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2014.igem.org - User contributions [en]
2024-03-29T15:45:34Z
From 2014.igem.org
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http://2014.igem.org/File:%E8%8D%89.png
File:草.png
2015-08-03T07:14:10Z
<p>Iguana: </p>
<hr />
<div></div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:49:28Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Results</font>=<br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4"><br />
STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<br />
<br />
<H1>Creating parts of IL-10α,IL-10β</H1><br />
<font size="4"><br />
IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant these sequences ,but we didn't succeed.<br />
</font><br />
<br><br />
<br><br />
<br />
<br />
<br />
<H1>Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:48:53Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Results</font>=<br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4"><br />
STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of IL-10α,IL-10β</H1><br />
<font size="4"><br />
IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant these sequences ,but we didn't succeed.<br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Safety
Team:KAIT Japan/Safety
2014-10-17T09:47:55Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|} <br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Safety</font>=<br />
<br><br />
<font size="4"><br />
Before answering these questions on your team Safety page, be sure to read the Safety in iGEM page. And the FAQ section below.<br />
<br />
<br />
'''1. Your Training''' <br />
<br />
:a) Have your team members received any safety training yet?<br />
<br />
::Yes, we have already received safety training.<br />
<br />
<br />
:b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.<br />
<br />
::We took biosafety training by the class of the university.<br />
::We learned how to use laboratory instruments such as clean bench, autoclave, and risk of biological materials.<br />
<br />
<br />
:c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.<br />
<br />
::http://kw.kait.jp/kw/top/index2.aspx<br />
<br />
<br />
'''2. Your Local Rules and Regulations''' <br />
<br />
:a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety,a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.<br />
<br />
::The president of Kanagawa Institute of Technology has the responsibility of the biological safety.<br />
::And our experiment plan gets the approval of the safe committee of Kanagawa Institute of Technology. <br />
::The president of Kanagawa Institute of Technology approves it.<br />
<br />
<br />
:b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.<br />
<br />
::http://kw.kait.jp/kw/top/index2.aspx<br />
:These guidelines are made in reference to the standard to appoint of the country.<br />
:The URL is http://www.lifescience.mext.go.jp/files/pdf/n815_01.pdf .<br />
<br />
<br />
:c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.<br />
<br />
::http://www.lifescience.mext.go.jp/files/pdf/n815_01.pdf<br />
<br />
<br />
'''3. The Organisms and Parts that You Use''' <br />
<br />
:Please visit this page to download a blank copy of the spreadsheet for question 3. <br />
:(If you need a CSV version instead of XLS, visit this page.)<br />
<br />
::https://2014.igem.org/File:TeamName_Safety2014_Spreadsheet.xls<br />
<br />
<br />
'''4. Risks of Your Project Now''' <br />
<br />
:Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.<br />
<br />
:a)Risks to the safety and health of team members, or other people working in the lab:<br />
<br />
::We used E.coli Pro 5-alpha. It doesn't cause irritation effect on the skin. And It doesn't sensitize us.<br />
<br />
<br />
:b)Risks to the safety and health of the general public (if any biological materials escaped from your lab):<br />
<br />
::If our organisms escape, people take it into their body, it may fall into autoimmune disease by Th1 increasing excessively.<br />
::We used E.coli Pro 5-alpha. It doesn't cause irritation effect on the skin. And It doesn't sensitize us.<br />
::Aquatic toxicity: Not harmful to the aquatic environment.<br />
::We have sterilized waste and used materials .<br />
<br />
<br />
:c)Risks to security through malicious mis-use by individuals, groups, or countries:<br />
<br />
::We discard the experimental dust after autocleave. Therefore there cannot be the thing leaking outside.<br />
<br />
<br />
:d)What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)<br />
<br />
:We put on lab coats , rubber gloves to prevent us from getting hurt.<br />
:We have sterilized our used materials by autoclave and ethanol.<br />
:We have experimented in clean bench to prevent our organisms from escaping from lab. <br />
:We have sterilized waste and used materials to minimize the risk of our organism escaping from Lab.<br />
<br />
<br />
'''5. Risks of Your Project in the Future''' <br />
<br />
:What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.<br />
<br />
:a)What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) <br />
:Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?<br />
<br />
::・It may fall into autoimmune disease by Th1 increasing excessively.<br />
::・α-hemolysin (Used as a tag to start it outside IL-12) provides hemolysis.<br />
::・When a human being took E coli, it is concerned whether you are safe.<br />
<br />
<br />
:b)Does your project currently include any design features to reduce risks? <br />
:Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.)<br />
:Such features are not required for an iGEM project, but many teams choose to explore them.<br />
<br />
::Yes, our project include it.<br />
::Yeast which we make by this project does not transcription if it does not receive IL-5.<br />
</font></div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Human_Practice
Team:KAIT Japan/Human Practice
2014-10-17T09:46:40Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|} <br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Human Practice</font>=<br />
<br><br />
<br />
<p><br />
:<font size=6>We announced the poster under the theme of <br />
::<font color="red">"genetic modification"</font color> in the open campus of our university.<br />
</font size><br />
</p><br />
<br><br />
<br />
:[[File:Ggf.jpg]][[File:Ffd.jpg]][[File:Ppo.jpg]]<br />
<br><br />
<br><br />
:<font size=6>And,I took the questionnaire after this poster announcement.</font size><br />
<br><br />
<br><br />
<font size=5><br />
:<Questionnaire contents><br />
:1)How did you think about genetic modification?<br />
:2)Are you interested about genetic modification?<br />
:3)Was our explanation easy to understand?<br />
:4)From today's explanation, how did you think about genetic modification?<br />
:5)When heard genetic modification, how do you think?<br />
:6)Are there an opinion and the impression?<br />
</font size><br />
<br><br />
<br><br />
<br><br />
<br><br />
::<font size=5><Questionnaire results></font size><br />
::::[[File:1アンケート.jpg]] [[File:2アンケート.jpg]]<br />
<br><br />
::::[[File:3アンケート.jpg]] [[File:4アンケート.jpg]]<br />
<br><br />
<font size=5><br />
:::5)A・Fear for the food.<br />
<br><br />
:::6)A・It's been a good education.I felt genetic modification close<br />
</font size></div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Safety
Team:KAIT Japan/Safety
2014-10-17T09:46:04Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|} <br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Safety</font>=<br />
<br><br />
Before answering these questions on your team Safety page, be sure to read the Safety in iGEM page. And the FAQ section below.<br />
<br />
<br />
'''1. Your Training''' <br />
<br />
:a) Have your team members received any safety training yet?<br />
<br />
::Yes, we have already received safety training.<br />
<br />
<br />
:b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.<br />
<br />
::We took biosafety training by the class of the university.<br />
::We learned how to use laboratory instruments such as clean bench, autoclave, and risk of biological materials.<br />
<br />
<br />
:c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.<br />
<br />
::http://kw.kait.jp/kw/top/index2.aspx<br />
<br />
<br />
'''2. Your Local Rules and Regulations''' <br />
<br />
:a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety,a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.<br />
<br />
::The president of Kanagawa Institute of Technology has the responsibility of the biological safety.<br />
::And our experiment plan gets the approval of the safe committee of Kanagawa Institute of Technology. <br />
::The president of Kanagawa Institute of Technology approves it.<br />
<br />
<br />
:b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.<br />
<br />
::http://kw.kait.jp/kw/top/index2.aspx<br />
:These guidelines are made in reference to the standard to appoint of the country.<br />
:The URL is http://www.lifescience.mext.go.jp/files/pdf/n815_01.pdf .<br />
<br />
<br />
:c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.<br />
<br />
::http://www.lifescience.mext.go.jp/files/pdf/n815_01.pdf<br />
<br />
<br />
'''3. The Organisms and Parts that You Use''' <br />
<br />
:Please visit this page to download a blank copy of the spreadsheet for question 3. <br />
:(If you need a CSV version instead of XLS, visit this page.)<br />
<br />
::https://2014.igem.org/File:TeamName_Safety2014_Spreadsheet.xls<br />
<br />
<br />
'''4. Risks of Your Project Now''' <br />
<br />
:Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.<br />
<br />
:a)Risks to the safety and health of team members, or other people working in the lab:<br />
<br />
::We used E.coli Pro 5-alpha. It doesn't cause irritation effect on the skin. And It doesn't sensitize us.<br />
<br />
<br />
