http://2014.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=20&target=Kariny8882014.igem.org - User contributions [en]2024-03-28T08:58:09ZFrom 2014.igem.orgMediaWiki 1.16.5http://2014.igem.org/File:Bio-X_Logo.pngFile:Bio-X Logo.png2015-07-28T12:25:44Z<p>Kariny888: uploaded a new version of &quot;File:Bio-X Logo.png&quot;</p>
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<div></div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/ProjectTeam:SJTU-BioX-Shanghai/Project2015-07-08T09:20:58Z<p>Kariny888: </p>
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<center><h2>Enzymes, in the name of crown, get closer!</h2></center> </div><br />
<div class="projtile_img"><center><img weight="666.67px" height="400px" src="https://static.igem.org/mediawiki/2014/f/ff/SJTU14_Overview.png"></img></center><br />
<p>This year, our project intends to achieve protein polymerization with the help of TAL effector (Tale Transcription Activator–like Effector), a DNA-binding protein, which can recognize a specific nucleotide sequence. With circular DNA(plasmid) as the connection medium, expressed proteins in the host can form a complex selectively, so that a variety of enzyme combinations can be used to complete different tasks. Compared to traditional methods, the advantages of the project lie in selective polymerization of proteins and the ability to mediate polymerization between more proteins..</p></div><br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part1_Connect" title="Connect"><br />
<center><h2>Connection</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/9/96/SJTU14_connect_small.png"></img></center></a><br />
<p>Want to know more about how to build the crown?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
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<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part2_Extension" title="Maximize"><br />
<center><h2>Polymerization</h2></center><br/><br />
<center><img src="/wiki/images/5/51/SJTU14_Application_small.png"></img></center></a><br />
<p>What makes this little crown a real useful tool?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
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<a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement" title="Gear Box"><br />
<center><h2>TAL Improvement</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/f/f9/SJTU14_TAL_small.png"></img></a></center><br />
<p>We work for the better using of TAL!<br/><a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement">Click here~</a></p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/ProjectTeam:SJTU-BioX-Shanghai/Project2015-07-08T09:20:18Z<p>Kariny888: </p>
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<center><h2>Enzymes, in the name of crown, get closer!</h2></center> </div><br />
<div class="projtile_img"><center><img weight="666.67px" height="400px" src="https://static.igem.org/mediawiki/2014/f/ff/SJTU14_Overview.png"></img></center><br />
<p>This year, our project intends to achieve protein polymerization with the help of TAL effector (Tale Transcription Activator–like Effector), a DNA-binding protein, which can recognize a specific nucleotide sequence. With circular DNA(plasmid) as the connection medium, expressed proteins in the host can form a complex selectively, so that a variety of enzyme combinations can be used to complete different tasks. Compared to traditional methods, the advantages of the project lie in selective polymerization of proteins and the ability to mediate polymerization between more proteins..</p></div><br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part1_Connect" title="Connect"><br />
<center><h2>Connection</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/9/96/SJTU14_connect_small.png"></img></center></a><br />
<p>Want to know more about how to build the crown?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
<br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part2_Extension" title="Maximize"><br />
<center><h2>Polymerization</h2></center><br/><br />
<center><img src="/wiki/images/5/51/SJTU14_Application_small.png"></img></center></a><br />
<p>What makes this little crown a real useful tool?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
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<div class="projtile"><br />
<a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement" title="Gear Box"><br />
