Team:Penn State/Notebook

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Penn State iGEM 2014 Notebook Page

Biodetoxification
Codon Optimization
Wednesday, May 21, 2014
Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA ...................
Thursday, May 22, 2014
Ashlee and Emily performed a transformation
Friday, May 23, 2014
We picked several colonies for overnight growth to do more cloning tomorrow
Saturday, May 24, 2014
Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion
Monday, May 25, 2014
Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination.