http://2014.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=50&target=Michaelc1618&year=&month=2014.igem.org - User contributions [en]2024-03-29T13:24:22ZFrom 2014.igem.orgMediaWiki 1.16.5http://2014.igem.org/File:Quartzy_screenshot.PNGFile:Quartzy screenshot.PNG2015-02-03T02:29:32Z<p>Michaelc1618: </p>
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<div></div>Michaelc1618http://2014.igem.org/Team:UCLA/AttributionsTeam:UCLA/Attributions2015-02-03T02:28:49Z<p>Michaelc1618: </p>
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<h2>Acknowledgements</h2><br />
<p><strong><i>Advising:</i></strong><br /><br />
Dr. Sriram Kosuri<br /><br />
Dr. David Breslauer<br /><br />
Dr. Mark Arbing<br /><br />
Dr. Christina Agapakis<br /><br />
Keegan Owsley<br /><br />
David Yao<br /><br/><br />
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<strong><i>Lab Space:</i></strong><br /><br />
Dr. Foad Mashayekhi<br /><br />
Dr. Dino DiCarlo<br /><br />
Garrett Mosley<br /><br />
UCLA Department of Bioengineering<br /><br /><br />
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<strong><i>Supplies and Assistance:</i></strong><br /><br />
Dr. Kayvan Niazi<br /><br />
Eisenberg Lab<br /><br />
Brad Roa<br /><br />
Bryant Cheng<br /><br />
Bradley Pavy<br /><br /><br />
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<strong><i>Financial Support:</i></strong><br /><br />
UCLA Department of Bioengineering<br /><br />
UCLA Department of Chemistry and Biochemistry<br /><br />
UCLA Department of Microbiology, Immunology, and Molecular Genetics<br /><br />
Refactored Materials<br /><br />
Office of the Dean of Undergraduate Education<br /><br />
Tama Hasson, Vice Provost for Undergraduate Research<br /><br /><br />
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<strong><i>Art | Sci Collaboration:</i></strong><br /><br />
Dr. Victoria Vesna<br /><br />
Mick Lorusso<br /><br /><br />
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<strong><i>Administrative Support:</i></strong><br /><br />
Stacey Tran<br /><br />
Brian Lee<br /><br /><br />
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[[File:Jamboree2014 Overhead.jpg]]</div>Michaelc1618http://2014.igem.org/Team:UCLA/AttributionsTeam:UCLA/Attributions2015-02-03T02:28:20Z<p>Michaelc1618: </p>
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<h2>Acknowledgements</h2><br />
<p><strong><i>Advising:</i></strong><br /><br />
Dr. Sriram Kosuri<br /><br />
Dr. David Breslauer<br /><br />
Dr. Mark Arbing<br /><br />
Dr. Christina Agapakis<br /><br />
Keegan Owsley<br /><br />
David Yao<br /><br/><br />
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<br />
<strong><i>Lab Space:</i></strong><br /><br />
Dr. Foad Mashayekhi<br /><br />
Dr. Dino DiCarlo<br /><br />
Garrett Mosley<br /><br />
UCLA Department of Bioengineering<br /><br /><br />
<br />
<strong><i>Supplies and Assistance:</i></strong><br /><br />
Dr. Kayvan Niazi<br /><br />
Eisenberg Lab<br /><br />
Brad Roa<br /><br />
Bryant Cheng<br /><br />
Bradley Pavy<br /><br /><br />
<br />
<br />
<strong><i>Financial Support:</i></strong><br /><br />
UCLA Department of Bioengineering<br /><br />
UCLA Department of Chemistry and Biochemistry<br /><br />
UCLA Department of Microbiology, Immunology, and Molecular Genetics<br /><br />
Refactored Materials<br /><br />
Office of the Dean of Undergraduate Education<br /><br />
Tama Hasson, Vice Provost for Undergraduate Research<br /><br /><br />
<br />
<strong><i>Art | Sci Collaboration:</i></strong><br /><br />
Dr. Victoria Vesna<br /><br />
Mick Lorusso<br /><br /><br />
<br />
<strong><i>Administrative Support:</i></strong><br /><br />
Stacey Tran<br /><br />
Brian Lee<br /><br /><br />
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[[Image:Jamboree2014 Overhead.jpg]]</div>Michaelc1618http://2014.igem.org/Giant_Jamboree/PracticeSessionsGiant Jamboree/PracticeSessions2014-10-30T11:41:12Z<p>Michaelc1618: </p>
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<h3> PROGRAM</h3><br />
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<li><a style="color: #49b677" href="https://2014.igem.org/Giant_Jamboree/Projects"><p> <b> Projects </p> </b> </a> </li><br />
<li><a style="color: #49b677" href="https://2014.igem.org/Giant_Jamboree/Booklet"><p><b>Booklet </p> </b></a></li><br />
<li><a style="color: #49b677" href="https://2014.igem.org/Giant_Jamboree/Schedule"><p><b> Schedule </p></b> </a> </li><br />
<li><a style="color: #49b677" href="https://2014.igem.org/Giant_Jamboree/SpecialEvents"><p><b> Special Events</p></b> </a> </li><br />
<li><a style="color: #49b677" href="https://2014.igem.org/Giant_Jamboree/Handbook"> <p> <b>Participant Handbook </b></p> </a> </li><br />
<li><a style="color: #49b677" href="https://2014.igem.org/Giant_Jamboree/PracticeSessions"> <p> <b>Practice Sessions </b> </p></a> </li><br />
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<h3>Practice Sessions </h3><br />
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<p>Use this sign-up sheet to sign up for a practice session slot on Thursday night (October 30) to practice your talk. Note that there will NOT be any A/V (audio/visual) support on staff. All rooms will be unlocked and you should use them and leave them as you found them. Be sure to bring necessary computer equipment with you, such as chargers and adapters, as these will not be provided.</p><br />
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<p>There are a limited number of time slots available on a first-come first-serve basis so please only choose one slot. We cannot match the room that you will ultimately give your presentation in with the practice room (please see the campus map for building locations). This should, however, give you a chance to practice your talk in a new environment. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time.</p><br />
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<p>Use the wiki <a style="color: #49b677" href="https://2014.igem.org/wiki/index.php?title=Giant_Jamboree/PracticeSessions&action=edit">edit button </a> to add your team to the schedule (the markup is located at the bottom of the page). Additional rooms may be added in the coming weeks.</p><br />
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<p><i>** Please note that rooms 310 and 313 will not have any A/V.</i></p><br />
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<th width="85px"> Room/Hour </th><br />
<th width="85px">207</th><br />
<th width="85px">208</th><br />
<th width="85px">210</th><br />
<th width="85px">302</th><br />
<th width="85px">304</th><br />
<th width="85px">306</th><br />
<th width="85px">309</th><br />
<th width="85px">311</th><br />
<th width="85px">312</th><br />
<th width="85px">310 **</th><br />
<th width="85px">313 **</th><br />
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<td> 2:00 - 2:30 </td><br />
<td> SDU Denmark </td><br />
<td> NEAU-Harbin </td><br />
<td> Aalto-Helsinki </td><br />
<td> RHIT </td><br />
<td> HNU_China </td><br />
<td> Exeter </td><br />
<td> Aberdeen_Scotland</td><br />
<td> KAIT_JAPAN </td><br />
<td> Paris Bettencourt </td><br />
<td> J1 </td><br />
<td> K1 </td><br />
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<td> 2:30 - 3:00 </td><br />
<td> Berlin </td><br />
<td> ITESM CEM </td><br />
<td> Goettingen</td><br />
<td> Cambridge-JIC </td><br />
<td> Evry </td><br />
<td> Szu-China </td><br />
<td> Warwick </td><br />
<td> KIT-Kyoto</td><br />
<td> XMU-China </td><br />
<td> NCSU GES </td><br />
<td> K2 </td><br />
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<td> 3:00 - 3:30 </td><br />
<td> Glasgow </td><br />
<td> Wageningen UR </td><br />
<td> UFAM_Brazil </td><br />
<td> Oxford </td><br />
<td> NJAU_China </td><br />
<td> ITB Indonesia </td><br />
<td> SJTU-Software </td><br />
<td> USTC-Software </td><br />
<td> METU_Turkey </td><br />
<td> HFUT-China </td><br />
<td> UCLA </td><br />
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<td> 3:30 - 4:00 </td><br />
<td> NYMU-Taipei </td><br />
<td> UCSD </td><br />
<td> CityU_HK </td><br />
<td> UCSC </td><br />
<td> NJU-QIBEBT </td><br />
<td> Jilin-China</td><br />
<td> Nagahama</td><br />
<td> Georgia Tech </td><br />
<td> NTU-Taida </td><br />
<td> J4 </td><br />
<td> K4 </td><br />
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<td> 4:00 - 4:30 </td><br />
<td> USyd-Australia </td><br />
<td> Paris_Saclay </td><br />
<td> OUC-China </td><br />
<td> Sheffield </td><br />
<td> Macquarie_Australia </td><br />
<td> Sumbawagen </td><br />
<td> Gifu </td><br />
<td> Valencia Biocampus </td><br />
<td> Pitt </td><br />
<td> J5 </td><br />
<td> K5 </td><br />
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<td> 4:30 - 5:00 </td><br />
<td> Tokyo_Tech</td><br />
<td> Nanjing-China </td><br />
<td> BIT-China </td><br />
<td> HZAU-China </td><br />
<td> Gaston_Day_School </td><br />
<td> TU Eindhoven </td><br />
<td> Kyoto </td><br />
<td> Edinburgh </td><br />
<td> Aix-Marseille </td><br />
<td> HokkaidoU_Japan </td><br />
<td> K6 </td><br />
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<td> 5:30 - 6:00 </td><br />
<td> Tsinghua </td><br />
<td> UESTC-Software</td><br />
<td> Groningen </td><br />
<td> Valencia_UPV </td><br />
<td> TU_Darmstadt </td><br />
<td> Korea_U_Seoul </td><br />
<td> Saarland </td><br />
<td> UT-Tokyo </td><br />
<td> Virtus-Parva_Mexico </td><br />
<td> UMayor </td><br />
<td> K7 </td><br />
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<td> 6:00 - 6:30 </td><br />
<td> ETH Zurich </td><br />