<center><h2>TAL Improvement</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/f/f9/SJTU14_TAL_small.png"></img></a></center><br />
<p>We work for the better using of TAL!<br/><a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement">Click here~</a></p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/ProjectTeam:SJTU-BioX-Shanghai/Project2015-07-08T09:19:24Z<p>Kariny888: </p>
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<center><h2>Enzymes, in the name of crown, get closer!</h2></center> </div><br />
<div class="projtile_img"><center><img weight="666.67px" height="400px" src="https://static.igem.org/mediawiki/2014/f/ff/SJTU14_Overview.png"></img></center><br />
<p>This year, our project intends to achieve protein polymerization with the help of TAL effector (Tale Transcription Activator–like Effector), a DNA-binding protein, which can recognize a specific nucleotide sequence. With circular DNA(plasmid) as the connection medium, expressed proteins in the host can form a complex selectively, so that a variety of enzyme combinations can be used to complete different tasks. Compared to traditional methods, the advantages of the project lie in selective polymerization of proteins and the ability to mediate polymerization between more proteins..</p></div><br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part1_Connect" title="Connect"><br />
<center><h2>Connection</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/9/96/SJTU14_connect_small.png"></img></center></a><br />
<p>Want to know more about how to build the crown?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
<br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part2_Extension" title="Maximize"><br />
<center><h2>Polymerization</h2></center><br/><br />
<center><img src="/wiki/images/5/51/SJTU14_Application_small.png"></img></center></a><br />
<p>What makes this little crown a real useful tool?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
<br />
<br />
<div class="projtile"><br />
<a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement" title="Gear Box"><br />
<center><h2>TAL Improvement</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/f/f9/SJTU14_TAL_small.png"></img></a></center><br />
<p>We work for the better using of TAL!<br/><a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement">Click here~</a></p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/ProjectTeam:SJTU-BioX-Shanghai/Project2015-07-08T09:18:50Z<p>Kariny888: </p>
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<br />
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<center><h2>Enzymes, in the name of crown, get closer!</h2></center> </div><br />
<div class="projtile_img"><center><img weight="666.67px" height="400px" src="https://static.igem.org/mediawiki/2014/f/ff/SJTU14_Overview.png"></img></center><br />
<p>This year, our project intends to achieve protein polymerization with the help of TAL effector (Tale Transcription Activator–like Effector), a DNA-binding protein, which can recognize a specific nucleotide sequence. With circular DNA(plasmid) as the connection medium, expressed proteins in the host can form a complex selectively, so that a variety of enzyme combinations can be used to complete different tasks. Compared to traditional methods, the advantages of the project lie in selective polymerization of proteins and the ability to mediate polymerization between more proteins..</p></div><br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part1_Connect" title="Connect"><br />
<center><h2>Connection</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/9/96/SJTU14_connect_small.png"></img></center></a><br />
<p>Want to know more about how to build the crown?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
<br />
<div class="projtile"><br />
<a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Part2_Extension" title="Maximize"><br />
<center><h2>Polymerization</h2></center><br/><br />
<center><img src="/wiki/images/5/51/SJTU14_Application_small.png"></img></center></a><br />
<p>What makes this little crown a real useful tool?<br/><a href="/Team:SJTU-BioX-Shanghai/Part2_Extension">Click here~</a></p><br />
</div><br />
<br />
<br />
<div class="projtile"><br />
<a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement" title="Gear Box"><br />
