Team:ETH Zurich/data
From 2014.igem.org
Data Page
Gene Circuit
For more information about the parts used in this circuit click on the parts in the picture that link to the [http://parts.igem.org/Main_Page registry].
Click here to see in action how the circuit responds to different inputs.
Our Favorite New Parts
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Used and Characterized Pre-Existing Parts
- [http://parts.igem.org/Part:BBa_R0062:Experience Experience] - Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062: the promoter's EC50 could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system by using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR. The amount of regulator also demonstrated an influence on the promoter's basal expression or leakiness. Additionally, it could be determined that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] thus showing crosstalk with these two quorum sensing systems.
- [http://parts.igem.org/Part:BBa_R0079:Experience Experience] - Promoter (LasR & 3OC12-HSL regulated), BBa_R0079: it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)].
Characterized new parts
- there