Talk:Team:TCU Taiwan/M13Phage
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<td valign="top"><font size="3" face="Verdana" color="#333"><p>When a normal M13 phage infect <em>E.coli</em><em> with </em>F plasmid(strain JM101 in our experiment), it will use F pilus to put its genome into cytosol. Then this single strand genome will use host’s polymerase to make itself a double strand structure and stay in cytosol like a plasmid. <br /></p> | <td valign="top"><font size="3" face="Verdana" color="#333"><p>When a normal M13 phage infect <em>E.coli</em><em> with </em>F plasmid(strain JM101 in our experiment), it will use F pilus to put its genome into cytosol. Then this single strand genome will use host’s polymerase to make itself a double strand structure and stay in cytosol like a plasmid. <br /></p> |
Revision as of 15:07, 17 October 2014
M13 Phage |
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M13 Phage Mechanism | |||||
In our project, the CRISPR system is been transported by phage. So how can phage recognized which DNA it should package and spread? That is accessed by phagemid and helper phage. |
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Take M13KO7 helper phage and phagemid pBluescript II SK(-) as example, as we use them in our experiment. M13KO7 helper phage has complete coat proteins and a complete genome just like normal M13 phage. But its f1 ori has been inserted by a p15A ori and a kanamycin resistance gene. While in pBluescript, its structure is like a normal plasmid but carries two replication origin: a high efficient PUC ori for itself, and an additional normal f1 ori. |
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