Team:INSA-Lyon/Results

From 2014.igem.org

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Despite not having any specific Nickel-binding motifs, <b>the part K1404006 can chelate small amounts of nickel</b>.
 
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<p> Nickel(II) chelation was evaluated in a CsgA- MG1655 background (in order to have only our modified or unmodified curlis at the surface of the strain) for each of the constructions (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404007">BBa_K1404007</a>,or <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404008">BBa_K1404008</a>). Dimethylglyoxime (DMG) was used as a complexing reagent, which forms a pink-colored complex (peak absorption at 554nm) in the presence of Ni(II). </p>
<p> Nickel(II) chelation was evaluated in a CsgA- MG1655 background (in order to have only our modified or unmodified curlis at the surface of the strain) for each of the constructions (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404006">BBa_K1404006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404007">BBa_K1404007</a>,or <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1404008">BBa_K1404008</a>). Dimethylglyoxime (DMG) was used as a complexing reagent, which forms a pink-colored complex (peak absorption at 554nm) in the presence of Ni(II). </p>

Revision as of 13:43, 17 October 2014

Curly'on - IGEM 2014 INSA-LYON

  • Curli characterization


  • Nickel chelation


  • Survival after UV and high temperature exposure


  • Promoter optimization and characterization


  • Retrieved from "http://2014.igem.org/Team:INSA-Lyon/Results"