:b)Risks to the safety and health of the general public (if any biological materials escaped from your lab):<br />
<br />
::If our organisms escape, people take it into their body, it may fall into autoimmune disease by Th1 increasing excessively.<br />
::We used E.coli Pro 5-alpha. It doesn't cause irritation effect on the skin. And It doesn't sensitize us.<br />
::Aquatic toxicity: Not harmful to the aquatic environment.<br />
::We have sterilized waste and used materials .<br />
<br />
<br />
:c)Risks to security through malicious mis-use by individuals, groups, or countries:<br />
<br />
::We discard the experimental dust after autocleave. Therefore there cannot be the thing leaking outside.<br />
<br />
<br />
:d)What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)<br />
<br />
:We put on lab coats , rubber gloves to prevent us from getting hurt.<br />
:We have sterilized our used materials by autoclave and ethanol.<br />
:We have experimented in clean bench to prevent our organisms from escaping from lab. <br />
:We have sterilized waste and used materials to minimize the risk of our organism escaping from Lab.<br />
<br />
<br />
'''5. Risks of Your Project in the Future''' <br />
<br />
:What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.<br />
<br />
:a)What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) <br />
:Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?<br />
<br />
::・It may fall into autoimmune disease by Th1 increasing excessively.<br />
::・α-hemolysin (Used as a tag to start it outside IL-12) provides hemolysis.<br />
::・When a human being took E coli, it is concerned whether you are safe.<br />
<br />
<br />
:b)Does your project currently include any design features to reduce risks? <br />
:Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.)<br />
:Such features are not required for an iGEM project, but many teams choose to explore them.<br />
<br />
::Yes, our project include it.<br />
::Yeast which we make by this project does not transcription if it does not receive IL-5.</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Safety
Team:KAIT Japan/Safety
2014-10-17T09:45:43Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|} <br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=,font size="7"S>afety</font><br />
<br><br />
Before answering these questions on your team Safety page, be sure to read the Safety in iGEM page. And the FAQ section below.<br />
<br />
<br />
'''1. Your Training''' <br />
<br />
:a) Have your team members received any safety training yet?<br />
<br />
::Yes, we have already received safety training.<br />
<br />
<br />
:b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.<br />
<br />
::We took biosafety training by the class of the university.<br />
::We learned how to use laboratory instruments such as clean bench, autoclave, and risk of biological materials.<br />
<br />
<br />
:c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.<br />
<br />
::http://kw.kait.jp/kw/top/index2.aspx<br />
<br />
<br />
'''2. Your Local Rules and Regulations''' <br />
<br />
:a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety,a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.<br />
<br />
::The president of Kanagawa Institute of Technology has the responsibility of the biological safety.<br />
::And our experiment plan gets the approval of the safe committee of Kanagawa Institute of Technology. <br />
::The president of Kanagawa Institute of Technology approves it.<br />
<br />
<br />
:b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.<br />
<br />
::http://kw.kait.jp/kw/top/index2.aspx<br />
:These guidelines are made in reference to the standard to appoint of the country.<br />
:The URL is http://www.lifescience.mext.go.jp/files/pdf/n815_01.pdf .<br />
<br />
<br />
:c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.<br />
<br />
::http://www.lifescience.mext.go.jp/files/pdf/n815_01.pdf<br />
<br />
<br />
'''3. The Organisms and Parts that You Use''' <br />
<br />
:Please visit this page to download a blank copy of the spreadsheet for question 3. <br />
:(If you need a CSV version instead of XLS, visit this page.)<br />
<br />
::https://2014.igem.org/File:TeamName_Safety2014_Spreadsheet.xls<br />
<br />
<br />
'''4. Risks of Your Project Now''' <br />
<br />
:Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.<br />
<br />
:a)Risks to the safety and health of team members, or other people working in the lab:<br />
<br />
::We used E.coli Pro 5-alpha. It doesn't cause irritation effect on the skin. And It doesn't sensitize us.<br />
<br />
<br />
:b)Risks to the safety and health of the general public (if any biological materials escaped from your lab):<br />
<br />
::If our organisms escape, people take it into their body, it may fall into autoimmune disease by Th1 increasing excessively.<br />
::We used E.coli Pro 5-alpha. It doesn't cause irritation effect on the skin. And It doesn't sensitize us.<br />
::Aquatic toxicity: Not harmful to the aquatic environment.<br />
::We have sterilized waste and used materials .<br />
<br />
<br />
:c)Risks to security through malicious mis-use by individuals, groups, or countries:<br />
<br />
::We discard the experimental dust after autocleave. Therefore there cannot be the thing leaking outside.<br />
<br />
<br />
:d)What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)<br />
<br />
:We put on lab coats , rubber gloves to prevent us from getting hurt.<br />
:We have sterilized our used materials by autoclave and ethanol.<br />
:We have experimented in clean bench to prevent our organisms from escaping from lab. <br />
:We have sterilized waste and used materials to minimize the risk of our organism escaping from Lab.<br />
<br />
<br />
'''5. Risks of Your Project in the Future''' <br />
<br />
:What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.<br />
<br />
:a)What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) <br />
:Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?<br />
<br />
::・It may fall into autoimmune disease by Th1 increasing excessively.<br />
::・α-hemolysin (Used as a tag to start it outside IL-12) provides hemolysis.<br />
::・When a human being took E coli, it is concerned whether you are safe.<br />
<br />
<br />
:b)Does your project currently include any design features to reduce risks? <br />
:Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.)<br />
:Such features are not required for an iGEM project, but many teams choose to explore them.<br />
<br />
::Yes, our project include it.<br />
::Yeast which we make by this project does not transcription if it does not receive IL-5.</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:45:06Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Results</font>=<br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4"><br />
STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of IL-10α,IL-10β</H1><br />
<font size="4"><br />
IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant these sequences ,but we didn't succeed.<br />
</font><br />
<br><br />
<br><br />
<br />
<br />
<br />
<br />
<H1>Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Notebook
Team:KAIT Japan/Notebook
2014-10-17T09:44:23Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
| <br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br><br />
__NOTOC__<br />
=<font size ="7">Notebook</font>=<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of GFP and HlyA</font>'''==<br />
:<big>'''Date:8/22'''</big> The refinement of DNA and PCR and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction<br />
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation<br />
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction<br />
:<big>'''Date:8/27'''</big> Electrophoresed the DNA that We did refinement on August 26 to confirmed it<br />
:<big>'''Date:8/28'''</big> Check the HlyA and GFP for colony PCR<br />
:<big>'''Date:8/29'''</big> DNA purify the HlyA and GFP <br />
:<big>'''Date:8/29'''</big> Ligation product and check for PCR<br />
:<big>'''Date:9/1'''</big> Build replica the HlyA and GFP<br />
:<big>'''Date:9/1'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/2'''</big> Restriction enzyme treatment the HlyA and GFP in 9/1 was NANOSEP<br />
:<big>'''Date:9/3'''</big> Ligation and PCR, DNA purification the HlyA and GFP <br />
:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/5'''</big> Restriction enzyme treatment the vector<br />
:<big>'''Date:9/5'''</big> Ligation the vector and insert. So transformation.<br />
:<big>'''Date:9/9'''</big> Check the HiyA and GFP replica.Ligation and transformation the vector and insert DNA.<br />
:<big>'''Date:9/10'''</big> Transformation in 9/9 check the colony PCR.TA cloning the HlyA+GFP.<br />
:<big>'''Date:9/11'''</big> Ligation the vector and insert DNA.<br />
:<big>'''Date:9/12~9/15'''</big> Transformation the Ligation product.<br />
:<big>'''Date:9/16'''</big> Noticed wrong the primer.so restart from the First.<br />
:<big>'''Date:9/17'''</big> PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment.<br />
:<big>'''Date:9/18'''</big> Ligation the GFP and HlyA.<br />
:<big>'''Date:9/19'''</big> Electrophoresis the ligation the GFP+HlyA in 9/18 <br />
:<big>'''Date:9/20'''</big> DNA purify and PCR<br />
:<big>'''Date:9/21'''</big> PCR and DNA purify <br />
:<big>'''Date:9/22'''</big> Ligation the GFP+HlyA<br />
:<big>'''Date:9/23'''</big> PCR and DNA purify<br />
:<big>'''Date:9/24'''</big> TA Cloning was GFP and HlyA<br />
:<big>'''Date:9/26'''</big> Restriction enzyme treatment the ligation the GFP+HlyA in 9/22<br />
:<big>'''Date:9/27'''</big> Check the TA cloning of GFP+HlyA<br />
:<big>'''Date:9/29'''</big> Restriction enzyme treatment the GFP+HlyA and the pSBIC3<br />
:<big>'''Date:9/29'''</big> The vector and the insert were NANOSEP and lectrophoresis<br />
