of all fusion proteins by the produced TEV protease. <br />
<br />
The very low fluorescence in the non induced double plasmid system of K1319014 and K1319008 shows the functionality of K1319002. As established before, the GFP is being expressed correctly inside the fusion protein, therefore the reduction in fluorescence in the non induced double plasmid system is a direct result of the quenching ability of K1319002. The quenching, after subtraction of the background fluorescence, reduced the fluorescence of GFP by a factor of 10. This also includes the slight leakiness of the TEV protease.<br />
<br />
To eliminate the effect of the leakiness of the K1319008 construct in determining the quenching ability of K1319002, K1319014 was also compared against I20260 and B0015 on its own under the same condition as above (again in a biological triplicate).<br />
<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/6/61/Aachen_K1319002_characterization_positive_negative.PNG" width="800px"></html><br />
|-<br />
|'''{{{title|Comparison of the fluorescence adjusted for OD of I20260, B0015 and K1319014}}}'''<br />{{{subtitle|The expressed fusion protein K1319014 exhibits a fluorescence more than nearly 25-fold smaller as the positive control of I20260.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
This experiments shows that the fluorescence of the fusion protein GFP-REACh2 is nearly 25-fold lower than normal GFP expression under identical circumstances (same backbone, promoter, RBS, terminator and cultivation circumstances). This demonstrates a quenching percentage of GFP of ~96 %!<br />
<br />
To prove that the measured constructs were the same as assumed the plasmids were tested with specially designed Check PCRs with one primer binding upstream on the plasmid backbone and one primer binding specifically inside the insert. The following results were obtained for K1319014.<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/0/08/Aachen_K1319014_Gel_Check_PCR.png" width="500px"></html><br />
|-<br />
|'''{{{title|Check PCR for K1319014}}}'''<br />{{{subtitle|The length of the PCR product matches the length of the control plasmid.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
The PCR clearly shows that the used plasmid had the correct insert. After a positive identification of K1319014 the double plasmid system was also checked for the correct TEV protease plasmid K1319008.<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/0/0c/Aachen_K1319008_Gel_Check_PCR.png" width="500px"></html><br />
|-<br />
|'''{{{title|Check PCR for K1319008}}}'''<br />{{{subtitle|The length of the PCR product matches the length of the control plasmid.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
This PCR also positively identified K1319008. Additionally both parts were sequenced. The sequencing data for [http://parts.igem.org/Part:BBa_K1319008 K1319008] and [http://parts.igem.org/Part:BBa_K1319014 K1319014] can be found in the parts registry.<br />
<br />
===characterization of K1319001 with the iGEM Team Aachen [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor 2D Biosensor]===<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 40px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/6/6d/Aachen_K1319014%2B8_and_K1319013%2B8_pixelig_minus_bg.gif" width="500px"></html><br />
|-<br />
|'''{{{title|K1319013 + K1319008 and K1319014 + K1319008 non-induced (top) and induced (bottom) in sensor cells }}}'''<br />{{{subtitle|The induced double plasmid systems K1319013 + K1319008 and K1319014&nbsp;+&nbsp;K1319008 exhibit a clear fluorescence response in our sensor cells which in response to induction with 2 µl IPTG. }}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
To further characterize the REACh construct, they were introduced into the sensor cells which were then induced with 2&nbsp;µL IPTG with a concentration of 100&nbsp;mM. Subsequently, we took fluorescence measurement read-outs (GFP, excitation 496&nbsp;±&nbsp;9&nbsp;nm, emission&nbsp;516&nbsp;±&nbsp;9&nbsp;nm) roughly every 10&nbsp;min in the plate reader. The results were plotted in the heatmap shown on the left. <br />
<br />
The heatmap shows an increase of fluorescence from blue (no fluorescence) to red (high fluorescence). It is clearly visible that the induced chips are exhibiting a significantly higher fluorescence than the non-induced chips. This again shows that the constructs work as intended: The TEV protease cuts the linker so that the fusion protein is separated into GFP and a dark quencher, disabling the quenching. GFP has a clear fluorescence emission after the fusion protein has been successfully cut into two pieces by the TEV protease. <br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319003 K1319003]==<br />
<span class="anchor" id="partsK1319003"></span><br />
<br />
===human galectin-3, codon optimized for ''E. coli''===<br />
<br />
Galectin-3 is a 26 kDa protein that binds certain LPS patterns. It especially bind the O-section of the LPS.<br />
<br />
Galectins are proteins of the lectin family, which posess '''carbonhydrate recognition domains''' binding specifically to β-galactoside sugar residues. In humans, 10 different galectines have been identified, among which is galectin-3. <br />
<br />
Galectin-3 has a size of about 31&nbsp;kDA and is encoded by a single gene, LGALS3. It has many physiological functions, such as '''cell adhesion, cell growth and differentiation,''' and contributes to the development of '''cancer, inflammation, fibrosis and others'''.<br />
<br />
Human galectin-3 is a protein of the lectin-family that was shown to bind the LPS of multiple human pathogens.<br />
Some of them, including ''Pseudomonas&nbsp;aeruginosa'' protect themselves against the human immune system by mimicking the lipopolysaccharides (LPS) present on human erythrocytes. <br />
<br />
===Usage and Biology===<br />
<br />
K1319003 was used to create [http://parts.igem.org/Part:BBa_K1319020 K1319020], a Galectin-mRFP fusion protein with a C-terminal His tag in the [https://2014.igem.org/Team:Heidelberg/Team/Collaborations Heidelberger expression vector] pSBX1A3.<br />
<br />
We also cloned our K1319003 into the pET17 expression vector and expressed all combinations of fusion proteins in E.&nbsp;coli&nbsp;BL21(DE3). An SDS-PAGE showed that all fusion proteins were fully translated:<br />
<center><br />
<div class="figure" style="float:{{{align|center}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/thumb/5/52/Aachen_14-10-04_Expression_Pellets_iMO.png/425px-Aachen_14-10-04_Expression_Pellets_iMO.png" width="400px"></html><br />
|-<br />
|'''{{{title|Pellets of different fusion protein expressions}}}'''<br />{{{subtitle|Expression in the pET17 vector was much more leaky than the expression in the pSBX vectors.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<center><br />
<div class="figure" style="float:{{{align|center}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/thumb/6/62/Aachen_Gal3_Expression.png/425px-Aachen_Gal3_Expression.png" width="400px"></html><br />
|-<br />
|'''{{{title|SDS-PAGE of K1319020 expression}}}'''<br />{{{subtitle|The fusion protein was fully translated to the correct molecular mass of 74&nbsp;kDa.}}}<br />
|}<br />
</div><br />
</center><br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319004 K1319004]==<br />
<span class="anchor" id="partsK1319004"></span><br />
<br />
===TEV protease with anti-self cleavage mutation S219V, codon optimized for ''E. coli''===<br />
<br />
This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.<br />
<br />
The TEV Protease (also known as Tobaco Edge Virus nuclear inclusion a endopeptidase) is a highly sequence specific cysteine protease from the Tobacco Edge Virus (TEV). The protease is highly sequence specific. The consensus sequence for the cut is ENLYFQ\S with \ denoting the cleaved peptide bond. This sequence can be found in the part [http://parts.igem.org/Part:BBa_K1319016 K1319016]. <br />
<br />
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.<br />
<br />
The TEV protease is commonly used as a biochemical tool to cleave affinity tags from purified proteins like [http://parts.igem.org/Part:BBa_K1319007 His-Tags]. The high specifity makes the protease relatively non-toxic ''in vitro'' and ''in vivo''. The molecular weight of the TEV protease is 27 kDa.<br />
<br />
===Usage and Biology===<br />
<br />
The TEV Protease was used and characterizes in the [http://parts.igem.org/Part:BBa_K1319008 K1319008] construct.<br />
<br />
To characterize the TEV protease we used the fusion protein [http://parts.igem.org/Part:BBa_K1319014 K1319014]. This fusion protein contains GFP ([http://parts.igem.org/Part:BBa_E0040 E0040]) bound to a dark quencher ([http://parts.igem.org/Part:BBa_K1319002 REACh2/K1319002]) over a [http://parts.igem.org/Part:BBa_K1319016 linker] which includes the TEV protease cleavage site. If the TEV protease successfully cuts the linker, GFP and its quencher would separate and the FRET (Förster Resonance Energy Transfer) system would be shut down. This would result in an increased GFP fluorescence.<br />
<br />
To demonstrate this behaviour a double plasmid system was designed using the biobrick K1319013 in a pSB3K3 backbone and K1319008 in a pSB1C3 backbone. Also [http://parts.igem.org/Part:BBa_I20260 I20260] was used as a positive control because it produces the same GFP as used in the fusion protein and is regulated by the same promoter, RBS and Terminator on the same plasmid backbone. [http://parts.igem.org/Part:BBa_B0015 B0015] was used as negative control. Induction of the double plasmid constructs occured after 2 h with 50 µl of 100mM IPTG in a 50 ml shake flask culture. <br />
