http://2014.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=Kenlaukit&year=&month=2014.igem.org - User contributions [en]2024-03-29T10:15:36ZFrom 2014.igem.orgMediaWiki 1.16.5http://2014.igem.org/File:CityU_HK_fad_Gas_chromatography4.jpgFile:CityU HK fad Gas chromatography4.jpg2014-10-17T15:11:32Z<p>Kenlaukit: </p>
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<div></div>Kenlaukithttp://2014.igem.org/File:CityU_HK_fad_Gas_chromatography3.jpgFile:CityU HK fad Gas chromatography3.jpg2014-10-17T15:11:23Z<p>Kenlaukit: </p>
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<div></div>Kenlaukithttp://2014.igem.org/File:CityU_HK_fad_Gas_chromatography2.jpgFile:CityU HK fad Gas chromatography2.jpg2014-10-17T15:11:14Z<p>Kenlaukit: </p>
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<div></div>Kenlaukithttp://2014.igem.org/File:CityU_HK_fad_Gas_chromatography1.jpgFile:CityU HK fad Gas chromatography1.jpg2014-10-17T15:11:05Z<p>Kenlaukit: </p>
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<div></div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/overviewTeam:CityU HK/project/overview2014-10-17T14:56:35Z<p>Kenlaukit: </p>
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<p class="content">Obesity is a worldwide problem that contributes to a variety of human diseases such as diabetes and cancer, as well as psychological problems such as eating disorder and anxiety. Traditional remedies such as dieting or over-exercising can be ineffective and often lead to depression, given that both means could lead to obvious physical and mental unpleasantness. Obesity, in many cases, is linked to the accumulation of excessive free fatty acids from food. Therefore removing these fatty acids from the system could be the key to combating obesity. In an attempt to alleviating the obesity problem without causing adverse impact on one’s quality of life, we genetically engineered an <i>Escherichia coli</i> strain to confer upon it the capacity to both remove excess fat from our diet and convert it into a useful metabolic intermediate, &#945; linolenic acid (ALA). We named this genetically modified bacterium, “Fit Coli”.<br><br><br />
<br />
We designed Fit Coli to do the job in several steps. The first step involves increasing its ability to take up excess free fatty acids from the external environment (e.g. human gut) which, theoretically, can be achieved by overexpressing the <i>fadL</i> and <i>fadD</i> genes in <i>E. coli</i>. <i>fadL</i> codes for a fatty acid transporter protein that moves free fatty acids across the outer cell membrane into the periplasmic space. <i>fadD</i>, on the other hand, codes for a fatty acyl-CoA synthetase which adds a coenzyme A (CoA) moiety to fatty acids and then transports the resulting fatty acyl-CoA across the inner membrane into the cytosol. <br><br><br />
<br />
Next, <i>E. coli.</i> is engineered to overexpress the <i>tesA</i> gene that codes for an acyl-CoA thioesterase which removes the CoA moiety from fatty acyl-CoA molecules, thereby restoring the molecule back to the form of free fatty acids. This strategy is aimed at diverting fatty acids from the beta-oxidation pathway which degrades fatty acids into acetyl-CoA to produce ATP. Lastly, the free fatty acids are converted into ALA under the catalysis of the three engineered enzymes - ∆9 desaturase, ∆12 desaturase and ∆15 desaturase – in Fit Coli. <br><br><br />
<br />
In short, Fit Coli is designed to combat obesity by its ability (1) to take up various excess C18 fatty acids such as stearic acid, oleic acid and linoleic acid present in our foods and (2) to convert them into ALA which can then be used by the human body to make docosahexaenoic acid/eicosanoids that are beneficial to humans.<br><br> </p><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/module_desaturaseTeam:CityU HK/project/module desaturase2014-10-17T14:50:27Z<p>Kenlaukit: </p>
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<li><a href='https://2014.igem.org/Team:CityU_HK/project/module_fad'><span>FadD & FadL Module</span></a></li><br />
<li><a href='https://2014.igem.org/Team:CityU_HK/project/module_tesA'><span>'TesA Module</span></a></li><br />
<li class='last'><a href='https://2014.igem.org/Team:CityU_HK/project/module_desaturase'><span>Desaturase Module</span></a></li><br />
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<h3> Desaturase Module Description</h3><br><br />
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<p class="content1">After absorption of exogenous fatty acids from the environment, the enzymes, Δ9-, Δ12- and Δ15-desaturases catalyze the conversion of fatty acids (stearic acid) into α-linolenic acid, ALA (Qiu, 2002). Because <i>E. coli</i> originally lacks the genes encoding the desaturases, we have to construct and transform the recombinant DNA plasmid containing them into <i>E. coli.</i>. <br><br><br />
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The gene sequences of the three desaturases can be obtained from Synechocystis, and optimized for expression in <i>E. coli</i>. After transformation of the constructed plasmid, the bacterium is able to produce the corresponding enzymes. Δ9 desaturase removes two hydrogen atoms from carbon 9 and carbon 10 of the fatty acid and a double bond will be formed there. <br><br><br />
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Similarly, Δ12 desaturase works between carbon 12 and carbon 13 while Δ15 desaturase works between carbon 15 and carbon 16. After completion of the reactions, the fatty acid (stearic acid) will be converted into ALA (Figure 1). When ALA is released from <i>E. coli</i> and absorbed by human cells, ALA will be further converted into docosahexaenoic acid, DHA. Since human cells contain genes encoding the remaining desaturases and elongases that are necessary for the conversion of ALA into DHA (Innis, 2007), which is our final target product.</p><br><br />
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<h2 class="figure">Figure 1. Conversion of stearic acid into α-linolenic acid by three desaturases.</h2><br><br />
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<p id="reference"><b>References:</b><br><br><br />
Innis, S. M. (2007). Dietary (n-3) fatty acids and brain development. The Journal of Nutrition. 137: 855.<br><br><br />
Qiu, X. (2003). Biosynthesis of docosahenaenoic acid (DHA, 22:6-4, 7, 10, 13, 16, 19): Two distinct pathways. Prostaglandins, Leukotrienes and Essential Fatty Acids. 68(2003): 181-182.</p><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/overviewTeam:CityU HK/project/overview2014-10-17T14:49:38Z<p>Kenlaukit: </p>
