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Team:Paris Saclay/Notebook/July/24
From 2014.igem.org
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
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===? - ?????=== | ===? - ?????=== | ||
====Gel electrophoresis of p cola==== | ====Gel electrophoresis of p cola==== | ||
| - | + | [[File:LU000081.jpg|right]] | |
''by Sean'' | ''by Sean'' | ||
| - | p cola used: sample left overnight [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23 yesterday] resuspended in 30μl of H<2>O milliQ. | + | p cola used: sample left overnight [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23 yesterday] resuspended in 30μl of H<sub>2</sub>O milliQ. |
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol] | ||
| - | Results | + | '''Results''' |
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| + | # | ||
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| + | '''Members present:''' | ||
| + | * Instructors and advisors: Alice and Solenne. | ||
| + | * Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry. | ||
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | [https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar] | ||
Revision as of 14:11, 24 July 2014
Contents |
Thursday 24th July
Lab work
C - Lemon scent
Results of the PCR Targeting
by Romain
PCR targeting made the July 22nd.
Results:
- Nothing has grown on the control dishes.
- Failure, nothing has grown on the clone I dishes.
- Failure, other bacteria have grown on the clone II dishes.
We have to make another PCR.
E - The Suppressing Salicylate System
Transformation of competent E.coli cells
by Fabio
We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).
Samples:
- BBa_J61051 Concentrated
- BBa_J61051 Undiluted
- BBa_K228001 Concentrated
- BBa_K228001 Undiluted
- DH5α Control
? - ?????
Gel electrophoresis of p cola
by Sean
p cola used: sample left overnight yesterday resuspended in 30μl of H2O milliQ.
Results
Members present:
- Instructors and advisors: Alice and Solenne.
- Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.