:<big>'''Date:9/30'''</big> The vector and the insert were ligation and transformation<br />
:<big>'''Date:9/30'''</big> The GFP+HlyA was PCR and DNA purify <br />
:<big>'''Date:10/1'''</big> Electrophoresis the GFP+HlyA was PCR and DNA purify in 9/30<br />
:<big>'''Date:10/2'''</big> Restriction enzyme treatment and ligation the GFP+HlyA<br />
:<big>'''Date:10/3'''</big> Check the ligation in 10/2 <br />
:<big>'''Date:10/3'''</big> Transformation the plasmid<br />
:<big>'''Date:10/5'''</big> Colony PCR<br />
:<big>'''Date:10/6'''</big> Miniprep and Electrophoresis<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max) <br />
:<big>'''Date:9/2'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/5~9/8'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/9'''</big> Sequence(to To confirm whether variation happened in DNA of STAT3 )<br />
:<big>'''Date:9/10~9/12'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/13~9/15'''</big> DNA purification and electrophoresis<br />
:<big>'''Date:9/16~9/17'''</big> DNA purification and colony PCR(Failure)<br />
:<big>'''Date:9/18'''</big> colony PCR(Failure)<br />
:<big>'''Date:9/19~9/22'''</big> colony PCR and electrophoresis <br />
:<big>'''Date:9/23'''</big> PCR and Electrophoresis(We found out that DNA polymerase was malfunction)<br />
:<big>'''Date:9/24'''</big> PCR and Electrophoresis and DNA DNA purification <br />
:<big>'''Date:9/25'''</big> PCR(to find annealing temperature)<br />
:<big>'''Date:9/26~9/30'''</big> DNA purification and electrophoresis(to use DNA for the sequence)<br />
:<big>'''Date:10/1'''</big> Dilution after Concentration measurement of DNA<br />
:<big>'''Date:10/2'''</big> Sequence(to check mutation of DNA)/The variation was not found.<br />
:<big>'''Date:10/4'''</big> PCR and electrophoresis <br />
:<big>'''Date:10/5~10/7'''</big> Colony PCR and DNA purification<br />
:<big>'''Date:10/8'''</big> Concentration measurement of DNA<br />
:<big>'''Date:10/9'''</big> Sequence(to check mutation of DNA)/The variation was not found<br />
<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max)<br />
:<big>'''Date:9/2'''</big> PCR<br />
:<big>'''Date:9/4'''</big> Transformation<br />
:<big>'''Date:9/5'''</big> Colony PCR<br />
:<big>'''Date:9/7'''</big> ColonyPCR<br />
:<big>'''Date:9/8'''</big> DNA extraction and Sequence(to To confirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/9'''</big> DNA sequence<br />
:<big>'''Date:9/10'''</big> PCR and Electtrophoresis<br />
:<big>'''Date:9/11'''</big> Colony PCR <br />
:<big>'''Date:9/12'''</big> DNA extraction<br />
:<big>'''Date:9/13'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/15'''</big> ultrafiltration<br />
:<big>'''Date:9/16'''</big> DNA sequence(to comfirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/20'''</big> PCR<br />
:<big>'''Date:9/23'''</big> Colony PCR<br />
:<big>'''Date:9/24'''</big> Electrophoresis<br />
:<big>'''Date:9/26'''</big> ultrafiltration<br />
:<big>'''Date:9/27'''</big> PCR and colony PCR<br />
:<big>'''Date:9/29'''</big> Electrophoresis<br />
:<big>'''Date:9/30'''</big> DNA Extraction<br />
:<big>'''Date:10/1'''</big> Colony PCR and DNA extraction<br />
:<big>'''Date:10/2'''</big> Electrophoresis<br />
:<big>'''Date:10/3'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/4'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/5'''</big> Electrophoresis<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
:<big>'''Date:9/16'''</big> PCR:Ara,AraPro,HRV Transformation:IL-5Ra,IL-5Rb<br />
:<big>'''Date:9/17'''</big> Restriction enzyme treatment and insert to TA vector and transformation<br />
:<big>'''Date:9/18'''</big> transformation<br />
:<big>'''Date:9/19'''</big> miniprep and colony PCR<br />
:<big>'''Date:9/20~9/22'''</big> PCR<br />
:<big>'''Date:9/23'''</big> TA Cloning<br />
:<big>'''Date:9/24'''</big> TA Cloning and PCR<br />
:<big>'''Date:9/25'''</big> Restriction enzyme treatment and PCR<br />
:<big>'''Date:9/26'''</big> Colony PCR and Ligation<br />
:<big>'''Date:9/27'''</big> TA Cloning and Ligation and colony PCR<br />
:<big>'''Date:9/28'''</big> PCR<br />
:<big>'''Date:9/29'''</big> colony PCR and PCR and DNA refinement and Ligation<br />
:<big>'''Date:9/30'''</big> DNA refinement and Ligation<br />
:<big>'''Date:10/1'''</big> Restriction enzyme treatment and Ligation<br />
:<big>'''Date:10/2'''</big> PCR and Ligation and DNA refinement <br />
:<big>'''Date:10/3'''</big> Ligation<br />
:<big>'''Date:10/4'''</big> Ligation<br />
:<big>'''Date:10/7'''</big> PCR<br />
:<big>'''Date:10/8'''</big> PCR and Restriction enzyme <br />
:<big>'''Date:10/9'''</big> DNA refinement and Restriction enzyme <br />
:<big>'''Date:10/10'''</big> Phoshatase treatment <br />
:<big>'''Date:10/11'''</big> DNA refinement and Ligation and Restriction enzyme treatment<br />
:<big>'''Date:10/12'''</big> DNA refinement and PCR and Ligation and phoshatase treatment <br />
<br />
<br />
<br />
<br />
<br />
<br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Team
Team:KAIT Japan/Team
2014-10-17T09:43:51Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|} <br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
__NOTOC__<br />
=<font size="7">Team</font>=<br />
:[https://igem.org/Team.cgi '''Official Team Profile''']<br />
== '''Team member''' ==<br />
<big>'''Instructor'''</big><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Iida2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yasuhiro Iida'''</big><br><br />
:'''''Associate Professor'''''<br><br />
:[http://iida.biokait.jp/iida/welcome.html Iida Lab]<br><br />
:[http://www.biokait.jp/biokait/Welcome.html Applied Bioscience Course]<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<big>'''Main Members'''</big><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Tosu.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Sachina Tosu'''</big><br><br />
:'''''Team Leader'''''<br><br />
:Third Year Student<br><br />
:Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Saijo.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Miyuki Saijo'''</big><br />
<br><br />
:Third Year Student<br><br />
:Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Maeda-Shota2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Shota Maeda'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Takamasa Tanaka'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Nishimura2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Toshitaka Nishimura'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Ogawa2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Taichi Ogawa'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Kanai2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Kunifumi Kanai'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course <br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Sotokawa.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Kanna Sotokawa'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Noguchi.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Shohei Noguchi'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Saegusa.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Momoko Saegusa'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Sugahara.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Keisuke Sugahara'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Watanabe.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yuuki Watanabe'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Dejima.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Ami Dejima'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Nomura.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Minami Nomura'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Okano.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Maria Okano'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Tanimura.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yukina Tanimura'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Kimura.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Ayaka Kimura'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Syoda.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Daiki Syoda'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Kubota.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Naoya Kubota'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Takahashi.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Amon Takahashi'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yuuki Kageyama'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Hashimoto.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yuki Hashimoto'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Chiba.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Takuya Chiba'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Suzuki.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yasuhiro Suzuki'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Toma.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Hiroki Toma'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Team
Team:KAIT Japan/Team
2014-10-17T09:43:40Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|} <br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
__NOTOC__<br />
=<font size="7">Team</font><br />
:[https://igem.org/Team.cgi '''Official Team Profile''']<br />
== '''Team member''' ==<br />
<big>'''Instructor'''</big><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Iida2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yasuhiro Iida'''</big><br><br />
:'''''Associate Professor'''''<br><br />
:[http://iida.biokait.jp/iida/welcome.html Iida Lab]<br><br />
:[http://www.biokait.jp/biokait/Welcome.html Applied Bioscience Course]<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<big>'''Main Members'''</big><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Tosu.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Sachina Tosu'''</big><br><br />
