Team:Manchester/main.html

From 2014.igem.org

(Difference between revisions)
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         <style type="text/css">
         <style type="text/css">
-
/*
 
-
** MediaWiki 'monobook' style sheet for CSS2-capable browsers.
 
-
** Copyright Gabriel Wicke - http://wikidev.net/
 
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** License: GPL (http://www.gnu.org/copyleft/gpl.html)
 
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**
 
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** Loosely based on http://www.positioniseverything.net/ordered-floats.html by Big John
 
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** and the Plone 2.0 styles, see http://plone.org/ (Alexander Limi,Joe Geldart & Tom Croucher,
 
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** Michael Zeltner and Geir Bækholt)
 
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** All you guys rock :)
 
-
*/
 
-
 
-
 
-
/* Font size:
 
-
** We take advantage of keyword scaling- browsers won't go below 9px
 
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** More at http://www.w3.org/2003/07/30-font-size
 
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** http://style.cleverchimp.com/font_size_intervals/altintervals.html
 
-
*/
 
-
 
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body {
 
-
 
-
 
-
/* scale back up to a sane default */
 
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#globalWrapper {
 
-
 
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}
 
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.visualClear {
 
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    clear: both;
 
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}
 
-
 
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/* The top section contains the banner, the search box, and the left and right menus */
 
-
 
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#top-section {
 
-
 
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}
 
-
 
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#p-logo {
 
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}
 
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#p-logo h5 {
 
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}
 
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#p-logo a,
 
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#p-logo a:hover {
 
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}
 
-
 
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#search-controls {
 
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}
 
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input.searchButton {
 
-
 
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}
 
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input.searchButton:hover {
 
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}
 
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#searchInput {
 
-
 
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}
 
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#menubar {
 
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}
 
-
 
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.left-menu, .left-menu a {
 
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}
 
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.left-menu:hover {
 
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}
 
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.right-menu, .right-menu a {
 
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}
 
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#menubar ul {
 
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}
 
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#menubar li {
 
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}
 
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.left-menu li a {
 
-
   
 
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}
 
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.left-menu .selected {
 
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}
 
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#.left-menu .selected:hover {
 
-
 
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#}
 
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.left-menu:hover a {
 
-
   
 
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}
 
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.right-menu li {
 
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#   
 
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}
 
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.right-menu li a {
 
-
   
 
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}
 
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.right-menu li a:hover {
 
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    }
 
-
 
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/* footer */
 
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#footer-box {
 
-
 
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}
 
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#footer {
 
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}
 
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#footer li {
 
-
 
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}
 
-
 
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/* Sponsors box */
 
-
 
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#sponsor-box {
 
-
   
 
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}
 
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#f-poweredbyico {
 
-
   
 
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}
 
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#f-copyrightico {
 
-
   
 
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}
 
-
 
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/* Pulldown menu boxes */
 
-
 
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.hidden  { visibility: hidden;  }
 
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.visible {      visibility: visible; }
 
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-
 
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.menubox {
 
-
 
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}
 
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.bridge1 {
 
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}
 
-
 
-
 
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.shadow1 {
 
-
 
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}
 
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.shadow2 {
 
-
 
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}
 
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.shadow3 {
 
-
 
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}
 
-
 
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/*  IGEM RIGHT SIDEBAR STYLING */
 
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#right-sidebar {
 
-
 
-
}
 
-
.right-box {
 
-
 
-
}
 
-
 
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.right-box:first-child {
 
-
 
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}
 
-
 
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.right-heading {
 
-
 
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}
 
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.right-box ul {
 
-
 
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}
 
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.right-box li {
 
-
 
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}
 
-
 
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/*****************************************************************************************************
 
-
* The content div contains all of the contents of the page - provided by mediawiki
 
-
****************************************************************************************************/
 
