Team:York/Notebook
From 2014.igem.org
Notebook
Week 1
This week we transformed DNA from BBA_J04450 (RFP construct) in plasmid backbone pSB1C3 to test how efficient our competent cells were.
Week 2
In week two we transformed E. coli with DNA from BBA_K592009 amilcp.
Week 3
Our team were at YSB for the majority of this week therefore no lab work took place.
Week 4
We transformed E. coli with pSB1K3 and pSB1A3 so we were able to again test how efficient our competent cells were as we had had to make up more competent cells.