:'''''Team Leader'''''<br><br />
:Third Year Student<br><br />
:Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Saijo.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Miyuki Saijo'''</big><br />
<br><br />
:Third Year Student<br><br />
:Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Maeda-Shota2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Shota Maeda'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Takamasa Tanaka'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Nishimura2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Toshitaka Nishimura'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Ogawa2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Taichi Ogawa'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT Japan-Kanai2.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Kunifumi Kanai'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course <br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Sotokawa.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Kanna Sotokawa'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Noguchi.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Shohei Noguchi'''</big><br />
<br><br />
Third Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Saegusa.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Momoko Saegusa'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Sugahara.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Keisuke Sugahara'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Watanabe.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yuuki Watanabe'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Dejima.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Ami Dejima'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Nomura.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Minami Nomura'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
<br><br />
{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Okano.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Maria Okano'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Tanimura.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yukina Tanimura'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
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{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
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[[File:KAIT japan-Kimura.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Ayaka Kimura'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
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[[File:KAIT japan-Syoda.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Daiki Syoda'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
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{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
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[[File:KAIT japan-Kubota.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Naoya Kubota'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Takahashi.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Amon Takahashi'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
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<br><br />
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{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
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| style="border-left:3px dotted #99F" |<br />
<big>'''Yuuki Kageyama'''</big><br />
<br><br />
Second Year Student<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Hashimoto.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yuki Hashimoto'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
<br><br />
<br><br />
<br><br />
<br><br />
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{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
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[[File:KAIT japan-Chiba.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Takuya Chiba'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
{| align="right" style="width:49%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:KAIT japan-Suzuki.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Yasuhiro Suzuki'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}<br />
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{| align="left" style="width:49%; margin-left:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
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[[File:KAIT japan-Toma.jpg]]<br />
| style="border-left:3px dotted #99F" |<br />
<big>'''Hiroki Toma'''</big><br />
<br><br />
Freshman<br />
<br><br />
Applied Bioscience Course<br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Parts
Team:KAIT Japan/Parts
2014-10-17T09:42:47Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
| <br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
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|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
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[[Team:KAIT_Japan/Team|Team]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
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[[Team:KAIT_Japan/Project|Project]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
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[[Team:KAIT_Japan/Parts|Parts]]<br />
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|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
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[[Team:KAIT_Japan/Safety|Safety]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
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{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1><font size="7">Parts</font></H1><br />
::::<groupparts>iGEM014 KAIT_Japan</groupparts><br />
<br />
<br></div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Project
Team:KAIT Japan/Project
2014-10-17T09:42:08Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
| <br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
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[[Team:KAIT_Japan/Parts|Parts]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
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{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
=<font size="7">Project</font>=<br />
<br><br />
=='''<font size="5">Back graund</font>'''==<br />
<font size="4"><br />
・The secondary immune response<br><br />
As a person's immune system encounters foreign substances (antigens), the components of acquired immunity learn the best way to attack each antigen and develop a memory for that antigen.<br />
The immune system is turned on when the antigen presenting cell (APC) such as the dendritic cell is taken in by an endocytosis.<br />
The antigen taken in is broken down to peptide by lysosome.<br />
The antigenic peptides form a complx when MHC class Ⅱ molecules composed in endoplasmic reticulum and are carried to the cell surface. The T cell Receptor (TcR) of the T cell (CD4 cell) combines with this complex. This becomes the first signal.<br />
Furthermore, CD28 of the CD4 cell combines with B4 of the APC.to be the supporting signal; CD4 cell is activated by these two signals.<br />
The activated T cell_(CD4+ cell) differentiates to Th2 cell by stimulation of interleukin-4(IL-4) proteins or to Th1 cell by IL-12,respectively.<br />
Th1 participates in cell-mediated innunity to produces IFN-γand IL-2 and activates a macrophage and a killer cell.Th2 ,on the other hand,participates in humoral immunity-to produces IL-4, IL-5,_IL-10 and activates B cell and a mast cell.<br />
In this way, Th1 and Th2 play important roles in immune reaction.<br />
<br><br />
<br><br />
・Interaction between Th1 and Th2<br><br />
Depending on pathogens and stimulation of immunitys,a kind of the immune response is decided either the Th1 predominance or the Th2 predominance.<br />
When a virus invades the body, differentiation to Th1 is promoted by Il-12 produced by APC, and Th1 produce IFN-γ for phylaxis. At the same time, Th2 is controlled by IFN-γ, and the production of cytokine promoting mast cells and eosinophil is also controlled . On the other hand, when an antigen invades body, differentiation to Th2 is promoted by IL-4 . Th2 produces IL-4, and activates B cells to produce an antibody (IgE,IgG). Moreover, Th2 produces IL-5 and promotes eosinophil work. At the same time, Th1 is controlled by IL-4, and the production of cytokine promoting the function of macrophages is also controlled.<br />
In this way, Th1 and Th2 usually keep the mutual balance to control immune response. (Th1/Th2 balance) <br />
When this balance is lost and declined to the Th2 predominance, IL-5 is secreted excessively from Th2, and eosonophil and mast cells are activated. Subsequently, that causes excessive secretion of inflammatory materials such as histamine or leukotriene when an antigen is connected to those activated cells having antibody bound to through Fc receptor (FcR). This inflammatory material and antibody produced excessively are related to allergic reactions.<br />
The allergic reactions are classified from type Ⅰto Ⅳ;these reactions can occur independently,or multiple types may be taking place at the same time.<br />
Above all, type Ⅰ reactions is called immediate hypersensitivity, and the onset appears in a very short time.<br />
_Examples of this type include bronchial asthma and atopic dermatitis<br />
There are the people suffering by these allergic diseases.<br />
Therefore, we thought that keeping the Th1/Th2 balance by increasing IL-12 which participates in Th1 cytodifferentiation can restrain allergic reactions.<br />
</font><br />
<br><br />
[[File:New ダブルチャージ.jpg]]<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="5">Link Light Assay</font>'''==<br />
<font size="4"><br />