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<p class="content">Obesity is a worldwide problem that contributes to a variety of human diseases such as diabetes and cancer, as well as psychological problems such as eating disorder and anxiety. Traditional remedies such as dieting or over-exercising can be ineffective and often lead to depression, given that both means could lead to obvious physical and mental unpleasantness. Obesity, in many cases, is linked to the accumulation of excessive free fatty acids from food. Therefore removing these fatty acids from the system could be the key to combating obesity. In an attempt to alleviating the obesity problem without causing adverse impact on one’s quality of life, we genetically engineered an <i>Escherichia coli</i> strain to confer upon it the capacity to both remove excess fat from our diet and convert it into a useful metabolic intermediate, &#945; linolenic acid (ALA). We named this genetically modified bacterium, “Fit Coli”.<br><br><br />
<br />
We designed Fit Coli to do the job in several steps. The first step involves increasing its ability to take up excess free fatty acids from the external environment (e.g. human gut) which, theoretically, can be achieved by overexpressing the <i>fadL</i> and <i>fadD</i> genes in <i>E. coli</i>. <i>fadL</i> codes for a fatty acid transporter protein that moves free fatty acids across the outer cell membrane into the periplasmic space. <i>fadD</i>, on the other hand, codes for a fatty acyl-CoA synthetase which adds a coenzyme A (CoA) moiety to fatty acids and then transports the resulting fatty acyl-CoA across the inner membrane into the cytosol. <br><br><br />
<br />
Next, <i>E. coli.</i> is engineered to overexpress the <i>tesA</i> gene that codes for an acyl-CoA thioesterase which removes the CoA moiety from fatty acyl-CoA molecules, thereby restoring the molecule back to the form of free fatty acids. This strategy is aimed at diverting fatty acids from the beta-oxidation pathway which degrades fatty acids into acetyl-CoA to produce ATP. Lastly, the free fatty acids are converted into ALA under the catalysis of the three engineered enzymes - ∆9 desaturase, ∆12 desaturase and ∆15 desaturase – in the Fit Coli. <br><br><br />
<br />
In short, Fit Coli is designed to combat obesity by its ability (1) to take up various excess C18 fatty acids such as stearic acid, oleic acid and linoleic acid present in our foods and (2) to convert them into ALA which can then be used by the human body to make docosahexaenoic acid/eicosanoids that are beneficial to humans.<br><br> </p><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/overviewTeam:CityU HK/project/overview2014-10-17T14:49:32Z<p>Kenlaukit: </p>
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<img class="displayed" src="https://static.igem.org/mediawiki/2014/2/29/CityU_HK_overview.jpg" width="65%"><br />
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<p class="content">Obesity is a worldwide problem that contributes to a variety of human diseases such as diabetes and cancer, as well as psychological problems such as eating disorder and anxiety. Traditional remedies such as dieting or over-exercising can be ineffective and often lead to depression, given that both means could lead to obvious physical and mental unpleasantness. Obesity, in many cases, is linked to the accumulation of excessive free fatty acids from food. Therefore removing these fatty acids from the system could be the key to combating obesity. In an attempt to alleviating the obesity problem without causing adverse impact on one’s quality of life, we genetically engineered an <i>Escherichia coli</i> strain to confer upon it the capacity to both remove excess fat from our diet and convert it into a useful metabolic intermediate, &#945 linolenic acid (ALA). We named this genetically modified bacterium, “Fit Coli”.<br><br><br />
<br />
We designed Fit Coli to do the job in several steps. The first step involves increasing its ability to take up excess free fatty acids from the external environment (e.g. human gut) which, theoretically, can be achieved by overexpressing the <i>fadL</i> and <i>fadD</i> genes in <i>E. coli</i>. <i>fadL</i> codes for a fatty acid transporter protein that moves free fatty acids across the outer cell membrane into the periplasmic space. <i>fadD</i>, on the other hand, codes for a fatty acyl-CoA synthetase which adds a coenzyme A (CoA) moiety to fatty acids and then transports the resulting fatty acyl-CoA across the inner membrane into the cytosol. <br><br><br />
<br />
Next, <i>E. coli.</i> is engineered to overexpress the <i>tesA</i> gene that codes for an acyl-CoA thioesterase which removes the CoA moiety from fatty acyl-CoA molecules, thereby restoring the molecule back to the form of free fatty acids. This strategy is aimed at diverting fatty acids from the beta-oxidation pathway which degrades fatty acids into acetyl-CoA to produce ATP. Lastly, the free fatty acids are converted into ALA under the catalysis of the three engineered enzymes - ∆9 desaturase, ∆12 desaturase and ∆15 desaturase – in the Fit Coli. <br><br><br />
<br />
In short, Fit Coli is designed to combat obesity by its ability (1) to take up various excess C18 fatty acids such as stearic acid, oleic acid and linoleic acid present in our foods and (2) to convert them into ALA which can then be used by the human body to make docosahexaenoic acid/eicosanoids that are beneficial to humans.<br><br> </p><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/overviewTeam:CityU HK/project/overview2014-10-17T14:48:50Z<p>Kenlaukit: </p>
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<br />
<img class="displayed" src="https://static.igem.org/mediawiki/2014/2/29/CityU_HK_overview.jpg" width="65%"><br />
<br />
<p class="content">Obesity is a worldwide problem that contributes to a variety of human diseases such as diabetes and cancer, as well as psychological problems such as eating disorder and anxiety. Traditional remedies such as dieting or over-exercising can be ineffective and often lead to depression, given that both means could lead to obvious physical and mental unpleasantness. Obesity, in many cases, is linked to the accumulation of excessive free fatty acids from food. Therefore removing these fatty acids from the system could be the key to combating obesity. In an attempt to alleviating the obesity problem without causing adverse impact on one’s quality of life, we genetically engineered an <i>Escherichia coli</i> strain to confer upon it the capacity to both remove excess fat from our diet and convert it into a useful metabolic intermediate, a linolenic acid (ALA). We named this genetically modified bacterium, “Fit Coli”.<br><br><br />