-
#content {
 
-
   
 
-
}
 
-
 
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#column-one {
 
-
   
 
-
}
 
-
/* the left column width is specified in class .portlet */
 
-
 
-
/* general styles */
 
-
 
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table {
 
-
 
 
-
}
 
-
a {
 
-
 
-
}
 
-
a:visited {
 
-
   
 
-
}
 
-
a:active {
 
-
   
 
-
}
 
-
a:hover {
 
-
 
-
}
 
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a.stub {
 
-
 
-
}
 
-
/*  a.new, #p-personal a.new {    - rdr */
 
-
a.new {
 
-
 
-
}
 
-
a.new:visited, #p-personal a.new:visited {
 
-
 
-
}
 
-
 
-
img {
 
-
   
 
-
 
-
}
 
-
p {
 
-
 
 
-
}
 
-
p img {
 
-
   
 
-
}
 
-
 
-
hr {
 
-
 
 
-
}
 
-
 
-
h1, h2, h3, h4, h5, h6 {
 
-
 
 
-
}
 
-
h1 { }
 
-
h2 { }
 
-
h3, h4, h5, h6 {
 
-
   
 
-
}
 
-
h3 {  }
 
-
h4 { }
 
-
h5 {  }
 
-
h6 { }
 
-
 
-
ul {
 
-
   
 
-
}
 
-
ol {
 
-
 
 
-
}
 
-
li {
 
-
   
 
-
}
 
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dt {
 
-
 
 
-
}
 
-
dl {
 
-
 
 
-
}
 
-
dd {
 
-
 
-
}
 
-
 
-
fieldset {
 
-
 
 
-
}
 
-
legend {
 
-
 
 
-
}
 
-
form {
 
-
 
 
-
}
 
-
 
-
textarea {
 
-
 
 
-
}
 
-
 
-
input.historysubmit {
 
-
 
 
-
}
 
-
select {
 
-
   
 
-
}
 
-
abbr, acronym, .explain {
 
-
 
 
-
}
 
-
q {
 
-
   
 
-
}
 
-
/* disabled for now
 
-
blockquote {
 
-
    font-family: Times, "Times New Roman", serif;
 
-
    font-style: italic;
 
-
}*/
 
-
code {
 
-
   
 
-
}
 
-
pre {
 
-
   
 
-
}
 
-
 
-
/*
 
-
** the main content area
 
-
*/
 
-
 
-
#siteSub {
 
-
  }
 
-
#contentSub {
 
-
   
 
-
}
 
-
span.subpages {
 
-
    display: block;
 
-
}
 
-
 
-
/* Some space under the headers in the content area */
 
-
#bodyContent h1, #bodyContent h2 {
 
-
    display: none;
 
-
 
 
-
}
 
-
#bodyContent h3, #bodyContent h4, #bodyContent h5 {
 
-
  display: none;
 
-
}
 
-
.firstHeading {
 
-
    display: none;
 
-
}
 
-
 
-
/* user notification thing */
 
-
.usermessage {
 
-
  display: none;
 
-
}
 
-
#siteNotice {
 
-
    display: none;
 
-
}
 
-
#siteNotice p {
 
-
    display: none; 
 
-
}
 
-
.error {
 
-
    display: none;
 
-
}
 
-
#catlinks {
 
-
  display: none;
 
-
}
 
-
/* currently unused, intended to be used by a metadata box
 
-
in the bottom-right corner of the content area */
 
-
.documentDescription {
 
-
    /* The summary text describing the document */
 
-
 
 
-
}
 
-
.documentByLine {
 
-
   
 
-
}
 
-
 
-
/* emulate center */
 
-
.center {
 
-
    display: none;
 
-
}
 
-
*.center * {
 
-
    display: none;
 
-
}
 
-
/* small for tables and similar */
 
-
.small, .small * {
 
-
  display: none;
 
-
}
 
-
table.small {
 
-
    display: none;
 
-
}
 
-
 
-
/*
 
-
** content styles
 
-
*/
 
-
 
-
#toc,
 
-
.toc {
 
-
display: none;
 
-
}
 
-
#toc h2,
 
-
.toc h2 {
 
-
display: none;
 
-
}
 
-
#toc #toctitle,
 
-
.toc #toctitle,
 
-
#toc .toctitle,
 
-
.toc .toctitle {
 
-
display: none;
 