<center><h2>TAL Improvement</h2></center><br/><br />
<center><img src="https://static.igem.org/mediawiki/2014/f/f9/SJTU14_TAL_small.png"></img></a></center><br />
<p>We work for the better using of TAL!<br/><a href="/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement">Click here~</a></p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Template:Team:SJTU-BioX-Shanghai/Front_HeaderTemplate:Team:SJTU-BioX-Shanghai/Front Header2015-07-06T06:26:26Z<p>Kariny888: Undo revision 406696 by Kariny888 (talk)</p>
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</html></div>Kariny888http://2014.igem.org/Template:Team:SJTU-BioX-Shanghai/Front_HeaderTemplate:Team:SJTU-BioX-Shanghai/Front Header2015-07-06T06:25:38Z<p>Kariny888: </p>
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</html></div>Kariny888http://2014.igem.org/Template:Team:SJTU-BioX-Shanghai/previewTemplate:Team:SJTU-BioX-Shanghai/preview2014-10-17T22:35:24Z<p>Kariny888: </p>
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</html></div>Kariny888http://2014.igem.org/Template:Team:SJTU-BioX-Shanghai/previewTemplate:Team:SJTU-BioX-Shanghai/preview2014-10-17T22:31:06Z<p>Kariny888: </p>
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Week Notes<br />
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<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#July">July</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#August">August</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#September">September</a></p><br />
</div><br />
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<div class="sidenav"><br />
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Protocol<br />
</div><br />
<div class="content"><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Molecule">Molecule</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Cell">Cell</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Protein">Protein</a></p><br />
</div><br />
</div><br />
<br />
</div><br />
<div style="padding-left: 15%; background-color: #fff;"><br />
<div class="content"><br />
<article class="post__article"><br />
<h3 id="July">July Week 1: Plasmid Amplification</h3><br><br />
<p>We chose pRSFDuet-1, pACYCDuet-1 and pCDFDuet-1 as expression vectors and pBluescript II KS(+) as the connector. These plasmids were amplified for further construction.<p><br />
<img src="https://static.igem.org/mediawiki/2014/0/07/SJTU14-notebook-week1.jpg"width=50% height=50%><br />
<br />
<h3 id ="JulyWeek2" >July Week 2: Plan Making</h3><br><br />
<p>We intended to construct the gene of our fusion protein, ssDsbA-mRFP-HL-Lgt-FL-TAL-His Tag, using overlap PCR, enzyme digestion and ligation. After careful consideration, we decided to connect ssDsbA-mRFP-HL-Lgt as one part of this fusion protein and FL-TAL-His Tag for another, so that they could be connected together.<p><br />
<br />
<h3 id ="JulyWeek3" >July Week 3: Construction of Part 1</h3><br><br />
<p> We constructed the first part, ssDsbA-mRFP-HL-Lgt with overlap PCR and ligated it into the pBluescript II KS(+). Then we obtained more plasmids through transformation, colony picking and plasmid extraction. After that, we verified them with digestion identification and sequencing. Sequencing results showed accurate construction.<p><br />
<img src="https://static.igem.org/mediawiki/2014/8/82/SJTU14-July_week3.jpg"width=50% height=50%><br />
<p><img src="https://static.igem.org/mediawiki/2014/8/83/SJTU14-July_week4.jpg"width=50% height=50%><br />
<br />
<h3 id ="JulyWeek4" >July Week 4: TAL Connection</h3><br><br />
<p> In order to construct the second part, we had to obtain the TAL we needed using bioparts from 2012 Freiburg iGEM team. But unfortunately, we didn't get any positive result.<p><br />
<img src="https://static.igem.org/mediawiki/2014/3/32/SJTU14-week4-1.jpg"width=10% height=10%><br />
<img src="https://static.igem.org/mediawiki/2014/a/a3/SJTU14-week4-2.jpg"width=25% height=25%><br />
<br />
<h3 id="August">August Week 1-2: PCR Optimization</h3><br><br />
<p>Because of the negative results, we decided to adjust some PCR parameters, including the annealing temperature, template concentration and cycle number. Test the conditions for the PCR. <br />
Connected TAL, transform, colony picking plasmid extraction and digestion identification. Find with our electrophoresis band. Expression vectors and connector plasmid are confirmed by sequencing.<p><br />