<br />
We designed Fit Coli to do the job in several steps. The first step involves increasing its ability to take up excess free fatty acids from the external environment (e.g. human gut) which, theoretically, can be achieved by overexpressing the <i>fadL</i> and <i>fadD</i> genes in <i>E. coli</i>. <i>fadL</i> codes for a fatty acid transporter protein that moves free fatty acids across the outer cell membrane into the periplasmic space. <i>fadD</i>, on the other hand, codes for a fatty acyl-CoA synthetase which adds a coenzyme A (CoA) moiety to fatty acids and then transports the resulting fatty acyl-CoA across the inner membrane into the cytosol. <br><br><br />
<br />
Next, <i>E. coli.</i> is engineered to overexpress the <i>tesA</i> gene that codes for an acyl-CoA thioesterase which removes the CoA moiety from fatty acyl-CoA molecules, thereby restoring the molecule back to the form of free fatty acids. This strategy is aimed at diverting fatty acids from the beta-oxidation pathway which degrades fatty acids into acetyl-CoA to produce ATP. Lastly, the free fatty acids are converted into ALA under the catalysis of the three engineered enzymes - ∆9 desaturase, ∆12 desaturase and ∆15 desaturase – in the Fit Coli. <br><br><br />
<br />
In short, Fit Coli is designed to combat obesity by its ability (1) to take up various excess C18 fatty acids such as stearic acid, oleic acid and linoleic acid present in our foods and (2) to convert them into ALA which can then be used by the human body to make docosahexaenoic acid/eicosanoids that are beneficial to humans.<br><br> </p><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/File:CityU_HK_overview.jpgFile:CityU HK overview.jpg2014-10-17T13:19:10Z<p>Kenlaukit: uploaded a new version of &quot;File:CityU HK overview.jpg&quot;</p>
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<div></div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:07:25Z<p>Kenlaukit: </p>
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padding-right: 5%;<br />
text-align: justify;<br />
color: #333333;<br />
line-height: 2em;<br />
}<br />
<br />
p.content3{font-size:20px;<br />
padding-top: 0.5%;<br />
padding-left: 2.8%;<br />
padding-right: 5%;<br />
text-align: justify;<br />
color: #333333;<br />
line-height: 2em;<br />
}<br />
<br />
#main .col-md-12 {<br />
background: #F0EEEA;<br />
padding-bottom: 40px;<br />
border-radius: 20px;<br />
margin-bottom: 40px;<br />
}<br />
<br />
#main a {<br />
color: #3cc;}<br />
<br />
#main a:hover {<br />
color: orange;<br />
text-decoration:none;}<br />
<br />
table {<br />
border: none;<br />
margin-left: 1%;<br />
margin-right: 5%;<br />
}<br />
<br />
th {vertical-align: top;<br />
width: 8%;<br />
}<br />
<br />
td {vertical-align: centre;<br />
text-align: justify;<br />
font-size: 16px;<br />
color: #333333;<br />
}<br />
</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content3"> Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:07:11Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
<html><br />
<html lang="en"><br />
<br />
<style><br />
<br />
.table_form {<br />
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position: relative;<br />
border-radius:0px;<br />
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background-repeat: no-repeat;<br />
background-size: cover;<br />
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#title {<br />
color: #676767;<br />
text-align: center;<br />
margin-bottom: 40px;<br />
font-size: 2em;<br />
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font-weight: 700;<br />
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);<br />
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<br />
p.content3{font-size:20px;<br />
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#main .col-md-12 {<br />
background: #F0EEEA;<br />
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#main a {<br />
color: #3cc;}<br />
<br />
#main a:hover {<br />
color: orange;<br />
text-decoration:none;}<br />
<br />
table {<br />
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td {vertical-align: centre;<br />
text-align: justify;<br />
font-size: 16px;<br />
color: #333333;<br />
}<br />
</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content3"> Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:06:41Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
<html><br />
<html lang="en"><br />
<br />
<style><br />
<br />
.table_form {<br />
overflow: hidden;<br />
position: relative;<br />
border-radius:0px;<br />
line-height: 0px;<br />
background-image: url('#');<br />
background-repeat: no-repeat;<br />
background-size: cover;<br />
}<br />
<br />
#title {<br />
color: #676767;<br />
text-align: center;<br />
margin-bottom: 40px;<br />
font-size: 2em;<br />
font-family: "brandon-grotesque", sans-serif;<br />
font-weight: 700;<br />
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);<br />
padding: 0px;<br />
margin-top: 40px;<br />
letter-spacing: 1px;<br />
}<br />
<br />
#main h3 {<br />
font-size: 2em;<br />
color: #555;<br />
font-weight: bold;<br />
padding-left: 10px;<br />
padding-top: 10px;<br />
padding-bottom: 30px;<br />
<br />
}<br />
<br />
p.content1{font-size:20px;<br />
padding-top: 0.5%;<br />
padding-left: 1%;<br />
padding-right: 5%;<br />
text-align: justify;<br />
color: #333333;<br />
line-height: 2em;<br />
}<br />
p.content2{font-size:20px;<br />
padding-top: 0.5%;<br />
padding-left: 4%;<br />
padding-right: 5%;<br />
text-align: justify;<br />
color: #333333;<br />
line-height: 2em;<br />
}<br />
<br />
<br />
#main .col-md-12 {<br />
background: #F0EEEA;<br />
padding-bottom: 40px;<br />
border-radius: 20px;<br />
margin-bottom: 40px;<br />
}<br />
<br />
#main a {<br />
color: #3cc;}<br />
<br />
#main a:hover {<br />
color: orange;<br />
text-decoration:none;}<br />
<br />
table {<br />
border: none;<br />
margin-left: 1%;<br />
margin-right: 5%;<br />
}<br />
<br />
th {vertical-align: top;<br />
width: 8%;<br />
}<br />
<br />
td {vertical-align: centre;<br />
text-align: justify;<br />
font-size: 16px;<br />
color: #333333;<br />
}<br />
</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1"> Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:06:15Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
<html><br />
<html lang="en"><br />
<br />
<style><br />
<br />
.table_form {<br />
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#main .col-md-12 {<br />
background: #F0EEEA;<br />
padding-bottom: 40px;<br />
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<br />
#main a {<br />
color: #3cc;}<br />