A variety of protein interaction analyses have been developed. Linklight Assay is a novel method based on an inactive permuted protein which can be activated by a protease to generate stable and sensitive signals for monitoring protein interaction.<br />
Linklight Assay consists of two components: A protein A fused to a Tobacco Etch Virus (TEV) protease and a protein B fused to a permuted luciferase (pLuc) containing a TEV protease cleavage sequence. An inactive pLuc can be created by breaking a luciferase into two fragments, rearranging the fragment order as the N-terminal fragment moved to C-terminus and C-terminal fragment moved to the N-terminus. and connecting them with a TEV protease cleavage sequence. After interaction between protein A and B, inactive pLuc is cleaved, and the cleaved luciferase fragments spontaneously refold to reconsititute an active luciferase. Active luciferase works as reporter.<br />
Linklight Assay provides simple, robust, economic, and sensitive methods to detect protein interaction in live cells with immediate and stable signals.<br />
In this study applied this technology to make IL-5 independent expression system. We will explain our strategy for Ecoli receiving IL5, and for producing IL-12 in Strategy Page.<br />
</font><br />
<br><br />
[[File:New ビックカメラ.jpg]]<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="5">HRV protease</font>'''==<br />
<font size="4"><br />
TEV protease is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). Due to its high sequence specificity, it is frequently used for the controlled cleavage of fusion proteins.<br />
Human Rhinovirus (HRV) 14 3C protease is a cysteine protease used to remove fusion from proteins with the HRV14 3C cleavage sequence, like TEV protease. HRV14 3C protease has also high specific recognition sequence to Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro as the substrate. The 22 kDa protease get its optimal activity at 4 ℃, but also shows a highiy cleavage rate at 37℃.<br />
HRV14 3C protease is very similar to TEV protease. So, we thought that HRV14 3C protease can cleave protease cleavage sequence which connects IL5 receptor and AraC in the same way TEV protease cleavage protein B fused to a pLuc containing a TEV protease cleavage sequence in linklight assay.<br />
</font><br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="5">Methodology</font>'''==<br />
<font size="4"><br />
Strategy for ecoli to receive IL5, and to produces IL-12.<br />
Applying Linklight assay, our goal is to create an ecoli which receives IL-5,and produces IL-12.<br />
IL-5 is an interleukin produced by T helper 2 cells, mast cells and eosinophils. Through binding to the IL-5 receptor, IL-5 stimulates B cells growth and increases immunoglobulin secretion. It is also a key mediator in eosinophils activation. <br />
IL-5receptor consists of a heterodimer of an alpha subunit and a beta subunit. The IL-5 receptor alpha (IL-5Ra) is specific to IL-5. The beta chain (IL-5Rb) is the common beta chain of receptors for IL-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF). After IL-5 binds to IL-5Ra, IL-5Rb meets IL-5 and form heterodimer with alpha subunit. If IL-5 does not bind to IL-5Ra, IL-5Rb cannot form heterodimer with alpha subunit.<br><br />
<br />
The method applied for Ecoli:to receive IL-5and to produce IL-12 is as follows:<br />
Fusing IL-5Ra and IL-5Rb with AraC which contains a HRV protease cleavage sequence and HRV protease respectively. If heterodimer of IL-5Ra and IL-5Rb are formed by IL-5, AraC containing cleavage sequence and HRV are forced into close proximity. HRV protease recognizes cleavage sequence connecting IL5Ra and AraC and cleaves it. AraC is released from IL5Ra by HRV protease. After released from IL5Ra, AraC regulates expression of IL12 coding gene downstream of AraC promoter. When arabinose is present, AraC acts an activator, and activates expression of IL12.HlyA C-terminal signal is a protein secrete tag. So IL12 which has HlyA secrete tag are secreted outside an ecoli. In the lour experiment, we use GFP instead of IL12 to simplify result. This strategy enable for ecoli to receive IL5 and produce IL12.<br />
</font><br />
<br><br />
[[File:New 臨界ブラ木.jpg]]</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Notebook
Team:KAIT Japan/Notebook
2014-10-17T09:40:58Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
| <br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
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{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
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|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br><br />
__NOTOC__<br />
=<font size ="7">'''Notebook'''</font>=<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of GFP and HlyA</font>'''==<br />
:<big>'''Date:8/22'''</big> The refinement of DNA and PCR and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction<br />
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation<br />
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction<br />
:<big>'''Date:8/27'''</big> Electrophoresed the DNA that We did refinement on August 26 to confirmed it<br />
:<big>'''Date:8/28'''</big> Check the HlyA and GFP for colony PCR<br />
:<big>'''Date:8/29'''</big> DNA purify the HlyA and GFP <br />
:<big>'''Date:8/29'''</big> Ligation product and check for PCR<br />
:<big>'''Date:9/1'''</big> Build replica the HlyA and GFP<br />
:<big>'''Date:9/1'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/2'''</big> Restriction enzyme treatment the HlyA and GFP in 9/1 was NANOSEP<br />
:<big>'''Date:9/3'''</big> Ligation and PCR, DNA purification the HlyA and GFP <br />
:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/5'''</big> Restriction enzyme treatment the vector<br />
:<big>'''Date:9/5'''</big> Ligation the vector and insert. So transformation.<br />
:<big>'''Date:9/9'''</big> Check the HiyA and GFP replica.Ligation and transformation the vector and insert DNA.<br />
:<big>'''Date:9/10'''</big> Transformation in 9/9 check the colony PCR.TA cloning the HlyA+GFP.<br />
:<big>'''Date:9/11'''</big> Ligation the vector and insert DNA.<br />
:<big>'''Date:9/12~9/15'''</big> Transformation the Ligation product.<br />
:<big>'''Date:9/16'''</big> Noticed wrong the primer.so restart from the First.<br />
:<big>'''Date:9/17'''</big> PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment.<br />
:<big>'''Date:9/18'''</big> Ligation the GFP and HlyA.<br />
:<big>'''Date:9/19'''</big> Electrophoresis the ligation the GFP+HlyA in 9/18 <br />
:<big>'''Date:9/20'''</big> DNA purify and PCR<br />
:<big>'''Date:9/21'''</big> PCR and DNA purify <br />
:<big>'''Date:9/22'''</big> Ligation the GFP+HlyA<br />
:<big>'''Date:9/23'''</big> PCR and DNA purify<br />
:<big>'''Date:9/24'''</big> TA Cloning was GFP and HlyA<br />
:<big>'''Date:9/26'''</big> Restriction enzyme treatment the ligation the GFP+HlyA in 9/22<br />
:<big>'''Date:9/27'''</big> Check the TA cloning of GFP+HlyA<br />
:<big>'''Date:9/29'''</big> Restriction enzyme treatment the GFP+HlyA and the pSBIC3<br />
:<big>'''Date:9/29'''</big> The vector and the insert were NANOSEP and lectrophoresis<br />
:<big>'''Date:9/30'''</big> The vector and the insert were ligation and transformation<br />
:<big>'''Date:9/30'''</big> The GFP+HlyA was PCR and DNA purify <br />
:<big>'''Date:10/1'''</big> Electrophoresis the GFP+HlyA was PCR and DNA purify in 9/30<br />
:<big>'''Date:10/2'''</big> Restriction enzyme treatment and ligation the GFP+HlyA<br />
:<big>'''Date:10/3'''</big> Check the ligation in 10/2 <br />
:<big>'''Date:10/3'''</big> Transformation the plasmid<br />
:<big>'''Date:10/5'''</big> Colony PCR<br />
:<big>'''Date:10/6'''</big> Miniprep and Electrophoresis<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max) <br />
:<big>'''Date:9/2'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/5~9/8'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/9'''</big> Sequence(to To confirm whether variation happened in DNA of STAT3 )<br />
:<big>'''Date:9/10~9/12'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/13~9/15'''</big> DNA purification and electrophoresis<br />
:<big>'''Date:9/16~9/17'''</big> DNA purification and colony PCR(Failure)<br />
:<big>'''Date:9/18'''</big> colony PCR(Failure)<br />
:<big>'''Date:9/19~9/22'''</big> colony PCR and electrophoresis <br />
:<big>'''Date:9/23'''</big> PCR and Electrophoresis(We found out that DNA polymerase was malfunction)<br />
:<big>'''Date:9/24'''</big> PCR and Electrophoresis and DNA DNA purification <br />
:<big>'''Date:9/25'''</big> PCR(to find annealing temperature)<br />
:<big>'''Date:9/26~9/30'''</big> DNA purification and electrophoresis(to use DNA for the sequence)<br />
:<big>'''Date:10/1'''</big> Dilution after Concentration measurement of DNA<br />
:<big>'''Date:10/2'''</big> Sequence(to check mutation of DNA)/The variation was not found.<br />
:<big>'''Date:10/4'''</big> PCR and electrophoresis <br />
:<big>'''Date:10/5~10/7'''</big> Colony PCR and DNA purification<br />
:<big>'''Date:10/8'''</big> Concentration measurement of DNA<br />
:<big>'''Date:10/9'''</big> Sequence(to check mutation of DNA)/The variation was not found<br />
<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max)<br />
:<big>'''Date:9/2'''</big> PCR<br />
:<big>'''Date:9/4'''</big> Transformation<br />
:<big>'''Date:9/5'''</big> Colony PCR<br />
:<big>'''Date:9/7'''</big> ColonyPCR<br />
:<big>'''Date:9/8'''</big> DNA extraction and Sequence(to To confirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/9'''</big> DNA sequence<br />
:<big>'''Date:9/10'''</big> PCR and Electtrophoresis<br />
:<big>'''Date:9/11'''</big> Colony PCR <br />
:<big>'''Date:9/12'''</big> DNA extraction<br />
:<big>'''Date:9/13'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/15'''</big> ultrafiltration<br />