<td> Braunschweig </td><br />
<td> Bielefeld-CeBiTec </td><br />
<td> Aachen </td><br />
<td> Hannover </td><br />
<td> UST-Beijing </td><br />
<td> Uppsala </td><br />
<td> MIT </td><br />
<td> Austin_Texas </td><br />
<td> UGA-Georgia </td><br />
<td> LiTH </td><br />
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<td> 6:30 - 7:00 </td><br />
<td> Linköping University </td><br />
<td> UMaryland </td><br />
<td> ZJU-China</td><br />
<td> INSA - Lyon </td><br />
<td> ATOMS Turkiye </td><br />
<td> Minnesota </td><br />
<td> AHUT_China</td><br />
<td> Michigan </td><br />
<td> UESTC-China </td><br />
<td> J9 </td><br />
<td> Imperial </td><br />
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<td> 7:00 - 7:30 </td><br />
<td> SYSU-China </td><br />
<td> Hong_Kong-CUHK </td><br />
<td> Calgary </td><br />
<td> UI-Indonesia </td><br />
<td> uOttawa </td><br />
<td> UFMG_Brazil </td><br />
<td> Peking </td><br />
<td> BNU-China </td><br />
<td> TCU-Taiwan </td><br />
<td> UCL </td><br />
<td> BIOSINT_Mexico </td><br />
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<td> 7:30 - 8:00 </td><br />
<td> Caltech </td><br />
<td> Toulouse </td><br />
<td> BostonU </td><br />
<td> Dundee </td><br />
<td> UCSF_UCB </td><br />
<td> Nevada </td><br />
<td> Missouri_Miners </td><br />
<td> Carnegie_Mellon </td><br />
<td> NCTU_Formosa </td><br />
<td> Toronto </td><br />
<td> Lethbridge </td><br />
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<td> 8:00 - 8:30 </td><br />
<td> IIT Delhi</td><br />
<td> NU_Kazakhstan </td><br />
<td> WLC-Milwaukee </td><br />
<td> Warsaw </td><br />
<td> Georgia State </td><br />
<td> SUSTC-Shenzhen </td><br />
<td> Michigan_Software </td><br />
<td> Tec-Monterrey </td><br />
<td> Zamorano </td><br />
<td> SJTU-BioX-Shanghai </td><br />
<td> BYU Provo</td><br />
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<td> 8:30 - 9:00 </td><br />
<td> Freiburg </td><br />
<td> LMU-Munich </td><br />
<td> WHU-China </td><br />
<td> Technion_Israel </td><br />
<td> Cornell </td><br />
<td> Utah State </td><br />
<td> BGU-Israel</td><br />
<td> Colombia </td><br />
<td> Washington </td><br />
<td> SF BAY AREA DIYbio </td><br />
<td> UiOslo_Norway </td><br />
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<p>** Please note that rooms 310 and 313 will not have any A/V.</p><br />
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{{MainPage2014/Footer}}</div>Michaelc1618http://2014.igem.org/Team:UCLA/AttributionsTeam:UCLA/Attributions2014-10-18T03:37:33Z<p>Michaelc1618: </p>
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<h2>Acknowledgements</h2><br />
<p><strong><i>Advising:</i></strong><br /><br />
Dr. Sriram Kosuri<br /><br />
Dr. David Breslauer<br /><br />
Dr. Mark Arbing<br /><br />
Dr. Christina Agapakis<br /><br />
Keegan Owsley<br /><br />
David Yao<br /><br/><br />
<br />
<br />
<strong><i>Lab Space:</i></strong><br /><br />
Dr. Foad Mashayekhi<br /><br />
Dr. Dino DiCarlo<br /><br />
Garrett Mosley<br /><br />
UCLA Department of Bioengineering<br /><br /><br />
<br />
<strong><i>Supplies and Assistance:</i></strong><br /><br />
Dr. Kayvan Niazi<br /><br />
Eisenberg Lab<br /><br />
Brad Roa<br /><br />
Bryant Cheng<br /><br />
Bradley Pavy<br /><br /><br />
<br />
<br />
<strong><i>Financial Support:</i></strong><br /><br />
UCLA Department of Bioengineering<br /><br />
UCLA Department of Chemistry and Biochemistry<br /><br />
UCLA Department of Microbiology, Immunology, and Molecular Genetics<br /><br />
Refactored Materials<br /><br />
Office of the Dean of Undergraduate Education<br /><br />
Tama Hasson, Vice Provost for Undergraduate Research<br /><br /><br />
<br />
<strong><i>Art | Sci Collaboration:</i></strong><br /><br />
Dr. Victoria Vesna<br /><br />
Mick Lorusso<br /><br /><br />
<br />
<strong><i>Administrative Support:</i></strong><br /><br />
Stacey Tran<br /><br />
Brian Lee<br /><br /><br />
<br />
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<h1>Processing Silk</h1><br />
<h2>Preparation for Silk Materials</h2><br />
<p><b>Step 1: Degumming</b></p><br />
<p>Silk is comprised of two main proteins, fibroin and sericin. Fibroin is the structural protein of the silk and our protein of interest. Sericin serves as the ‘glue’ of the silk. Degumming separates and removes sericin and is an essential preparation step before dissolving the silk. We found that commercially degummed silk was not properly degummed for our purposes and this step had to be carried out in-lab. </p><br />
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<figure style= "margin: 10px; float: right;" align="middle"><br />
<a href = "https://static.igem.org/mediawiki/2014/7/73/Degummed_silk_top.jpg"><br />
<img src= "https://static.igem.org/mediawiki/2014/7/73/Degummed_silk_top.jpg" width = 25% height = 25% /></a><br />
<figcaption style="margin: auto;">Same sample of silk after degumming.</figcaption></figure><br />
<br />
<p><b>Step 2: Solubilization</p></b><br />
<p>Getting significant amounts of silk (>0.25 grams) into solution requires the use of lithium bromide <sup><a href="#references">[1]</a></sup>. Protocols with less hazardous chemicals were attempted, yet none were able to reproduce results obtained using lithium bromide. The silk is cut into small pieces and dissolved in a scintillation vial at 60 degrees Celcius for 4 hours. </p><br />
<br />
<figure style= "margin: 10px; float: right;" align="middle"><br />
<a href = "https://static.igem.org/mediawiki/2014/1/10/LiBr_soluble_silk.jpg"><br />
<img src= "https://static.igem.org/mediawiki/2014/1/10/LiBr_soluble_silk.jpg" width = 25% height = 25% /></a><br />
<figcaption style="margin: auto;">Silk solubilized in a solution of lithium bromide.</figcaption></figure><br />
<br />
<p><b>Step 3: Dialysis</b></p><br />
<p>Dialysis of the Fibroin-Lithium Bromide solution removes the lithium bromide, and results in an aqueous solution of the fibroin protein<sup><a href="#references">[1]</a></sup>. This solution can then be processed further in a variety of ways to achieve various structures and conformations of the silk protein.</p><br />
<br />
<figure style= "margin: 10px; float: right;" align="middle"><br />
<a href = "https://static.igem.org/mediawiki/2014/8/88/Dialyzed_silk.jpg"><br />
<img src= "https://static.igem.org/mediawiki/2014/8/88/Dialyzed_silk.jpg" width = 25% height = 25% /></a><br />
<figcaption style="margin: auto;">Silk solution after dialysis.</figcaption></figure><br />
<br />
<p><b>Material - Silk Films</b></p><br />
<p>Silk films are the simplest way of processing the silk. The silk can be processed directly after dialysis. Dialyzed silk is simply dehydrated and the remaining silk aggregates into a film (protocol link). This simplicity may prove to be an asset as researchers investigate these films for next-generation medicine. The biodegradability of the films allows for technology like conformal electronics to be bio-integrated<sup><a href="#references">[2]</a></sup>.</p><br />
<br />
<figure style= "margin: 10px; float: right;" align="middle"><br />
<a href = "https://static.igem.org/mediawiki/2014/8/8f/Silk_film.jpg"><br />
<img src= "https://static.igem.org/mediawiki/2014/8/8f/Silk_film.jpg" width = 25% height = 25% /></a><br />
<figcaption style="margin: auto;">Silk film created by drying dialyzed silk overnight.</figcaption></figure><br />
<br />
<br />
<p><b>Material - Hydrogels</b></p><br />
<p>A silk hydrogel is a general term for a variety of ‘gel’ structures that can be created from silk. We chose to create a pH induced gel: the nature of this gel creates a chemically alterable microenvironment that can be tailored to optimize conditions for pharmaceuticals, tissues, or other biological agents. Biomedical applications of this technology could range from pain relievers integrated into a suture to tissue wound sealing.</p><br />
<br />
<p><b>Optional Processing: Lyophilization</p></b><br />
<p>Water can be completely removed from the dialyzed silk by the process of lyophilization, or freeze-drying. In this process, the solution is frozen by liquid nitrogen and is sublimated under vacuum for 2-3 days. This results in a pure silk powder that can be stored indefinitely. This silk powder can also be resuspended in solvents such as hexafluoroisopropanol (HFIP) in preparation for spinning into a fiber.</p><br />
<br />
<p><b>Material - Fibers</b></p><br />
Silk fibers are the most traditional form. Fibers can be formed from silk that has gone through the lyophilization process and has been resuspended in HFIP. From resuspension, the solution is ‘spun’ into fibers, as detailed below. Silk fibers as a biomedical device are intended to be used as the structural material for scaffolds to assist with re-engineered tissues. Silk scaffolds have the ability to be integrated into bones, cartilage, vascular tissue, and even skin <sup><a href="#references">[3]</a></sup>.<br />
<br />
<p><br/><br/></p><br />
<h2 id="spinning_silk">Spinning Silk</h2><br />
<p>In order to form fibers from silk, soluble silk protein solutions must be much like how they are in natural spider spinnerets. The majority of spinning methods entail pushing, or extruding, silk solution through very thin channels. During this extrusion, shear forces on the silk solution cause the amino acids of the proteins to align in a way that allows the strong beta sheets of the silk structure to form. Multiple proteins are similarly aligned, causing separate proteins to interact and form larger structures.</p><br />
<br />
<p><b>Syringe Extrusion</b></p><br />