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</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:06:01Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
<html><br />
<html lang="en"><br />
<br />
<style><br />
<br />
.table_form {<br />
overflow: hidden;<br />
position: relative;<br />
border-radius:0px;<br />
line-height: 0px;<br />
background-image: url('#');<br />
background-repeat: no-repeat;<br />
background-size: cover;<br />
}<br />
<br />
#title {<br />
color: #676767;<br />
text-align: center;<br />
margin-bottom: 40px;<br />
font-size: 2em;<br />
font-family: "brandon-grotesque", sans-serif;<br />
font-weight: 700;<br />
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);<br />
padding: 0px;<br />
margin-top: 40px;<br />
letter-spacing: 1px;<br />
}<br />
<br />
#main h3 {<br />
font-size: 2em;<br />
color: #555;<br />
font-weight: bold;<br />
padding-left: 10px;<br />
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padding-bottom: 30px;<br />
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line-height: 2em;<br />
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#main .col-md-12 {<br />
background: #F0EEEA;<br />
padding-bottom: 40px;<br />
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margin-bottom: 40px;<br />
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<br />
#main a {<br />
color: #3cc;}<br />
<br />
#main a:hover {<br />
color: orange;<br />
text-decoration:none;}<br />
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table {<br />
border: none;<br />
margin-left: 1%;<br />
margin-right: 5%;<br />
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th {vertical-align: top;<br />
width: 8%;<br />
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text-align: justify;<br />
font-size: 16px;<br />
color: #333333;<br />
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</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:05:51Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
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<html lang="en"><br />
<br />
<style><br />
<br />
.table_form {<br />
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background-size: cover;<br />
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<br />
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color: #676767;<br />
text-align: center;<br />
margin-bottom: 40px;<br />
font-size: 2em;<br />
font-family: "brandon-grotesque", sans-serif;<br />
font-weight: 700;<br />
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);<br />
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letter-spacing: 1px;<br />
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<br />
#main h3 {<br />
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<br />
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<br />
td {vertical-align: centre;<br />
text-align: justify;<br />
font-size: 16px;<br />
color: #333333;<br />
}<br />
</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:05:32Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
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<br />
<style><br />
<br />
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<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/achievement/medal_requirementTeam:CityU HK/achievement/medal requirement2014-10-17T13:04:57Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
<html><br />
<html lang="en"><br />
<br />
<style><br />
<br />
.table_form {<br />
overflow: hidden;<br />
position: relative;<br />
border-radius:0px;<br />
line-height: 0px;<br />
background-image: url('#');<br />
background-repeat: no-repeat;<br />
background-size: cover;<br />
}<br />
<br />
#title {<br />
color: #676767;<br />
text-align: center;<br />
margin-bottom: 40px;<br />
font-size: 2em;<br />
font-family: "brandon-grotesque", sans-serif;<br />
font-weight: 700;<br />
text-shadow: 0px 3px 2px rgba(0, 0, 0, 0.2);<br />
padding: 0px;<br />
margin-top: 40px;<br />
letter-spacing: 1px;<br />
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#main h3 {<br />
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#main .col-md-12 {<br />
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margin-bottom: 40px;<br />
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#main a {<br />
color: #3cc;}<br />
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#main a:hover {<br />
color: orange;<br />
text-decoration:none;}<br />
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table {<br />
border: none;<br />
margin-left: 1%;<br />
margin-right: 5%;<br />
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<br />
th {vertical-align: top;<br />
width: 8%;<br />
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<br />
td {vertical-align: centre;<br />
text-align: justify;<br />
font-size: 16px;<br />
color: #333333;<br />
}<br />
</style><br />
<br />
<body><br />
<br />
<div id="banner"><br />
<img src="https://static.igem.org/mediawiki/2014/c/c1/CityU_HK_Medal_banner.png" width=100%/><br />
</div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#996633>Bronze</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Team registration.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Successfully complete and submit iGEM 2014 Judging form.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the iGEM wiki and the Registry of Standard Biological Parts to share a Description of our team's project and our team's parts.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Present a Poster and Talk at the iGEM Jamboree.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Build up the attribution and acknowledgement page to clearly attribute the work done by student and others.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document three new standard BioBrick Part or Device used in our project and submit these parts to the iGEM Registry.<br><br />
Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a> &nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
<br />
<div id="main"><br />
<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#999999>Silver</font> Medal Requirements:</h3><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Experimentally validate that one new BioBrick Part and one Device of our own design and construction works as expected.</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Document the characterization of the parts in the Main Page section of that Part's/Device's Registry entry.<br>Parts number:<br><br />
<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a> &nbsp; [Received, Accepted] <br><br />