<img src="https://static.igem.org/mediawiki/2014/f/fb/SJTU14-August_week1~2-1.jpg"width=25% height=25%><br />
<img src="https://static.igem.org/mediawiki/2014/1/11/SJTU14-August_week1~2-2.jpg"width=35% height=35%><br />
<br />
<h3 id ="AugustWeek3" >August Week3:</h3><br><br />
<p>There are some problems about Freiburg’s parts. We can’t connect TAL in the right order. <br />
So we design some new primers for PCR that can produce the right sequence.<p><br />
<br />
<h3 id ="AugustWeek4" >August Week4</h3><br><br />
<p>Design a few new ports for the fusion protein. <br />
Sequencing results showed accurate construction. Observe the FP using LSCM to confirm the fusion protein can locate on the membrane.<p> <br />
<br />
<h3 id="September" >September Week1</h3><br><br />
<p>Try co-transformation: Prsf pacyc pBluescript . <br />
Find the conditions of protein expression. <br />
Find the way to construct the TAL.<br />
Start to synthesis the TAL gene <p> <br />
<br />
<h3 id ="SeptemberWeek2" >September Week2</h3><br><br />
<p>Find the enzymes for the application. <br />
Find the way to detect the substrate in these pathways. <br />
Connector plasmid modification.<p> <br />
<p>Start to design the primers used in the vector remoulding.<br />
<br />
<h3 id ="SeptemberWeek3" >September Week3</h3><br><br />
<p>The synthesized TAL gene sequence from a gene company received; continue to construct the part with our new ports.<p> <br />
<p>remould the PSK vector in order to achieve our aims.<br />
<br />
<h3 id ="SeptemberWeek4" >September Week4</h3><br><br />
<p>Express the TAL gene;Do the test for prokaryotic expression.Express the constructed gene and get the final results.<p> <br />
<br />
</article><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotesTeam:SJTU-BioX-Shanghai/daynotes2014-10-17T22:26:21Z<p>Kariny888: </p>
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Week Notes<br />
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<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#July">July</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#August">August</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#September">September</a></p><br />
</div><br />
<br />
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<div class="sidenav"><br />
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Protocol<br />
</div><br />
<div class="content"><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Molecule">Molecule</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Cell">Cell</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Protein">Protein</a></p><br />
</div><br />
</div><br />
<br />
</div><br />
<div style="padding-left: 15%; background-color: #fff;"><br />
<div class="content"><br />
<article class="post__article"><br />
<h3 id="July">July Week 1: Plasmid Amplification</h3><br><br />
<p>We chose pRSFDuet-1, pACYCDuet-1 and pCDFDuet-1 as expression vectors and pBluescript II KS(+) as the connector. These plasmids were amplified for further construction.<p><br />
<img src="https://static.igem.org/mediawiki/2014/0/07/SJTU14-notebook-week1.jpg"width=50% height=50%><br />
<br />
<h3 id ="JulyWeek2" >July Week 2: Plan Making</h3><br><br />
<p>We intended to construct the gene of our fusion protein, ssDsbA-mRFP-HL-Lgt-FL-TAL-His Tag, using overlap PCR, enzyme digestion and ligation. After careful consideration, we decided to connect ssDsbA-mRFP-HL-Lgt as one part of this fusion protein and FL-TAL-His Tag for another, so that they could be connected together.<p><br />
<br />
<h3 id ="JulyWeek3" >July Week 3: Construction of Part 1</h3><br><br />
<p> We constructed the first part, ssDsbA-mRFP-HL-Lgt with overlap PCR and ligated it into the pBluescript II KS(+). Then we obtained more plasmids through transformation, colony picking and plasmid extraction. After that, we verified them with digestion identification and sequencing. Sequencing results showed accurate construction.<p><br />
<img src="https://static.igem.org/mediawiki/2014/8/82/SJTU14-July_week3.jpg"width=50% height=50%><br />
<p><img src="https://static.igem.org/mediawiki/2014/8/83/SJTU14-July_week4.jpg"width=50% height=50%><br />
<br />
<h3 id ="JulyWeek4" >July Week 4: TAL Connection</h3><br><br />
<p> In order to construct the second part, we had to obtain the TAL we needed using bioparts from 2012 Freiburg iGEM team. But unfortunately, we didn't get any positive result.<p><br />