<p>The standard method that we are using to produce silk fibers is syringe extrusion, in which a syringe pump forces silk dope through a small-diameter tube into a liquid isopropanol coagulation bath<sup><a href="#references">[1]</a></sup>. This method most directly emulates the process that spiders use. In a natural spider spinneret, silk solution produced in the spider's glands are forced through small-diameter spinnerets, and expelled as solid threads.</p><br />
<br />
<figure style= "margin: 10px; float: right;" align="middle"><a href = "https://static.igem.org/mediawiki/2014/1/1c/Syringe_extruder.JPG"><img src= "https://static.igem.org/mediawiki/2014/1/1c/Syringe_extruder.JPG" width = 25% height = 25% /></a><figcaption style="margin: auto;">A simple syringe extruder setup. It consists of a gas chromatography syringe (Hamilton Co.) in a syringe pump (Pump Systems, Inc.)</figcaption></figure><br />
<br />
<p><b>Centrifuge Extrusion</b></p><br />
<p>Another method that we are investigating is centrifuge extrusion, in which a column with a small-diameter channel is loaded with silk dope, and placed into a centrifuge tube containing the coagulation solution. The loaded column and tube are then centrifuged at high speeds, and the resulting centrifugal force extrudes the dope through the channel into the coagulation bath, causing a fiber to form. The extrusion column was designed this past summer by the UCLA iGEM team for rapid, small scale production of experimental fibers.</p><br />
<br />
<figure style= "margin: 10px; float: right;" align="middle"><a href ="https://static.igem.org/mediawiki/2014/1/1b/Extrusion_column.JPG"><img src= "https://static.igem.org/mediawiki/2014/1/1b/Extrusion_column.JPG" width = 25% height = 25% /></a><figcaption style="margin: auto;">An "extrusion column." 3D printed with a Stratasys PolyJet printer.</figcaption></figure><br />
<br />
<br></br><br />
<br />
<figure style= "margin: 10px; float: right;" align="middle"><br />
<a href ="https://static.igem.org/mediawiki/2014/1/14/Extrusion_column_in_falcon.JPG"><br />
<img src= "https://static.igem.org/mediawiki/2014/1/14/Extrusion_column_in_falcon.JPG" width = 25% height = 25% /><br />
</a><br />
<figcaption style="margin: auto;">Our extrusion column fits into a 15 mL centrifuge tube, which can be kept empty or hold a coagulation bath.</figcaption></figure><br />
<br />
<p><b>Rotary Jet Spinning</b></p><br />
<figure style= "margin: 10px; float: right;" align="middle"><br />
<a href ="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2010/nalefd.2010.10.issue-6/nl101355x/production/images/medium/nl-2010-01355x_0005.gif"><br />
<img src= "https://static.igem.org/mediawiki/2014/7/77/RJS.gif" width = 25% height = 25% /><br />
</a><br />
<figcaption style="margin: auto;">The rotary jet spinner, designed and diagrammed by the Parker lab at MIT<sup><a href="#references">[4]</a></sup>.</figcaption></figure><br />
<br />
<p>A final method for fiber production that we worked with is rotary jet spinning. Rotary jet spinning is very similar to centrifuge extrusion, in that the dope is spun at very high speeds and centrifugal force pushes the dope out of the channel. However, in rotary jet spinning, the dope is loaded into a reservoir that is mounted vertically onto a motor shaft. This method does not allow for the usage of a coagulation bath, as the reservoir holding the dope must be spun in air<sup><a href="#references">[4]</a></sup>. If the reservoir were to be spun in a liquid bath, the resultant turbulence in the bath would cause structural instability and destroy the apparatus.</p><br />
<br />
<br />
<br />
<br />
</div><br />
<br />
<div class= "content_subsection" id="post_spin_processing"><br />
<h2>Post-Spin Stretch</h2><br />
<p>A very important part of creating fibers from expressed recombinant silk is the stretching of the fiber after it has been extruded. This can be observed in actual spiders, who tug on the silk threads they produce in order to stretch them. This stretching not only results in longer lengths of threads to work with, it confers extra strength and elasticity to the fibers <sup><a href="#references">[5]</a></sup>.<br />
</p><br />
<br />
</div><br />
<br />
<div class= "content_subsection" id="accomplishments"><br />
<h2>What We've Done</h2><br />
<p>Over the summer, we carried out steps 1 through 4 of the silk processing procedure according to published protocols (see above). Additionally we made silk pH gels by treating the dialyzed silk with acid. Overnight incubation caused this mixture to solidify into a gel. <br />
We have put together our syringe pump extrusion setup based on published protocols. As an alternative, we came up with idea of extruding our silk via centrifugation. We designed and 3d printed a column with a small opening at the bottom that could hold liquid and extrude it when placed in a falcon tube in the centrifuge.<br />
Although we have not yet attempted to spin a silk fiber, we have spun fibers from solubilized cellulose acetate using both our syringe pump setup as well as our centrifuge extrusion set up. We have lyophilized our dialyzed silk product, and are ready to resuspend this in HFIP solvent and spin it. As soon as we have mastered either the syringe or centrifuge extrusion techniques, we will be ready to produce a fiber from our <i>Bombyx mori</i> silk proteins.<br />
<br />
</p><br />
<br />
</div><br />
<br />
<br />
<div class = "content_subsection"><br />
<h2>References</h2><br />
<br />
<p><sup>[1]</sup>Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." <i>Nature Protocols</i> 4.3 (2009): 341-55. Web.</p><br />
<br />
<p><sup>[2]</sup>Kim, Dae-Hyeong, Jonathan Viventi, Jason J. Amsden, Jianliang Xiao, Leif Vigeland, Yun-Soung Kim, Justin A. Blanco, Bruce Panilaitis, Eric S. Frechette, Diego Contreras, David L. Kaplan, Fiorenzo G. Omenetto, Yonggang Huang, Keh-Chih Hwang, Mitchell R. Zakin, Brian Litt, and John A. Rogers. "Dissolvable Films of Silk Fibroin for Ultrathin Conformal Bio-integrated Electronics." <i>Nature Materials</i> 9.6 (2010): 511-17. Web.</p><br />
<br />
<p><sup>[3]</sup> Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." <i>Nature Protocols</i> 6.10 (2011): 1612-631. Web.</p><br />
<br />
<br />
<p><sup>[4]</sup>Badrossamay, Mohammad Reza, Holly Alice Mcilwee, Josue A. Goss, and Kevin Kit Parker. "Nanofiber Assembly by Rotary Jet-Spinning." <i>Nano Letters</i> 10.6 (2010): 2257-261. Web.</p><br />
<br />
<p><sup>[5]</sup>An, Bo, Michael B. Hinman, Gregory P. Holland, Jeffery L. Yarger, and Randolph V. Lewis. "Inducing β-Sheets Formation in Synthetic Spider Silk Fibers by Aqueous Post-Spin Stretching." <i>Biomacromolecules</i 12.6 (2011): 2375-381. Web.</p><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T03:24:47Z<p>Michaelc1618: </p>
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<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<br></br><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<DD>We contemplated the responsibility that synthetic biologists have regarding the ethics of not only their own work, but their colleagues' work as well. Find our conclusion <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">here on our Human Practices page</a>.<br />
</DL><br />
<br />
<h3>Gold Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Improve the function OR characterization of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part.</DT><br />
<DD>We will be characterizing a few Utah 2012 iGEM Teams in the upcoming weeks to compare them with our own constructs.</DD><br />
<br />
<DT>&#10003; Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</DT><br />
<DD>In collaboration with the Imperial iGEM team, we worked to revive the iGEM subreddit as an online forum for discussion and collaboration amongst iGEM teams.</DD><br />
<DD>Additionally, although they are not yet a registered iGEM team, we provided the University of LaVerne with advice and logistical support on how to begin their own iGEM team.</DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team's scientific project, and how it might be adapted for others to use (within and beyond iGEM). We encourage thoughtful and creative approaches, and those that draw on past Policy & Practice (formerly Human Practices) activities.</DT><br />
<DD>Please refer to our <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">Human Practices</a> page to learn more about our involvement with ethics, safety, and security.</DD><br />
<br />
</DL><br />
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</div><br />
</div><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T03:24:19Z<p>Michaelc1618: </p>
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<div class= "page_content" id= "section1"><br />
<div class= "content_subsection" id="judging"><br />
<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<br></br><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<DD>We contemplated the responsibility that synthetic biologists have regarding the ethics of not only their own work, but their colleagues' work as well. Find our conclusion <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">here on our Human Practices page</a>.<br />
</DL><br />
<br />
<h3>Gold Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Improve the function OR characterization of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part.</DT><br />
<DD>We will be characterizing a few Utah 2012 iGEM Teams in the upcoming weeks to compare them with our own constructs.</DD><br />
<br />
<DT>&#10003; Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</DT><br />