<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>&nbsp; [Received, Accepted] <br></p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content2">Submit these new parts to the iGEM Parts Registry</p></td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate three question encountered by our team, and describe how our team considered these) questions within our project.<br><br />
In our <a href="https://2014.igem.org/Team:CityU_HK/notebook/safety" target="_blank">Safety page</a>, we encountered several issue relating to our project: <b>General public Safety</b>, <b>Environmental Safety</b> & <b>What new risks might arise from our product growth</b>.</p></td><br />
</tr><br />
</table><br />
<br />
</div></div></div></div><br><br><br />
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<div class="container"><div class="row"><div class="col-md-12" ><br />
<h3> <font color=#E9C500>Gold</font> Medal Requirements:</h3><br />
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<table><br />
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<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Improve the the BioBricks <a href="http://parts.igem.org/Part:BBa_K925001" target="_blank">BBa_K925001</a> by codon-optimizing the sequence for E. coli. <br> The new improved parts : <a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a></p></td><br />
</tr><br />
</table><br />
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<table><br />
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<th><img src="https://static.igem.org/mediawiki/2014/6/69/CityU_HK_neon-tick-box.png" ></th><br />
<td><p class="content1">Provided assistance with the computer simulation of the “Nitrogenase System” project of the Chinese University of Hong Kong iGEM Team.</p></td><br />
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</table><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/File:CityU_HK_project_result_desaturase1.jpgFile:CityU HK project result desaturase1.jpg2014-10-17T12:35:24Z<p>Kenlaukit: uploaded a new version of &quot;File:CityU HK project result desaturase1.jpg&quot;</p>
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<div></div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:25:46Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
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<table><br />
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<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant <i>E. coli</i> clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in <i>E. coli</i>, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform <i>E. coli</i> TOP10 cells with (1) the <i>fadL-fadD</i> expression cassette, (2) the <i>‘tesA</i> expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in <i>E. coli</i> to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from <i>E. coli</i>, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br><br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</td><br />
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</table><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:24:18Z<p>Kenlaukit: </p>
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br><br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</td><br />
</tr><br />
</table><br />
</p><br />
</div><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/AcknowledgementTeam:CityU HK/Acknowledgement2014-10-17T08:23:13Z<p>Kenlaukit: </p>
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<div class="container"><br />
<div class="row" id="main"><br />
<h1 id="title">Acknowledgement</h1><br />
<p>This is the first time for City University of Hong Kong to participate in the iGEM competition. Therefore any form of assistance meant a lot for the team. Hereby, we would like to express our sincere gratitude to the following parties for their generous support!</p><br />
<br />
<h3>Financial support</h3><br />
<br />
<p><span>Office of Provost</span>, City University of Hong Kong, for funding registration and travel expenses.</p><br />
<p><span>Dr. Richard Y C KONG</span>, for purchasing and providing all the materials and equipment for the entire project.</p><br />
<br />
<h3>Material sponsorship</h3> <br />
<br />
<p><span>Invitrogen</span>, for providing two Pure Link(R) Quick Plasmid Miniprep Kits for free.</p><br />
<p><span>New England Biolabs</span>, for providing one BioBrick Assembly Kit for free.</p><br />
<p><span>Integrated DNA Technologies</span>, for taking 25% off for one Oligo purchase.</p><br />
<br />
<h3>Technical support</h3><br />
<br />
<p><span>Dr. Richard Y C KONG</span>, <b>Dr David S. K. Chiu</b>, <b>Dr Terrence C. K. Lau,</b>for guiding the team throughout the whole project.</p><br />
<p><span>Mr. LEUNG Tin Lung</span> and <b>Mr. CHEUNG Kwok Ming</b>, for providing the team with useful and invaluable background knowledge in molecular biology.</p><br />
<p><span>Mr. Ying Kwong Ho</span> and <span>Miss So Pui Yu</span>, for monitoring and technical advice on the experimental procedures.</p><br />
<p><span>Miss Alice K.Y. Chan</span>, for her assistance in the use of GC-MS.</p><br />
<p><span>Professor CHAN Ting Fung</span> (CUHK), for the introductory talk on iGEM and details about the iGEM competition.</p><br />
<p><span>Dr. Zhu Yuan</span> (Guangdong University of Finance and Economics), for helping and supervising our team on simulation for the collaboration with CUHK iGEM team.</p><br />
<p id="last-p"><span>Ms. Yu Wai Ping</span> ( Strewards MKMCF Ma Ko Pan Memorial College), for helping our team organize the talk on human practice in her college.</p><br />
</div><br />
</div><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/AcknowledgementTeam:CityU HK/Acknowledgement2014-10-17T08:22:44Z<p>Kenlaukit: </p>
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<body><br />
<div class="container"><br />
<div class="row" id="main"><br />
<h1 id="title">Acknowledgement</h1><br />
<p>This is the first time for City University of Hong Kong to participate in the iGEM competition. Therefore any form of assistance meant a lot for the team. Hereby, we would like to express our sincere gratitude to the following parties for their generous support!</p><br />
<br />
<h3>Financial support</h3><br />
<br />
<p><span>Office of Provost</span>, City University of Hong Kong, for funding registration and travel expenses.</p><br />
<p><span>Dr. Richard Y C KONG</span>, for purchasing and providing all the materials and equipment for the entire project.</p><br />
<br />
<h3>Material sponsorship</h3> <br />
<br />
<p><span>Invitrogen</span>, for providing two Pure Link(R) Quick Plasmid Miniprep Kits for free.</p><br />
<p><span>New England Biolabs</span>, for providing one BioBrick Assembly Kit for free.</p><br />
<p><span>Integrated DNA Technologies</span>, for taking 25% off for one Oligo purchase.</p><br />