<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|800px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/3/3e/Aachen_Graph2_14.PNG" width="800px"></html><br />
|-<br />
|'''{{{title|Comparison of the fluorescence adjusted for OD of I20260, B0015 and the double plasmid system K1319014 + K1319008}}}'''<br />{{{subtitle|After induction with IPTG after 2 h the double plasmid system produced a fast fluorescence response with an over 10-fold increase compared to the non induced state. I20260 served as positive control and B0015 as negative control.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
The increase in fluorescence after induction with IPTG is clear sign of funtional expression of the TEV protease. The difference between not induced and induced plasmid is proof that the increase in fluorescence is only attributed to the successful cleavage of the linker. Therefore this is proof of a functional expression of the TEV protease after induction with IPTG.<br />
<br />
Furthermore we validated the used plasmid with a Check PCR with one primer binding upstream on the plasmid backbone and one specifically in the insert.<br />
<br />
<center><br />
<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|500px}}};padding:10px 10px 0px 0px;"><br />
{|<br />
|<html> <img src="https://static.igem.org/mediawiki/2014/0/0c/Aachen_K1319008_Gel_Check_PCR.png" width="500px"></html><br />
|-<br />
|'''{{{title|Check PCR for K1319008}}}'''<br />{{{subtitle|The length of the PCR product matches the length of the control plasmid.}}}<br />
|}<br />
</div><br />
</center><br />
<html></br></html><br />
<br />
This validates that the construct is indeed the TEV protease and thereby the functionality of the TEV protease is established. The construct K1319008 was also sequenced. The sequencing data can be seen in the parts registry [http://parts.igem.org/Part:BBa_K1319008 here].<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319008 K1319008]==<br />
<span class="anchor" id="partsK1319008"></span><br />
<br />
=== IPTG-induced and T7-driven expression of TEV protease ===<br />
<br />
This protein generator produces TEV protease when induced with IPTG in a DE3 strain.<br />
<br />
===Usage and Biology===<br />
More information about the characterization of this part is available on the parts registry page for [http://parts.igem.org/Part:BBa_K1319004 K1319004]. This part was alos used in the validation and characterization of the parts [http://parts.igem.org/Part:BBa_K1319001 K1319001] and [http://parts.igem.org/Part:BBa_K1319001 K1319001].<br />
<br />
This biobrick is used in our [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh construct].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319009 K1319009]==<br />
<br />
===mRFP-gal3-his fusion protein CDS===<br />
<span class="anchor" id="partsK1319009"></span><br />
<br />
This is a fusion protein created from E1010, K1319003 and K1319007.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319010 K1319010]==<br />
<span class="anchor" id="partsK1319010"></span><br />
<br />
=== Constitutive expression of K1319000 ===<br />
<br />
This part expresses [http://parts.igem.org/Part:BBa_K1319000 K1319000] behind a [http://parts.igem.org/Part:BBa_J23101 J23101] constitutive promoter.<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319011 K1319011]==<br />
<span class="anchor" id="partsK1319011"></span><br />
<br />
=== Constitutive expression of K1319001 ===<br />
<br />
This part expresses [http://parts.igem.org/Part:BBa_K1319001 K1319001] behind a [http://parts.igem.org/Part:BBa_J23101 J23101] constitutive promoter.<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
== [http://parts.igem.org/Part:BBa_K1319012 K1319012] ==<br />
<span class="anchor" id="partsK1319012"></span><br />
<br />
=== Constitutive expression of K1319002 ===<br />
<br />
This part expresses [http://parts.igem.org/Part:BBa_K1319002 K1319002] behind a [http://parts.igem.org/Part:BBa_J23101 J23101] constitutive promoter.<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
== [http://parts.igem.org/Part:BBa_K1319013 K1319013] ==<br />
<span class="anchor" id="partsK1319013"></span><br />
<br />
=== Constitutive expression of GFP-REACh1 fusion protein ===<br />
<br />
This part expresses a [http://parts.igem.org/Part:BBa_E0040 E0040].[http://parts.igem.org/Part:BBa_K1319001 K1319001] fusion protein (GFP-REACh1) behind a [http://parts.igem.org/Part:BBa_J23101 J23101] promoter. <br />
<br />
===Usage and Biology===<br />
More information about the characterization of this part can be found on the part page of [http://parts.igem.org/Part:BBa_K1319001 K1319001].<br />
<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
== [http://parts.igem.org/Part:BBa_K1319014 K1319014] ==<br />
<span class="anchor" id="partsK1319014"></span><br />
<br />
===Constitutive expression of GFP-REACh2 fusion protein===<br />
<br />
This part expresses a [http://parts.igem.org/Part:BBa_E0040 E0040].[http://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein (GFP-REACh1) behind a [http://parts.igem.org/Part:BBa_J23101 J23101] promoter. <br />
[http://parts.igem.org/Part:BBa_K1319002 K1319002] is a '''dark quencher''' that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.<br />
<br />
===Usage and Biology===<br />
The two domains can be separated from each other via a TEV protease cleavage site in the linker.<br />
<br />
More information about the characterization of this part can be found on the part page of [http://parts.igem.org/Part:BBa_K1319002 K1319002].<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
== [http://parts.igem.org/Part:BBa_K1319015 K1319015] ==<br />
<span class="anchor" id="partsK1319015"></span><br />
<br />
===Constitutive expression of GFP-EYFP fusion protein===<br />
<br />
This part expresses a [http://parts.igem.org/Part:BBa_E0040 E0040].[http://parts.igem.org/Part:BBa_K1319000 K1319000] fusion protein (GFP-EYFP) behind a [http://parts.igem.org/Part:BBa_J23101 J23101] promoter.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
== [http://parts.igem.org/Part:BBa_K1319016 K1319016] ==<br />
<span class="anchor" id="partsK1319016"></span><br />
<br />
===TEV protease cleavage site===<br />
<br />
This sequence codes for a [http://parts.igem.org/Part:BBa_K1319004 TEV protease] cleavage site.<br />
<br />
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.<br />
<br />
===Usage and Biology===<br />
<br />
A TEV protease is available codon optimised for ''E. coli'' with the part [http://parts.igem.org/Part:BBa_K1319004 K1319004].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319017 K1319017]==<br />
<span class="anchor" id="partsK1319017"></span><br />
<br />
=== LasI induced iLOV ===<br />
<br />
This device produces iLOV (K660004) in response to a quorum sensing input.<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319020 K1319020]==<br />
<span class="anchor" id="partsK1319020"></span><br />
<br />
===Translational unit of mRFP-galectin3-His===<br />
<br />
This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015)<br />
<br />
===Usage and Biology===<br />
<br />
For more information about the characterization of this part check out [http://parts.igem.org/Part:BBa_K1319003 K1319003].<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
==[http://parts.igem.org/Part:BBa_K1319042 K1319042]==<br />
<span class="anchor" id="partsK1319042"></span><br />
<br />
===IPTG inducible iLOV===<br />
<br />
This part can be used for IPTG-induced expression of K660004 (iLOV).<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
===iGEM Team Aachen Biobrick overview===<br />
<br />
<groupparts>iGEM14 Aachen</groupparts><br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Notebook/SoftwareTeam:Aachen/Notebook/Software2014-10-18T03:05:40Z<p>Mosthege: /* Standalone Software */</p>
<hr />
<div>__NOTOC__<br />
{{Team:Aachen/Header}}<br />
{{Team:Aachen/Stylesheet}}<br />
<br />
= Standalone Software = <br />
In this section of our notebook, we provide you with details about our three standalone applications, ''Measurearty'', ''Serial&nbsp;Client'' and ''Timeseries&nbsp;Labeller''. We have developed these programs for different aspects of our project and used them in combination with electrical [[Team:Aachen/Notebook/Engineering|hardware]] and [[Team:Aachen/Project/2D_Biosensor#Achievements|biological experiments]]. However, they are very versatile and may be used for completely different purposes.<br />
<br />
<br />
<html><br />
<center><br />
<ul class="team-grid" style="width: inherit;"><br />
<!-- Overview --><br />
<br />
<li><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Software/Measurarty" style="color:black"><br />
<div class="team-item team-info" ><div class="menukachel" style="top:44%;">Measurarty</div></div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/2/21/Aachen_14-10-16_Measurarty_button_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="margin-left:40px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Software/Serial Client" style="color:black"><br />
<div class="team-item team-info" ><div class="menukachel" style="top:44%;">Serial Client</div></div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b2/Aachen_SerialClient_icon_V2_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
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<li style="margin-left:40px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Software/TimeseriesLabeller" style="color:black"><br />
<div class="team-item team-info" ><div class="menukachel" style="top:44%;">Timeseries Labeller</div></div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/f/f7/Aachen_17-10-14_toolbox_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
<br />