<img src="https://static.igem.org/mediawiki/2014/3/32/SJTU14-week4-1.jpg"width=10% height=10%><br />
<img src="https://static.igem.org/mediawiki/2014/a/a3/SJTU14-week4-2.jpg"width=25% height=25%><br />
<br />
<h3 id="August">August Week 1-2: PCR Optimization</h3><br><br />
<p>Because of the negative results, we decided to adjust some PCR parameters, including the annealing temperature, template concentration and cycle number. Test the conditions for the PCR. <br />
Connected TAL, transform, colony picking plasmid extraction and digestion identification. Find with our electrophoresis band. Expression vectors and connector plasmid are confirmed by sequencing.<p><br />
<img src="https://static.igem.org/mediawiki/2014/f/fb/SJTU14-August_week1~2-1.jpg"width=25% height=25%><br />
<img src="https://static.igem.org/mediawiki/2014/1/11/SJTU14-August_week1~2-2.jpg"width=35% height=35%><br />
<br />
<h3 id ="AugustWeek3" >August Week3:</h3><br><br />
<p>There are some problems about Freiburg’s parts. We can’t connect TAL in the right order. <br />
So we design some new primers for PCR that can produce the right sequence.<p><br />
<br />
<h3 id ="AugustWeek4" >August Week4</h3><br><br />
<p>Design a few new ports for the fusion protein. <br />
Sequencing results showed accurate construction. Observe the FP using LSCM to confirm the fusion protein can locate on the membrane.<p> <br />
<br />
<h3 id="September" >September Week1</h3><br><br />
<p>Try co-transformation: Prsf pacyc pBluescript . <br />
Find the conditions of protein expression. <br />
Find the way to construct the TAL.<br />
Start to synthesis the TAL gene <p> <br />
<br />
<h3 id ="SeptemberWeek2" >September Week2</h3><br><br />
<p>Find the enzymes for the application. <br />
Find the way to detect the substrate in these pathways. <br />
Connector plasmid modification.<p> <br />
<p>Start to design the primers used in the vector remoulding.<br />
<br />
<h3 id ="SeptemberWeek3" >September Week3</h3><br><br />
<p>The synthesized TAL gene sequence from a gene company received; continue to construct the part with our new ports.<p> <br />
<p>remould the PSK vector in order to achieve our aims.<br />
<br />
<h3 id ="SeptemberWeek4" >September Week4</h3><br><br />
<p>Express the TAL gene;Do the test for prokaryotic expression.Express the constructed gene and get the final results.<p> <br />
<br />
</article><br />
</div><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotesTeam:SJTU-BioX-Shanghai/daynotes2014-10-17T22:25:53Z<p>Kariny888: </p>
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Week Notes<br />
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<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#July">July</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#August">August</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/daynotes#September">September</a></p><br />
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Protocol<br />
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<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Molecule">Molecule</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Cell">Cell</a></p><br />
<p><a href="https://2014.igem.org/Team:SJTU-BioX-Shanghai/Protocol#Protein">Protein</a></p><br />
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<h3 id="July">July Week 1: Plasmid Amplification</h3><br><br />
<p>We chose pRSFDuet-1, pACYCDuet-1 and pCDFDuet-1 as expression vectors and pBluescript II KS(+) as the connector. These plasmids were amplified for further construction.<p><br />
<img src="https://static.igem.org/mediawiki/2014/0/07/SJTU14-notebook-week1.jpg"width=50% height=50%><br />
<br />
<h3 id ="JulyWeek2" >July Week 2: Plan Making</h3><br><br />
<p>We intended to construct the gene of our fusion protein, ssDsbA-mRFP-HL-Lgt-FL-TAL-His Tag, using overlap PCR, enzyme digestion and ligation. After careful consideration, we decided to connect ssDsbA-mRFP-HL-Lgt as one part of this fusion protein and FL-TAL-His Tag for another, so that they could be connected together.<p><br />
<br />
<h3 id ="JulyWeek3" >July Week 3: Construction of Part 1</h3><br><br />