<DD>In collaboration with the Imperial iGEM team, we worked to revive the <a href="www.reddit.com/r/igem">iGEM subreddit</a> as an online forum for discussion and collaboration amongst iGEM teams.</DD><br />
<DD>Additionally, although they are not yet a registered iGEM team, we provided the University of LaVerne with advice and logistical support on how to begin their own iGEM team.</DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team's scientific project, and how it might be adapted for others to use (within and beyond iGEM). We encourage thoughtful and creative approaches, and those that draw on past Policy & Practice (formerly Human Practices) activities.</DT><br />
<DD>Please refer to our <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">Human Practices</a> page to learn more about our involvement with ethics, safety, and security.</DD><br />
<br />
</DL><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T03:23:31Z<p>Michaelc1618: </p>
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<!--CONTENT--><br />
<div class= "content_container" style="top:200px"><br />
<div class= "page_content" id= "section1"><br />
<div class= "content_subsection" id="judging"><br />
<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<br></br><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<DD>We contemplated the responsibility that synthetic biologists have regarding the ethics of not only their own work, but their colleagues' work as well. Find our conclusion <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">here on our Human Practices page</a>.<br />
</DL><br />
<br />
<h3>Gold Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Improve the function OR characterization of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part.</DT><br />
<DD>We will be characterizing a few Utah 2012 iGEM Teams in the upcoming weeks to compare them with our own constructs.</DD><br />
<br />
<DT>&#10003; Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</DT><br />
<DD>In collaboration with the Imperial iGEM team, we worked to revive the <a href="reddit.com/r/igem">iGEM subreddit</a> as an online forum for discussion and collaboration amongst iGEM teams.</DD><br />
<DD>Additionally, although they are not yet a registered iGEM team, we provided the University of LaVerne with advice and logistical support on how to begin their own iGEM team.</DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team's scientific project, and how it might be adapted for others to use (within and beyond iGEM). We encourage thoughtful and creative approaches, and those that draw on past Policy & Practice (formerly Human Practices) activities.</DT><br />
<DD>Please refer to our <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">Human Practices</a> page to learn more about our involvement with ethics, safety, and security.</DD><br />
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<h1>Judging</h1><br />
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<h3>Bronze Medal Requirements</h3><br />
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<DT>&#10004; Register the team and have a great summer.</DT><br />
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<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
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<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
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<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
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<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
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<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
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<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
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<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
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<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<DD>We contemplated the responsibility that synthetic biologists have regarding the ethics of not only their own work, but their colleagues' work as well. Find our conclusion <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">here on our Human Practices page</a>.<br />
</DL><br />
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<h3>Gold Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Improve the function OR characterization of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part.</DT><br />
<DD>We will be characterizing a few Utah 2012 iGEM Teams in the upcoming weeks to compare them with our own constructs.</DD><br />
<br />
<DT>&#10063; Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.</DT><br />
<DD>In collaboration with the Imperial iGEM team, we worked to revive the <a href="reddit.com/r/igem">iGEM subreddit</a> as an online forum for discussion and collaboration amongst iGEM teams.</DD><br />
<DD>Additionally, although they are not yet a registered iGEM team, we provided the University of LaVerne with advice and logistical support on how to begin their own iGEM team.</DD><br />
<br />
<DT>&#10003; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team's scientific project, and how it might be adapted for others to use (within and beyond iGEM). We encourage thoughtful and creative approaches, and those that draw on past Policy & Practice (formerly Human Practices) activities.</DT><br />
<DD>Please refer to our <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">Human Practices</a> page to learn more about our involvement with ethics, safety, and security.</DD><br />
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
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<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community. In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
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<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Art | Sci Presents Seres: Inhabitants of the Land of Silk</a><br/><br />
<a href="http://micklorusso.net/patterns%20that%20connect/Mick%20Lorusso.html">Mick Lorusso, curator of the exhibit</a><br/><br />
<a href="http://victoriavesna.com/index.php">Victoria Vesna, director of UCLA Art | Sci</a><br/><br />
<a href="http://www.diysect.com/">DIYSECT: A Documentary Web-Series on DIY Biology and Bioart</a><br/><br />
<a href="http://marytsang.com/">Mary Tsang, director of DIYSECT</a><br/><br />
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<h2>Acknowledgements</h2><br />
<p><strong><i>Advising:</i></strong><br /><br />
Dr. Sriram Kosuri<br /><br />
Dr. David Breslauer<br /><br />
Dr. Mark Arbing<br /><br />
Keegan Owsley<br /><br />
David Yao<br /><br/><br />
<br />
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<strong><i>Lab Space:</i></strong><br /><br />
Dr. Foad Mashayekhi<br /><br />
Dr. Dino DiCarlo<br /><br />
Garrett Mosley<br /><br />
UCLA Department of Bioengineering<br /><br /><br />
<br />
<strong><i>Financial Support:</i></strong><br /><br />
UCLA Department of Bioengineering<br /><br />
UCLA Department of Chemistry and Biochemistry<br /><br />
UCLA Department of Microbiology, Immunology, and Molecular Genetics<br /><br />
Refactored Materials<br /><br />
Office of the Dean of Undergraduate Education<br /><br />
Tama Hasson, Vice Provost for Undergraduate Research<br /><br /><br />
<br />
<strong><i>Art | Sci Collaboration:</i></strong><br /><br />
Mick Lorusso<br /><br />
Dr. Victoria Vesna<br /><br /><br />
<br />
<strong><i>Administrative Support:</i></strong><br /><br />
Stacey Tran<br /><br />
Brian Lee<br /><br /><br />
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<h2>Acknowledgements</h2><br />
<p><strong><i>Advising:</i></strong><br /><br />
Dr. Sriram Kosuri<br /><br />
Dr. David Breslauer<br /><br />
Dr. Mark Arbing<br /><br />
Keegan Owsley<br /><br />
David Yao<br /><br/><br />
<br />
<br />
<strong><i>Lab Space:</i></strong><br /><br />
Dr. Foad Mashayekhi<br /><br />
Dr. Dino DiCarlo<br /><br />
Garrett Mosley<br /><br />
UCLA Department of Bioengineering<br /><br /><br />
<br />
<strong><i>Financial Support:</i></strong><br /><br />
UCLA Department of Bioengineering<br /><br />
UCLA Department of Chemistry and Biochemistry<br /><br />
UCLA Department of Microbiology, Immunology, and Molecular Genetics<br /><br />
Refactored Materials<br /><br />
Office of the Dean of Undergraduate Education<br /><br />
Tama Hasson, Vice Provost for Undergraduate Research<br /><br /><br />
<br />
<strong><i>Art | Sci Collaboration:</i></strong><br /><br />
Mick Lorusso<br /><br />
Dr. Victoria Vesna<br /><br /><br />
<br />
<strong><i>Administrative Support:</i></strong><br /><br />
Stacey Tran<br /><br /><br />
<br />
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<p><strong><i>Advising:</i></strong><br /><br />
Dr. Sriram Kosuri<br /><br />
Dr. David Breslauer<br /><br />
Dr. Mark Arbing<br /><br />
Keegan Owsley<br /><br />
David Yao<br /><br/><br />
<br />
<br />
<strong><i>Lab Space:</i></strong><br /><br />
Dr. Foad Mashayekhi<br /><br />
Dr. Dino DiCarlo<br /><br />
Garrett Mosley<br /><br />
UCLA Department of Bioengineering<br /><br /><br />
<br />
<strong><i>Financial Support:</i></strong><br /><br />
UCLA Department of Bioengineering<br /><br />
UCLA Department of Chemistry and Biochemistry<br /><br />
UCLA Department of Microbiology, Immunology, and Molecular Genetics<br /><br />
Refactored Materials<br /><br />
Office of the Dean of Undergraduate Education<br /><br />