<br />
</ul><br />
</center><br />
</html><br />
<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Notebook/SoftwareTeam:Aachen/Notebook/Software2014-10-18T03:04:57Z<p>Mosthege: /* Standalone Software */</p>
<hr />
<div>__NOTOC__<br />
{{Team:Aachen/Header}}<br />
{{Team:Aachen/Stylesheet}}<br />
<br />
= Standalone Software = <br />
In this section of our notebook, we provide you with details about our three standalone applications, ''Measurearty'', ''Serial&nbsp;Client'' and ''Timeseries&nbsp;Labeller''. We have developed these programs for different aspects of our project and used them in combination with electrical [[Team:Aachen/Notebook/Engineering|hardware]] and [[Team:Aachen/Project/2D_Biosensor#Achievements|biological experiments]]. However, they are very versatile and may be used for completely different purposes.<br />
<br />
<br />
<html><br />
<center><br />
<ul class="team-grid" style="width: inherit;"><br />
<!-- Overview --><br />
<br />
<li><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Software/Measurarty" style="color:black"><br />
<div class="team-item team-info" ><div class="menukachel" style="top:44%;">Measurarty</div></div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/2/21/Aachen_14-10-16_Measurarty_button_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="margin-left:40px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Software/Serial Client" style="color:black"><br />
<div class="team-item team-info" ><div class="menukachel" style="top:44%;">Serial Client</div></div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b2/Aachen_SerialClient_icon_V2_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
<br />
<li style="margin-left:40px;"><br />
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Notebook/Software/TimeseriesLabeller" style="color:black"><br />
<div class="team-item team-info" ><div class="menukachel" style="top:44%;">Timeseries Labeller</div></div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/f/f7/Aachen_17-10-14_toolbox_ipo.png); norepeat scroll 0% 0% transparent; background-size:100%"><br />
</div><br />
</a><br />
</li><br />
<br />
<br />
</ul><br />
</center><br />
</html><br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/AttributionsTeam:Aachen/Attributions2014-10-18T03:04:03Z<p>Mosthege: /* Attribution of Scientific and General Support */</p>
<hr />
<div>__NOTOC__<br />
{{CSS/Main}}<br />
{{Team:Aachen/Stylesheet}}<br />
{{Team:Aachen/Header}}<br />
<!--<br />
# General Support<br />
# Fundraising help and advice<br />
# Partners<br />
# Project support and advice<br />
# Lab support<br />
# Difficult technique support<br />
# Project advisor support<br />
# Wiki support<br />
# Presentation coaching<br />
# Policy & Practices support<br />
# Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.<br />
--><br />
=Members=<br />
<span class="anchor" id="members"></span><br />
<br />
A core concept of iGEM is the collaboration within an interdisciplinary student team. Accordingly, students of different age, gender and field of study came together to found our team in Aachen, in order to realize our project with joint forces and enthusiasm for the overall goal. Working closely together, we therefore not only have the chance to learn from each other, but also to excel ourselves. Using our collective creativity and common responsibility, we will create something special in order to make our contribution to synthetic biology.<br />
<br />
<center><br />
<html><ul class="team-grid" style="width:1040px"><br />
<!-- team member profiles --><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Mosthege" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Michael Osthege </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Man for Everything<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/35/Aachen_team_member_Michael_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Fgohr" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Florian Gohr </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
King Gibson<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:AZimmermann" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Arne Zimmermann </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
The Patience itself<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Nbailly" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Nina Bailly </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Shake Flask Prep Princess<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:VeraA" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Vera Alexandrova </b><br />
<br/><br/><br />
<i> Biology </i><br />
<br/><br/><br />
Goddess of Plasmid Prep<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Pdemling" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Philipp Demling </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Prince of bad Puns<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:R.hanke" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> René Hanke </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Creative Mind and Cook<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:PatrickO" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Patrick Opdenstein </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Graphics God<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/1/1a/Aachen_team_member_Patrick_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Bpeeters" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Björn Peeters </b><br />
<br/><br/><br />
<i> Computational Engineering Science </i><br />
<br/><br/><br />
Hardware Master<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/8d/Aachen_team_member_Bjoern_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Eshani.sood" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Eshani Sood </b><br />
<br/><br/><br />
<i> Biomedical Engineering </i><br />
<br/><br/><br />
Sunshine<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/52/Aachen_team_member_Eshani_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Mjoppich" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Markus Joppich </b><br />
<br/><br/><br />
<i> Computer Science </i><br />
<br/><br/><br />
Software Expert and Travel Assistant<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/83/Aachen_team_member_Markus_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Ansgar" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Ansgar Niemöller </b><br />
<br/><br/><br />
<i> Computational Engineering Science </i><br />
<br/><br/><br />
GUI Master<br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/4e/Aachen_team_member_Ansgar_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:J.plum" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Julia Plum </b><br />
<br/><br/><br />
<i> Biology and Business Administration </i><br />
<br/><br/><br />
Fundraising and Balancing<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c9/Aachen_team_member_Julia_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
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<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
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<b> Stefan Reinhold </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Treasurer<br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0c/Aachen_team_member_Stefan_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
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<a href="https://2014.igem.org/User:Aschechtel" style="color:black"><br />
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<b> Anna Schechtel </b><br />
<br/><br/><br />
<i> Biology </i><br />
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Chip Queen<br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/a/a4/Aachen_team_member_Anna_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<br />
= Advisors =<br />
<br />
<html><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/5/57/Aachen_team_member_Suresh_01.jpg" width="200px" /></html><br />
<br />
'''Dr.-Ing. Suresh Sudarsan'''<br />
<br />
I'm working as a post-doc with Prof. Blank at RWTH Aachen University. In my Ph.D. I worked under the guidance of Prof. Andreas Schmid (2008-2012) at TU Dortmund, and Prof. Matthias Reuss (2009-2011) at the University of Stuttgart, with a focus on elucidating the link between the central and aromatic metabolism of ''P. putida'' using a systems biology approach.<br />
<br />
I'm fascinated about understanding the collective behavior of microorganisms and their metabolic potential in different niches. In my research, I use tools in ''metabolic engineering & systems biology'' such as metabolomics, fast sampling, kinetic/dynamic modeling and metabolic flux analysis. <br />
<br />
In iGEM, I enjoy working with a group of young scientists from Aachen to achieve our common goal of detecting ''Pseudomonas aeruginosa'' on hard surfaces. Besides science, I try to get myself involved in adventurous sports...but honestly... I just like to relax my day with a delicious meal and a good sci-fi movie!<br />
<br />
<br />
<br />
<html><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/c/c8/Aachen_team_member_Ljubica.jpg" width="200px" /></html><br />
<br />
'''Dr. rer. nat. Ljubica Vojcic'''<br />
<br />
I am working as a Subgroup Leader in Prof. Schwaneberg research group at the Institute of Biotechnology, RWTH Aachen. The research area of the Schwaneberg group focuses on directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequences of mutational biases on the protein level and developing novel high-throughput screening systems that will lead to improved biocatalysts for prominent applications in industry. In particular, my core expertise is development of high throughput screening systems for different enzyme classes in order to redefine the screening step no longer as bottleneck in directed evolution. <br />
