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From 2014.igem.org

Contents 1 Friday 18th July 1.1 Lab work 1.1.1 1 - Transformation of supercompetent cells with CaCl2 1.1.2 2 - Plasmid DNA Purification 1.1.3 2 - pJBEI 6409 plasmid construction with apramycin

Friday 18th July

Lab work

1 - Transformation of supercompetent cells with CaCl2

by Romain & Arnaud

Strains transformed: MG1655, MG1655Z1. from Bacterial Cultures made the 17th July

Protocol

2 - Plasmid DNA Purification

by Fabio

• J23119 (x2)

from Bacterial Culture made the 17th July

2 - pJBEI 6409 plasmid construction with apramycin

by Sean

Optical density of .134 measured at 10:40am, .619 at 12:30pm.

Electroporation Three samples with varying amounts of plasmids: control, 2μL, 4μL

Protocol

(will check this on Monday... don't edit this part! - SC)

People there:

• Instructors and advisors: Solenne.
• Students: Arnaud, Fabio, Mathias, Romain and Sean.

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