<br />
<h3>Technical support</h3><br />
<br />
<p><span>Dr. Richard Y C KONG</span>, <b>Dr David S. K. Chiu</b>, <b>Dr Terrence C. K. Lau,</b>for guiding the team throughout the whole project.</p><br />
<p><span>Mr. LEUNG Tin Lung</span> and <b>Mr. CHEUNG Kwok Ming</b>, for providing the team with useful and invaluable background knowledge in molecular biology.</p><br />
<p><span>Mr. Ying Kwong Ho</span> and <span>Miss So Pui Yu</span>, for monitoring and technical advice on the experimental procedures.</p><br />
<p><span>Miss Alice K.Y. Chan</span>, for her assistance in the use of GC-MS.</p><br />
<p><span>Professor CHAN Ting Fung</span> (CUHK), for the introductory talk on iGEM and details about the iGEM competition.</p><br />
<p><span>Dr. Zhu Yuan</span> (Guangdong University of Finance and Economics), for helping and supervising our team on simulation for the collaboration with CUHK iGEM team.</p><br />
<p id="last-p"><span>Ms. Yu Wai Ping</span> ( Strewards MKMCF Ma Ko Pan Memorial College), for helping our team organize the talk on human practice in her college.</p><br />
</div><br />
</div><br />
<br />
</body><br />
<br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/AcknowledgementTeam:CityU HK/Acknowledgement2014-10-17T08:22:05Z<p>Kenlaukit: </p>
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#title {<br />
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}<br />
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}<br />
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</style><br />
</head><br />
<br />
<br />
<body><br />
<div class="container"><br />
<div class="row" id="main"><br />
<h1 id="title">Acknowledgement</h1><br />
<p>This is the first time for City University of Hong Kong to participate in the iGEM competition. Therefore any form of assistance meant a lot for the team. Hereby, we would like to express our sincere gratitude to the following parties for their generous support!</p><br />
<br />
<h3>Financial support</h3><br />
<br />
<p><span>Office of Provost</span>, City University of Hong Kong, for funding registration and travel expenses.</p><br />
<p><span>Dr. Richard Y C KONG</span>, for purchasing and providing all the materials and equipment for the entire project.</p><br />
<br />
<h3>Material sponsorship</h3> <br />
<br />
<p><span>Invitrogen</span>, for providing two Pure Link(R) Quick Plasmid Miniprep Kits for free.</p><br />
<p><span>New England Biolabs</span>, for providing one BioBrick Assembly Kit for free.</p><br />
<p><span>Integrated DNA Technologies</span>, for taking 25% off for one Oligo purchase.</p><br />
<br />
<h3>Technical support</h3><br />
<br />
<p><span>Dr. Richard Y C KONG</span>, <b>Dr David S. K. Chiu</b>, <b>Dr Terrence C. K. Lau,</b>for guiding the team throughout the whole project.</p><br />
<p><span>Mr. LEUNG Tin Lung</span> and <b>Mr. CHEUNG Kwok Ming</b>, for providing the team with useful and invaluable background knowledge in molecular biology.</p><br />
<p><span>Mr. Ying Kwong Ho</span> and <span>Miss So Pui Yu</span>, for monitoring and technical advice on the experimental procedures.</p><br />
<p><span>Miss Alice K.Y. Chan</span>, for her assistance in the use of GC-MS.</p><br />
<p><span>Professor CHAN Ting Fung</span> (CUHK), for the introductory talk on iGEM and details about the iGEM competition.</p><br />
<p><span>Dr. Zhu Yuan</span> (Guangdong University of Finance and Economics), for helping and supervising our team on simulation for the collaboration with CUHK iGEM team.</p><br />
<p id="last-p"><span>Ms. Yu Wai Ping</span> ( Strewards MKMCF Ma Ko Pan Memorial College), for helping our team organize the talk on human practice in her college.</p><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/AcknowledgementTeam:CityU HK/Acknowledgement2014-10-17T08:21:22Z<p>Kenlaukit: </p>
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<h1 id="title">Acknowledgement</h1><br />
<p>This is the first time for City University of Hong Kong to participate in the iGEM competition. Therefore any form of assistance meant a lot for the team. Hereby, we would like to express our sincere gratitude to the following parties for their generous support!</p><br />
<br />
<h3>Financial support</h3><br />
<br />
<p><span>Office of Provost</span>, City University of Hong Kong, for funding registration and travel expenses.</p><br />
<p><span>Dr. Richard Y C KONG</span>, for purchasing and providing all the materials and equipment for the entire project.</p><br />
<br />
<h3>Material sponsorship</h3> <br />
<br />
<p><span>Invitrogen</span>, for providing two Pure Link(R) Quick Plasmid Miniprep Kits for free.</p><br />
<p><span>New England Biolabs</span>, for providing one BioBrick Assembly Kit for free.</p><br />
<p><span>Integrated DNA Technologies</span>, for taking 25% off for one Oligo purchase.</p><br />
<br />
<h3>Technical support</h3><br />
<br />
<p><span>Dr. Richard Y C KONG</span>, <b>Dr David S. K. Chiu</b>, <b>Dr Terrence C. K. Lau,</b>for guiding the team throughout the whole project.</p><br />
<p><span>Mr. LEUNG Tin Lung</span> and <b>Mr. CHEUNG Kwok Ming</b>, for providing the team with useful and invaluable background knowledge in molecular biology.</p><br />
<p><span>Mr. Ying Kwong Ho</span> and <span>Miss So Pui Yu</span>, for monitoring and technical advice on the experimental procedures.</p><br />
<p><span>Miss Alice K.Y. Chan</span>, for her assistance in the use of GC-MS.</p><br />
<p><span>Professor CHAN Ting Fung</span> (CUHK), for the introductory talk on iGEM and details about the iGEM competition.</p><br />
<p><span>Dr. Zhu Yuan</span> (Guangdong University of Finance and Economics), for helping and supervising our team about simulation on the collaboration with CUHK iGEM team.</p><br />
<p id="last-p"><span>Ms. Yu Wai Ping</span> ( Strewards MKMCF Ma Ko Pan Memorial College), for helping our team organize the talk on human practice in her college.</p><br />
</div><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/TemplateTeam:CityU HK/Template2014-10-17T08:15:41Z<p>Kenlaukit: </p>
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</html></div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:15:10Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
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<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
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</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br><br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
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<br />
<br />
<br><br />