<br />
In iGEM, I enjoyed very much to work with highly motivated and ambitious young scientists from RWTH Aachen University. I truly believe that our collaboration has just started and that we will enjoy jointly solving the scientific challenges in the near future. <br />
<br />
<br />
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<br />
=Instructors=<br />
<span class="anchor" id="instructors"></span><br />
== Prof. Dr.-Ing. Lars M. Blank ==<br />
==== RWTH Institute for Applied Microbiology (iAMB) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/4/42/Aachen_LarsBlank.jpg" width="150px" /></html> <br />
Prof. Blank focuses his research on fundamental and applied aspects of microbial metabolism. Of specific interest is the interaction between the metabolic network and the introduced genetic and environmental perturbations. The research on in silico/in vivo metabolic network operations is aimed at a deeper understanding of cell function, with the ultimate goal of rational cell engineering.<br />
<br />
In his teaching, Prof. Blank focuses on the integration of biological concepts with the tools from bioinformatics and engineering. He believes that a sound knowledge base in life sciences is the key for creative and thus successful work in the areas of Metabolic Engineering and Synthetic Biology. Read more about Prof. Blank's work on the [http://www.iamb.rwth-aachen.de/html/members.php?s=det&id=3 iAMB's website].<br />
<html><br><br><br></p></html><br />
<br />
== Prof. Dr. Wolfgang Wiechert ==<br />
==== Forschungszentrum Jülich, Institute of Bio- and Geosciences (IGB-1) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/8/88/Aachen_WolfgangWiechert.jpg" width="150px" /></html> <br />
Prof. Wiechert's main area of work lies within the field of applied systems bio(techno)logy of microorganisms with a special focus on methodological developments for quantitative biology. Characteristics of his research work are a close integration of experimental and theoretical work within multi disciplinary projects. As the head of the Systems Biotechnology research division at Forschungszentrum Jülich, he is developing methods for quantitative metabolomics, fluxomics and proteomics including model based mathematical methods for experimental design, parameter estimation, and process optimization in biotechnological systems. Future work will also incorporate micro fluidic methods for single cell analysis. In general, all research results are used to drive forward the process of gaining knowledge in the course of an iterative improvement of industrial production systems. This proceeds in close cooperation of all working groups at the IBG-1. Together with industrial partners also diverse examples from industry are investigated and further developed. Read more about Prof. Wiechert's work on the [http://www.fz-juelich.de/SharedDocs/Personen/IBG/IBG-1/EN/Research_groups/general/wiechert.html/ IBG-1 website].<br />
<html><br><br><br></p></html><br />
<br />
== Prof. Dr. Ulrich Schwaneberg ==<br />
==== RWTH Institute for Biotechnology, Leibniz Institute for Interactive Materials (DWI) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/f/fe/Aachen_UlrichSchwaneberg.jpg" width="150px" /></html> <br />
The Schwaneberg Group seeks to be at the research frontier in the interdisciplinary field of directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequence of mutational biases on the protein level and developing novel high-throughput screening systems that will ultimately lead to tailored-biocatalysts for significant applications in industry. They train students in the cutting edge technologies of laboratory evolution, biocatalyst engineering and high throughput screening methodologies. The Schwaneberg Group believes in integrating fundamental principles of protein design with environmental awareness in their research and seeks to promote international scientific collaborations. Read more about Prof. Schwaneberg and his work on the [http://www.biotec.rwth-aachen.de/ Schwaneberg Group's website].<br />
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<br />
This project would not have been possible without the generous help and advice from many great people and organizations. We thank all the partners listed below for their essential contributions and financial support that covered registration fees, travel costs, the organization of the meetup in September, laboratory materials and expenses for services and materials.<br />
<br />
=Partners=<br />
<span class="anchor" id="partners"></span><br />
<div align="center"><br />
{|cellpadding="25"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|159px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|318px|link=http://www.niersverband.de/|Niersverband]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Genscript_Logo.png|240px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|193px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|256px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="35"<br />
|[[File:Aachen_bmbf.jpg|326px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|217px|link=http://www.idt-biologika.de/|IDT]]<br />
|}<br />
<br />
{|cellpadding="25"<br />
|[[File:M2p_labs_logo.jpg|103px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|109px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|121px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="25"<br />
|[[File:Aachen_Logo_iAMB.png|229px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|229px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|246px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|}<br />
{|cellpadding="35"<br />
|[[File:Aachen_Logo_ABBt.png|209px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|239px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|209px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|150px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
=Attribution of Scientific and General Support=<br />
<span class="anchor" id="support"></span><br />
Great projects do not only depend on solid funding, but even more on the invaluable support by great people. We found great people not only in our 'home institute' the iAMB, but across many partner institutions.<br />
<br />
<br />
* '''RWTH Institute of Molecular Biotechnology''' (Biology VII)<br />
** Dr. Ulrich Commandeur ''for giving us access to essential resources of the bio7''<br />
** Christina Dickmeis M.Sc. ''who answered lots of questions''<br />
* '''RWTH Institute of Biotechnology''' (Biology VI)<br />
** David Schönauer and Alan Mertens M.Sc. ''who helped us to purify proteins''<br />
* '''Helmholtz Institute for Biomedical Engineering'''<br />
** Prof. Dr. Lothar Elling and Sophia Böcker, M.Sc. ''for giving us access to their gal-3 expression plasmid''<br />
* '''RWTH Institute of Applied Microbiology''' (iAMB)<br />
** Prof. Dr. Miriam Agler-Rosenbaum ''for scientific information on quorum sensing''<br />
** Dr. Martin Zimmermann ''who counseled us on balancing iGEM with our studies''<br />
** Dr. Andrea Germer ''for teaching us Gibson cloning''<br />
** Erick Bosire Maosa M.Sc. ''who advised us on cultivation of Pseudomonas''<br />
** Gisela Beissel, Annette Schreer and Kalle Hüser ''for their great patience with our entropy generation''<br />
* '''Forschungszentrum Jülich'''<br />
** Marianne Heß ''who was and is essential for travel organization''<br />
** Dr. Hanno Scharr ''for advice on image analysis''<br />
* '''Fab Lab Aachen'''<br />
** René Bohne, Jan Zimmermann and Jan Thar ''who generously gave us access to the lasercutter and 3D printer''<br />
* '''Others'''<br />
** Dr. Helen Rosenkranz, ABBt, ''for helping us with hundreds of administrative paperwork''<br />
** Dr. Heinz-Albert Becker, NEAnderLab, ''for his help with the organization of the school project''<br />
** Ulrike Eisel, Gymnasium am Neandertal, ''for her help with the organization of the school project''<br />
** Fachschaft Biowissenschaften ''for food supplies and access to their rooms''<br />
** Dr. Joachim Fröhlingsdorf, MakeLight, ''who inspired us to expand our OD/F project''<br />
** Univ.-Prof. Dr. Wolfgang Dott, Uniklinik RWTH Aachen, ''for helpful feedback regarding fields of application for our biosensor''<br />
<br />
Finally, we want to thank '''Simon&nbsp;Unthan''', '''Michael&nbsp;Limberg''', '''Henrik&nbsp;Cordes''', '''Sven&nbsp;Jager''' and '''Team&nbsp;Bielefeld''' for their advice guiding us through our first iGEM participation! Special thanks to Dr.-Ing. Suresh Sudarsan for final checking our wiki for typos!<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/AttributionsTeam:Aachen/Attributions2014-10-18T03:00:08Z<p>Mosthege: /* Attribution of Scientific and General Support */</p>
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<div>__NOTOC__<br />
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# General Support<br />
# Fundraising help and advice<br />
# Partners<br />
# Project support and advice<br />
# Lab support<br />
# Difficult technique support<br />
# Project advisor support<br />
# Wiki support<br />
# Presentation coaching<br />
# Policy & Practices support<br />
# Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.<br />
--><br />
=Members=<br />
<span class="anchor" id="members"></span><br />
<br />