-
}
 
-
#toc ul,
 
-
.toc ul {
 
-
display: none;
 
-
}
 
-
#toc ul ul,
 
-
.toc ul ul {
 
-
display: none;
 
-
}
 
-
#toc .toctoggle,
 
-
.toc .toctoggle {
 
-
display: none;
 
-
}
 
-
 
-
 
-
/* images */
 
-
div.floatright, table.floatright {
 
-
  display: none;
 
-
/*
 
-
    border: .5em solid white;
 
-
    border-width: .5em 0 .8em 1.4em;
 
-
*/
 
-
}
 
-
div.floatright p { display: none; }
 
-
div.floatleft, table.floatleft {
 
-
  display: none;
 
-
/*
 
-
    margin: .3em .5em .5em 0;
 
-
    border: .5em solid white;
 
-
    border-width: .5em 1.4em .8em 0;
 
-
*/
 
-
}
 
-
div.floatleft p { display: none;}
 
-
/* thumbnails */
 
-
div.thumb {
 
-
  display: none;
 
-
}
 
-
div.thumb div {
 
-
    display: none;
 
-
}
 
-
div.thumb div a img {
 
-
  display: none;
 
-
}
 
-
div.thumb div div.thumbcaption {
 
-
  display: none;
 
-
}
 
-
div.magnify {
 
-
  display: none;
 
-
}
 
-
div.magnify a, div.magnify img {
 
-
    display: none;
 
-
}
 
-
div.tright {
 
-
    display: none;
 
-
}
 
-
div.tleft {
 
-
    display: none;
 
-
}
 
-
 
-
.hiddenStructure {
 
-
  display: none;
 
-
}
 
-
img.tex {
 
-
  display: none;
 
-
}
 
-
span.texhtml {
 
-
    display: none;
 
-
}
 
-
 
-
/*
 
-
** classes for special content elements like town boxes
 
-
** intended to be referenced directly from the wiki src
 
-
*/
 
-
 
-
/*
 
-
** User styles
 
-
*/
 
-
/* table standards */
 
-
table.rimage {
 
-
  display: none;
 
-
}
 
-
.toccolours {
 
-
    display: none;
 
-
}
 
-
div.townBox {
 
-
    display: none;
 
-
}
 
-
div.townBox dl {
 
-
  display: none;
 
-
}
 
-
div.townBox dl dt {
 
-
  display: none;
 
-
}
 
-
div.townBox dl dd {
 
-
    display: none;
 
-
}
 
-
 
-
/*
 
-
** edit views etc
 
-
*/
 
-
.special li {
 
-
  display: none;
 
-
}
 
-
 
-
/* Page history styling */
 
-
/* the auto-generated edit comments */
 
-
.autocomment {
 
-
 
 
-
}
 
-
#pagehistory span.user {
 
-
 
 
-
}
 
-
#pagehistory span.minor {
 
-
 
 
-
}
 
-
#pagehistory li {
 
-
   
 
-
}
 
-
#pagehistory li.selected {
 
-
 
 
-
}
 
-
 
-
/*
 
-
** Diff rendering
 
-
*/
 
-
table.diff, td.diff-otitle, td.diff-ntitle {
 
-
    background-color: white;
 
-
}
 
-
td.diff-addedline {
 
-
    background: #cfc;
 
-
    font-size: smaller;
 
-
}
 
-
td.diff-deletedline {
 
-
    background: #ffa;
 
-
    font-size: smaller;
 
-
}
 
-
td.diff-context {
 
-
    background: #eee;
 
-
    font-size: smaller;
 
-
}
 
-
span.diffchange {
 
-
    color: red;
 
-
    font-weight: bold;
 
-
}
 
-
 
-
/*
 
-
** keep the whitespace in front of the ^=, hides rule from konqueror
 
-
** this is css3, the validator doesn't like it when validating as css2
 
-
*/
 
-
#bodyContent a[href ^="http://"],
 
-
#bodyContent a[href ^="gopher://"] {
 
-
/*    background: url(external.png) center right no-repeat;    rdr */
 
-
/*    padding-right: 13px;                                    rdr */
 
-
}
 
-
#bodyContent a[href ^="https://"],
 
-
.link-https {
 
-
    background: url(lock_icon.gif) center right no-repeat;
 