Tama Hasson, Vice Provost for Undergraduate Research<br /><br /><br />
<br />
<strong><i>Art | Sci Collaboration:</i></strong><br /><br />
Mick Lorusso<br /><br />
Dr. Victoria Vesna<br /><br />
<strong><i>Administrative Support:</i></strong><br /><br />
Stacey Tran<br /><br /><br />
<br />
<br />
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<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<br></br><br />
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<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<DD>We contemplated the responsibility that synthetic biologists have regarding the ethics of not only their own work, but their colleagues' work as well. Find our conclusion <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">here on our Human Practices page</a>.<br />
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<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
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</DL><br />
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<br></br><br />
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<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<DD>Look at our answer <a href="https://2014.igem.org/Team:UCLA/Project/Human_Practices">here on our Human Practices page</a>.<br />
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<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<br></br><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10004; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Project/Human_PracticesTeam:UCLA/Project/Human Practices2014-10-18T02:45:10Z<p>Michaelc1618: </p>
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
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<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community. In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
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<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Art | Sci Presents Seres: Inhabitants of the Land of Silk</a><br/><br />
<a href="http://micklorusso.net/patterns%20that%20connect/Mick%20Lorusso.html">Mick Lorusso, curator of the exhibit</a><br/><br />
<a href="http://www.diysect.com/">DIYSECT: A Documentary Web-Series on DIY Biology and Bioart</a><br/><br />
<a href="http://marytsang.com/">Mary Tsang, director of DIYSECT</a><br/><br />
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
</div><br />
<br />
<div class= "content_subsection" id="laser"><br />
<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community. In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
<br />
<p><br/></p><br />
</div><br />
<br />
<div class= "content_subsection" id="more_info"><br />
<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Art|Sci Presents Seres: Inhabitants of the Land of Silk</a><br/><br />
<a href="http://artsci.ucla.edu/sites/artsci.ucla.edu/files/SilkUCLAArtSciGalleryOct2nd.pdf">Link 2</a><br/><br />
<a href="http://www.diysect.com/">DIYSECT: A Documentary Web-Series on DIY Biology and Bioart</a><br/><br />
<a href="http://marytsang.com/">Mary Tsang, director of DIYSECT</a><br/><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Project/Human_PracticesTeam:UCLA/Project/Human Practices2014-10-18T02:39:34Z<p>Michaelc1618: </p>
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
</div><br />
<br />
<div class= "content_subsection" id="laser"><br />
<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community. In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
<br />
<p><br/></p><br />
</div><br />
<br />
<div class= "content_subsection" id="more_info"><br />
<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Art|Sci Presents Seres: Inhabitants of the Land of Silk</a><br/><br />
<a href="http://artsci.ucla.edu/sites/artsci.ucla.edu/files/SilkUCLAArtSciGalleryOct2nd.pdf">Link 2</a><br/><br />
<a href="http://www.diysect.com/">DIYSECT: A Documentary Web-Series on DIY Biology and Bioart</a><br/><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
<br />
<br />
<!--END CONTENT--><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Project/Human_PracticesTeam:UCLA/Project/Human Practices2014-10-18T02:36:26Z<p>Michaelc1618: </p>
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
</div><br />
<br />
<div class= "content_subsection" id="laser"><br />
<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community. In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
<br />
<p><br/></p><br />
</div><br />
<br />
<div class= "content_subsection" id="more_info"><br />
<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Art|Sci Presents: Inhabitants in the Land of Silk</a><br/><br />
<a href="http://artsci.ucla.edu/sites/artsci.ucla.edu/files/SilkUCLAArtSciGalleryOct2nd.pdf">Link 2</a><br/><br />
<a href="http://www.diysect.com/">Link 3</a><br/><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Project/Human_PracticesTeam:UCLA/Project/Human Practices2014-10-18T02:34:54Z<p>Michaelc1618: </p>
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
</div><br />
<br />
<div class= "content_subsection" id="laser"><br />
<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community. In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
<br />
<p><br/><br/></p><br />
</div><br />
<br />
<div class= "content_subsection" id="more_info"><br />
<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Link 1</a><br/><br />
<a href="http://artsci.ucla.edu/sites/artsci.ucla.edu/files/SilkUCLAArtSciGalleryOct2nd.pdf">Link 2</a><br/><br />
<a href="http://www.diysect.com/">Link 3</a><br/><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Project/Human_PracticesTeam:UCLA/Project/Human Practices2014-10-18T02:34:23Z<p>Michaelc1618: </p>
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<h1>Human Practices</h1><br />
<h2>Seres: Inhabitants of the Land of Silk</h2><br />
<p>From our first contact with processing silk, our group became enamored with the look and feel of our silk, and the different forms it could take. We began to get involved with not just the scientific value, but the aesthetic of our silk products, and we knew there must be some application to it outside our laboratory.</p><br />
<p><br />
Also from the onset of our project, we knew we wanted to share our fascination with silk to the outside community, and explore what potential uses our project could have. We dreamed big: we wanted our project to reach world of biomedical devices, optics and fashion. But we were also interested in what potential others saw in our project, and after reaching out to the community, our human practices was born.</p><br />
<p><br />
Titled "Seres: Inhabitants of the Land of Silk," our project is a collaboration with the UCLA Art | Sci Center dedicated to bridge the gap between art and science through an open exhibition. On display is work from artist Jason Fahrion, who grows silkworms and creates mosaics out of their naturally fluorescent coccoons, as well as our own iGEM wall, where we lay out an interactive web-like diagram breaking down how we process our silk into different materials like fibers, gels and dust, with samples for guests to see and feel and experience the silk the same way we get to in our lab. Also along the walls are other forms of silk art, including a glass display of live silkworms feeding on mulberry leaves, Jason's mosaics and a display of the historical uses of silkworms and their silk. Through this, we hope to have the community explore with us the art and beauty behind our science, and the science behind what we see as art. </p><br />
<p><br />
The exhibition is open to the pubic, and here is a look inside <a href="http://dailybruin.com/2014/10/02/exhibition-weaves-art-science-together-with-silk/">"Seres: Inhabitants of the Land of Silk."</a></p><br />
</div><br />
<br />
<div class= "content_subsection" id="laser"><br />
<h2>LASER (Leonardo Art Science Evening Rendezvous):</h2><br />
<p>Following the exhibition was a series of talks, the Leonardo Art Science Evening Rendezvous (LASER). This talk featured a number of artists who explore the relationship between science and art. Among them was filmmaker Mary Tsang, who recently released DIYSECT, a documentary on DIY Biology and the influence that it had on the scientific and artistic community (DIYSECT). In her talk, she mentioned that a number of community Biology laboratories had been contacted by the Federal Bureau of Investigation regarding their responsibility to remain vigilant of the possibility of individuals misusing synthetic biology to cause harm and damage. </p><br />
<br />
<p>This was a stark reminder that while all of us participating in iGEM seek to use synthetic biology to create beneficial technologies, it does have the potential to raise threats and cause serious harm. As a team, we discussed this in depth over the next few weeks and came to an agreement that as synthetic biologists, we do have a responsibility to be aware of what happens in our field. We felt that there is no need to treat our fellow synthetic biologists with suspicion, as the tone of the FBI appeared to recommend. However, we agreed with the fact that we have an obligation to not only conduct our own projects and experiments ethically, but to ensure that others in our field do so as well. </p><br />
<br />
<p><br/><br/></p><br />
</div><br />
<br />
<div class= "content_subsection" id="more_info"><br />
<h2>Learn More: </h2><br />
<p>To learn more about our collaboration with the UCLA Art | Sci Center, as well as the other speakers who attended the LASER talk session, check out the following links:</p><br />