A core concept of iGEM is the collaboration within an interdisciplinary student team. Accordingly, students of different age, gender and field of study came together to found our team in Aachen, in order to realize our project with joint forces and enthusiasm for the overall goal. Working closely together, we therefore not only have the chance to learn from each other, but also to excel ourselves. Using our collective creativity and common responsibility, we will create something special in order to make our contribution to synthetic biology.<br />
<br />
<center><br />
<html><ul class="team-grid" style="width:1040px"><br />
<!-- team member profiles --><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Mosthege" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Michael Osthege </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Man for Everything<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/35/Aachen_team_member_Michael_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Fgohr" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Florian Gohr </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
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King Gibson<br />
<br/><br/><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"> </div></a><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:AZimmermann" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Arne Zimmermann </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
The Patience itself<br />
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<!-- click for more information --><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Nbailly" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Nina Bailly </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Shake Flask Prep Princess<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:VeraA" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Vera Alexandrova </b><br />
<br/><br/><br />
<i> Biology </i><br />
<br/><br/><br />
Goddess of Plasmid Prep<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Pdemling" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Philipp Demling </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Prince of bad Puns<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:R.hanke" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> René Hanke </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Creative Mind and Cook<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:PatrickO" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Patrick Opdenstein </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Graphics God<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/1/1a/Aachen_team_member_Patrick_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Bpeeters" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Björn Peeters </b><br />
<br/><br/><br />
<i> Computational Engineering Science </i><br />
<br/><br/><br />
Hardware Master<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/8d/Aachen_team_member_Bjoern_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Eshani.sood" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Eshani Sood </b><br />
<br/><br/><br />
<i> Biomedical Engineering </i><br />
<br/><br/><br />
Sunshine<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/52/Aachen_team_member_Eshani_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Mjoppich" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Markus Joppich </b><br />
<br/><br/><br />
<i> Computer Science </i><br />
<br/><br/><br />
Software Expert and Travel Assistant<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/83/Aachen_team_member_Markus_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Ansgar" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Ansgar Niemöller </b><br />
<br/><br/><br />
<i> Computational Engineering Science </i><br />
<br/><br/><br />
GUI Master<br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/4e/Aachen_team_member_Ansgar_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
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<a href="https://2014.igem.org/User:J.plum" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Julia Plum </b><br />
<br/><br/><br />
<i> Biology and Business Administration </i><br />
<br/><br/><br />
Fundraising and Balancing<br />
<br/><br/><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c9/Aachen_team_member_Julia_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<b> Stefan Reinhold </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Treasurer<br />
<br/><br/><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0c/Aachen_team_member_Stefan_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<a href="https://2014.igem.org/User:Aschechtel" style="color:black"><br />
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<b> Anna Schechtel </b><br />
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<i> Biology </i><br />
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Chip Queen<br />
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<br />
= Advisors =<br />
<br />
<html><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/5/57/Aachen_team_member_Suresh_01.jpg" width="200px" /></html><br />
<br />
'''Dr.-Ing. Suresh Sudarsan'''<br />
<br />
I'm working as a post-doc with Prof. Blank at RWTH Aachen University. In my Ph.D. I worked under the guidance of Prof. Andreas Schmid (2008-2012) at TU Dortmund, and Prof. Matthias Reuss (2009-2011) at the University of Stuttgart, with a focus on elucidating the link between the central and aromatic metabolism of ''P. putida'' using a systems biology approach.<br />
<br />
I'm fascinated about understanding the collective behavior of microorganisms and their metabolic potential in different niches. In my research, I use tools in ''metabolic engineering & systems biology'' such as metabolomics, fast sampling, kinetic/dynamic modeling and metabolic flux analysis. <br />
<br />
In iGEM, I enjoy working with a group of young scientists from Aachen to achieve our common goal of detecting ''Pseudomonas aeruginosa'' on hard surfaces. Besides science, I try to get myself involved in adventurous sports...but honestly... I just like to relax my day with a delicious meal and a good sci-fi movie!<br />
<br />
<br />
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<br />
'''Dr. rer. nat. Ljubica Vojcic'''<br />
<br />
I am working as a Subgroup Leader in Prof. Schwaneberg research group at the Institute of Biotechnology, RWTH Aachen. The research area of the Schwaneberg group focuses on directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequences of mutational biases on the protein level and developing novel high-throughput screening systems that will lead to improved biocatalysts for prominent applications in industry. In particular, my core expertise is development of high throughput screening systems for different enzyme classes in order to redefine the screening step no longer as bottleneck in directed evolution. <br />
<br />
In iGEM, I enjoyed very much to work with highly motivated and ambitious young scientists from RWTH Aachen University. I truly believe that our collaboration has just started and that we will enjoy jointly solving the scientific challenges in the near future. <br />
<br />
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<br />
=Instructors=<br />
<span class="anchor" id="instructors"></span><br />
== Prof. Dr.-Ing. Lars M. Blank ==<br />
==== RWTH Institute for Applied Microbiology (iAMB) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/4/42/Aachen_LarsBlank.jpg" width="150px" /></html> <br />
Prof. Blank focuses his research on fundamental and applied aspects of microbial metabolism. Of specific interest is the interaction between the metabolic network and the introduced genetic and environmental perturbations. The research on in silico/in vivo metabolic network operations is aimed at a deeper understanding of cell function, with the ultimate goal of rational cell engineering.<br />
<br />
In his teaching, Prof. Blank focuses on the integration of biological concepts with the tools from bioinformatics and engineering. He believes that a sound knowledge base in life sciences is the key for creative and thus successful work in the areas of Metabolic Engineering and Synthetic Biology. Read more about Prof. Blank's work on the [http://www.iamb.rwth-aachen.de/html/members.php?s=det&id=3 iAMB's website].<br />
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<br />
== Prof. Dr. Wolfgang Wiechert ==<br />
==== Forschungszentrum Jülich, Institute of Bio- and Geosciences (IGB-1) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/8/88/Aachen_WolfgangWiechert.jpg" width="150px" /></html> <br />
Prof. Wiechert's main area of work lies within the field of applied systems bio(techno)logy of microorganisms with a special focus on methodological developments for quantitative biology. Characteristics of his research work are a close integration of experimental and theoretical work within multi disciplinary projects. As the head of the Systems Biotechnology research division at Forschungszentrum Jülich, he is developing methods for quantitative metabolomics, fluxomics and proteomics including model based mathematical methods for experimental design, parameter estimation, and process optimization in biotechnological systems. Future work will also incorporate micro fluidic methods for single cell analysis. In general, all research results are used to drive forward the process of gaining knowledge in the course of an iterative improvement of industrial production systems. This proceeds in close cooperation of all working groups at the IBG-1. Together with industrial partners also diverse examples from industry are investigated and further developed. Read more about Prof. Wiechert's work on the [http://www.fz-juelich.de/SharedDocs/Personen/IBG/IBG-1/EN/Research_groups/general/wiechert.html/ IBG-1 website].<br />
<html><br><br><br></p></html><br />