-
    padding-right: 16px;
 
-
}
 
-
#bodyContent a[href ^="mailto:"],
 
-
.link-mailto {
 
-
    background: url(mail_icon.gif) center right no-repeat;
 
-
    padding-right: 18px;
 
-
}
 
-
#bodyContent a[href ^="news://"] {
 
-
    background: url(news_icon.png) center right no-repeat;
 
-
    padding-right: 18px;
 
-
}
 
-
#bodyContent a[href ^="ftp://"],
 
-
.link-ftp {
 
-
    background: url(file_icon.gif) center right no-repeat;
 
-
    padding-right: 18px;
 
-
}
 
-
#bodyContent a[href ^="irc://"],
 
-
.link-irc {
 
-
    background: url(discussionitem_icon.gif) center right no-repeat;
 
-
    padding-right: 18px;
 
-
}
 
-
/* disable interwiki styling */
 
-
#bodyContent a.extiw,
 
-
#bodyContent a.extiw:active {
 
-
    color: #36b;
 
-
    background: none;
 
-
    padding: 0;
 
-
}
 
-
/* this can be used in the content area to switch off
 
-
special external link styling */
 
-
#bodyContent .plainlinks a {
 
-
    background: none !important;
 
-
    padding: 0;
 
-
}
 
-
 
-
/* the icon in front of the user name, single quotes
 
-
in bg url to hide it from iemac */
 
-
li#pt-userpage,
 
-
li#pt-anonuserpage,
 
-
li#pt-login {
 
-
    padding-left: 20px;
 
-
    text-transform: none;
 
-
}
 
-
 
-
 
-
/* hide from incapable browsers */
 
-
head:first-child+body #footer li { white-space: nowrap; }
 
-
 
-
 
-
/* js pref toc */
 
-
#preftoc {
 
-
    float: left;
 
-
    margin: 1em;
 
-
    width: 13em;
 
-
}
 
-
#preftoc li {
 
-
    border: 1px solid white;
 
-
}
 
-
#preftoc li.selected {
 
-
    background-color: #f9f9f9;
 
-
    border: 1px dashed #aaa;
 
-
}
 
-
#preftoc a,
 
-
#preftoc a:active {
 
-
    display: block;
 
-
    color: #0014a6;
 
-
}
 
-
#prefcontrol {
 
-
    clear: both;
 
-
    float: left;
 
-
    margin-top: 1em;
 
-
}
 
-
div.prefsectiontip {
 
-
    font-size: 95%;
 
-
    margin-top: 1em;
 
-
}
 
-
fieldset.operaprefsection {
 
-
    margin-left: 15em;
 
-
}
 
-
 
-
/*
 
-
** IE/Mac fixes, hope to find a validating way to move this
 
-
** to a separate stylesheet. This would work but doesn't validate:
 
-
** @import("IEMacFixes.css");
 
-
*/
 
-
/* tabs: border on the a, not the div */
 
-
* > html #p-cactions li { border: none; }
 
-
* > html #p-cactions li a {
 
-
    border: 1px solid #aaa;
 
-
    border-bottom: none;
 
-
}
 
-
 
-
/* * > html #p-cactions li.selected a { border-color: #00bd23; }  */
 
-
/* footer icons need a fixed width */
 
-
* > html #f-poweredbyico,
 
-
/* * > html #f-copyrightico { width: 88px; } */
 
-
* > html #bodyContent,
 
-
* > html #bodyContent pre {
 
-
    overflow-x: auto;
 
-
    width: 100%;
 
-
    padding-bottom: 25px;
 
-
}
 
-
 
-
/* more IE fixes */
 
-
/* float/negative margin brokenness */
 
-
* html #footer {margin-top: 0;}
 
-
* html #column-content {
 
-
    display: inline;
 
-
    margin-bottom: 0;
 
