Team:Evry/Interlab Study/08-22-2014

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Picture

Contructions PSB1C3 with I20260, J23101-E1010 and K823012-E1010


Transformation plate observation:
- BBa_J23101: 11 isolated colonies
- BBa_K823012: 100-150 isolated colonies

A PCR was performed on 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.

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1% agarose gel of PCR products of selected colonies. Lane 1 to 8: BBa_K823012 PCR products and Lane 9: Purple 2-Log ladder NEB

We expected to obtain a band between 300 and 400 bp.

Aug 22

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