<br />
== Prof. Dr. Ulrich Schwaneberg ==<br />
==== RWTH Institute for Biotechnology, Leibniz Institute for Interactive Materials (DWI) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/f/fe/Aachen_UlrichSchwaneberg.jpg" width="150px" /></html> <br />
The Schwaneberg Group seeks to be at the research frontier in the interdisciplinary field of directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequence of mutational biases on the protein level and developing novel high-throughput screening systems that will ultimately lead to tailored-biocatalysts for significant applications in industry. They train students in the cutting edge technologies of laboratory evolution, biocatalyst engineering and high throughput screening methodologies. The Schwaneberg Group believes in integrating fundamental principles of protein design with environmental awareness in their research and seeks to promote international scientific collaborations. Read more about Prof. Schwaneberg and his work on the [http://www.biotec.rwth-aachen.de/ Schwaneberg Group's website].<br />
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<br />
This project would not have been possible without the generous help and advice from many great people and organizations. We thank all the partners listed below for their essential contributions and financial support that covered registration fees, travel costs, the organization of the meetup in September, laboratory materials and expenses for services and materials.<br />
<br />
=Partners=<br />
<span class="anchor" id="partners"></span><br />
<div align="center"><br />
{|cellpadding="25"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|159px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|318px|link=http://www.niersverband.de/|Niersverband]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Genscript_Logo.png|240px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|193px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|256px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="35"<br />
|[[File:Aachen_bmbf.jpg|326px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|217px|link=http://www.idt-biologika.de/|IDT]]<br />
|}<br />
<br />
{|cellpadding="25"<br />
|[[File:M2p_labs_logo.jpg|103px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|109px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|121px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="25"<br />
|[[File:Aachen_Logo_iAMB.png|229px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|229px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|246px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|}<br />
{|cellpadding="35"<br />
|[[File:Aachen_Logo_ABBt.png|209px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|239px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|209px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|150px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
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<br />
=Attribution of Scientific and General Support=<br />
<span class="anchor" id="support"></span><br />
Great projects do not only depend on solid funding, but even more on the invaluable support by great people. We found great people not only in our 'home institute' the iAMB, but across many partner institutions.<br />
<br />
<br />
* '''Institute for Biology VII''' (Molecular Biotechnology)<br />
** Dr. Ulrich Commandeur ''for giving us access to essential resources of the bio7''<br />
** Christina Dickmeis M.Sc. ''who answered lots of questions''<br />
* '''Institute for Biotechnology'''<br />
** David Schönauer and Alan Mertens M.Sc. ''who helped us to purify proteins''<br />
* '''Helmholtz Institute for Biomedical Engineering'''<br />
** Prof. Dr. Lothar Elling and Sophia Böcker, M.Sc. ''for giving us access to their gal-3 expression plasmid''<br />
* '''Institute for Applied Microbiology''' (iAMB)<br />
** Prof. Dr. Miriam Agler-Rosenbaum ''for scientific information on quorum sensing''<br />
** Dr. Martin Zimmermann ''who counseled us on balancing iGEM with our studies''<br />
** Dr. Andrea Germer ''for teaching us Gibson cloning''<br />
** Erick Bosire Maosa M.Sc. ''who advised us on cultivation of Pseudomonas''<br />
** Gisela Beissel, Annette Schreer and Kalle Hüser ''for their great patience with our entropy generation''<br />
* '''Forschungszentrum Jülich'''<br />
** Marianne Heß ''who was and is essential for travel organization''<br />
** Dr. Hanno Scharr ''for advice on image analysis''<br />
* '''Fab Lab Aachen'''<br />
** René Bohne, Jan Zimmermann and Jan Thar ''who generously gave us access to the lasercutter and 3D printer''<br />
* '''Others'''<br />
** Dr. Helen Rosenkranz, ABBt, ''for helping us with hundreds of administrative paperwork''<br />
** Dr. Heinz-Albert Becker, NEAnderLab, ''for his help with the organization of the school project''<br />
** Ulrike Eisel, Gymnasium am Neandertal, ''for her help with the organization of the school project''<br />
** Fachschaft Biowissenschaften ''for food supplies and access to their rooms''<br />
** Dr. Joachim Fröhlingsdorf, MakeLight, ''who inspired us to expand our OD/F project''<br />
** Univ.-Prof. Dr. Wolfgang Dott, Uniklinik RWTH Aachen, ''for helpful feedback regarding fields of application for our biosensor''<br />
<br />
Finally, we want to thank Simon&nbsp;Unthan, Michael&nbsp;Limberg, Henrik&nbsp;Cordes, Sven&nbsp;Jager and Team&nbsp;Bielefeld for their advice guiding us through our first iGEM participation! Special thanks to Dr.-Ing. Suresh Sudarsan for final checking our wiki for typos!<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/AttributionsTeam:Aachen/Attributions2014-10-18T02:59:08Z<p>Mosthege: /* Scientific and General Support */</p>
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# General Support<br />
# Fundraising help and advice<br />
# Partners<br />
# Project support and advice<br />
# Lab support<br />
# Difficult technique support<br />
# Project advisor support<br />
# Wiki support<br />
# Presentation coaching<br />
# Policy & Practices support<br />
# Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.<br />
--><br />
=Members=<br />
<span class="anchor" id="members"></span><br />
<br />
A core concept of iGEM is the collaboration within an interdisciplinary student team. Accordingly, students of different age, gender and field of study came together to found our team in Aachen, in order to realize our project with joint forces and enthusiasm for the overall goal. Working closely together, we therefore not only have the chance to learn from each other, but also to excel ourselves. Using our collective creativity and common responsibility, we will create something special in order to make our contribution to synthetic biology.<br />
<br />
<center><br />
<html><ul class="team-grid" style="width:1040px"><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Mosthege" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Michael Osthege </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Man for Everything<br />
<br/><br/><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/35/Aachen_team_member_Michael_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<a href="https://2014.igem.org/User:Fgohr" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
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<b> Florian Gohr </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
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King Gibson<br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%;height: 180px; width: 180px;"> </div></a><br />
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<a href="https://2014.igem.org/User:AZimmermann" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
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<b> Arne Zimmermann </b><br />
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<i> Molecular and Applied Biotechnology </i><br />
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The Patience itself<br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Nbailly" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
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<b> Nina Bailly </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
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Shake Flask Prep Princess<br />
<br/><br/><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:VeraA" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Vera Alexandrova </b><br />
<br/><br/><br />
<i> Biology </i><br />
<br/><br/><br />
Goddess of Plasmid Prep<br />
<br/><br/><br />
<!-- click for more information --><br />
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<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Pdemling" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Philipp Demling </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Prince of bad Puns<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:R.hanke" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> René Hanke </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Creative Mind and Cook<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:PatrickO" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Patrick Opdenstein </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Graphics God<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/1/1a/Aachen_team_member_Patrick_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Bpeeters" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Björn Peeters </b><br />
<br/><br/><br />
<i> Computational Engineering Science </i><br />
<br/><br/><br />