:<big>'''Date:9/16'''</big> DNA sequence(to comfirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/20'''</big> PCR<br />
:<big>'''Date:9/23'''</big> Colony PCR<br />
:<big>'''Date:9/24'''</big> Electrophoresis<br />
:<big>'''Date:9/26'''</big> ultrafiltration<br />
:<big>'''Date:9/27'''</big> PCR and colony PCR<br />
:<big>'''Date:9/29'''</big> Electrophoresis<br />
:<big>'''Date:9/30'''</big> DNA Extraction<br />
:<big>'''Date:10/1'''</big> Colony PCR and DNA extraction<br />
:<big>'''Date:10/2'''</big> Electrophoresis<br />
:<big>'''Date:10/3'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/4'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/5'''</big> Electrophoresis<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
:<big>'''Date:9/16'''</big> PCR:Ara,AraPro,HRV Transformation:IL-5Ra,IL-5Rb<br />
:<big>'''Date:9/17'''</big> Restriction enzyme treatment and insert to TA vector and transformation<br />
:<big>'''Date:9/18'''</big> transformation<br />
:<big>'''Date:9/19'''</big> miniprep and colony PCR<br />
:<big>'''Date:9/20~9/22'''</big> PCR<br />
:<big>'''Date:9/23'''</big> TA Cloning<br />
:<big>'''Date:9/24'''</big> TA Cloning and PCR<br />
:<big>'''Date:9/25'''</big> Restriction enzyme treatment and PCR<br />
:<big>'''Date:9/26'''</big> Colony PCR and Ligation<br />
:<big>'''Date:9/27'''</big> TA Cloning and Ligation and colony PCR<br />
:<big>'''Date:9/28'''</big> PCR<br />
:<big>'''Date:9/29'''</big> colony PCR and PCR and DNA refinement and Ligation<br />
:<big>'''Date:9/30'''</big> DNA refinement and Ligation<br />
:<big>'''Date:10/1'''</big> Restriction enzyme treatment and Ligation<br />
:<big>'''Date:10/2'''</big> PCR and Ligation and DNA refinement <br />
:<big>'''Date:10/3'''</big> Ligation<br />
:<big>'''Date:10/4'''</big> Ligation<br />
:<big>'''Date:10/7'''</big> PCR<br />
:<big>'''Date:10/8'''</big> PCR and Restriction enzyme <br />
:<big>'''Date:10/9'''</big> DNA refinement and Restriction enzyme <br />
:<big>'''Date:10/10'''</big> Phoshatase treatment <br />
:<big>'''Date:10/11'''</big> DNA refinement and Ligation and Restriction enzyme treatment<br />
:<big>'''Date:10/12'''</big> DNA refinement and PCR and Ligation and phoshatase treatment <br />
<br />
<br />
<br />
<br />
<br />
<br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Notebook
Team:KAIT Japan/Notebook
2014-10-17T09:40:13Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
| <br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br><br />
__NOTOC__<br />
<font size ="7">'''Notebook'''</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of GFP and HlyA</font>'''==<br />
:<big>'''Date:8/22'''</big> The refinement of DNA and PCR and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction<br />
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation<br />
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction<br />
:<big>'''Date:8/27'''</big> Electrophoresed the DNA that We did refinement on August 26 to confirmed it<br />
:<big>'''Date:8/28'''</big> Check the HlyA and GFP for colony PCR<br />
:<big>'''Date:8/29'''</big> DNA purify the HlyA and GFP <br />
:<big>'''Date:8/29'''</big> Ligation product and check for PCR<br />
:<big>'''Date:9/1'''</big> Build replica the HlyA and GFP<br />
:<big>'''Date:9/1'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/2'''</big> Restriction enzyme treatment the HlyA and GFP in 9/1 was NANOSEP<br />
:<big>'''Date:9/3'''</big> Ligation and PCR, DNA purification the HlyA and GFP <br />
:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/5'''</big> Restriction enzyme treatment the vector<br />
:<big>'''Date:9/5'''</big> Ligation the vector and insert. So transformation.<br />
:<big>'''Date:9/9'''</big> Check the HiyA and GFP replica.Ligation and transformation the vector and insert DNA.<br />
:<big>'''Date:9/10'''</big> Transformation in 9/9 check the colony PCR.TA cloning the HlyA+GFP.<br />
:<big>'''Date:9/11'''</big> Ligation the vector and insert DNA.<br />
:<big>'''Date:9/12~9/15'''</big> Transformation the Ligation product.<br />
:<big>'''Date:9/16'''</big> Noticed wrong the primer.so restart from the First.<br />
:<big>'''Date:9/17'''</big> PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment.<br />
:<big>'''Date:9/18'''</big> Ligation the GFP and HlyA.<br />
:<big>'''Date:9/19'''</big> Electrophoresis the ligation the GFP+HlyA in 9/18 <br />
:<big>'''Date:9/20'''</big> DNA purify and PCR<br />
:<big>'''Date:9/21'''</big> PCR and DNA purify <br />
:<big>'''Date:9/22'''</big> Ligation the GFP+HlyA<br />
:<big>'''Date:9/23'''</big> PCR and DNA purify<br />
:<big>'''Date:9/24'''</big> TA Cloning was GFP and HlyA<br />
:<big>'''Date:9/26'''</big> Restriction enzyme treatment the ligation the GFP+HlyA in 9/22<br />
:<big>'''Date:9/27'''</big> Check the TA cloning of GFP+HlyA<br />
:<big>'''Date:9/29'''</big> Restriction enzyme treatment the GFP+HlyA and the pSBIC3<br />
:<big>'''Date:9/29'''</big> The vector and the insert were NANOSEP and lectrophoresis<br />
:<big>'''Date:9/30'''</big> The vector and the insert were ligation and transformation<br />
:<big>'''Date:9/30'''</big> The GFP+HlyA was PCR and DNA purify <br />
:<big>'''Date:10/1'''</big> Electrophoresis the GFP+HlyA was PCR and DNA purify in 9/30<br />
:<big>'''Date:10/2'''</big> Restriction enzyme treatment and ligation the GFP+HlyA<br />
:<big>'''Date:10/3'''</big> Check the ligation in 10/2 <br />
:<big>'''Date:10/3'''</big> Transformation the plasmid<br />
:<big>'''Date:10/5'''</big> Colony PCR<br />
:<big>'''Date:10/6'''</big> Miniprep and Electrophoresis<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max) <br />
:<big>'''Date:9/2'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/5~9/8'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/9'''</big> Sequence(to To confirm whether variation happened in DNA of STAT3 )<br />
:<big>'''Date:9/10~9/12'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/13~9/15'''</big> DNA purification and electrophoresis<br />
:<big>'''Date:9/16~9/17'''</big> DNA purification and colony PCR(Failure)<br />
:<big>'''Date:9/18'''</big> colony PCR(Failure)<br />
:<big>'''Date:9/19~9/22'''</big> colony PCR and electrophoresis <br />
:<big>'''Date:9/23'''</big> PCR and Electrophoresis(We found out that DNA polymerase was malfunction)<br />
:<big>'''Date:9/24'''</big> PCR and Electrophoresis and DNA DNA purification <br />
:<big>'''Date:9/25'''</big> PCR(to find annealing temperature)<br />
:<big>'''Date:9/26~9/30'''</big> DNA purification and electrophoresis(to use DNA for the sequence)<br />
:<big>'''Date:10/1'''</big> Dilution after Concentration measurement of DNA<br />
:<big>'''Date:10/2'''</big> Sequence(to check mutation of DNA)/The variation was not found.<br />
:<big>'''Date:10/4'''</big> PCR and electrophoresis <br />
:<big>'''Date:10/5~10/7'''</big> Colony PCR and DNA purification<br />
:<big>'''Date:10/8'''</big> Concentration measurement of DNA<br />
:<big>'''Date:10/9'''</big> Sequence(to check mutation of DNA)/The variation was not found<br />
<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max)<br />
:<big>'''Date:9/2'''</big> PCR<br />
:<big>'''Date:9/4'''</big> Transformation<br />
:<big>'''Date:9/5'''</big> Colony PCR<br />
:<big>'''Date:9/7'''</big> ColonyPCR<br />
:<big>'''Date:9/8'''</big> DNA extraction and Sequence(to To confirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/9'''</big> DNA sequence<br />
:<big>'''Date:9/10'''</big> PCR and Electtrophoresis<br />
:<big>'''Date:9/11'''</big> Colony PCR <br />
:<big>'''Date:9/12'''</big> DNA extraction<br />
:<big>'''Date:9/13'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/15'''</big> ultrafiltration<br />
:<big>'''Date:9/16'''</big> DNA sequence(to comfirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/20'''</big> PCR<br />
:<big>'''Date:9/23'''</big> Colony PCR<br />
:<big>'''Date:9/24'''</big> Electrophoresis<br />
:<big>'''Date:9/26'''</big> ultrafiltration<br />
:<big>'''Date:9/27'''</big> PCR and colony PCR<br />
:<big>'''Date:9/29'''</big> Electrophoresis<br />
:<big>'''Date:9/30'''</big> DNA Extraction<br />
:<big>'''Date:10/1'''</big> Colony PCR and DNA extraction<br />
:<big>'''Date:10/2'''</big> Electrophoresis<br />
:<big>'''Date:10/3'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/4'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/5'''</big> Electrophoresis<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
:<big>'''Date:9/16'''</big> PCR:Ara,AraPro,HRV Transformation:IL-5Ra,IL-5Rb<br />
:<big>'''Date:9/17'''</big> Restriction enzyme treatment and insert to TA vector and transformation<br />
:<big>'''Date:9/18'''</big> transformation<br />
:<big>'''Date:9/19'''</big> miniprep and colony PCR<br />
:<big>'''Date:9/20~9/22'''</big> PCR<br />
:<big>'''Date:9/23'''</big> TA Cloning<br />
:<big>'''Date:9/24'''</big> TA Cloning and PCR<br />
:<big>'''Date:9/25'''</big> Restriction enzyme treatment and PCR<br />
:<big>'''Date:9/26'''</big> Colony PCR and Ligation<br />
:<big>'''Date:9/27'''</big> TA Cloning and Ligation and colony PCR<br />
:<big>'''Date:9/28'''</big> PCR<br />
:<big>'''Date:9/29'''</big> colony PCR and PCR and DNA refinement and Ligation<br />
:<big>'''Date:9/30'''</big> DNA refinement and Ligation<br />
:<big>'''Date:10/1'''</big> Restriction enzyme treatment and Ligation<br />
:<big>'''Date:10/2'''</big> PCR and Ligation and DNA refinement <br />
:<big>'''Date:10/3'''</big> Ligation<br />
:<big>'''Date:10/4'''</big> Ligation<br />
:<big>'''Date:10/7'''</big> PCR<br />
:<big>'''Date:10/8'''</big> PCR and Restriction enzyme <br />
:<big>'''Date:10/9'''</big> DNA refinement and Restriction enzyme <br />
:<big>'''Date:10/10'''</big> Phoshatase treatment <br />
:<big>'''Date:10/11'''</big> DNA refinement and Ligation and Restriction enzyme treatment<br />
:<big>'''Date:10/12'''</big> DNA refinement and PCR and Ligation and phoshatase treatment <br />
<br />
