Team:Groningen/Template/MODULE/projects/parts/biobricktable

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Revision as of 21:54, 17 October 2014 by S.Mous (Talk | contribs)

iGEM Groningen 2014 assembled parts
 
 PartTypeDescriptionDesignerLength
 BBa_K1365000CodingnisANisin precursor. Should be used in combination with the other genes on the nisin operon (e.g. in Lactococcus lactis NZ9800).Sandra Mous174
 BBa_K1365003CodingnisCProtein needed in the cyclization of the nisin precursor. Part of the nisin operon.Sandra Mous1245
 BBa_K1365006CodingnisRKTwo proteins involved in the recognition of nisin and positive feedback regulation to induce the PNisA promoter. This BioBrick is part of the nisin operon.Sandra Mous2023
 BBa_K1365020CodingsfGFPSfGFP(Bs) is a for Bacillus optimized version of the high performance sfGFP that works really well in Lactococcus lactis.5Sandra Mous1625
 BBa_K1365100DeviceP2-nisA-dspB-ssUsp45Dual action system against S. aureus, made for expression in L. lactis. Codes for the nisin precursor NisA and the biofilm degrading enzyme DspB. The latter is fused with an USP45sp for transport out of the cell. This device has an P2 promoter and is thus transcribed after binding with p-AgrA.Rick Elbert1625
 BBa_K1365111CodingssUsp45-dspBDispersinB fused to an Usp45sp. The DspB enzyme is secreted from the cell via the usp system of L. lactis6.Rick Elbert1170
 BBa_K1365120DevicePLasR-aiiA-dpsBDual action system against P. aeruginosa for expression in L. lactis and includes the genes for the autoinducer inactivation enzyme A (AiiA) and Dispersin B (DspB).Rick Elbert2350
 BBa_K1365130DevicePLasR-aiiA-dspB-hisSame system as BBa_K1365120, but with 6x histidine tags fused to the two proteins.Rick ElbertEMPTY
 BBa_K1365169CodingssUsp45-aiiAAutoinducer inactivation enzyme A (AiiA) gene fused to signalsequence for the USP45 secretion pathway.Rick Elbert834
 BBa_K1365301PlasmidpIL253Shuttlevector for L. lactis and B. subtilis6. Includes the BioBrick compatible VF2 and RV primer binding sites, prefix and suffix. Contains no illegal restriction sites.Lisa Hielkema6230
EMPTYBBa_K1365555CompositionRBS-sfGFP-dtSfGFP(Bs) combined with RBS and double terminator. This part was used to assemble sfGFP(Bs) with different promoters to characterize the promoters.Aakanksha Saraf874
 BBa_K1365556CompositionRBS-nisA-dtNisA combined with an RBS and double terminator. This part was assembled with different promotors for characterization experiments.Aakanksha Saraf328
 BBa_K1365557ReporterJ23101-sfgfp(bs)SfGFP(Bs) with the constitutive promoter J23101. Designed for use in L. lactis and E. coli.Aakanksha Saraf915
 BBa_K1365997DevicelasRAHL sensor. The LasR membrane protein is produced by this device.Chandhuru Jagadeesan1003
 BBa_K1365998SignalinglasIAHL generator. This part can be used to mimic the presence of AHL producing bacteria P. aeruginosa.Chandhuru Jagadeesan892
 BBa_K1365999CompositionRBS-GFP-dtGFP with RBS and double terminator. This BioBrick is compatible with L. lactis and E. coli.Chandhuru Jagadeesan873