Team:Groningen/Template/MODULE/Notebook/toolbox/appendix/table3

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The primers are denoted with the position of the illegal restriction site that they should remove. Illegal EcoRI sites are abbreviated with an E, XbaI sites with an X, SpeI site with an S and PstI sites with a P.
Primers for making BioBricks out of genes with illegal restriction sites
 
Gene Primer Sequence
NisT Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGATGAAGTGAAAGAATTTACATCA
  S980 reverse TTGTTCCATAAACAAACTTGTATTATAAATGATGTAAATA
  S980 forward TATTTACATCATTTATAATACAAGTTTGTTTATGGAACAA
  S1210 reverse CCATAGTTGGTTGATATAATCCTGAAATTATCTTTACTTGTGTAC
  S1210 forward GTACACAAGTAAAGATAATTTCAGGATTATATCAACCAACTATGG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTCATCATTATCCT
  Suffix forward ATGAATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse CACTTCATCCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisP Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGGTGAAAAAAATACTAGGTTT
  P225 reverse AGTCCTTGTTGTCGATTTTACTGCTGGTGACTGCGCCCCT
  P225 forward AGGGGCGCAGTCACCAGCAGTAAAATCGACAACAAGGACT
  E347 reverse AACGCTATCTCTCTTACTAAATTCAGAACTAACTTGAGTT
  E347 forward AACTCAAGTTAGTTCTGAATTTAGTAAGAGAGATAGCGTT
  E1657 reverse CAATACTCTATTCCCATTAACTTCTGGACTATTCATTAGA
  E1657 forward TCTAATGAATAGTCCAGAAGTTAATGGGAATAGAGTATTG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCAATTTTTAGTCTTTC
  Suffix forward AAATTGATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse CCTAGTATTTTTTTCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG
NisFEG Prefix forward GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA
  P186 reverse TCCAGTATCAGCAGAAATTAAACCAAACAAAATTTTCATC
  P186 forward GATGAAAATTTTGTTTGGTTTAATTTCTGCTGATACTGGA
  E336/X349 reverse CCTGTTTCTGCCAAACCAATCACTTCTAGTGTTTCATGTATTCTCTTA
  E336/X349 forward TAAGAGAATACATGAAACACTAGAAGTGATTGGTTTGGCAGAAACAGG
  Suffix reverse GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT
  Suffix forward TAGATAATAATACTAGTAGCGGCCGCTGCAGGAAGAAACAAAAGGGCAA
  Prefix reverse TTTTACCTGCATCTAGAAGCGGCCGCGAATTCGAAGAAACATCCTTAGCG