<a href="http://www.ucira.ucsb.edu/ucla-artsci-gallery-presents-seres-inhabitants-of-the-land-of-silk/">Link 1</a><br/><br />
<a href="http://artsci.ucla.edu/sites/artsci.ucla.edu/files/SilkUCLAArtSciGalleryOct2nd.pdf">Link 2</a><br/><br />
<a href="http://www.diysect.com/">Link 3</a><br/><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:22:46Z<p>Michaelc1618: </p>
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<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
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<br></br><br />
<br></br><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
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<!--<ul style="list-style-type:none;">--><br />
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<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:20:34Z<p>Michaelc1618: </p>
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<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<br></br><br />
<br></br><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
</DL><br />
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<br></br><br />
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<h3>Gold Medal Requirements</h3><br />
<DL><br />
<br />
<br />
<br />
</DL><br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
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<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:16:34Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
</DL><br />
<br />
<br />
<h3>Gold Medal Requirements</h3><br />
<DL><br />
<br />
<br />
<br />
</DL><br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:15:24Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>&#10063; iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<br />
<br />
</DL><br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:15:02Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>&#10063; Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<br />
<br />
</DL><br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:14:43Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
</DL><br />
<br />
<h3>Silver Medal Requirements</h3><br />
<DL><br />
<br />
<DT>Experimentally validate that at least one new BioBrick part or device of your own design and construction works as expected.</DT><br />
<DD>&#10063; We will be validating our BioBrick soon! Stay tuned!</DD><br />
<br />
<DT>&#10063; Document the characterization of this part in the Main Page section of that Part's/Device's Registry entry.</DT><br />
<DD>We will update our part's documentation as soon as possible.</DD><br />
<br />
<DT>&#10004; Submit this new part to the iGEM Parts Registry.</DT><br />
<DD>We have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DD><br />
<br />
<DT>iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, or intellectual property rights. Articulate at least one question encountered by your team, and describe how your team considered the(se) question(s) within your project. Include attributions to all experts and stakeholders consulted.</DT><br />
<br />
<br />
</DL><br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:10:03Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#10004; Register the team and have a great summer.</DT><br />
<br />
<DT>&#10004; Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>&#10004; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>&#10004; Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>&#10004; Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>&#10004; Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DT><br />
<br />
</DL><br />
<br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:09:23Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#9633; Register the team and have a great summer.</DT><br />
<br />
<DT>Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<DT>Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</DT><br />
<br />
<DT>Plan to present a poster and talk at the iGEM Jamboree.</DT><br />
<br />
<DT>Distinguish work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.</DT><br />
<DD>All attributions can be found on our <a href="https://2014.igem.org/Team:UCLA">wiki</a>.<br />
<br />
<DT>Document at least one new standard BioBrick part or device used in your project and submit this part to the iGEM Registry.</DT><br />
<DD>Thus far, we have submitted <a href="http://parts.igem.org/Part:BBa_K1384000">BBa_K1384000</a></DT><br />
<br />
</DL><br />
<br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:06:13Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#9633; Register the team and have a great summer.</DT><br />
<br />
<DT>Successfully complete and submit the iGEM 2014 Judging Form.</DT><br />
<DD>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></DD><br />
<br />
<br />
</DL><br />
<br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:05:26Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#9633; Register the team and have a great summer.</DT><br />
<DD>We certainly had a great summer.</DD><br />
<br />
<br />
</DL><br />
<br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:05:06Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<h3>Bronze Medal Requirements</h3><br />
<DL><br />
<DT>&#9633; Register the team and have a great summer.</DT><br />
<br />
<br />
</DL><br />
<br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:04:40Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<DL><br />
<DT>&#9633; Register the team and have a great summer.</DT><br />
<br />
<br />
</DL><br />
<br />
<br />
<br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:04:23Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<DL><br />
<DT>&#9633; Register the team and have a great summer.</DT><br />
<br />
<br />
</DL><br />
<!--<ul style="list-style-type:none;">--><br />
<br />
<!--<br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ulJ><br />
--><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:01:57Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ulN><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Template/CSSTeam:UCLA/Template/CSS2014-10-18T01:01:47Z<p>Michaelc1618: </p>
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:01:04Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer.</li><br />
<li>&#9633; Successfully complete and submit the iGEM 2014 Judging form.</li><br />
<ul><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
<br />
<li>&#9633; Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:00:10Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer</li><br />
<li>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</li><br />
<ul><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T01:00:03Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer</li><br />
<li>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</li><br />
<ulJ><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:59:47Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer</li><br />
<li>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</li><br />
<ul><li>Our judging form can be found <a href="https://igem.org/2014_Judging_Form?id=1384">here</a></li></ul><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:58:00Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ulJ><br />
<li>&#9633; Register the team and have a great summer</li><br />
<p>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</p><br />
<p><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Template/CSSTeam:UCLA/Template/CSS2014-10-18T00:57:37Z<p>Michaelc1618: </p>
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#menu a:hover{<br />
background:#0099CC;<br />
}<br />
#menu li ul{<br />
background:#006699;<br />
/* display:none; */<br />
position:absolute;<br />
text-align:center;<br />
top:50px;<br />
right:0px;<br />
}<br />
#menu li:hover ul{<br />
display:block;<br />
}<br />
#dropdown_menu li ul li{<br />
position:relative;<br />
}<br />
</style><br />
<br />
<br />
<br />
<!--SLIDER--><br />
<style type="text/css"><br />
.slider{<br />
position: relative;<br />
width: 1150px;<br />
height: 350px;<br />
margin: auto;<br />
overflow: hidden;<br />
z-index: 13;<br />
background-image: url('https://static.igem.org/mediawiki/2014/c/c3/Debut_light.png');<br />
}<br />
.slider .slider_left{<br />
position: absolute;<br />
background-color:black;<br />
top: 0px; <br />
left: 0px;<br />
width: 50px;<br />
height: 100%;<br />
opacity: 0.5;<br />
z-index: 2;<br />
}<br />
.slider .slider_left img{<br />
position: relative;<br />
width: 100%;<br />
height: 200px;<br />
top: 25%;<br />
left: 10px;<br />
}<br />
.slider .slider_right{<br />
position: absolute;<br />
background-color:black;<br />
top: 0px;<br />
left: 1100px;<br />
width: 50px;<br />
height: 100%;<br />
opacity: 0.5;<br />
z-index: 2;<br />
}<br />
.slider .slider_right img{<br />
position: relative;<br />
width: 100%;<br />
height: 200px;<br />
top: 25%;<br />
left: -10px;<br />
}<br />
.slider .slider_imgs{<br />
position: absolute;<br />
}<br />
.slider .slider_imgs div{<br />
float: left;<br />
width: 1150px;<br />
height: 350px;<br />
}<br />
.slider .slider_imgs #slide1{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/d/d1/Silkworms.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 25% 50%;<br />
}<br />
.slider .slider_imgs #slide2{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/8/81/Classroom.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 50%;<br />
}<br />
.slider .slider_imgs #slide3{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fa/Cocoons.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