<br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:14:57Z<p>Kenlaukit: </p>
<hr />
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br><br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:14:33Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</td><br />
</tr><br />
</table><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:14:26Z<p>Kenlaukit: </p>
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<br />
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="8%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</td><br />
</tr><br />
</table><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:14:13Z<p>Kenlaukit: </p>
<hr />
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</td><br />
</tr><br />
</table><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:12:36Z<p>Kenlaukit: </p>
<hr />
<div>{{:Team:CityU_HK/Template}}<br />
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<br />
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="reference" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="5%"> Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
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</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:12:08Z<p>Kenlaukit: </p>
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<br />
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="reference" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="5%"> Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:11:14Z<p>Kenlaukit: </p>
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<br />
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="reference" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="5%"> Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:09:40Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="reference" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="5%"> <div id="main">Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a></div><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:08:47Z<p>Kenlaukit: </p>
<hr />
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="reference" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="8%"> <div>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a></div><br />
</p><br />
</div><br />
<br />
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<br><br><br />
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</body><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:07:57Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> <div>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a></div><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:06:59Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<p id="reference"><b>References:</b><br><br><br />
<img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="20%"> <div>Icon made by <a href="http://www.freepik.com" title="Freepik">Freepik</a> from <a href="http://www.flaticon.com" title="Flaticon">www.flaticon.com</a> is licensed under <a href="http://creativecommons.org/licenses/by/3.0/" title="Creative Commons BY 3.0">CC BY 3.0</a></div><br />
</p><br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:05:05Z<p>Kenlaukit: </p>
<hr />
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:04:47Z<p>Kenlaukit: </p>
<hr />
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="5">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:04:38Z<p>Kenlaukit: </p>
<hr />
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<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0 size="6">Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T08:03:34Z<p>Kenlaukit: </p>
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Replication of quantitative RT-PCR and GC-MS experiments.</font></b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3.</font> </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Cotransformation of TOP10 cells with three different gene cassettes.</font> </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b><font color=#40e0d0>Studies on efficiency of ALA exportation by Fit Coli strain.</font> </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:58:24Z<p>Kenlaukit: </p>
<hr />
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Replication of quantitative RT-PCR and GC-MS experiments.</b><br><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:57:41Z<p>Kenlaukit: </p>
<hr />
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<body><br />
<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><h2>Replication of quantitative RT-PCR and GC-MS experiments.</h2><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:56:35Z<p>Kenlaukit: </p>
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<br />
<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><h2>Replication of quantitative RT-PCR and GC-MS experiments.</h2><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:56:17Z<p>Kenlaukit: </p>
<hr />
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<body><br />
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<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><h2>Replication of quantitative RT-PCR and GC-MS experiments.</h2><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:52:23Z<p>Kenlaukit: </p>
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<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Replication of quantitative RT-PCR and GC-MS experiments.</b><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:52:05Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Replication of quantitative RT-PCR and GC-MS experiments.</b><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:51:38Z<p>Kenlaukit: </p>
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<div id="main"><br />
<h1 id="title"> Future plan </h1><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Replication of quantitative RT-PCR and GC-MS experiments.</b><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
</td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
</td><br />
</tr><br />
</table><br />
<br />
<table><br />
<tr><br />
<th><img class="displayed" src="https://static.igem.org/mediawiki/2014/0/0a/CityU_HK_futureplan_telescope.png" width="50%"> </th><br />
<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the <a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf" target="_blank">MazF protein</a> (a stable toxin) that initiates program death in the cells will also be tested.<br />
</td><br />
</tr><br />
</table><br />
<br />
<br />
</div><br />
<br />
<br />
<br><br><br />
<br />
</body><br />
</html><br />
{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/result_tesATeam:CityU HK/project/result tesA2014-10-17T07:50:29Z<p>Kenlaukit: </p>
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<ul><br />