<br />
<br />
<br />
<br />
<br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Notebook
Team:KAIT Japan/Notebook
2014-10-17T09:39:45Z
<p>Iguana: /* Notebook */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
| <br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br><br />
__NOTOC__<br />
<font size ="7">'''Notebook'''</font><br />
:We made an experiment every day. It was process at trial and error.<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of GFP and HlyA</font>'''==<br />
:<big>'''Date:8/22'''</big> The refinement of DNA and PCR and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction<br />
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation<br />
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction<br />
:<big>'''Date:8/27'''</big> Electrophoresed the DNA that We did refinement on August 26 to confirmed it<br />
:<big>'''Date:8/28'''</big> Check the HlyA and GFP for colony PCR<br />
:<big>'''Date:8/29'''</big> DNA purify the HlyA and GFP <br />
:<big>'''Date:8/29'''</big> Ligation product and check for PCR<br />
:<big>'''Date:9/1'''</big> Build replica the HlyA and GFP<br />
:<big>'''Date:9/1'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/2'''</big> Restriction enzyme treatment the HlyA and GFP in 9/1 was NANOSEP<br />
:<big>'''Date:9/3'''</big> Ligation and PCR, DNA purification the HlyA and GFP <br />
:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP<br />
:<big>'''Date:9/5'''</big> Restriction enzyme treatment the vector<br />
:<big>'''Date:9/5'''</big> Ligation the vector and insert. So transformation.<br />
:<big>'''Date:9/9'''</big> Check the HiyA and GFP replica.Ligation and transformation the vector and insert DNA.<br />
:<big>'''Date:9/10'''</big> Transformation in 9/9 check the colony PCR.TA cloning the HlyA+GFP.<br />
:<big>'''Date:9/11'''</big> Ligation the vector and insert DNA.<br />
:<big>'''Date:9/12~9/15'''</big> Transformation the Ligation product.<br />
:<big>'''Date:9/16'''</big> Noticed wrong the primer.so restart from the First.<br />
:<big>'''Date:9/17'''</big> PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment.<br />
:<big>'''Date:9/18'''</big> Ligation the GFP and HlyA.<br />
:<big>'''Date:9/19'''</big> Electrophoresis the ligation the GFP+HlyA in 9/18 <br />
:<big>'''Date:9/20'''</big> DNA purify and PCR<br />
:<big>'''Date:9/21'''</big> PCR and DNA purify <br />
:<big>'''Date:9/22'''</big> Ligation the GFP+HlyA<br />
:<big>'''Date:9/23'''</big> PCR and DNA purify<br />
:<big>'''Date:9/24'''</big> TA Cloning was GFP and HlyA<br />
:<big>'''Date:9/26'''</big> Restriction enzyme treatment the ligation the GFP+HlyA in 9/22<br />
:<big>'''Date:9/27'''</big> Check the TA cloning of GFP+HlyA<br />
:<big>'''Date:9/29'''</big> Restriction enzyme treatment the GFP+HlyA and the pSBIC3<br />
:<big>'''Date:9/29'''</big> The vector and the insert were NANOSEP and lectrophoresis<br />
:<big>'''Date:9/30'''</big> The vector and the insert were ligation and transformation<br />
:<big>'''Date:9/30'''</big> The GFP+HlyA was PCR and DNA purify <br />
:<big>'''Date:10/1'''</big> Electrophoresis the GFP+HlyA was PCR and DNA purify in 9/30<br />
:<big>'''Date:10/2'''</big> Restriction enzyme treatment and ligation the GFP+HlyA<br />
:<big>'''Date:10/3'''</big> Check the ligation in 10/2 <br />
:<big>'''Date:10/3'''</big> Transformation the plasmid<br />
:<big>'''Date:10/5'''</big> Colony PCR<br />
:<big>'''Date:10/6'''</big> Miniprep and Electrophoresis<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max) <br />
:<big>'''Date:9/2'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/5~9/8'''</big> PCR and Electrophoresis <br />
:<big>'''Date:9/9'''</big> Sequence(to To confirm whether variation happened in DNA of STAT3 )<br />
:<big>'''Date:9/10~9/12'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/13~9/15'''</big> DNA purification and electrophoresis<br />
:<big>'''Date:9/16~9/17'''</big> DNA purification and colony PCR(Failure)<br />
:<big>'''Date:9/18'''</big> colony PCR(Failure)<br />
:<big>'''Date:9/19~9/22'''</big> colony PCR and electrophoresis <br />
:<big>'''Date:9/23'''</big> PCR and Electrophoresis(We found out that DNA polymerase was malfunction)<br />
:<big>'''Date:9/24'''</big> PCR and Electrophoresis and DNA DNA purification <br />
:<big>'''Date:9/25'''</big> PCR(to find annealing temperature)<br />
:<big>'''Date:9/26~9/30'''</big> DNA purification and electrophoresis(to use DNA for the sequence)<br />
:<big>'''Date:10/1'''</big> Dilution after Concentration measurement of DNA<br />
:<big>'''Date:10/2'''</big> Sequence(to check mutation of DNA)/The variation was not found.<br />
:<big>'''Date:10/4'''</big> PCR and electrophoresis <br />
:<big>'''Date:10/5~10/7'''</big> Colony PCR and DNA purification<br />
:<big>'''Date:10/8'''</big> Concentration measurement of DNA<br />
:<big>'''Date:10/9'''</big> Sequence(to check mutation of DNA)/The variation was not found<br />
<br />
<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis<br />
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis<br />
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)<br />
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)<br />
:<big>'''Date:8/27'''</big> First Variation introduction (with Prime STAR Max)<br />
:<big>'''Date:9/2'''</big> PCR<br />
:<big>'''Date:9/4'''</big> Transformation<br />
:<big>'''Date:9/5'''</big> Colony PCR<br />
:<big>'''Date:9/7'''</big> ColonyPCR<br />
:<big>'''Date:9/8'''</big> DNA extraction and Sequence(to To confirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/9'''</big> DNA sequence<br />
:<big>'''Date:9/10'''</big> PCR and Electtrophoresis<br />
:<big>'''Date:9/11'''</big> Colony PCR <br />
:<big>'''Date:9/12'''</big> DNA extraction<br />
:<big>'''Date:9/13'''</big> PCR and Electrophoresis<br />
:<big>'''Date:9/15'''</big> ultrafiltration<br />
:<big>'''Date:9/16'''</big> DNA sequence(to comfirm whether variation happened in DNA of IL-10β)<br />
:<big>'''Date:9/20'''</big> PCR<br />
:<big>'''Date:9/23'''</big> Colony PCR<br />
:<big>'''Date:9/24'''</big> Electrophoresis<br />
:<big>'''Date:9/26'''</big> ultrafiltration<br />
:<big>'''Date:9/27'''</big> PCR and colony PCR<br />
:<big>'''Date:9/29'''</big> Electrophoresis<br />
:<big>'''Date:9/30'''</big> DNA Extraction<br />
:<big>'''Date:10/1'''</big> Colony PCR and DNA extraction<br />
:<big>'''Date:10/2'''</big> Electrophoresis<br />
:<big>'''Date:10/3'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/4'''</big> PCR(using Prime Star MAX Premix) and Electrophoresis<br />
:<big>'''Date:10/5'''</big> Electrophoresis<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
:<big>'''Date:9/16'''</big> PCR:Ara,AraPro,HRV Transformation:IL-5Ra,IL-5Rb<br />
:<big>'''Date:9/17'''</big> Restriction enzyme treatment and insert to TA vector and transformation<br />
:<big>'''Date:9/18'''</big> transformation<br />
:<big>'''Date:9/19'''</big> miniprep and colony PCR<br />
:<big>'''Date:9/20~9/22'''</big> PCR<br />
:<big>'''Date:9/23'''</big> TA Cloning<br />
:<big>'''Date:9/24'''</big> TA Cloning and PCR<br />
:<big>'''Date:9/25'''</big> Restriction enzyme treatment and PCR<br />
:<big>'''Date:9/26'''</big> Colony PCR and Ligation<br />
:<big>'''Date:9/27'''</big> TA Cloning and Ligation and colony PCR<br />
:<big>'''Date:9/28'''</big> PCR<br />
:<big>'''Date:9/29'''</big> colony PCR and PCR and DNA refinement and Ligation<br />
:<big>'''Date:9/30'''</big> DNA refinement and Ligation<br />
:<big>'''Date:10/1'''</big> Restriction enzyme treatment and Ligation<br />
:<big>'''Date:10/2'''</big> PCR and Ligation and DNA refinement <br />
:<big>'''Date:10/3'''</big> Ligation<br />
:<big>'''Date:10/4'''</big> Ligation<br />
:<big>'''Date:10/7'''</big> PCR<br />
:<big>'''Date:10/8'''</big> PCR and Restriction enzyme <br />
:<big>'''Date:10/9'''</big> DNA refinement and Restriction enzyme <br />
:<big>'''Date:10/10'''</big> Phoshatase treatment <br />
:<big>'''Date:10/11'''</big> DNA refinement and Ligation and Restriction enzyme treatment<br />
:<big>'''Date:10/12'''</big> DNA refinement and PCR and Ligation and phoshatase treatment <br />
<br />
<br />
<br />
<br />
<br />
<br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:38:29Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4"><br />
STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of IL-10α,IL-10β</H1><br />
<font size="4"><br />
IL-10a's sequence includes six,IL-10b's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant these sequences ,but we didn't succeed.<br />
</font><br />
<br><br />
<br><br />
<br />
<br />
<br />
<br />
<H1>Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:27:04Z
<p>Iguana: /* Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4">STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of IL-10α,IL-10β</H1><br />
<font size="4"><br />
<br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:26:39Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4">STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of IL-10α,IL-10β</H1><br />
<font size="4"><br />
<br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:25:54Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4">STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
<H1>Creating parts of IL-10α,IL-10β<H1><br />
<font size="4"><br />
<br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:22:36Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
<H1>GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</H1><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
<H1>Creating parts of STAT3</H1><br />
<font size="4">STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<font size="4"><br />
<br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:17:07Z
<p>Iguana: /* Creating parts of IL-10α,IL-10β */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<font size="4">STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<font size="4"><br />