<p> We constructed the first part, ssDsbA-mRFP-HL-Lgt with overlap PCR and ligated it into the pBluescript II KS(+). Then we obtained more plasmids through transformation, colony picking and plasmid extraction. After that, we verified them with digestion identification and sequencing. Sequencing results showed accurate construction.<p><br />
<img src="https://static.igem.org/mediawiki/2014/8/82/SJTU14-July_week3.jpg"width=50% height=50%><br />
<p><img src="https://static.igem.org/mediawiki/2014/8/83/SJTU14-July_week4.jpg"width=50% height=50%><br />
<br />
<h3 id ="JulyWeek4" >July Week 4: TAL Connection</h3><br><br />
<p> In order to construct the second part, we had to obtain the TAL we needed using bioparts from 2012 Freiburg iGEM team. But unfortunately, we didn't get any positive result.<p><br />
<img src="https://static.igem.org/mediawiki/2014/3/32/SJTU14-week4-1.jpg"width=10% height=10%><br />
<img src="https://static.igem.org/mediawiki/2014/a/a3/SJTU14-week4-2.jpg"width=25% height=25%><br />
<br />
<h3 id="August">August Week 1-2: PCR Optimization</h3><br><br />
<p>Because of the negative results, we decided to adjust some PCR parameters, including the annealing temperature, template concentration and cycle number. Test the conditions for the PCR. <br />
Connected TAL, transform, colony picking plasmid extraction and digestion identification. Find with our electrophoresis band. Expression vectors and connector plasmid are confirmed by sequencing.<p><br />
<img src="https://static.igem.org/mediawiki/2014/f/fb/SJTU14-August_week1~2-1.jpg"width=25% height=25%><br />
<img src="https://static.igem.org/mediawiki/2014/1/11/SJTU14-August_week1~2-2.jpg"width=35% height=35%><br />
<br />
<h3 id ="AugustWeek3" >August Week3:</h3><br><br />
<p>There are some problems about Freiburg’s parts. We can’t connect TAL in the right order. <br />
So we design some new primers for PCR that can produce the right sequence.<p><br />
<br />
<h3 id ="AugustWeek4" >August Week4</h3><br><br />
<p>Design a few new ports for the fusion protein. <br />
Sequencing results showed accurate construction. Observe the FP using LSCM to confirm the fusion protein can locate on the membrane.<p> <br />
<br />
<h3 id="September" >September Week1</h3><br><br />
<p>Try co-transformation: Prsf pacyc pBluescript . <br />
Find the conditions of protein expression. <br />
Find the way to construct the TAL.<br />
Start to synthesis the TAL gene <p> <br />
<br />
<h3 id ="SeptemberWeek2" >September Week2</h3><br><br />
<p>Find the enzymes for the application. <br />
Find the way to detect the substrate in these pathways. <br />
Connector plasmid modification.<p> <br />
<p>Start to design the primers used in the vector remoulding.<br />
<br />
<h3 id ="SeptemberWeek3" >September Week3</h3><br><br />
<p>The synthesized TAL gene sequence from a gene company received; continue to construct the part with our new ports.<p> <br />
<p>remould the PSK vector in order to achieve our aims.<br />
<br />
<h3 id ="SeptemberWeek4" >September Week4</h3><br><br />
<p>Express the TAL gene;Do the test for prokaryotic expression.Express the constructed gene and get the final results.<p> <br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T22:02:45Z<p>Kariny888: </p>
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<h2>Thanks for all the effort</h2><br />
<p>We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/1/18/SJTU14_sjtu-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img style="width:300px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T22:01:49Z<p>Kariny888: </p>
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<p><br />
We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/1/18/SJTU14_sjtu-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img style="width:300px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T22:01:27Z<p>Kariny888: </p>
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<p><br />
We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/1/18/SJTU14_sjtu-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img style="width:300px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T22:00:20Z<p>Kariny888: </p>
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<p><br />
We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/1/18/SJTU14_sjtu-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img style="width:300px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T21:59:57Z<p>Kariny888: </p>