Hardware Master<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/8d/Aachen_team_member_Bjoern_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Eshani.sood" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Eshani Sood </b><br />
<br/><br/><br />
<i> Biomedical Engineering </i><br />
<br/><br/><br />
Sunshine<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/52/Aachen_team_member_Eshani_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Mjoppich" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Markus Joppich </b><br />
<br/><br/><br />
<i> Computer Science </i><br />
<br/><br/><br />
Software Expert and Travel Assistant<br />
<br/><br/><br />
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</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/83/Aachen_team_member_Markus_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
<br />
<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Ansgar" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Ansgar Niemöller </b><br />
<br/><br/><br />
<i> Computational Engineering Science </i><br />
<br/><br/><br />
GUI Master<br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/4e/Aachen_team_member_Ansgar_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:J.plum" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Julia Plum </b><br />
<br/><br/><br />
<i> Biology and Business Administration </i><br />
<br/><br/><br />
Fundraising and Balancing<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c9/Aachen_team_member_Julia_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:StefanReinhold" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Stefan Reinhold </b><br />
<br/><br/><br />
<i> Molecular and Applied Biotechnology </i><br />
<br/><br/><br />
Treasurer<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0c/Aachen_team_member_Stefan_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
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<li style="width:186px;margin-left: 11px;margin-right: 11px;margin-bottom: 11px;margin-top: 11px;"><br />
<a href="https://2014.igem.org/User:Aschechtel" style="color:black"><br />
<div class="team-item team-info" style="height: 180px; width: 180px;"><br />
<br/><br/><br />
<b> Anna Schechtel </b><br />
<br/><br/><br />
<i> Biology </i><br />
<br/><br/><br />
Chip Queen<br />
<br/><br/><br />
<!-- click for more information --><br />
</div><br />
<div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/a/a4/Aachen_team_member_Anna_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%; height: 180px; width: 180px;"> </div></a><br />
</li><br />
</ul><br />
</html><br />
</center><br />
<br />
= Advisors =<br />
<br />
<html><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/5/57/Aachen_team_member_Suresh_01.jpg" width="200px" /></html><br />
<br />
'''Dr.-Ing. Suresh Sudarsan'''<br />
<br />
I'm working as a post-doc with Prof. Blank at RWTH Aachen University. In my Ph.D. I worked under the guidance of Prof. Andreas Schmid (2008-2012) at TU Dortmund, and Prof. Matthias Reuss (2009-2011) at the University of Stuttgart, with a focus on elucidating the link between the central and aromatic metabolism of ''P. putida'' using a systems biology approach.<br />
<br />
I'm fascinated about understanding the collective behavior of microorganisms and their metabolic potential in different niches. In my research, I use tools in ''metabolic engineering & systems biology'' such as metabolomics, fast sampling, kinetic/dynamic modeling and metabolic flux analysis. <br />
<br />
In iGEM, I enjoy working with a group of young scientists from Aachen to achieve our common goal of detecting ''Pseudomonas aeruginosa'' on hard surfaces. Besides science, I try to get myself involved in adventurous sports...but honestly... I just like to relax my day with a delicious meal and a good sci-fi movie!<br />
<br />
<br />
<br />
<html><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/c/c8/Aachen_team_member_Ljubica.jpg" width="200px" /></html><br />
<br />
'''Dr. rer. nat. Ljubica Vojcic'''<br />
<br />
I am working as a Subgroup Leader in Prof. Schwaneberg research group at the Institute of Biotechnology, RWTH Aachen. The research area of the Schwaneberg group focuses on directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequences of mutational biases on the protein level and developing novel high-throughput screening systems that will lead to improved biocatalysts for prominent applications in industry. In particular, my core expertise is development of high throughput screening systems for different enzyme classes in order to redefine the screening step no longer as bottleneck in directed evolution. <br />
<br />
In iGEM, I enjoyed very much to work with highly motivated and ambitious young scientists from RWTH Aachen University. I truly believe that our collaboration has just started and that we will enjoy jointly solving the scientific challenges in the near future. <br />
<br />
<br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
=Instructors=<br />
<span class="anchor" id="instructors"></span><br />
== Prof. Dr.-Ing. Lars M. Blank ==<br />
==== RWTH Institute for Applied Microbiology (iAMB) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/4/42/Aachen_LarsBlank.jpg" width="150px" /></html> <br />
Prof. Blank focuses his research on fundamental and applied aspects of microbial metabolism. Of specific interest is the interaction between the metabolic network and the introduced genetic and environmental perturbations. The research on in silico/in vivo metabolic network operations is aimed at a deeper understanding of cell function, with the ultimate goal of rational cell engineering.<br />
<br />
In his teaching, Prof. Blank focuses on the integration of biological concepts with the tools from bioinformatics and engineering. He believes that a sound knowledge base in life sciences is the key for creative and thus successful work in the areas of Metabolic Engineering and Synthetic Biology. Read more about Prof. Blank's work on the [http://www.iamb.rwth-aachen.de/html/members.php?s=det&id=3 iAMB's website].<br />
<html><br><br><br></p></html><br />
<br />
== Prof. Dr. Wolfgang Wiechert ==<br />
==== Forschungszentrum Jülich, Institute of Bio- and Geosciences (IGB-1) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/8/88/Aachen_WolfgangWiechert.jpg" width="150px" /></html> <br />
Prof. Wiechert's main area of work lies within the field of applied systems bio(techno)logy of microorganisms with a special focus on methodological developments for quantitative biology. Characteristics of his research work are a close integration of experimental and theoretical work within multi disciplinary projects. As the head of the Systems Biotechnology research division at Forschungszentrum Jülich, he is developing methods for quantitative metabolomics, fluxomics and proteomics including model based mathematical methods for experimental design, parameter estimation, and process optimization in biotechnological systems. Future work will also incorporate micro fluidic methods for single cell analysis. In general, all research results are used to drive forward the process of gaining knowledge in the course of an iterative improvement of industrial production systems. This proceeds in close cooperation of all working groups at the IBG-1. Together with industrial partners also diverse examples from industry are investigated and further developed. Read more about Prof. Wiechert's work on the [http://www.fz-juelich.de/SharedDocs/Personen/IBG/IBG-1/EN/Research_groups/general/wiechert.html/ IBG-1 website].<br />
<html><br><br><br></p></html><br />
<br />
== Prof. Dr. Ulrich Schwaneberg ==<br />
==== RWTH Institute for Biotechnology, Leibniz Institute for Interactive Materials (DWI) ====<br />
<html><p><img class="imgshadow" style="float: left;" src="https://static.igem.org/mediawiki/2014/f/fe/Aachen_UlrichSchwaneberg.jpg" width="150px" /></html> <br />
The Schwaneberg Group seeks to be at the research frontier in the interdisciplinary field of directed protein evolution by developing novel methods for generating diversity at the gene level, analyzing consequence of mutational biases on the protein level and developing novel high-throughput screening systems that will ultimately lead to tailored-biocatalysts for significant applications in industry. They train students in the cutting edge technologies of laboratory evolution, biocatalyst engineering and high throughput screening methodologies. The Schwaneberg Group believes in integrating fundamental principles of protein design with environmental awareness in their research and seeks to promote international scientific collaborations. Read more about Prof. Schwaneberg and his work on the [http://www.biotec.rwth-aachen.de/ Schwaneberg Group's website].<br />
<html><br><br><br></p><span id="team"></span></html><br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
This project would not have been possible without the generous help and advice from many great people and organizations. We thank all the partners listed below for their essential contributions and financial support that covered registration fees, travel costs, the organization of the meetup in September, laboratory materials and expenses for services and materials.<br />
<br />
=Partners=<br />
<span class="anchor" id="partners"></span><br />
<div align="center"><br />
{|cellpadding="25"<br />
|[[File:Logo_Buergerstiftung-Aachen_hoch.jpg|159px|center|link=http://www.buergerstiftung-aachen.de|Bürgerstiftung Aachen]]<br />
|[[File:Aachen_Niersverband_Firmenlogo.jpg|318px|link=http://www.niersverband.de/|Niersverband]]<br />
|}<br />
{|cellpadding="13"<br />
|[[File:Aachen_Genscript_Logo.png|240px|link=http://www.genscript.com/|Genscript]]<br />