-
}
 
-
* html div.editsection { font-size: smaller; }
 
-
#pagehistory li.selected { position: relative; }
 
-
 
-
/* Mac IE 5.0 fix; floated content turns invisible */
 
-
* > html #column-content {
 
-
    float: none;
 
-
}
 
-
* > html #column-one {
 
-
    position: absolute;
 
-
    left: 0;
 
-
    top: 0;
 
-
}
 
-
* > html #footer {
 
-
    margin-left: 13.2em;
 
-
}
 
-
.redirectText {
 
-
    font-size: 150%;
 
-
    margin: 5px;
 
-
}
 
-
 
-
.printfooter {
 
-
    display: none;
 
-
}
 
-
 
-
.not-patrolled {
 
-
    background-color: #ffa;
 
-
}
 
-
div.patrollink {
 
-
    font-size: 75%;
 
-
    text-align: right;
 
-
}
 
-
span.newpage, span.minor, span.searchmatch {
 
-
    font-weight: bold;
 
-
}
 
-
span.unpatrolled {
 
-
    font-weight: bold;
 
-
    color: red;
 
-
}
 
-
 
-
span.searchmatch {
 
-
    color: red;
 
-
}
 
-
.sharedUploadNotice {
 
-
    font-style: italic;
 
-
}
 
-
 
-
span.updatedmarker {
 
-
    color: black;
 
-
    background-color: #0f0;
 
-
}
 
-
span.newpageletter {
 
-
    font-weight: bold;
 
-
    color: black;
 
-
    background-color: yellow;
 
-
}
 
-
span.minoreditletter {
 
-
    color: black;
 
-
    background-color: #c5ffe6;
 
-
}
 
-
 
-
table.gallery {
 
-
    border: 1px solid #ccc;
 
-
    margin: 2px;
 
-
    padding: 2px;
 
-
    background-color: white;
 
-
}
 
-
 
-
table.gallery tr {
 
-
    vertical-align: top;
 
-
}
 
-
 
-
table.gallery td {
 
-
    vertical-align: top;
 
-
    background-color: #f9f9f9;
 
-
    border: solid 2px white;
 
-
}
 
-
 
-
div.gallerybox {
 
-
    margin: 2px;
 
-
    width:  150px;
 
-
}
 
-
 
-
div.gallerybox div.thumb {
 
-
    text-align: center;
 
-
    border: 1px solid #ccc;
 
-
    margin: 2px;
 
-
}
 
-
 
-
div.gallerytext {
 
-
    font-size: 94%;
 
-
    padding: 2px 4px;
 
-
}
 
-
 
-
span.comment {
 
-
font-style: italic;
 
-
}
 
-
 
-
span.changedby {
 
-
font-size: 95%;
 
-
}
 
-
 
-
.previewnote {
 
-
text-align: center;
 
-
color: #cc0000;
 
-
}
 
-
.editExternally {
 
-
border: 1px solid gray;
 
-
background-color: #ffffff;
 
-
padding: 3px;
 
-
margin-top: 0.5em;
 
-
float: left;
 
-
font-size: small;
 
-
text-align: center;
 
-
}
 
-
.editExternallyHelp {
 
-
font-style: italic;
 
-
color: gray;
 
-
}
 
-
 
-
li span.deleted {
 
-
text-decoration: line-through;
 
-
color: #888;
 
-
font-style: italic;
 
-
}
 
-
 
-
.toggle {
 
-
margin-left: 2em;
 
-
text-indent: -2em;
 
-
}
 
-
 
-
/* Classes for EXIF data display */
 
-
table.metadata {
 
-
font-size: 0.8em;
 
-
margin-left: 0.5em;
 
-
margin-bottom: 0.5em;
 
-
}
 
-
 
-
table.metadata caption {
 
-
font-weight: bold;
 
-
}
 
-
 
-
table.metadata th {
 
-
font-weight: normal;
 
-
}
 
-
 
-
table.metadata td {
 
-
padding: 0.1em;
 
-
}
 
-
 
-
table.metadata {
 
-
border: none;
 
-
border-collapse: collapse;
 
-
}
 
-
 
-
table.metadata td, table.metadata th {
 
-
text-align: center;
 
-
border: 1px solid #aaaaaa;
 
-
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Revision as of 21:18, 13 August 2014

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University of Manchester

Meet The Team

Project Overview

Battling against obesity

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Project Overview

Overall Aims:

To engineer the commensal E. coli, Nissle 1917 to absorb large volumes of glucose (and galactose).
This will enable the bacteria to function like a sugar sponge in the intestines sequestering sugar
and preventing it from being absorbed.
We will look at this in respects to a treatment for obesity and reducing calorific intake.


Biobricks used:

osmY (full sequence) Part:BBa_J45992:

  • Full length sequence of the osmY stationary phase promoter.
    This promoter recruits RNAP with the sigmas haloenzyme which is expressed mainly during periods of low growth or starvation.
    It is also slightly activated by the sigma 70 exponential phase RNAP as well.
  • We will use this to control expression of our mutant CRP so it is only expressed once the cells are fully grown and not during the growth of the Nissle.


New Biobricks:

CRP (cAMP reactive protein/peptide also known as CAP (cAMP activating protein/peptide)).

  • WT CRP will be used mainly as a control to compare to the effects of the mutant CRP.
    CRP can only bind DNA when it is activated by binding to cAMP. cAMP levels are controlled by adenylate cyclase which itself is controlled by enzyme IIa part of the glucose uptake system in E. coli.


T127I/S128I Mutant CRP

  • This mutant version of CRP is constitutively active regardless of the levels of cAMP.
    This means that it can induce glucose uptake regardless of the levels of cAMP in the cell and therefore the cells become glucose insensitive and absorb excessive volumes of glucose.


GlmS Riboswitch (from B. Subtilis)

  • The GlmS riboswitch is found in most gram positive bacteria.
    It is found in the 3’-UTR of the GlmS gene which is responsible for increasing the levels of glucosamine-6-phosphate, which is used to make cell walls etc. Normally when GLcN-6P is present it binds to the riboswitch and causes cleavage and degradation of the mRNA.
    Glucose can also bind to the riboswitch but it prevents cleavage and allows transcription. We will use this to ensure that our gene is only expressed in high glucose conditions.


GlmS Promoter (from B. Subtilis)

  • Normal GlmS promoter, this will be tested in parallel with the osmY stationary phase promoter and will be used to express the riboswitch and CRP.


GFP GlmS Riboswitch Fusion

  • Reporter to analyse the effects of the riboswitch in different concentrations of glucose and to determine how much cleavage may occur in the intestines.


Mutant CRP GlmS Fusion

  • Final product (+promoter) will be inserted into Nissle 1917.


Meet The Team

Greta

Greta Jasulaityte

Greta is in her 2nd year studying BSc Biotechnology.
Her role in the project is doing the lab work and modelling.
In her free time she enjoys travelling and salsa dancing.

Catherine

Catherine Jeon

Catherine is in her final year studyinng BSc Sociology and she is working on the Human Practices side of the project.
She really enjoys traveling; whenever time and money allows!

Izzy

Isobel Holley

Izzy is in her first year studying Neuroscience.

James

James Parkinson

James is in his final year studying BSc Biomedical Science and his role in the team is carrying out the experiments.
Besides doing sciencey stuff he enjoys having loooong (not that long) walks on the beach.

Sid

SiddharthYadav

Sid is in his 1st year doing the B.Soc.Sc. Sociology course.
He is working side by side with Catherine on the Human Practices aspect of it.
Currently he is in the 110 metre Hurdles team and he enjoys history, movies, travelling and having fun (all sorts).

Taf

Tafadzwa Machiridza

Taf (That's me) is in his 2nd year studying Computer Science and Maths.
I'm the guy who's designing the wiki pages. I LOVE my food and sleep. Besides that, I like playing video games, playing football, dancing and doing pencil sketches.

Eriko

Eriko Takano

More info about Eriko.

Rainer

Rainer Breitling

More info about Rainer.

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