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Team:SYSU-China/Project
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<p>Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)</p> | <p>Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)</p> | ||
<p>The contemporary methods of directed evolution of certain protein require much work by people. This summer, SYSU-China is trying to construct an in vitro system which can allow us to directly obtain a particular protein in a simple and convenient way. The key of this system is to turn protein interaction signal into the advantage of evolution. We chose the bacteria two hybrid system to transfer the protein interaction signal into the expression of a target gene, gene II from M13 bacteria phage, which is a key factor for phage DNA replication. By knocking out the gene II in M13 bacteria phage genome and expressing gene II under the control of the bacteria two hybrid system, we can successfully convert the protein interaction signal into the advantage of M13 phage generation. The M13 phage generated in our system carry the protein coding sequence needed to evolve. As the system operating, the phage contained desirable protein coding sequence can grow into dominant population.</p> | <p>The contemporary methods of directed evolution of certain protein require much work by people. This summer, SYSU-China is trying to construct an in vitro system which can allow us to directly obtain a particular protein in a simple and convenient way. The key of this system is to turn protein interaction signal into the advantage of evolution. We chose the bacteria two hybrid system to transfer the protein interaction signal into the expression of a target gene, gene II from M13 bacteria phage, which is a key factor for phage DNA replication. By knocking out the gene II in M13 bacteria phage genome and expressing gene II under the control of the bacteria two hybrid system, we can successfully convert the protein interaction signal into the advantage of M13 phage generation. The M13 phage generated in our system carry the protein coding sequence needed to evolve. As the system operating, the phage contained desirable protein coding sequence can grow into dominant population.</p> | ||
| - | <p>What’s more, in order to make this system more efficient and more controllable, we introduce several proteins to raise the mutation rate and | + | <p>What’s more, in order to make this system more efficient and more controllable, we introduce several proteins to raise the mutation rate and use RNA thermometer to control gene II expression in the dimension of temperature.</p> |
<p>This system can have broad applications. It may be able the generate antibody that we cannot obtain in normal way, for example, the HIV antibody. We can integrate HIV antigen into our system and generate the corresponding antibody. </p> | <p>This system can have broad applications. It may be able the generate antibody that we cannot obtain in normal way, for example, the HIV antibody. We can integrate HIV antigen into our system and generate the corresponding antibody. </p> | ||
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Revision as of 06:11, 12 August 2014
WELCOME TO iGEM 2014!Your team has been approved and you are ready to start the iGEM season!
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs) The contemporary methods of directed evolution of certain protein require much work by people. This summer, SYSU-China is trying to construct an in vitro system which can allow us to directly obtain a particular protein in a simple and convenient way. The key of this system is to turn protein interaction signal into the advantage of evolution. We chose the bacteria two hybrid system to transfer the protein interaction signal into the expression of a target gene, gene II from M13 bacteria phage, which is a key factor for phage DNA replication. By knocking out the gene II in M13 bacteria phage genome and expressing gene II under the control of the bacteria two hybrid system, we can successfully convert the protein interaction signal into the advantage of M13 phage generation. The M13 phage generated in our system carry the protein coding sequence needed to evolve. As the system operating, the phage contained desirable protein coding sequence can grow into dominant population. What’s more, in order to make this system more efficient and more controllable, we introduce several proteins to raise the mutation rate and use RNA thermometer to control gene II expression in the dimension of temperature. This system can have broad applications. It may be able the generate antibody that we cannot obtain in normal way, for example, the HIV antibody. We can integrate HIV antigen into our system and generate the corresponding antibody. ReferencesiGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. |
You can use these subtopics to further explain your project
It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work. It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted. |
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