|[[File:Aachen_Eurofinsgenomics.png|193px|link=http://www.eurofinsgenomics.eu/|Eurofins Genomics]]<br />
|[[File:Aachen_Labomedic_Logo.jpg|256px|link=http://www.labomedic.de/|Labomedic]]<br />
|}<br />
<br />
{|cellpadding="35"<br />
|[[File:Aachen_bmbf.jpg|326px|link=http://www.bmbf.de/de/24140.php|BMBF]]<br />
|[[File:Aachen_idt_.png|217px|link=http://www.idt-biologika.de/|IDT]]<br />
|}<br />
<br />
{|cellpadding="25"<br />
|[[File:M2p_labs_logo.jpg|103px|link=http://www.m2p-labs.com/|m2p labs]]<br />
|[[File:Aachen_Roth_Logo.png|109px|link=http://www.carlroth.com/pages/index/COM/index.jsp?market=COM&lang=en-com|Carl Roth]]<br />
|[[File:Aachen_ProRWTH_logo.png|121px|link=http://www.prorwth.de/|pro RWTH]]<br />
|}<br />
{|cellpadding="25"<br />
|[[File:Aachen_Logo_iAMB.png|229px|link=http://www.iamb.rwth-aachen.de|Institute of Applied Microbiology - iAMB]]<br />
|[[File:Aachen_Logo_bio7.png|229px|link=http://www.molbiotech.rwth-aachen.de|Institute for Molecular Biotechnology]]<br />
|[[File:Aachen_Logo_HISynBio.png|246px|link=http://www.helmholtz.de/en/about_us/initiating_and_networking/assuring_excellence/synthetic_biology|Helmholtz Association - Initiative on Synthetic Biology]]<br />
|}<br />
{|cellpadding="35"<br />
|[[File:Aachen_Logo_ABBt.png|209px|link=http://www.biologie.rwth-aachen.de|Aachen Biology and Biotechnology - ABBt]]<br />
|[[File:Aachen_juelich.png|239px|link=http://www.fz-juelich.de/|Forschungszentrum Jülich]]<br />
|[[File:Aachen_FabLabAachenLogo.jpg|209px|link=http://hci.rwth-aachen.de/fablab|Fab Lab Aachen]]<br />
|[[File:Aachen_Schwaneberg_Group.png|150px|link=http://www.biotec.rwth-aachen.de|Schwaneberg Group]]<br />
|}<br />
<div><br />
<br />
{{Team:Aachen/BlockSeparator}}<br />
<br />
=Attribution of Scientific and General Support=<br />
<span class="anchor" id="support"></span><br />
Great projects do not only depend on solid funding, but even more on the invaluable support by great people. We found great people not only in our 'home institute' the iAMB, but across many partner institutions.<br />
<br />
<br />
* '''Institute for Biology VII''' (Molecular Biotechnology)<br />
** Dr. Ulrich Commandeur ''for giving us access to essential resources of the bio7''<br />
** Christina Dickmeis M.Sc. ''who answered lots of questions''<br />
* '''Institute for Biotechnology'''<br />
** David Schönauer and Alan Mertens M.Sc. ''who helped us to purify proteins''<br />
* '''Helmholtz Institute for Biomedical Engineering'''<br />
** Prof. Dr. Lothar Elling and Sophia Böcker, M.Sc. ''for giving us access to their gal-3 expression plasmid''<br />
* '''Institute for Applied Microbiology''' (iAMB)<br />
** Prof. Dr. Miriam Agler-Rosenbaum ''for scientific information on quorum sensing''<br />
** Dr. Martin Zimmermann ''who counseled us on balancing iGEM with our studies''<br />
** Dr. Andrea Germer ''for teaching us Gibson cloning''<br />
** Erick Bosire Maosa M.Sc. ''who advised us on cultivation of Pseudomonas''<br />
** Gisela Beissel, Annette Schreer and Kalle Hüser ''for their great patience with our entropy generation''<br />
* '''Forschungszentrum Jülich'''<br />
** Marianne Heß ''who was and is essential for travel organization''<br />
** Dr. Hanno Scharr ''for advice on image analysis''<br />
* '''Fab Lab Aachen'''<br />
** René Bohne, Jan Zimmermann and Jan Thar ''who generously gave us access to the lasercutter and 3D printer''<br />
* '''Others'''<br />
** Dr. Helen Rosenkranz, ABBt, ''for helping us with hundreds of administrative paperwork''<br />
** Dr. Heinz-Albert Becker, NEAnderLab, ''for his help with the organization of the school project''<br />
** Ulrike Eisel, Gymnasium am Neandertal, ''for her help with the organization of the school project''<br />
** Fachschaft Biowissenschaften ''for food supplies and access to their rooms''<br />
** Dr. Joachim Fröhlingsdorf, MakeLight, ''who inspired us to expand our OD/F project''<br />
** Univ.-Prof. Dr. Wolfgang Dott ''for helpful feedback regarding fields of application for our biosensor''<br />
<br />
Finally, we want to thank Simon&nbsp;Unthan, Michael&nbsp;Limberg, Henrik&nbsp;Cordes, Sven&nbsp;Jager and Team&nbsp;Bielefeld for their advice guiding us through our first iGEM participation! Special thanks to Dr.-Ing. Suresh Sudarsan for final checking our wiki for typos!<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Collaborations/BraunschweigTeam:Aachen/Collaborations/Braunschweig2014-10-18T02:42:25Z<p>Mosthege: /* A low-cost DIY methane sensor for Team Braunschweig */</p>
<hr />
<div>__NOTOC__<br />
{{Team:Aachen/Header}}<br />
<br />
= A low-cost DIY methane sensor for [[Team:Braunschweig/Team-content#Collaborations|Team Braunschweig]] =<br />
Our colleagues from Braunschweig are cultivating microorganisms that grow on methane (CH{{sub|4}}) which is supplied via the gas phase. To monitor the methane uptake by their ''E. co(w)li'', they would like to measure the methane concentration in the gas phase. With the standard analytical methods in the lab, this is very difficult, but when they told us about their problem, we knew that we might be able to help them. We assembled an Arduino-based methane sensor and wrote a program to record the data.<br />
<br />
The componentes for the methane sensor include:<br />
* [http://www.dx.com/p/arduino-nano-v3-0-81877 Arduino Nano V3 clone] [[File:Aachen_ArduinoNano.jpg|90px]]<br />
* [http://www.sainsmart.com/sainsmart-mq-4-methane-gas-sensor-natural-coal-co-detector-module-for-arduino.html SainSmart MQ-4 Gas Sensor] [[File:Aachen_SainSmartMQ4.jpg|90px]]<br />
* A [http://www.dx.com/p/syb-170-mini-breadboard-for-diy-project-red-140101#.U-ng9VLwC7Q Breadboard] and some wires [[File:Aachen_Breadboard.jpg|90px]]<br />
<br />
To set up the electronics for a measurement, the sensor and the Arduino have to be connected according to the following scheme:<br />
<br />
<center><br />
{{Team:Aachen/Figure|Aachen_Collaboration-BS-Schematic.png|align=center|title=Connecting the MQ-4 module to the Arduino|subtitle=Only three wires are required to connect the MQ-4 module to the Arduino Nano.|width=700px}}<br />
</center><br />
<br />
<br />
We sent all the components to Braunschweig and they assembled the Arduino and sensor module in an incubation chamber:<br />
<br />
{{Team:Aachen/FigureDual|TU-BS_sensor_sensor-in-pot.jpg|TU-BS_sensor_measuringsystem.jpg|title1=MQ-4 sensor in the gas phase|title2=Online data on a laptop|subtitle1=For anaerobic cultivation, a wireless connection might be better.|subtitle2=The Arduino is connected to a laptop running our Serial&nbsp;Client software to plot and collect online data.|width=450px}}<br />
<br />
The software used to monitor the online methane concentration data is the [[Team:Aachen/Notebook/Software/Serial_Client|Serial Client]] that was also developed by our team.<br />
<br />
<br />
{{Team:Aachen/Footer}}</div>Mosthegehttp://2014.igem.org/Team:Aachen/Notebook/LabEquipmentTeam:Aachen/Notebook/LabEquipment2014-10-18T02:37:30Z<p>Mosthege: </p>
<hr />
<div>{{Team:Aachen/Header}}<br />
<br />
= Lab Equipment =<br />
<br />
<center><br />
Equipment used in our project:<br />
{| class="wikitable sortable"<br />
! align="center" |'''Equipment'''<br />
!! align="center" |'''Company'''<br />
|-<br />
| Avanti 30 Centrifuge || Beckman<br />
|-<br />
| Äkta Prime Plus || GE Healthcare<br />
|-<br />
| Biofuge "pico" Heraues || Thermo Scientific<br />
|-<br />
| Cleanbench || Scanlaf<br />
|-<br />
| Clark Electrode || Hamilton<br />
|-<br />
| GenePulser XCell || BioRad <br />
|-<br />
| Fermenter 1L || Sartorius<br />
|-<br />
| Fermenter control unit Biostat A-Plus || Sartorius<br />
|-<br />
| Freezer || Thermo Scientific <br />
|-<br />
| Geldoc || Herolab<br />
|-<br />
| Geldoc XR+ || Biorad<br />
|-<br />
| Heatplate || Yellow line <br />
|-<br />
| Megafuge 16R Heraeus || Thermo Scientific <br />
|-<br />
| Incubator || VWR<br />
|-<br />
| Mastercycler gradient || Eppendorf<br />
|-<br />
| Micro Centrifuge || Roth<br />
|-<br />
| Microscope ICG-50|| Leica<br />
|-<br />
| Microscope AF6000 LX Videomicroscope || Leica<br />
|-<br />
| Microscope Software LAS AF 3.2.09652 || Leica<br />
|-<br />
| Microscope Orthoplan || Leica<br />
|-<br />
| NanoVue Plus || GE Healthcare<br />
|-<br />
| Pico 17 Centrifuge || Thermo Scientific<br />
|-<br />
| PowerPac 200 + 300 || BioRad<br />
|-<br />
| pH Electrode || Mettler Toledo <br />
|-<br />
| pH/ORP Meter || Hanna Instruments<br />
|-<br />
| Programmable Thermal Controller || MJ Research<br />
|-<br />
| Pipet boy || Sartorius <br />
|-<br />
| Stirrer || Unkermotoren germany<br />
|-<br />
| Shaker || New Brunswick<br />
|-<br />
| Spectrophotometer 1200 || Fisher Bioblock Scientific<br />
|-<br />
| Platereader Synergy MX || BioTek<br />
|-<br />
| Platereader Software GenX || BioTek<br />
|-<br />
| Thermomixer Compact || Eppendorf<br />
|-<br />
| Thermocylcler Primus 25|| MWG Biotech<br />
|-<br />
| Waterbath || Lauda<br />
|-<br />
| Waterbath Frigomix 1000 || Sartorius<br />
|-<br />
| Varifuge 3.0R || Heraues <br />
|-<br />
| Vortexer || Fisher Scientific<br />
|-<br />
<br />
<br />
|}<br />
<br />
<br />
Kits:<br />
{| class="wikitable sortable"<br />
! align="center" |'''Kit'''<br />
!! align="center" |'''Company'''<br />
|-<br />
| GoTaq Green Mastermix || Promega <br />
|-<br />
| Gibson assembly cloning kit || NEB <br />
|-<br />
| Illustra Plasmid Prep Mini Spin Kit || GE Healthcare <br />
|-<br />
| PCR DNA and Gel Band Purification Kit || GE Healthcare <br />
|-<br />
| iGEM Biobrick Assembly Kit || NEB <br />
|-<br />
| Transformation efficiency kit || iGEM Headquarters<br />
|-<br />
| KAPA2G Fast ReadyMix || Kapa Biosystems<br />
|-<br />
|}<br />
</center><br />
{{Team:Aachen/Footer}}</div>Mosthege