Team:Groningen/Template/MODULE/Project/secretion/part4

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The construct
In order to synchronize the secretion of infection preventing molecules with the detection of the certain pathogens, a few conditions must been set.

  • The construct should be regulated through detection.
  • It must secrete DspB when L. lactis senses both P. aeruginosa and S. aureus
  • It must secrete Aiia when it detect P. aeruginosa
  • It must secrete Nisin when it detects S. aureus

After designing the systems, two constructs came out: The anti-Pseudomonas aeruginosa system:
Figure 6
 
Figure 6: The DspB and AiiA construct
 
 
The anti-staphylococcus aureus secretion system:
Figure 7
 
Figure 7: The DspB and nisin construct