}<br />
.slider .slider_imgs #slide4{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fd/Uclateam.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
</style><br />
<br />
<br />
<br />
<!--HP SLIDER--><br />
<style type="text/css"><br />
.hpslider{<br />
position: relative;<br />
width: 1150px;<br />
height: 350px;<br />
margin: auto;<br />
overflow: hidden;<br />
z-index: 13;<br />
background-image: url('https://static.igem.org/mediawiki/2014/c/c3/Debut_light.png');<br />
}<br />
.hpslider .hpslider_left{<br />
position: absolute;<br />
background-color:black;<br />
top: 0px; <br />
left: 0px;<br />
width: 50px;<br />
height: 100%;<br />
opacity: 0.5;<br />
z-index: 2;<br />
}<br />
.hpslider .hpslider_left img{<br />
position: relative;<br />
width: 100%;<br />
height: 200px;<br />
top: 25%;<br />
left: 10px;<br />
}<br />
.hpslider .hpslider_right{<br />
position: absolute;<br />
background-color:black;<br />
top: 0px;<br />
left: 1100px;<br />
width: 50px;<br />
height: 100%;<br />
opacity: 0.5;<br />
z-index: 2;<br />
}<br />
.hpslider .hpslider_right img{<br />
position: relative;<br />
width: 100%;<br />
height: 200px;<br />
top: 25%;<br />
left: -10px;<br />
}<br />
.hpslider .hpslider_imgs{<br />
position: absolute;<br />
}<br />
.hpslider .hpslider_imgs div{<br />
float: left;<br />
width: 1150px;<br />
height: 350px;<br />
}<br />
.hpslider .hpslider_imgs #slide1{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/d/d1/Silkworms.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 25% 50%;<br />
}<br />
.hpslider .hpslider_imgs #slide2{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/8/81/Classroom.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 50%;<br />
}<br />
.hpslider .hpslider_imgs #slide3{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fa/Cocoons.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
}<br />
.hpslider .hpslider_imgs #slide4{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fd/Uclateam.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide5{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/2/28/Fahrion_UCLA_iGEM_statement.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide6{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/9/96/Fahrion_Non_Fluoresce.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide7{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/b/b3/Fahrion_Blue_Fluoresce_side.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide8{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/4/4c/Fahrion_process_Anuved_Dewal.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide9{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/9/95/Fahrion_Process_full.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide10{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/f4/Fahrion_full_nonfluoresce.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide11{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/5/58/Fahrion_Group.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide12{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/b/be/Fahrion_David_Discussion.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide13{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/5/59/Fahrion_Process_Aal.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide14{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fa/Cocoons.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide15{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/e/ef/Fahrion_Blue_Straight.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide16{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/6/62/Fahrion_Process_Observers.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide17{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fd/Uclateam.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
.hpslider .hpslider_imgs #slide18{<br />
float: left;<br />
background-image:url('https://static.igem.org/mediawiki/2014/5/57/Fahrion_Historical_Wall.JPG');<br />
background-size: cover;<br />
background-repeat: no-repeat;<br />
background-position: 0% 65%;<br />
}<br />
</style><br />
<br />
<br />
<br />
<br />
<!--PAGE HEADERS--><br />
<style type="text/css"><br />
.page_header{<br />
position: absolute;<br />
background-image: url('https://static.igem.org/mediawiki/2014/b/b3/Debut_dark.png');<br />
width: 100%;<br />
height: 70%;<br />
z-index: 0;<br />
margin-bottom: 200px;<br />
}<br />
.page_header .page_header_box{<br />
position: absolute;<br />
overflow: hidden;<br />
z-index: 0;<br />
}<br />
#box1{<br />
background-image:url('https://static.igem.org/mediawiki/2014/f/fa/Cocoons.JPG');<br />
background-size:1500px;<br />
width:21%;<br />
height:80%;<br />
top:10%;<br />
left:10%;<br />
opacity: 0.5;<br />
}<br />
#box2{<br />
background-image:url('http://www.visualphotos.com/photo/1x7581974/silk_fibres_coloured_sem_H1200410.jpg');<br />
background-position:50% 50%;<br />
width:21%;<br />
height:80%;<br />
top:10%;<br />
left:30%;<br />
opacity: 0.5;<br />
}<br />
#box3{<br />
background-image: url('https://static.igem.org/mediawiki/2014/3/31/Pcrgel.jpg');<br />
background-size:750px;<br />
background-position:70% 70%;<br />
width:21%;<br />
height:80%;<br />
top:10%;<br />
left:50%;<br />
opacity: 0.5;<br />
}<br />
#box4{<br />
background-image:url('https://static.igem.org/mediawiki/2014/a/a2/Spiderweb.jpg');<br />
<br />
width:21%;<br />
height:80%;<br />
top:10%;<br />
left:70%;<br />
opacity: 0.5;<br />
}<br />
</style><br />
<br />
<br />
<br />
<!--CONTENT SECTIONS--><br />
<style type="text/css"><br />
.content_container{<br />
position: absolute;<br />
background-image: url('https://static.igem.org/mediawiki/2014/c/c3/Debut_light.png');<br />
font-family: 'Roboto', sans-serif;<br />
top: 85%;<br />
width: 100%;<br />
padding: 0 0 0 0;<br />
margin: auto;<br />
margin-bottom: 50px;<br />
margin-left: 0px;<br />
border-radius: 0px;<br />
z-index: -1;<br />
}<br />
<br />
.page_content{<br />
position: relative;<br />
background-color: #FFEE80;<br />
width: 99%;<br />
padding: 0 0 0 0;<br />
margin: auto;<br />
margin-bottom: 20px;<br />
/*border-radius: 25px;*/<br />
z-index: -1;<br />
}<br />
.content_subsection{<br />
position: relative;<br />
width: 85%;<br />
padding: 10px 10px 10px 10px;<br />
margin: auto;<br />
}<br />
</style><br />
<br />
<!--STYLING FOR TABLES--><br />
<style><br />
table{<br />
font-family: 'Roboto', sans-serif;<br />
background-image: url('https://static.igem.org/mediawiki/2014/c/cb/Debut_light.png');<br />
}<br />
<br />
ulJ<br />
{<br />
list-style-type: none;<br />
}<br />
<br />
</style><br />
<br />
<br />
<br />
<br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/Template/CSSTeam:UCLA/Template/CSS2014-10-18T00:57:16Z<p>Michaelc1618: </p>
<hr />
<div><html><br />
<link href='http://fonts.googleapis.com/css?family=Pacifico' rel='stylesheet' type='text/css'><br />
<link href='http://fonts.googleapis.com/css?family=Kaushan+Script' rel='stylesheet' type='text/css'><br />
<link href='http://fonts.googleapis.com/css?family=Bad+Script' rel='stylesheet' type='text/css'><br />
<br />
<br />
<!--WIKI TEMPLATE FORMATTING--><br />
<style type="text/css"><br />
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/<br />
<br />
.firstHeading {<br />
width: 975px; <br />
margin: 0px auto;<br />
padding-top: 100px;<br />
margin-bottom: 20px;<br />
font-family: Georgia, Times, "Times New Roman", serif;<br />
display:none;<br />
}<br />
<br />
h1, h2, h3, h4, h5 {<br />
/*font-family: Helvetica, Georgia, Times, "Times New Roman", serif;*/<br />
font-family: 'Roboto', sans-serif;<br />
border-bottom: 0px solid #aaa;<br />
}<br />
<br />
<br />
#centercontainer { margin: auto; text-align: center; width: 100%}/* CENTERING CONTAINER: Used for centering three content boxes */<br />
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/<br />
background-color: #383838;<br />
border: 0 none;<br />
height: 14px;<br />
z-index: 100;<br />
top: 0;<br />
position: fixed;<br />
width: 975px; <br />
left: 50%; <br />
margin-left: -487px;<br />
}<br />
<br />
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/<br />
background-color: #383838;<br />
height: 14px;<br />
display: block;<br />
z-index: 10;<br />
position: fixed;<br />
width: 100%;<br />
top: 0;<br />
}<br />
<br />
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/<br />
color: #727272; <br />
text-decoration: none; <br />
background-color: transparent;<br />
}<br />
<br />
body { <br />
background-color: #fff;<br />
}<br />
<br />
#globalWrapper, #content { /*-- changes default wiki settings --*/<br />
width: 100%; <br />
height: 100%;<br />
border: 0px;<br />
background-color: transparent;<br />
margin: 0px;<br />
padding: 0px;<br />
}<br />
<br />
html, body, .wrapper { /*-- changes default wiki settings --*/<br />
width: 100%; <br />
height: 100%; <br />
background-image: url('https://static.igem.org/mediawiki/2014/c/cb/Debut_light.png');<br />
/*background-size: cover;*/<br />
}<br />
<br />
<br />
<br />
#contentcontainer { /*-- creates container for all content on page --*/<br />
font-family: Arial, Helvetica, sans-serif;<br />
font-family: 'Roboto', sans-serif;<br />
font-weight: normal; <br />
font-size: 14px; <br />
color: #414141;<br />
width: 960px; <br />
margin-left: auto; <br />
margin-right: auto; <br />
background-color: #transparent; <br />
margin-top: 0px; <br />
}<br />
<br />
.sidemenu, .sidemenu li { <br />
list-style-type: none; <br />
list-style-image: none; <br />
font-family: verdana; <br />
text-decoration: none; <br />
color:#000; <br />
font-size: 14px;<br />
}<br />
<br />
.sidemenu li {display: block;}<br />
<br />
.sidemenu a {<br />
text-decoration:none; <br />
color: #000;<br />
display: block; <br />
width: 180px; <br />
height: 100%; <br />
padding: 3px 5px; <br />
transition: .25s ease-in-out;<br />
-moz-transition: .25s ease-in-out;<br />
-webkit-transition: .25s ease-in-out;<br />
} <br />
<br />
.sidemenu a.greyout { color: #909090; }<br />
<br />
.sidemenu a:hover {<br />
color:#666; <br />
background-color: #d9f5aa;<br />
}<br />
<br />
.sub-sidemenu {font-size: 10px; margin-bottom: 20px; color: #666; display: none;}<br />
.sub-sidemenu a {color: #666;}<br />
<br />
div.underconst<br />
{<br />
padding:10px;<br />
border-radius:5px;<br />
background-color:pink;<br />
text-align:center;<br />
}<br />
<br />
a.anchor{display: block; position: relative; top: -80px; visibility: hidden;}<br />
<br />
.contentpara {margin-bottom: 30px;}<br />
<br />
span.email{font-family: monospace; font-weight: normal;}<br />
<br />
fieldset {border: 1px solid #337f53;}<br />
<br />
.greyout {color: #A0A0A0;}<br />
.highlightme {background-color: #FFFF00;}<br />
<br />
#alertContainer {border: 2px solid red; margin-bottom: 10px; width: 936px;}<br />
#annContainer {margin-left: 8px;}<br />
#newsContainer {border: 1px solid #ccc;}<br />
<br />
.newsTitle {<br />
display: block; <br />
color: #414141;<br />
font-size: 25px; <br />
/*font-family: Georgia, Times, "Times New Roman", serif;*/<br />
font-family: 'Roboto', sans-serif;<br />
padding: 10px 15px 5px 10px; <br />
border-bottom: 1px solid #ccc;<br />
margin-bottom: 0px; <br />
}<br />
<br />
.newsItem {<br />
border-bottom: 1px solid #ccc; <br />
display: block; <br />
padding: 5px 15px 0px 10px; <br />
margin-bottom: 0px; <br />
}<br />
<br />
.newsItem h3 {<br />
width: auto; <br />
display: inline; <br />
font-size: 14px; <br />
font-family: Arial, Helvetica, sans-serif; <br />
padding: 0px; <br />
margin: 0px;<br />
}<br />
<br />
.newsItem img {<br />
float: right; <br />
clear: right; <br />
width: 80px; <br />
padding-left: 15px;<br />
display: inline-block; <br />
}<br />
<br />
.newsItem .newsDate {<br />
font-style: italic; <br />
font-size: 14px; <br />
display: inline-block; <br />
color: #999; <br />
float: right; <br />
padding: 0px 0px 10px 15px;<br />
}<br />
<br />
.newsItem p, .newsItem ul, .newsItem li {<br />
padding: 0px; <br />
margin-left: 20px; <br />
font-size: 12px; <br />
line-height: 1.2;<br />
}<br />
<br />
.newsItem p {margin-top: 10px;}<br />
<br />
.annItem {<br />
border: 2px solid #49b677; <br />
display: block; <br />
padding: 0px 15px 10px 10px; <br />
margin-bottom: 18px; <br />
position: relative; <br />
height: 160px; <br />
vertical-align: middle;<br />
}<br />
<br />
.annItem .annCentered {<br />
display: table-cell; <br />
vertical-align: middle; <br />
height: 160px;<br />
}<br />
<br />
.annCentered h3 {<br />
padding: 0px;<br />
display: block; <br />
margin-left: auto; <br />
margin-right: auto; <br />
text-align: center;<br />
}<br />
<br />
.annItem ul, .annItem li {padding: 0px; margin: 0px;}<br />
.annItem li {list-style: none; margin: 5px}<br />
<br />
<!--TEXT STYLING--><br />
hr.style-one {<br />
border: 0;<br />
height: 1px;<br />
background: #333;<br />
background-image: -webkit-linear-gradient(left, #ccc, #333, #ccc); <br />
background-image: -moz-linear-gradient(left, #ccc, #333, #ccc); <br />
background-image: -ms-linear-gradient(left, #ccc, #333, #ccc); <br />
background-image: -o-linear-gradient(left, #ccc, #333, #ccc); <br />
}<br />
.style {<br />
font-size: 30px;<br />
/*font-family: 'Pacifico', cursive;*/<br />
/*font-family: "Times New Roman", Times, serif;*/<br />
font-family: 'Roboto', sans-serif;<br />
}<br />
<br />
<br />
<br />
<!--SPLASH PAGE--><br />
.contentBorder {<br />
border: 1px solid #AAAAAA;<br />
-webkit-border-radius: 7px;<br />
-moz-border-radius: 7px;<br />
border-radius: 7px;<br />
-webkit-box-shadow: 2px 2px 6px rgba(0, 0, 0, 0.35);<br />
-moz-box-shadow: 2px 2px 6px rgba(0, 0, 0, 0.35);<br />
box-shadow: 2px 2px 6px rgba(0, 0, 0, 0.35);<br />
}<br />
.content {<br />
/*font-family: Tahoma;*/<br />
font-family: 'Roboto', sans-serif;<br />
font-size: 12px;<br />
color: #3D3D3D;<br />
margin-bottom:60px;<br />
background-color: white;<br />
padding: 15px 60px;<br />
<br />
border: 1px solid #cccccc;<br />
-webkit-border-radius: 10px;<br />
-moz-border-radius: 10px;<br />
border-radius: 10px;<br />
-webkit-box-shadow: 1px 1px 3px rgba(0, 0, 0, 0.35);<br />
-moz-box-shadow: 1px 1px 3px rgba(0, 0, 0, 0.35);<br />
box-shadow: 1px 1px 3px rgba(0, 0, 0, 0.35);<br />
}<br />
#box5 {<br />
text-align: left;<br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:55:56Z<p>Michaelc1618: </p>
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<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ul type = "none"><br />
<li>&#9633; Register the team and have a great summer</li><br />
<p>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</p><br />
<p><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
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</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:55:44Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<!--<ul style="list-style-type:none;">--><br />
<ul type = "none"><br />
<p>&#9633; Register the team and have a great summer</p><br />
<p>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</p><br />
<p><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:53:36Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<ul style="list-style-type:none;"><br />
<p>&#9633; Register the team and have a great summer</p><br />
<p>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</p><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:53:24Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<ul style="list-style-type:none;"><br />
<li>&#9633; Register the team and have a great summer</li><br />
<p>&#9633; Successfully complete and submit this iGEM 2014 Judging form.</p><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:45:22Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<p><b>Bronze Medal Requirements</b></p><br />
<ul style="list-style-type:none;"><br />
<li>&#9633; Hello</li><br />
</ul><br />
<br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618http://2014.igem.org/Team:UCLA/JudgingTeam:UCLA/Judging2014-10-18T00:40:51Z<p>Michaelc1618: </p>
<hr />
<div>{{:Team:UCLA/Template}}<br />
<br />
<html><br />
<br />
<!--CONTENT--><br />
<div style="position:relative;width:80%;margin:auto;"><br />
<br/><br />
<h1>Judging</h1><br />
<h2>Checklist</h2><br />
<br />
<!--<br />
<p><b>Briefly describe the topics that you learned about in your safety training.</b></p><br />
<p>Our safety training covered hazardous material storage and usage, biohazard usage practices, protective personal equipment, engineering and human safety controls, and in-depth properties of select hazards, such as peroxides and flammable compounds. The laboratory safety training requirements at UCLA can be found <a href = "http://policy.ucop.edu/doc/3500598/LabSafetyTraining">here</a> </p><br />
<br />
<p><b>In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</b></p><br />
<br />
<p>In the USA, biosafety regulations are provided by the Center for Disease Control and Prevention (CDC) and the American Biological Safety Association (ABSA), and National Institute of Health (NIH)</p><br />
<br />
<br />
<h2>Project Risks</h2><br />
<br />
<p><b>Risks to the safety and health of team members, or other people working in the lab:</b></p><br />
<p><i>E. coli</i> K12 strain does not pose a major safety risk to humans. <i>Nephila clavipes</i> silk, which is the expressed protein product in our project, does not pose a safety risk either. Nevertheless, skin contact and potential ingestion should be eliminated or minimized.</p><br />
<br />
<p><b>Risks to the safety and health of the general public, particularly if any biological materials escaped from your lab:</b></p><br />
<p>Spider silk does not pose any safety or health risk to the environment if released. <i>E. coli</i> may have unpredictable impacts on the local ecosystem if released, but this is unlikely.</p><br />
<br />
<p><b>What measures are you taking to reduce these risks?</b></p><br />
<p>We chose a very safe chassis organism to use, K12 <i>E.coli</i>. We are also wearing proper Personal Protective Equipment, such as lab coats, gloves, and safety glasses when handling biological materials. Additionally, any surfaces that have been potentially contaminated by bacterial species are thoroughly cleaned using ethanol and/or bleach. </p><br />
<br />
<h2>Risks of Your Project in the Future</h2><br />
<p><b>What new risks might arise from your project's growth? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?</b></p><br />
<p>No safety risks would arise from the availability of our product or the knowledge of how to generate our product. There may be some impact to materials industries if the generation of genetically altered recombinant silk became commercially viable. For example, the natural silk industry may suffer if recombinant silk with altered properties became cheap. </p><br />
<br />
<p><b>Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks?</b></p><br />
<p>We did not utilize any design features to mitigate risks. Expressing our protein causes the chassis bacteria to be metabolically disadvantaged, and so it would likely not survive in the wild.</p><br />
--><br />
<br />
<br />
</div><br />
<br/><br />
<br/><br />
</html></div>Michaelc1618