Judging Criteria

Gold Medal

    We measured more about the characterization of an existing part BBa_K515102(PA2652, malate chemoreceptor).We did capillary assay to detect the response of E.coli to malate and succinate and different concentrations of each attractant and compare the result with our part BBa_K1405004.

    We gave The Second High School Attached to Beijing Normal University a lecture, which covered synthetic biology, how to form a high school iGEM team and our program. After classes, they showed eagerness to participate in next year’s high school program and asked us to give more classes after the trip back from Boston. And visiting our lab motivated them to practice and learn more about synthetic biology. We also want to help them to build a senior high iGEM team in future.

    Collaborated and supported the iGEM14_Peking.We helped them to standardize a biobrick BBa_K1378001 expressing MlrA, a 28kDa protease found in Sphingomonas sp which can cleavage microcystins(MCs).

    Collaboration with iGEM14_Peking : As both of our teams focused on E.coil motility, we helped them with a motile model in which E.coil will diffuse freely to the algae. For more collaborations see our collaborations page.

Silver Medal

We have designed, built and experimentally validated two new functional biobricks and submitted characterization data to the parts registry page

BBa_K1405002: encodes a periplasmic binding protein, ModA. And we showed the expression of ModA and its purification by His-chelating chromatography and did the assay by Native-PAGE.

BBa_K1405004: encodes a TCA intermediates responsive chemoreceptor McfR that originally found in Pseudomonas putida. We did the capillary assay to prove its chemotaxis towards malate and succinate.

     We have designed, built and submitted three new biobricks:

BBa_K1405002: encodes a periplasmic binding protein, ModA.

BBa_K1405003: encodes INP derivatives containing only N-domain (INPN).

BBa_K1405004: encodes a TCA intermediates responsive chemoreceptor McfR that originally found in Pseudomonas putida.

     We have designed, and documented three new biobricks:

BBa_K1405005: Linked INPN with ModA via a linker to let ModA stay located at the outer membrane surface.

BBa_K1405006: encodes INPN linked with YFP.

BBa_K1405008: a kill switch in which mazF is inhibited when reduced by IPTG and expressed without IPTG.

     Lab safety：In our lab, all members have received safety trainings before working on our project; experiment regulations are rigidly observed.

     Environment safety, we have evaluated the project feasibility and its relating security issues. We designed a kill switch to guarantee our “Prometheus” is under control while capturing Molybdenum and delivering it to roots of legumes. The kill switch is supposed to be “off” for a certain time in the soil, so the bacteria will gain enough time to perform its function.

     We designed the kill switch, submitted our safety documentation and published general safety information on our wiki safety page.

The Story of E.coli Prometheus

BNU-China