<br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:14:20Z
<p>Iguana: /* Creating parts of STAT3 */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<font size="4">STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:14:00Z
<p>Iguana: /* Creating parts of STAT3 */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<font size="4>"STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:13:47Z
<p>Iguana: /* Creating parts of STAT3 */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
,font size="4>"STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
</font><br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:13:08Z
<p>Iguana: /* Creating parts of STAT3 */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
STAT3's sequence includes three recognition sequences of restriction enzyme.<br />
We try to mutant this sequence,and we could succeed to mutant about one place. <br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:04:13Z
<p>Iguana: /* Colony PCR of parts */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"> Result of colony PCR </font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:03:05Z
<p>Iguana: /* */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">Colony PCR of parts</font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:02:23Z
<p>Iguana: /* GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5"></font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T09:01:52Z
<p>Iguana: /* GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
Figure 2 is colony PCR of colony transformed TA vector inserted AraC gene.<br />
The band of lane 1,2 were 1104bp.<br />
Objective band size was 1149bp.<br />
We are able to confirm that we get colony which has TA vector inserted AraC gene.<br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:56:45Z
<p>Iguana: /* GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
Figure 1 is colony PCR of colony transformed TA vector.<br />
Lane 1,2 are colony transformed TA vector inserted AraC gene.<br />
Lane 3,4 are colony transformed TA vector inserted AraC promoter gene.<br />
Lane 5,6,7 are colony transformed TA vector inserted HRV gene.<br />
The band of lane 5 was 325bp.<br />
Objective band size was 325bp.<br />
We are able to confirm that we get colony which has TA vecter inserted AraC promoter gene.<br />
<br><br />
<br><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:47:49Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:140929 araC araC promoter TAvector colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/File:140929_araC_araC_promoter_TAvector_colonyPCR.jpg
File:140929 araC araC promoter TAvector colonyPCR.jpg
2014-10-17T08:47:20Z
<p>Iguana: </p>
<hr />
<div></div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:47:05Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:140927 Arac AracP HRV colonyPCR.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/File:140927_Arac_AracP_HRV_colonyPCR.jpg
File:140927 Arac AracP HRV colonyPCR.jpg
2014-10-17T08:46:39Z
<p>Iguana: </p>
<hr />
<div></div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:42:07Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP.<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:41:45Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:41:19Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br />
Following figure 3 is GFP+HlyA.<br />
Following figure 4 is HlyA+GFP<br />
We submitted these parts.<br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:41:07Z
<p>Iguana: /* HlyA */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br />
Length of "BBa_K554002" is 186bp.<br />
Following figure 2 is result of the HlyA PCR.<br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is GFP+HlyA.<br><br />
Following figure 4 is HlyA+GFP<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:40:55Z
<p>Iguana: /* GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br />
Length of "BBa_E0040"is 920bp.<br />
Following figure 1 is result of the GFP PCR.<br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is GFP+HlyA.<br><br />
Following figure 4 is HlyA+GFP<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:40:28Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is GFP+HlyA.<br><br />
Following figure 4 is HlyA+GFP<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:40:04Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is GFP+HlyA.Following figure 4 is HlyA+GFP<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:38:45Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is it.<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==<br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
[[File:Example.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
<br />
</font><br />
|}</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:35:41Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is it.<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:35:21Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.Length of GFP+HlyA is 920bp<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:34:20Z
<p>Iguana: /* HlyA */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
Length of "BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:34:08Z
<p>Iguana: /* GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
Length of "BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
"BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:32:25Z
<p>Iguana: /* GFP+HlyA and HlyA+GFP */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
"BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
"BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.<br><br />
But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:32:00Z
<p>Iguana: /* HlyA */</p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
"BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".<br><br />
"BBa_K554002" is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:29:41Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="5">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
"BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".This's molucular is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana
http://2014.igem.org/Team:KAIT_Japan/Results
Team:KAIT Japan/Results
2014-10-17T08:28:40Z
<p>Iguana: </p>
<hr />
<div>[[File:ロゴ2.png|left|link=https://igem.org/Team.cgi]]<br />
[[File:KAIT Japan2013 Kanako.png|link=http://www.kait.jp/index2.php]]<br />
[[File:KAIT Japan 2014 iGEMRogo.png|center|link=https://2014.igem.org/Main_Page]]<br />
{|style= width="100%" align="center"<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New home t.jpg|link=Team:KAIT JAPAN]]<br />
[[Team:KAIT JAPAN|Home]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New Team1.jpg|link=Team:KAIT_Japan/Team]]<br />
[[Team:KAIT_Japan/Team|Team]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 考える人.jpg|link=Team:KAIT_Japan/Project]]<br />
[[Team:KAIT_Japan/Project|Project]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 歯車.jpg|link=Team:KAIT_Japan/Parts]]<br />
[[Team:KAIT_Japan/Parts|Parts]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 111111111111111.jpg|link=Team:KAIT_Japan/Protocol]]<br />
[[Team:KAIT_Japan/Protocol|Protocol]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:Ff.jpg|link=Team:KAIT_Japan/Notebook]]<br />
[[Team:KAIT_Japan/Notebook|Notebook]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New 結果.jpg|link=Team:KAIT_Japan/Results]]<br />
[[Team:KAIT_Japan/Results|Results]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new 危険.jpg|link=Team:KAIT_Japan/Safety]]<br />
[[Team:KAIT_Japan/Safety|Safety]]<br />
|}<br />
|<br />
{|style="background:#FFFFFF; margin:0.1em"<br />
!align="center"|[[File:New new ひと.jpg|link=Team:KAIT_Japan/Human_Practice]]<br />
[[Team:KAIT_Japan/Human_Practice|Human Practice]]<br />
|}<br />
|<br />
|}<br />
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ffffe0; text-align:left"<br />
| style="width:30% style="vertical-align:top" |<br />
<br />
<H1>Results</H1><br />
<br><br />
=='''<font size="6">GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)</font>'''==<br />
<font size=4> </font><br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:GFP.jpg]]<br />
::::::<font size=4> [Figure 1]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP</font>'''==<br />
<font size=4><br />
We used "BBa_E0040".<br><br />
"BBa_E0040"is 920bp.<br><br />
Following figure 1 is result of the GFP PCR.<br><br />
As a result,We were able to confirm a band about 920bp by GFP PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 2]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">HlyA</font>'''==<br />
<font size=4><br />
We used "BBa_K554002".This's molucular is 186bp.<br><br />
Following figure 2 is result of the HlyA PCR.<br><br />
As a result,We were able to confirm a band about 186bp by HlyA PCR. <br />
</font><br />
|}<br />
<br><br />
<br><br />
<br />
{| align="left" style="width:100%; margin-right:0.2em; border-color:#99F; border-style:dotted; border-collapse:collapse; background-color:#FFF;"<br />
| style="width:150px" |<br />
[[File:HlyA.jpg]]<br />
::::::<font size=4> [Figure 3]</font><br />
[[File:GFP+HlyA.jpg]]<br />
::::::<font size=4> [Figure 4]</font><br />
| style="border-left:3px dotted #99F" |<br />
<br />
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==<br />
<font size=4><br />
GFP ligated with HlyA.<br><br />
Following figure 3 is GFP+HlyA.<br><br />
We submitted these parts.But the function of these parts,we were not able to confirm it.<br><br />
Because we did not have time to experiment.<br />
<br />
</font><br />
|}<br />
<br />
=='''<font size="6">Creating parts of STAT3</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==<br />
<br />
<br><br />
<br><br />
<br />
=='''<font size="6">Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter</font>'''==</div>
Iguana