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Team:Groningen/Template/MODULE/project/bandage/visionproblem
From 2014.igem.org
Methods
test 1
test 2
The problem - customer needs
What would the ideal product be
*A product usable for hospitals and customers. To be able to activate in 30 minutes and easy to neutralize
Used strains B. subtilus:
1. ATCC6633 LB
2. ATCC6633 SMM (Preparation of SMM (Spizizen, 1958) with trace elements (Harwood and Archibald, 1990)
3. 168 LB
01-10-2014
- Of all the S. aureus strains two different blood agar plates were made. The first set was named CAL1, NWZ1, CECT1, which meant that per plate 200 μl of sheep blood was added to 10 ml Colorado Blood agar. These were poured in different plates and afterwards 120 μl of each S. aureus strain was streaked out on top of it.
- The second set was named CAL2, NWZ2, CECT2. In these plates 200 μl of sheep blood was added to 10 ml Colorado Blood agar per plate. These were poured on top of 120 μl of each S. aureus strain on each plate separately. This was mixed thoroughly so that the agar was mixed with the strain.
- All the plates were dried under the fumehold.
- On these six plates 6 μl of the three different sets of B. subtilis were spotted and put at 37 ºC for 24h.
02-10-2014
- All the diameters of the halo's were measured and the plates were discarded. (See results below).