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We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/1/18/SJTU14_sjtu-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img style="width:300px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T21:57:27Z<p>Kariny888: </p>
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<p><br />
We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img style="width:300px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T21:57:01Z<p>Kariny888: </p>
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<p><br />
We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/c/cf/SJTU14_life-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img src=style="width:300px;" "/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888http://2014.igem.org/Team:SJTU-BioX-Shanghai/AttributionsTeam:SJTU-BioX-Shanghai/Attributions2014-10-17T21:56:28Z<p>Kariny888: </p>
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<article><br />
<p><br />
We sincerely thank for all the help given by the research institute of Bio-X. During the whole preparation procedure of the igem competition, Bio-X offered us a great support in all aspects. <img src="/wiki/images/5/51/SJTU14_bio-x-ing.png"></img>Thanks to their providing of experimental funds and drugs. Thanks to our supervisor Gang Ma, the professor of Bio-X who supported us with a good experimental condition. Thanks to our instructor Yushu Wang, who spent a lot of time and energy on us, we fought together dealing with all of the hard problems. Without their help and encouragement, we can not held up to the end. And we also give thanks to all other teachers and fellow students who supported us in either experiment or brainstorming in Bio-X. Our whole project is mostly based on their understanding and help!<br />
</p><br />
<img style="float:right;width:200px;" src="/wiki/images/1/18/SJTU14_sjtu-ing.jpg"></img><p>We sincerely thank for all the help given by Sbanghai Jiao Tong University. Thanks for the substantial support given by our university. Our university gives us logistics support for our whole project.</p><br />
<p>We sincerely thank for all the help given by School of Life Sciences and Biotechnology.<img src=style="width:300px;" "/wiki/images/c/cf/SJTU14_life-ing.jpg"></img> During the whole experimentation, School of Life Science and Biotechnology provided us with experimental funds, laboratory and experimental apparatus. We also thank for all the professors who helped us dealing with difficulties in SLSB.</p><br />
<br></br><br />
<p><strong>Binhan Hao</strong> Primarily in charge of wet lab. Designed Enzyme USB when we need insert target enzyme into our part. Moreover, the most valuable work is that he found out the sticky ends problem with 2012 Freiburg’ project and wrote down a article about the problem.</p><br />
<p><strong>Renhe Luo</strong> Responsible for almost everything in wet lab.</p><br />
<p><strong>Ziyang Tan</strong> Remould the vectors of pBluescript II KS(+)_3_copy and pBluescript II KS(+)_5_copy. Assist in constructing the main part.</p><br />
<p><strong>Yangyang Li</strong> Mainly in charge of enzyme polymerization verification and help with TAL construction as well.</p><br />
<p><strong>Xiangdong Bu</strong> Take part in project designing, do some experiments in wet lab and do a few jobs in dry lab.</p><br />
<p><strong>Qiao Zhou</strong> Responsible for TAL ligation ,protein expression and inverse PCR</p><br />
<p><strong>Ming Chen</strong> Took part in the wet lab part of our project and sometimes helped with the dry lab.</p><br />
<p><strong>Yaojin Sun</strong> Responsible for modeling parts.</p><br />
<p><strong>Mingzhao Chen</strong> Responsible for modeling parts.</p><br />
<p><strong>Yitian Yao</strong> Responsible for modeling parts.</p><br />
<p><strong>Kang Ren</strong> Construct the structure of testing and functional reformed plasmids, insert TAL effectors and test the Crown system.</p><br />
<p><strong>Xuemin Pan</strong> Joined in other team members to do the plasmid construction and was responsible for the purification of target protein.</p><br />
<p><strong>Yue Shen</strong> Took part in the design of our project.</p><br />
<p><strong>Karin Yaragi,Xingyu Wang,Jiannan Ye,PeiYu Liu,Yaan Ge,Yan Jiang, Jianye Shi</strong> Responsible for Human Practice and webpage making.</p><br />
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{{Team:SJTU-BioX-Shanghai/footer}}</div>Kariny888