<li><a href='https://2014.igem.org/Team:CityU_HK/project/result'><span>Result Overview</span></a></li><br />
<li><a href='https://2014.igem.org/Team:CityU_HK/project/result_fad'><span>FadD & FadL Module</span></a></li><br />
<li><a href='https://2014.igem.org/Team:CityU_HK/project/result_tesA'><span>'TesA Module</span></a></li><br />
<li class='last'><a href='https://2014.igem.org/Team:CityU_HK/project/result_desaturase'><span>Desaturase Module</span></a></li><br />
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<div id="main"><br />
<h1 id="title"> Overexpression of <i>‘tesA</i> Gene and Effect on Conversion of Fatty acyl-CoA to Fatty acid </h1><br />
<br />
<h2 class="sub">Overview :</h2><br />
<br />
<p class="content">We aimed to overexpress the <i>‘tesA</i> gene (which codes for thioesterase I) in <i>E. coli</i> to (1) enhance the conversion of fatty acyl-CoA to free fatty acid, and (2) bypass the β-oxidation pathway for fatty acid metabolism. To facilitate this strategy, we have subcloned the <i>‘tesA</i> coding sequence downstream of the PBAD promoter in the pSB1C3 vector. <i>E. coli</i> TOP10 cells (Invitrogen) was used as the host to overexpress <i>‘tesA</i>. Expression of the <i>‘tesA</i> gene was measured by quantitative RT-PCR and the effect of ‘TesA overexpression (leading to a possible increase in thioesterase I activity) was indirectly tested by determining the oleic acid concentrations in control and <i>‘tesA</i> recombinant <i>E. coli</i> cells by GC-MS.</p><br><br><br />
<br />
<h2 class="sub">Results :</h2><br />
<p class="content">Quantitative real-time PCR analyses showed that <i>‘tesA</i> mRNA overexpression was observed only in recombinant <i>E. coli</i> Top10 cells and not in the DH5α host. Expression of ‘<i>tesA</i> was 2.5-fold higher in the recombinant <i>‘tesA E. coli</i> TOP10 cells than the control cells (Figure 1). </p><br><br />
<br />
<img class="displayed" src="https://static.igem.org/mediawiki/2014/b/b3/CityU_HK_project_result_tesA1.png" width="35%"><br><br />
<br />
<table><br />
<tr><br />
<th>Figure 1. </th><br />
<td><b>Quantitative RT-PCR of <i>‘tesA</i> expression in <i>E. coli</i> DH5α and TOP10 host cells.</b> Expression of the <i>‘tesA</i> gene was determined in control and <i>‘tesA</i> recombinant <i>E.coli</i> DH5α and <i>E. coli</i> TOP10 cells. <i>E. coli</i> cells were cultured overnight in LB + oleic acid (3.5 mM) medium. Cells were harvested for total RNA extraction and lipid extraction for GC-MS analysis. </td><br />
</tr><br />
</table><br><br />
<br />
<p class="content">For the fatty acid analysis in <i>E. coli</i> TOP10 cells, the levels of both oleic acid and palmitic acid were elevated in recombinant <i>‘tesA</i> TOP10 cells (but not recombinant DH5α cells) as compared to the control host cells (Figures 2A and 2B). The results suggested that the increase in oleic acid and palmitic acid levels in the recombinant TOP10 cells may be due to increased expression of the <i>‘tesA</i> gene (and hence thioesterase I activity) in these <i>E. coli</i> cells. However, due to time constraint, these experiments were performed only once, and further replicates will need to be carried out in future studies in order to determine the statistical significance of the data.</p><br />
<br />
<br />
<br><img class="displayed" src="https://static.igem.org/mediawiki/2014/a/a9/CityU_HK_project_result_tesA2.png" width="35%"><br><br />
<img class="displayed" src="https://static.igem.org/mediawiki/2014/a/a4/CityU_HK_project_result_tesA3.png" width="35%"><br><br />
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<table><br />
<tr><br />
<th>Figure 2. </th><br />
<td><b>Intracellular levels of oleic acid and palmitic acid in recombinant <i>‘tesA</i> and control <i>E. coli</i> cells.</b> (A). Oleic acid concentration in control DH5α / TOP10 cells (<font color=#FFC000>Yellow</font>), and <i>‘tesA</i> recombinant DH5α /TOP10 cells (<font color=#A5A5A5>Gray</font>). (B). Palmitic acid concentration in the control DH5α / TOP10 cells (<font color=#FFC000>Yellow</font>), and <i>‘tesA</i> recombinant DH5α /TOP10 cells (<font color=#A5A5A5>Gray</font>). </td><br />
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{{:Team:CityU_HK/Template/footer}}</div>Kenlaukithttp://2014.igem.org/Team:CityU_HK/project/futureplanTeam:CityU HK/project/futureplan2014-10-17T07:49:35Z<p>Kenlaukit: </p>
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<h1 id="title"> Future plan </h1><br />
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<td><b>Replication of quantitative RT-PCR and GC-MS experiments.</b><br><br />
Due to time constraint, the RT-PCR and GC-MS experiments on the different recombinant E. coli clones were performed only once, and will have to be repeated in future studies in order to verify the statistical significance of the data.<br />
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<td><b>Construction of Δ9-Δ12-Δ15 desaturase gene cluster downstream of the LacI promoter in pSB1C3. </b><br><br />
For overexpression studies of the Δ9-Δ12-Δ15 desaturase gene cluster in E. coli, we will repeat subcloning of the desaturase gene cluster into pSB1C3 to create a regulatable expression system for ALA production. <br />
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<td><b>Cotransformation of TOP10 cells with three different gene cassettes. </b><br><br />
Attempts will be made to co-transform E. coli TOP10 cells with (1) the fadL-fadD expression cassette, (2) the ‘tesA expression cassette, and (3) the Δ9-Δ12-Δ15 desaturase gene cassette to construct a Fit Coli strain. Expression of the gene cassettes will be analysed by qRT-PCR, whilst fatty acid uptake and ALA production will be monitored by GC-MS analyses.<br />
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<td><b>Studies on efficiency of ALA exportation by Fit Coli strain. </b><br><br />
Because unsaturated fatty acids are expensive sources of carbon and energy from the perspective of a cell, and an ALA export protein may not be present in E. coli to facilitate ALA export for human uptake, we will consider a pH-induced autolysis system to facilitate extracellular ALA release. When bacteria reach a certain section of the gut of a particular pH, the cell will open up so the human gut may gain access to the useful ALA. Alternatively, a bile salt-inducible system to activate yet another engineered gene from E. coli, which encodes the MazF protein (a stable toxin) that initiates program death in the cells will also be tested (http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2005.04956.x/pdf).<br />
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