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Team:Korea U Seoul/hp/safty Q
From 2014.igem.org
Would any of your project ideas raise safety issues in terms of:
- Researcher Safety
- EtBr EtBr: Ethidium bromide is used as a fluorescent tag for gel electrophoresis in our lab. EtBr is known to negatively affect important biological processes, such as DNA replication and transcription, because it intercalates and deforms double-stranded DNA. Though we wash our agarose gels with EtBr solution, we strictly make sure that the solution does not make contact with human body by wearing gloves when handling the solution. The gels are safely discarded according to the safety guideline provided by the university.
- Public Safety Genetically modified bacteria are strictly prevented from being released out of the lab.
- Environment Safety We strictly follow the safety guideline provided by the university to take appropriate means of safely discarding all experiment waste.
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? how did you manage to handle the safety issue? How could other teams learn from your experience?
BioBrick we made is Carbonic Anhydrase from nacreous layer in oyster pearls. It can hold CO2 by attach that by CaCO3 form. So CO2 fixed by nacrein have special crystal structure because of nacrein between the CaCO3. Nacrein only action as a Carbonic Anhydrase and it is one of the most universal enzyme. One difference is using Ca to fix CO2. But CaCO3 also popular chemical, So there's no risk for nature.
Also it is hard to express in other species because of the vector which we use need special material to active.
Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?
- Regarding local biosafety group There is no biosafety group, committee, or review board at our institution. The iGEM project has not been reviewed by any local bio-safety committee. However, we follow the bio-safety guidelines for any procedure in the laboratory. Please refer to our safety guideline (includes what biosafety rules we have to consider in our country): http://kusafe.korea.ac.kr/main.asp (university guideline, Korean) http://bric.postech.ac.kr/myboard/read.php?Board=safety&id=1481(national guideline, Korean)
- Consideration about biosafety rules or guidelines Based on our university guideline and national guideline, we made 3 major biosafety principles and their detailed guideline.
- Biosafety level of laboratory This principle defines boundary of experiment which labs can conducted and limits the type of experiments based on their capability. Each lab should be evaluated for their biosafety capability and assigned any level of biosafety to conduct an experiment. Based on their capability and assigned biosafety level, types of experiments that they can do should be limited. For example, a lab assigned to lowest biosafety level cannot handle pathogenic bacteria.
- Assessment and evaluation of project and pathogenicity of bacteria This principle assess and evaluate biosafety of project and pathogenicity of bacteria. When assessing a project, one should evaluate potential threat for researcher, public, and ecosystem. Like medicinal project undergoing IRB examination before the experiment, biological experiment should go though series of assessment and evaluation. Biosafety committees and scientists should communicate each other and give them thorough feedback.
- Bio-security This principle covers securing biological organism from lost, theft, and misuse. It also deals with regulation after lost, theft, and misuse is happened. To achieve this, bio-security system should be established.
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
- Suggestions for safety issue Safety is the most important part of iGEM competition. Every member of team should know and follow the safety rules. Here are recommendations we give to the future iGEM teams
- Every member should know about safety rules in the lab. This includes bio-security level, pathogen, gene product, protocol, and etc.
- Each member should know bio-security level of lab. Can lab deal with pathogen? Does the lab have proper equipments for experiment? This kind of consideration is recommended before designing and conducting experiments. Conducting a survey is recommended.
- Each member should be careful if they are dealing with pathogen. How can we prevent disease outbreak? What are safety requirements? Please study about pathogen and related diseases beforehand.
- Each member should consider their gene product. Gene product may be chemically dangerous and may damage ecosystem.
- Each member should be aware of protocols of instruments to avoid any accidents. This is also related to lab results.
- Communication matters. Miscommunication leads to accidents and poor results. Results of the experiment should be reviewed and shared by every member of the group. Weekly group discussion is recommended. Each member should know everything about the experiment before one actually does it.
- How could parts, devices, and systems be made even safer though bio-safety engineering?
- Changing promoter or other regulatory mechanism to shorten the duration of mRNA Changing promoter sequence effectively controls survival time of mRNA and overall production of protein. If mRNA degrades easily, we can control it.
- Adding ‘time bomb’ to kill themselves as time goes. Internal clock bomb will ensure death of our product. So it will be much safer.
